Reviewed, UniProtKB/Swiss-Prot P85149 (LAC2_CERUI)

Names and origin

Protein names	Recommended name:     Laccase-2     EC=1.10.3.2 Alternative name(s):     Benzenediol:oxygen oxidoreductase 2     Urishiol oxidase 2     Diphenol oxidase 2     Lac II
Organism	Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifier	90312 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Cerrena

Protein attributes

Sequence length	10 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable. UniProtKB Q12718
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O. Ref.1
Cofactor	Binds 4 copper ions per monomer By similarity. UniProtKB Q12718
Subcellular location	Secreted By similarity UniProtKB Q12718.
Post-translational modification	Glycosylated; contains 8.47% carbohydrates. Ref.1
Miscellaneous	On the 2D-gel the determined pI of this protein is: 4.56, its MW is: 62.03 kDa. Ref.1
Sequence similarities	Belongs to the multicopper oxidase family.
Biophysicochemical properties	Kinetic parameters: KM=0.166 mM for syringaldazine Ref.1 pH dependence: Optimum pH is 5.3. Ref.1 Temperature dependence: Optimum temperature is 60 degrees Celsius. Ref.1

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›10	›10	Laccase-2		PRO_0000288803
Experimental info
	Non-terminal residue	10	1

Sequences

	Sequence		Length	Mass (Da)
	P85149-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 212CE22AADC2D768	FASTA	10	1,005
	10 AIGPVADLHI

References

[1]	"Purification of Cerrena unicolor laccase." Janusz G., Rogalski J.M. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.3.2. 280017.
Family and domain databases
PROSITE	PS00079. MULTICOPPER_OXIDASE1. Partial match. PS00080. MULTICOPPER_OXIDASE2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC2_CERUI

Accession

Primary (citable) accession number: P85149

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	November 24, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)