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Protein

Rho GTPase-activating protein 8

Gene

ARHGAP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

  • GTPase activator activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 8
Alternative name(s):
Rho-type GTPase-activating protein 8
Gene namesi
Name:ARHGAP8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:677. ARHGAP8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi23779.
OpenTargetsiENSG00000241484.
PharmGKBiPA24961.

Polymorphism and mutation databases

BioMutaiARHGAP8.
DMDMi160016276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003081611 – 464Rho GTPase-activating protein 8Add BLAST464

Proteomic databases

MaxQBiP85298.
PaxDbiP85298.
PeptideAtlasiP85298.
PRIDEiP85298.

PTM databases

iPTMnetiP85298.
PhosphoSitePlusiP85298.

Expressioni

Tissue specificityi

Highly expressed in kidney and placenta. Also expressed in colon, skeletal muscle, small intestine, stomach, and testis. Not detected in brain, liver or spleen. Overexpressed in the majority of colorectal tumors examined.1 Publication

Gene expression databases

BgeeiENSG00000241484.
ExpressionAtlasiP85298. baseline and differential.
GenevisibleiP85298. HS.

Organism-specific databases

HPAiHPA069694.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Map2k2Q639325EBI-9523517,EBI-397724From a different organism.
PIN1Q1352625EBI-9523517,EBI-714158

Protein-protein interaction databases

BioGridi117278. 7 interactors.
IntActiP85298. 2 interactors.
STRINGi9606.ENSP00000262731.

Structurei

3D structure databases

ProteinModelPortaliP85298.
SMRiP85298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 199CRAL-TRIOPROSITE-ProRule annotationAdd BLAST187
Domaini226 – 412Rho-GAPPROSITE-ProRule annotationAdd BLAST187

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00420000029688.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiP85298.
KOiK20633.
OrthoDBiEOG091G07HT.
PhylomeDBiP85298.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 2 hits.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 2 hits.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P85298-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD
60 70 80 90 100
HQRLLEYLKY TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK
110 120 130 140 150
DGDLTMWPRL VSNSKLKRSS HLSLPKYWDY RYKKNLKALY VVHPTSFIKV
160 170 180 190 200
LWNILKPLIS HKFGKKVIYF NYLSELHEHL KYDQLVIPPE VLRYDEKLQS
210 220 230 240 250
LHEGRTPPPT KTPPPRPPLP TQQFGVSLQY LKDKNQGELI PPVLRFTVTY
260 270 280 290 300
LREKGLRTEG LFRRSASVQT VREIQRLYNQ GKPVNFDDYG DIHIPAVILK
310 320 330 340 350
TFLRELPQPL LTFQAYEQIL GITCVESSLR VTGCRQILRS LPEHNYVVLR
360 370 380 390 400
YLMGFLHAVS RESIFNKMNS SNLACVFGLN LIWPSQGVSS LSALVPLNMF
410 420 430 440 450
TELLIEYYEK IFSTPEAPGE HGLAPWEQGS RAAPLQEAVP RTQATGLTKP
460
TLPPSPLMAA RRRL
Length:464
Mass (Da):53,484
Last modified:October 23, 2007 - v1
Checksum:iF003A341AE5271D2
GO
Isoform 2 (identifier: P85298-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.
     325-325: V → A
     326-464: Missing.

Show »
Length:294
Mass (Da):34,305
Checksum:i2C09660FED93A2E7
GO
Isoform 3 (identifier: P85298-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     127-131: Missing.

Show »
Length:459
Mass (Da):52,700
Checksum:i4CCBB7E324CE1192
GO
Isoform 4 (identifier: P85298-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):49,752
Checksum:i0AE4B430EAEBF0D3
GO
Isoform 5 (identifier: P85298-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.
     326-336: ESSLRVTGCRQ → PGEHLQQNEQL
     337-464: Missing.

