P85281 (NDKB_MERCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase B
Short name=NDK B
Short name=NDP kinase B
EC=2.7.4.6

Gene names

Name:

nme2

Organism

Merluccius capensis (Shallow-water Cape hake) (Gadus merluccius)

Taxonomic identifier

89947 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Merlucciidae › Merlucciinae › Merluccius

Protein attributes

Sequence length	126 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP By similarity. UniProtKB P15531
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. UniProtKB P15531
Cofactor	Magnesium By similarity. UniProtKB P15531
Subcellular location	Cytoplasm By similarity UniProtKB P15531.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Cell cycle Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 126	›126	Nucleoside diphosphate kinase B		PRO_0000306187
Sites
	Active site	92	1	Pros-phosphohistidine intermediate By similarity UniProtKB P15531
	Binding site	6	1	ATP By similarity UniProtKB P15531
	Binding site	37	1	ATP By similarity UniProtKB P15531
	Binding site	68	1	ATP By similarity UniProtKB P15531
	Binding site	79	1	ATP By similarity UniProtKB P15531
	Binding site	89	1	ATP By similarity UniProtKB P15531
Experimental info
	Non-adjacent residues	20 – 21	2
	Non-adjacent residues	24 – 25	2
	Non-adjacent residues	62 – 63	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P85281 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 619C9DBA188274CC	FASTA	126	14,207
	10 20 30 40 50 60 TFVAIKPDGV QRGLCGEVMK FIQPMKQHYL DLKDMPFYAG LCKYMSSGPV FAMVWEGEGI 70 80 90 100 110 120 VKMMLGETNP ADSKPGSIRG DFCINIGRNI IHGSDTVENA KMEVALWFKP EEFVTYTEKA KAWVYE

References

[1]

"De novo mass spectrometry sequencing and characterization of species-specific peptides from nucleoside diphosphate kinase B for the classification of commercial fish species belonging to the family Merlucciidae." Carrera M., Canas B., Pineiro C., Vazquez J., Gallardo J.M. J. Proteome Res. 6:3070-3080(2007) [PubMed: 17622168] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: White muscle.

Cross-references

3D structure databases
ProteinModelPortal	P85281.
SMR	P85281. Positions 1-126.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000423.
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 2 hits.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_MERCP

Accession

Primary (citable) accession number: P85281

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	May 31, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program