P85298 (RHG08_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho GTPase-activating protein 8

Alternative name(s):
Rho-type GTPase-activating protein 8

Gene names

Name:

ARHGAP8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity.
Tissue specificity	Highly expressed in kidney and placenta. Also expressed in colon, skeletal muscle, small intestine, stomach, and testis. Not detected in brain, liver or spleen. Overexpressed in the majority of colorectal tumors examined. Ref.3
Sequence similarities	Contains 1 CRAL-TRIO domain. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	GTPase activation
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of ERK1 and ERK2 cascade Inferred from direct assay. Source: BHF-UCL small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	Rho GTPase activator activity Inferred from direct assay. Source: BHF-UCL protein binding Inferred from physical interaction. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P85298-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P85298-2)

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.
     325-325: V → A
     326-464: Missing.

Isoform 3 (identifier: P85298-3)

The sequence of this isoform differs from the canonical sequence as follows:
     127-131: Missing.

Isoform 4 (identifier: P85298-4)

The sequence of this isoform differs from the canonical sequence as follows:
     101-131: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 464	463	Rho GTPase-activating protein 8		PRO_0000308161
Regions
	Domain	13 – 199	187	CRAL-TRIO
	Domain	226 – 412	187	Rho-GAP
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.5
	Modified residue	448	1	Phosphothreonine Ref.4
Natural variations
	Alternative sequence	101 – 131	31	Missing in isoform 2 and isoform 4.		VSP_001652
	Alternative sequence	127 – 131	5	Missing in isoform 3.		VSP_028883
	Alternative sequence	325	1	V → A in isoform 2.		VSP_001656
	Alternative sequence	326 – 464	139	Missing in isoform 2.		VSP_001657
	Natural variant	172	1	Y → C. Corresponds to variant rs16992915 [ dbSNP | Ensembl ].		VAR_049138
	Natural variant	333	1	G → R. Corresponds to variant rs6007344 [ dbSNP | Ensembl ].		VAR_049139
	Natural variant	415	1	P → L. Corresponds to variant rs2071762 [ dbSNP | Ensembl ].		VAR_049140
	Natural variant	418	1	P → R. Corresponds to variant rs9614957 [ dbSNP | Ensembl ].		VAR_061182