Show »
Length:305
Mass (Da):35,607
Checksum:iC6E7729091A97E39
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049138172Y → C.Corresponds to variant rs16992915dbSNPEnsembl.1
Natural variantiVAR_049139333G → R.Corresponds to variant rs6007344dbSNPEnsembl.1
Natural variantiVAR_049140415P → L.Corresponds to variant rs2071762dbSNPEnsembl.1
Natural variantiVAR_061182418P → R.Corresponds to variant rs9614957dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001652101 – 131Missing in isoform 2, isoform 4 and isoform 5. 2 PublicationsAdd BLAST31
Alternative sequenceiVSP_028883127 – 131Missing in isoform 3. Curated5
Alternative sequenceiVSP_001656325V → A in isoform 2. 1 Publication1
Alternative sequenceiVSP_001657326 – 464Missing in isoform 2. 1 PublicationAdd BLAST139
Alternative sequenceiVSP_054100326 – 336ESSLRVTGCRQ → PGEHLQQNEQL in isoform 5. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_054101337 – 464Missing in isoform 5. 1 PublicationAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001306 mRNA. Translation: BAA91614.1.
AK022305 mRNA. Translation: BAB14008.1.
Z83838 Genomic DNA. No translation available.
Z93244 Genomic DNA. No translation available.
Z98743 Genomic DNA. No translation available.
BC067824 mRNA. No translation available.
CCDSiCCDS14060.2. [P85298-4]
CCDS33664.1.
CCDS56233.1. [P85298-5]
PIRiB59436.
RefSeqiNP_001017526.1. NM_001017526.1. [P85298-1]
NP_001185655.1. NM_001198726.1. [P85298-5]
NP_851852.2. NM_181335.2. [P85298-4]
UniGeneiHs.102336.

Genome annotation databases

EnsembliENST00000336963; ENSP00000337287; ENSG00000241484. [P85298-5]
ENST00000356099; ENSP00000348407; ENSG00000241484. [P85298-4]
ENST00000389774; ENSP00000374424; ENSG00000241484. [P85298-1]
GeneIDi23779.
KEGGihsa:23779.
UCSCiuc003bfj.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001306 mRNA. Translation: BAA91614.1.
AK022305 mRNA. Translation: BAB14008.1.
Z83838 Genomic DNA. No translation available.
Z93244 Genomic DNA. No translation available.
Z98743 Genomic DNA. No translation available.
BC067824 mRNA. No translation available.
CCDSiCCDS14060.2. [P85298-4]
CCDS33664.1.
CCDS56233.1. [P85298-5]
PIRiB59436.
RefSeqiNP_001017526.1. NM_001017526.1. [P85298-1]
NP_001185655.1. NM_001198726.1. [P85298-5]
NP_851852.2. NM_181335.2. [P85298-4]
UniGeneiHs.102336.

3D structure databases

ProteinModelPortaliP85298.
SMRiP85298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117278. 7 interactors.
IntActiP85298. 2 interactors.
STRINGi9606.ENSP00000262731.

PTM databases

iPTMnetiP85298.
PhosphoSitePlusiP85298.

Polymorphism and mutation databases

BioMutaiARHGAP8.
DMDMi160016276.

Proteomic databases

MaxQBiP85298.
PaxDbiP85298.
PeptideAtlasiP85298.
PRIDEiP85298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336963; ENSP00000337287; ENSG00000241484. [P85298-5]
ENST00000356099; ENSP00000348407; ENSG00000241484. [P85298-4]
ENST00000389774; ENSP00000374424; ENSG00000241484. [P85298-1]
GeneIDi23779.
KEGGihsa:23779.
UCSCiuc003bfj.3. human.

Organism-specific databases

CTDi23779.
DisGeNETi23779.
GeneCardsiARHGAP8.
HGNCiHGNC:677. ARHGAP8.
HPAiHPA069694.
MIMi609405. gene.
neXtProtiNX_P85298.
OpenTargetsiENSG00000241484.
PharmGKBiPA24961.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00420000029688.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiP85298.
KOiK20633.
OrthoDBiEOG091G07HT.
PhylomeDBiP85298.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiARHGAP8. human.
GeneWikiiARHGAP8.
GenomeRNAii23779.
PROiP85298.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000241484.
ExpressionAtlasiP85298. baseline and differential.
GenevisibleiP85298. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 2 hits.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 2 hits.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG08_HUMAN
AccessioniPrimary (citable) accession number: P85298
Secondary accession number(s): A6ZJ79
, A6ZJ80, O75983, O95695, Q96RW1, Q96RW2, Q9HA49, Q9HC46, Q9NSG0, Q9NVX8, Q9NXL1, Q9UH20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.