Reviewed, UniProtKB/Swiss-Prot P85202 (CMA1_RABIT)

Names and origin

Protein names

Recommended name:
    Chymase
    EC=3.4.21.39
Alternative name(s):
    Alpha-chymase

Gene names

Name:

CMA1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion By similarity. UniProtKB P23946
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa. UniProtKB P23946
Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity. UniProtKB P23946
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Chymase		PRO_0000312844
Regions
	Domain	2 – 218	217	Peptidase S1
Sites
	Active site	46	1	Charge relay system By similarity UniProtKB P23946
	Active site	90	1	Charge relay system By similarity UniProtKB P23946
	Active site	177	1	Charge relay system By similarity UniProtKB P23946
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 47		By similarity UniProtKB P23946
	Disulfide bond	124 ↔ 182		By similarity UniProtKB P23946
	Disulfide bond	155 ↔ 168		By similarity UniProtKB P23946

Sequences

	Sequence		Length	Mass (Da)
	P85202-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: D6E7B8C87D57156E	FASTA	220	24,710
	10 20 30 40 50 60 EIIGGTESKP HSRPYMAHLK IITKQGTFAY CGGFLISREF VMTAAHCKGR HITVTLGAHD 70 80 90 100 110 120 VVKKESTWQK IDVVKQFVHP NYNSYTIRHD VMLLKLKEKA NLTLTVGTLP LLPQSNFIPP 130 140 150 160 170 180 GRMCRAVGWG KTSVKESHSN VLQEVKLRLL DPPACQHFPD FNHNLQLCMG TVFKGDSGPL 190 200 210 220 LCARVAQGIA SYAHKNAMPP SVFTRISYYR PWINKVLKEN

References

[1]

"Structural basis for elastolytic substrate specificity in rodent alpha-chymases." Kervinen J., Abad M., Crysler C., Kolpak M., Mahan A.D., Masucci J.A., Bayoumy S., Cummings M.D., Yao X., Olson M., de Garavilla L., Kuo L., Deckman I., Spurlino J. J. Biol. Chem. 283:427-436(2008) [PubMed: 17981788] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P85202.
Phylogenomic databases
OMA	RKTKSAF.
Enzyme and pathway databases
BRENDA	3.4.21.39. 255.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMA1_RABIT

Accession

Primary (citable) accession number: P85202

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)