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Protein

Rho GTPase-activating protein 8

Gene

ARHGAP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

  • GTPase activator activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 8
Alternative name(s):
Rho-type GTPase-activating protein 8
Gene namesi
Name:ARHGAP8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:677. ARHGAP8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24961.

Polymorphism and mutation databases

BioMutaiARHGAP8.
DMDMi160016276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Rho GTPase-activating protein 8PRO_0000308161Add
BLAST

Proteomic databases

MaxQBiP85298.
PaxDbiP85298.
PRIDEiP85298.

PTM databases

iPTMnetiP85298.
PhosphoSiteiP85298.

Expressioni

Tissue specificityi

Highly expressed in kidney and placenta. Also expressed in colon, skeletal muscle, small intestine, stomach, and testis. Not detected in brain, liver or spleen. Overexpressed in the majority of colorectal tumors examined.1 Publication

Gene expression databases

BgeeiP85298.
ExpressionAtlasiP85298. baseline and differential.
GenevisibleiP85298. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Map2k2Q639325EBI-9523517,EBI-397724From a different organism.
PIN1Q1352625EBI-9523517,EBI-714158

Protein-protein interaction databases

BioGridi117278. 7 interactions.
IntActiP85298. 2 interactions.
STRINGi9606.ENSP00000262731.

Structurei

3D structure databases

ProteinModelPortaliP85298.
SMRiP85298. Positions 215-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 199187CRAL-TRIOPROSITE-ProRule annotationAdd
BLAST
Domaini226 – 412187Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiP85298.
OrthoDBiEOG7JMGD7.
PhylomeDBiP85298.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 2 hits.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 2 hits.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P85298-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD
60 70 80 90 100
HQRLLEYLKY TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK
110 120 130 140 150
DGDLTMWPRL VSNSKLKRSS HLSLPKYWDY RYKKNLKALY VVHPTSFIKV
160 170 180 190 200
LWNILKPLIS HKFGKKVIYF NYLSELHEHL KYDQLVIPPE VLRYDEKLQS
210 220 230 240 250
LHEGRTPPPT KTPPPRPPLP TQQFGVSLQY LKDKNQGELI PPVLRFTVTY
260 270 280 290 300
LREKGLRTEG LFRRSASVQT VREIQRLYNQ GKPVNFDDYG DIHIPAVILK
310 320 330 340 350
TFLRELPQPL LTFQAYEQIL GITCVESSLR VTGCRQILRS LPEHNYVVLR
360 370 380 390 400
YLMGFLHAVS RESIFNKMNS SNLACVFGLN LIWPSQGVSS LSALVPLNMF
410 420 430 440 450
TELLIEYYEK IFSTPEAPGE HGLAPWEQGS RAAPLQEAVP RTQATGLTKP
460
TLPPSPLMAA RRRL
Length:464
Mass (Da):53,484
Last modified:October 23, 2007 - v1
Checksum:iF003A341AE5271D2
GO
Isoform 2 (identifier: P85298-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.
     325-325: V → A
     326-464: Missing.

Show »
Length:294
Mass (Da):34,305
Checksum:i2C09660FED93A2E7
GO
Isoform 3 (identifier: P85298-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     127-131: Missing.

Show »
Length:459
Mass (Da):52,700
Checksum:i4CCBB7E324CE1192
GO
Isoform 4 (identifier: P85298-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):49,752
Checksum:i0AE4B430EAEBF0D3
GO
Isoform 5 (identifier: P85298-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.
     326-336: ESSLRVTGCRQ → PGEHLQQNEQL
     337-464: Missing.

Show »
Length:305
Mass (Da):35,607
Checksum:iC6E7729091A97E39
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti172 – 1721Y → C.
Corresponds to variant rs16992915 [ dbSNP | Ensembl ].
VAR_049138
Natural varianti333 – 3331G → R.
Corresponds to variant rs6007344 [ dbSNP | Ensembl ].
VAR_049139
Natural varianti415 – 4151P → L.
Corresponds to variant rs2071762 [ dbSNP | Ensembl ].
VAR_049140
Natural varianti418 – 4181P → R.
Corresponds to variant rs9614957 [ dbSNP | Ensembl ].
VAR_061182

