Reviewed, UniProtKB/Swiss-Prot P85101 (RIPLY_LYCCH)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein lychnin     EC=3.2.2.22
Organism	Lychnis chalcedonica (Scarlet lychnis) (Maltese cross)
Taxonomic identifier	39855 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Lychnis

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Inhibits cell-free translation in rabbit reticulocyte lysate system with an IC50 of 0.17 nM. Ref.2 Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Mass spectrometry	Molecular mass is 26131.14±0.46 Da from positions 1 - 234. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Ribosome-inactivating protein lychnin		PRO_0000283747
Sites
	Active site	170	1	By similarity UniProtKB P10297
Amino acid modifications
	Disulfide bond	32 ↔ 115		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P85101-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 3F8B2CBD614EB80F	FASTA	234	26,132
	10 20 30 40 50 60 RPSWTVDSDS AKYSSFLDSL REEFGRGTPK VCNIPVTKKA NNDKFVLVNL VLPFNRNTIT 70 80 90 100 110 120 LAFRASDAYL VGFQDRDSKT NKLRANFFSD EYRALSGKYK SIFTDAEVLA PALPCASTYT 130 140 150 160 170 180 DLQNKAGVSR EKLSLGVSSL QTAFTAVYGK VFTGKNVAKF ALISIQMVAE AARFKYIEDQ 190 200 210 220 230 VINRGMYSSF EAGARITLLE NNWSKISEQY HKSCKLGGGQ FTEEEMKLGL LLYN

References

[1]	"Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds." Chambery A., de Donato A., Bolognesi A., Polito L., Stirpe F., Parente A. Biol. Chem. 387:1261-1266(2006) [PubMed: 16972795] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, DISULFIDE BOND. Tissue: Seed.
[2]	"Purification and properties of new ribosome-inactivating proteins with RNA N-glycosidase activity." Bolognesi A., Barbieri L., Abbondanza A., Falasca A.I., Carnicelli D., Battelli M.G., Stirpe F. Biochim. Biophys. Acta 1087:293-302(1990) [PubMed: 2248976] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G5X	X-ray	1.70	A	1-234	[»]

SMR

P85101. Positions 1-234.

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 309419.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIPLY_LYCCH

Accession

Primary (citable) accession number: P85101

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)