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F003A341AE5271D2	FASTA	464	53,484
	10 20 30 40 50 60 MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY 70 80 90 100 110 120 TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK DGDLTMWPRL VSNSKLKRSS 130 140 150 160 170 180 HLSLPKYWDY RYKKNLKALY VVHPTSFIKV LWNILKPLIS HKFGKKVIYF NYLSELHEHL 190 200 210 220 230 240 KYDQLVIPPE VLRYDEKLQS LHEGRTPPPT KTPPPRPPLP TQQFGVSLQY LKDKNQGELI 250 260 270 280 290 300 PPVLRFTVTY LREKGLRTEG LFRRSASVQT VREIQRLYNQ GKPVNFDDYG DIHIPAVILK 310 320 330 340 350 360 TFLRELPQPL LTFQAYEQIL GITCVESSLR VTGCRQILRS LPEHNYVVLR YLMGFLHAVS 370 380 390 400 410 420 RESIFNKMNS SNLACVFGLN LIWPSQGVSS LSALVPLNMF TELLIEYYEK IFSTPEAPGE 430 440 450 460 HGLAPWEQGS RAAPLQEAVP RTQATGLTKP TLPPSPLMAA RRRL
	Isoform 2 [UniParc] [UniParc]. Checksum: 2C09660FED93A2E7	FASTA	294	34,305
	10 20 30 40 50 60 MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY 70 80 90 100 110 120 TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK YKKNLKALYV VHPTSFIKVL 130 140 150 160 170 180 WNILKPLISH KFGKKVIYFN YLSELHEHLK YDQLVIPPEV LRYDEKLQSL HEGRTPPPTK 190 200 210 220 230 240 TPPPRPPLPT QQFGVSLQYL KDKNQGELIP PVLRFTVTYL REKGLRTEGL FRRSASVQTV 250 260 270 280 290 REIQRLYNQG KPVNFDDYGD IHIPAVILKT FLRELPQPLL TFQAYEQILG ITCA
	Isoform 3 [UniParc]. Checksum: 4CCBB7E324CE1192	FASTA	459	52,700
	10 20 30 40 50 60 MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY 70 80 90 100 110 120 TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK DGDLTMWPRL VSNSKLKRSS 130 140 150 160 170 180 HLSLPKYKKN LKALYVVHPT SFIKVLWNIL KPLISHKFGK KVIYFNYLSE LHEHLKYDQL 190 200 210 220 230 240 VIPPEVLRYD EKLQSLHEGR TPPPTKTPPP RPPLPTQQFG VSLQYLKDKN QGELIPPVLR 250 260 270 280 290 300 FTVTYLREKG LRTEGLFRRS ASVQTVREIQ RLYNQGKPVN FDDYGDIHIP AVILKTFLRE 310 320 330 340 350 360 LPQPLLTFQA YEQILGITCV ESSLRVTGCR QILRSLPEHN YVVLRYLMGF LHAVSRESIF 370 380 390 400 410 420 NKMNSSNLAC VFGLNLIWPS QGVSSLSALV PLNMFTELLI EYYEKIFSTP EAPGEHGLAP 430 440 450 WEQGSRAAPL QEAVPRTQAT GLTKPTLPPS PLMAARRRL
	Isoform 4 [UniParc]. Checksum: 0AE4B430EAEBF0D3	FASTA	433	49,752
	10 20 30 40 50 60 MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY 70 80 90 100 110 120 TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK YKKNLKALYV VHPTSFIKVL 130 140 150 160 170 180 WNILKPLISH KFGKKVIYFN YLSELHEHLK YDQLVIPPEV LRYDEKLQSL HEGRTPPPTK 190 200 210 220 230 240 TPPPRPPLPT QQFGVSLQYL KDKNQGELIP PVLRFTVTYL REKGLRTEGL FRRSASVQTV 250 260 270 280 290 300 REIQRLYNQG KPVNFDDYGD IHIPAVILKT FLRELPQPLL TFQAYEQILG ITCVESSLRV 310 320 330 340 350 360 TGCRQILRSL PEHNYVVLRY LMGFLHAVSR ESIFNKMNSS NLACVFGLNL IWPSQGVSSL 370 380 390 400 410 420 SALVPLNMFT ELLIEYYEKI FSTPEAPGEH GLAPWEQGSR AAPLQEAVPR TQATGLTKPT 430 LPPSPLMAAR RRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Mammary gland and Teratocarcinoma.
[2]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 4).
[3]	"ARHGAP8 is a novel member of the RHOGAP family related to ARHGAP1/CDC42GAP/p50RHOGAP: mutation and expression analyses in colorectal and breast cancers." Johnstone C.N., Castellvi-Bel S., Chang L.M., Bessa X., Nakagawa H., Harada H., Sung R.K., Pique J.M., Castells A., Rustgi A.K. Gene 336:59-71(2004) [PubMed: 15225876] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-448, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001306 mRNA. Translation: BAA91614.1. AK022305 mRNA. Translation: BAB14008.1. Z83838, Z93244 Genomic DNA. Translation: CAI19475.2. Z93244, Z83838 Genomic DNA. Translation: CAI17883.2. Z93244, Z83838 Genomic DNA. Translation: CAO03395.1. Z83838, Z93244 Genomic DNA. Translation: CAO03545.1.
IPI	IPI00216062. IPI00472223. IPI00868745. IPI00983963.
PIR	B59436.
RefSeq	NP_001017526.1. NM_001017526.1. NP_001185655.1. NM_001198726.1. NP_851852.2. NM_181335.2.
UniGene	Hs.102336.
3D structure databases
ProteinModelPortal	P85298.
SMR	P85298. Positions 215-412.
ModBase	Search...
Protein-protein interaction databases
STRING	P85298.
PTM databases
PhosphoSite	P85298.
Polymorphism databases
DMDM	160016276.
Proteomic databases
PRIDE	P85298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000389774; ENSP00000374424; ENSG00000241484.
GeneID	23779.
KEGG	hsa:23779.
UCSC	uc003bfi.2. human. uc003bfj.1. human.
Organism-specific databases
CTD	23779.
GeneCards	GC22P045148.
H-InvDB	HIX0016569.
HGNC	HGNC:677. ARHGAP8.
MIM	609405. gene.
neXtProt	NX_P85298.
PharmGKB	PA24961.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG054433.
InParanoid	P85298.
OMA	ILWNIFK.
OrthoDB	EOG4CNQRX.
PhylomeDB	P85298.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P85298.
Bgee	P85298.
Genevestigator	P85298.
GermOnline	ENSG00000186654. Homo sapiens.
Family and domain databases
InterPro	IPR001251. CRAL-TRIO_dom. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Gene3D	G3DSA:3.40.525.10. CRAL_bd_TRIO_C. 2 hits. G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00650. CRAL_TRIO. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. SM00516. SEC14. 1 hit. [Graphical view]
SUPFAM	SSF52087. CRAL_TRIO_C. 1 hit. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50191. CRAL_TRIO. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	46765.
SOURCE	Search...

Entry information

Entry name

RHG08_HUMAN

Accession

Primary (citable) accession number: P85298
Secondary accession number(s): A6ZJ80 , O75983, O95695, Q96RW1, Q96RW2, Q9HA49, Q9HC46, Q9NSG0, Q9NVX8, Q9NXL1, Q9UH20

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.