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Protein

Ribosome-inactivating protein lychnin

Gene
N/A
Organism
Silene chalcedonica (Maltese-cross) (Lychnis chalcedonica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Inhibits cell-free translation in rabbit reticulocyte lysate system with an IC50 of 0.17 nM.1 Publication

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170By similarity1

GO - Molecular functioni

  • RNA glycosylase activity Source: UniProtKB
  • rRNA N-glycosylase activity Source: UniProtKB
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein synthesis inhibitor, Toxin
Biological processPlant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-inactivating protein lychnin (EC:3.2.2.22)
OrganismiSilene chalcedonica (Maltese-cross) (Lychnis chalcedonica)
Taxonomic identifieri39855 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeSileneaeSilene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002837471 – 234Ribosome-inactivating protein lychninAdd BLAST234

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 1151 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP85101

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G5XX-ray1.70A1-234[»]
ProteinModelPortaliP85101
SMRiP85101
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP85101

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.420.10, 1 hit
4.10.470.10, 1 hit
InterProiView protein in InterPro
IPR036041 Ribosome-inact_prot_sf
IPR017989 Ribosome_inactivat_1/2
IPR001574 Ribosome_inactivat_prot
IPR017988 Ribosome_inactivat_prot_CS
IPR016138 Ribosome_inactivat_prot_sub1
IPR016139 Ribosome_inactivat_prot_sub2
PfamiView protein in Pfam
PF00161 RIP, 1 hit
PRINTSiPR00396 SHIGARICIN
SUPFAMiSSF56371 SSF56371, 1 hit
PROSITEiView protein in PROSITE
PS00275 SHIGA_RICIN, 1 hit

Sequencei

Sequence statusi: Complete.

P85101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RPSWTVDSDS AKYSSFLDSL REEFGRGTPK VCNIPVTKKA NNDKFVLVNL
60 70 80 90 100
VLPFNRNTIT LAFRASDAYL VGFQDRDSKT NKLRANFFSD EYRALSGKYK
110 120 130 140 150
SIFTDAEVLA PALPCASTYT DLQNKAGVSR EKLSLGVSSL QTAFTAVYGK
160 170 180 190 200
VFTGKNVAKF ALISIQMVAE AARFKYIEDQ VINRGMYSSF EAGARITLLE
210 220 230
NNWSKISEQY HKSCKLGGGQ FTEEEMKLGL LLYN
Length:234
Mass (Da):26,132
Last modified:April 3, 2007 - v1
Checksum:i3F8B2CBD614EB80F
GO

Mass spectrometryi

Molecular mass is 26131.14±0.46 Da from positions 1 - 234. Determined by ESI. 1 Publication

Entry informationi

Entry nameiRIPLY_SILCH
AccessioniPrimary (citable) accession number: P85101
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: May 23, 2018
This is version 42 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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