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P85107

- TGS1_RAT

UniProt

P85107 - TGS1_RAT

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Protein

Trimethylguanosine synthase

Gene

Tgs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,7)G(5')pppR-RNA.
S-adenosyl-L-methionine + m(2,7)G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(2,2,7)G(5')pppR-RNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei713 – 7131S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. RNA trimethylguanosine synthase activity Source: Ensembl

GO - Biological processi

  1. 7-methylguanosine RNA capping Source: InterPro
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_196408. PPARA activates gene expression.
REACT_196852. REV-ERBA represses gene expression.
REACT_196856. RORA activates circadian gene expression.
REACT_198613. Activation of gene expression by SREBF (SREBP).
REACT_198793. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_216049. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylguanosine synthase (EC:2.1.1.-)
Alternative name(s):
Nuclear receptor coactivator 6-interacting protein
PRIP-interacting protein with methyltransferase motif
Short name:
PIMT
Short name:
PIPMT
Gene namesi
Name:Tgs1By similarity
Synonyms:Ncoa6ipImported, PimtBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi1309953. Tgs1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular space Source: Ensembl
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 850850Trimethylguanosine synthasePRO_0000283727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611PhosphothreonineBy similarity
Modified residuei152 – 1521PhosphoserineBy similarity
Modified residuei431 – 4311PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP85107.
PRIDEiP85107.

PTM databases

PhosphoSiteiP85107.

Expressioni

Tissue specificityi

A 55 kDa isoform is widely expressed while a 90 kDa isoform is detected exclusively in brain and testis (at protein level).1 Publication

Gene expression databases

GenevestigatoriP85107.

Interactioni

Subunit structurei

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010783.

Structurei

3D structure databases

ProteinModelPortaliP85107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi611 – 62414Lys-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
GeneTreeiENSGT00390000018056.
HOGENOMiHOG000154561.
HOVERGENiHBG059797.
InParanoidiP85107.
KOiK14292.
OMAiCSRAFVE.
OrthoDBiEOG70CR73.
PhylomeDBiP85107.
TreeFamiTF313065.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF09445. Methyltransf_15. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P85107-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCCEKWSHVA EMLLFIEDRE EEYKILCLCS RAFVEDRKLY NLGLKGYYVK
60 70 80 90 100
SSGNNAGDRV TEEEEDDHSS GTTESHSADE GDLDPEAKLM RSMGLPVRFG
110 120 130 140 150
RMSTHGSFEV SMNARNKAKV RQKRRKHRKQ YLDEIVREDW RNDCEEDDLV
160 170 180 190 200
VSDDPSSVEH CENNSACEIQ SKTDAEAENL PVENTLAPKL EVTENWEKYW
210 220 230 240 250
NEYGEGLLWQ SWQEKYPDQT LSSEPWNLPD TKEEWEQHYS QLYWYYLEQF
260 270 280 290 300
QYWEAQGWTF VASQNCDKDV CTSHTQVDQN AESSLKADGT TLSSSPNTAE
310 320 330 340 350
DESLGSNDND HNEIIAGINK ITLSAEEVEQ SQLDSSEHCD KPLSEVTGKE
360 370 380 390 400
CPASGGSDSC HGTPKESDIS ENRSSDQPAQ ELQESSGTNT SKHRPHHNGT
410 420 430 440 450
DGNESEDDPP DHKPSKMKRS HELDIDENPD SEVDDNGFHL GFKHGSGQKY
460 470 480 490 500
GGIPNFSRRQ VRYLEKNVKY KSKYLDMRKR MTVKNKHIVF SEESGKPFIR
510 520 530 540 550
KSKVRSKVEK FLKWVNEPVD EEISQEPLSH NKMQDTCTSS DSEEQDMSME
560 570 580 590 600
KTDGLMETRD PEPENCQTIS SASELEAEKS EGGSLVAAVP ENCSTEGVAN
610 620 630 640 650
SPRAEAEVEI KKKKKKKKKN KNKKINGLPP EIASVPELAK YWAQRYRLFS
660 670 680 690 700
RFDDGIKLDK EGWFSVTPEK IAEHIAGRVS QSFNCDIIVD AFCGVGGNTI
710 720 730 740 750
QFALTGKRVI AIDIDPVKID LARNNAEVYG VADKIEFICG DFLLLAPCLK
760 770 780 790 800
ADVVFLSPPW GGPDYATAET FDIRTMMSPD GFEIFRLSQK ITNNIVYFLP
810 820 830 840 850
RNADVDQVAS LAGPGGQVEI EQNFLNNKLK TITAYFGDLI RRPALRSAEA
Length:850
Mass (Da):96,045
Last modified:April 3, 2007 - v1
Checksum:i1C47E614F90382DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03040398 Genomic DNA. No translation available.
RefSeqiNP_001101374.1. NM_001107904.1.
UniGeneiRn.48378.

Genome annotation databases

EnsembliENSRNOT00000010783; ENSRNOP00000010783; ENSRNOG00000008156.
GeneIDi312947.
KEGGirno:312947.
UCSCiRGD:1309953. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03040398 Genomic DNA. No translation available.
RefSeqi NP_001101374.1. NM_001107904.1.
UniGenei Rn.48378.

3D structure databases

ProteinModelPortali P85107.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000010783.

PTM databases

PhosphoSitei P85107.

Proteomic databases

PaxDbi P85107.
PRIDEi P85107.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010783 ; ENSRNOP00000010783 ; ENSRNOG00000008156 .
GeneIDi 312947.
KEGGi rno:312947.
UCSCi RGD:1309953. rat.

Organism-specific databases

CTDi 96764.
RGDi 1309953. Tgs1.

Phylogenomic databases

eggNOGi COG0500.
GeneTreei ENSGT00390000018056.
HOGENOMi HOG000154561.
HOVERGENi HBG059797.
InParanoidi P85107.
KOi K14292.
OMAi CSRAFVE.
OrthoDBi EOG70CR73.
PhylomeDBi P85107.
TreeFami TF313065.

Enzyme and pathway databases

Reactomei REACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_196408. PPARA activates gene expression.
REACT_196852. REV-ERBA represses gene expression.
REACT_196856. RORA activates circadian gene expression.
REACT_198613. Activation of gene expression by SREBF (SREBP).
REACT_198793. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_216049. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

NextBioi 665415.
PROi P85107.

Gene expression databases

Genevestigatori P85107.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR019012. RNA_cap_Gua-N2-MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF09445. Methyltransf_15. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway1 Publication.
  2. "Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localize to the cytoplasm and nucleus."
    Enuenlue I., Papai G., Cserpan I., Udvardy A., Jeang K.-T., Boros I.
    Biochem. Biophys. Res. Commun. 309:44-51(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiTGS1_RAT
AccessioniPrimary (citable) accession number: P85107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3