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei101 – 13131Missing in isoform 2, isoform 4 and isoform 5. 2 PublicationsVSP_001652Add
BLAST
Alternative sequencei127 – 1315Missing in isoform 3. CuratedVSP_028883
Alternative sequencei325 – 3251V → A in isoform 2. 1 PublicationVSP_001656
Alternative sequencei326 – 464139Missing in isoform 2. 1 PublicationVSP_001657Add
BLAST
Alternative sequencei326 – 33611ESSLRVTGCRQ → PGEHLQQNEQL in isoform 5. 1 PublicationVSP_054100Add
BLAST
Alternative sequencei337 – 464128Missing in isoform 5. 1 PublicationVSP_054101Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001306 mRNA. Translation: BAA91614.1.
AK022305 mRNA. Translation: BAB14008.1.
Z83838 Genomic DNA. No translation available.
Z93244 Genomic DNA. No translation available.
Z98743 Genomic DNA. No translation available.
BC067824 mRNA. No translation available.
CCDSiCCDS14060.2. [P85298-4]
CCDS33664.1.
CCDS56233.1. [P85298-5]
PIRiB59436.
RefSeqiNP_001017526.1. NM_001017526.1. [P85298-1]
NP_001185655.1. NM_001198726.1. [P85298-5]
NP_851852.2. NM_181335.2. [P85298-4]
UniGeneiHs.102336.

Genome annotation databases

EnsembliENST00000336963; ENSP00000337287; ENSG00000241484. [P85298-5]
ENST00000356099; ENSP00000348407; ENSG00000241484. [P85298-4]
ENST00000389774; ENSP00000374424; ENSG00000241484. [P85298-1]
GeneIDi23779.
KEGGihsa:23779.
UCSCiuc003bfj.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001306 mRNA. Translation: BAA91614.1.
AK022305 mRNA. Translation: BAB14008.1.
Z83838 Genomic DNA. No translation available.
Z93244 Genomic DNA. No translation available.
Z98743 Genomic DNA. No translation available.
BC067824 mRNA. No translation available.
CCDSiCCDS14060.2. [P85298-4]
CCDS33664.1.
CCDS56233.1. [P85298-5]
PIRiB59436.
RefSeqiNP_001017526.1. NM_001017526.1. [P85298-1]
NP_001185655.1. NM_001198726.1. [P85298-5]
NP_851852.2. NM_181335.2. [P85298-4]
UniGeneiHs.102336.

3D structure databases

ProteinModelPortaliP85298.
SMRiP85298. Positions 215-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117278. 7 interactions.
IntActiP85298. 2 interactions.
STRINGi9606.ENSP00000262731.

PTM databases

iPTMnetiP85298.
PhosphoSiteiP85298.

Polymorphism and mutation databases

BioMutaiARHGAP8.
DMDMi160016276.

Proteomic databases

MaxQBiP85298.
PaxDbiP85298.
PRIDEiP85298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336963; ENSP00000337287; ENSG00000241484. [P85298-5]
ENST00000356099; ENSP00000348407; ENSG00000241484. [P85298-4]
ENST00000389774; ENSP00000374424; ENSG00000241484. [P85298-1]
GeneIDi23779.
KEGGihsa:23779.
UCSCiuc003bfj.3. human.

Organism-specific databases

CTDi23779.
GeneCardsiARHGAP8.
HGNCiHGNC:677. ARHGAP8.
MIMi609405. gene.
neXtProtiNX_P85298.
PharmGKBiPA24961.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiP85298.
OrthoDBiEOG7JMGD7.
PhylomeDBiP85298.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiARHGAP8. human.
GeneWikiiARHGAP8.
GenomeRNAii23779.
NextBioi46765.
PROiP85298.
SOURCEiSearch...

Gene expression databases

BgeeiP85298.
ExpressionAtlasiP85298. baseline and differential.
GenevisibleiP85298. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 2 hits.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 2 hits.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary gland and Teratocarcinoma.
  2. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
  4. "ARHGAP8 is a novel member of the RHOGAP family related to ARHGAP1/CDC42GAP/p50RHOGAP: mutation and expression analyses in colorectal and breast cancers."
    Johnstone C.N., Castellvi-Bel S., Chang L.M., Bessa X., Nakagawa H., Harada H., Sung R.K., Pique J.M., Castells A., Rustgi A.K.
    Gene 336:59-71(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.

Entry informationi

Entry nameiRHG08_HUMAN
AccessioniPrimary (citable) accession number: P85298
Secondary accession number(s): A6ZJ79
, A6ZJ80, O75983, O95695, Q96RW1, Q96RW2, Q9HA49, Q9HC46, Q9NSG0, Q9NVX8, Q9NXL1, Q9UH20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: March 16, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.