P85290 (NDKB_MERBI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase B
Short name=NDK B
Short name=NDP kinase B
EC=2.7.4.6

Gene names

Name:

nme2

Organism

Merluccius bilinearis (Silver hake)

Taxonomic identifier

79698 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Merlucciidae › Merlucciinae › Merluccius

Protein attributes

Sequence length	128 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP By similarity. UniProtKB P15531
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. UniProtKB P15531
Cofactor	Magnesium By similarity. UniProtKB P15531
Subcellular location	Cytoplasm By similarity UniProtKB P15531.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Cell cycle Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Nucleoside diphosphate kinase B		PRO_0000306186
Sites
	Active site	94	1	Pros-phosphohistidine intermediate By similarity UniProtKB P15531
	Binding site	9	1	ATP By similarity UniProtKB P15531
	Binding site	39	1	ATP By similarity UniProtKB P15531
	Binding site	70	1	ATP By similarity UniProtKB P15531
	Binding site	81	1	ATP By similarity UniProtKB P15531
	Binding site	91	1	ATP By similarity UniProtKB P15531
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
Experimental info
	Non-adjacent residues	23 – 24	2
	Non-adjacent residues	27 – 28	2
	Non-adjacent residues	29 – 30	2
	Non-adjacent residues	64 – 65	2

Sequences

	Sequence		Length	Mass (Da)
	P85290 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 0C2A199A17C40E69	FASTA	128	14,407
	10 20 30 40 50 60 MEQTFIAIKP DGVQRGLCGE VMKFIQPMKH YLDLKDMPFY AGLCKYMSSG PVFAMVWEGE 70 80 90 100 110 120 GIVKMMLGET NPADSKPGSI RGDFCINIGR NIIHGSDTVE NAKMEVGLWF KPEEFVAYAE KAKAWVYE

References

[1]

"De novo mass spectrometry sequencing and characterization of species-specific peptides from nucleoside diphosphate kinase B for the classification of commercial fish species belonging to the family Merlucciidae." Carrera M., Canas B., Pineiro C., Vazquez J., Gallardo J.M. J. Proteome Res. 6:3070-3080(2007) [PubMed: 17622168] [Abstract] Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1. Tissue: White muscle.

Cross-references

3D structure databases
ProteinModelPortal	P85290.
SMR	P85290. Positions 2-128.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000423.
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 2 hits.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_MERBI

Accession

Primary (citable) accession number: P85290

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	May 31, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program