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P85098

- NARH_BRASZ

UniProt

P85098 - NARH_BRASZ

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Protein

Respiratory nitrate reductase beta chain

Gene
narH
Organism
Bradyrhizobium sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.By similarity1 Publication

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.1 Publication

Cofactori

Binds 3 4Fe-4S clusters per subunit By similarity.By similarity
Binds 1 3Fe-4S cluster per subunit By similarity.By similarity

Enzyme regulationi

Inhibited by micromolar concentrations of azide.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S) By similarityBy similarity
Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S) By similarityBy similarity
Metal bindingi18 – 181Iron-sulfur 1 (4Fe-4S) By similarityBy similarity
Metal bindingi22 – 221Iron-sulfur 2 (4Fe-4S) By similarityBy similarity
Metal bindingi39 – 391Iron-sulfur 3 (4Fe-4S) By similarityBy similarity
Metal bindingi42 – 421Iron-sulfur 3 (4Fe-4S) By similarityBy similarity
Metal bindingi47 – 471Iron-sulfur 3 (4Fe-4S) By similarityBy similarity
Metal bindingi51 – 511Iron-sulfur 4 (3Fe-4S) By similarityBy similarity
Metal bindingi73 – 731Iron-sulfur 4 (3Fe-4S) By similarityBy similarity
Metal bindingi77 – 771Iron-sulfur 3 (4Fe-4S) By similarityBy similarity
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S) By similarityBy similarity
Metal bindingi84 – 841Iron-sulfur 2 (4Fe-4S) By similarityBy similarity
Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S) By similarityBy similarity
Metal bindingi100 – 1001Iron-sulfur 1 (4Fe-4S) By similarityBy similarity

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase beta chain (EC:1.7.99.4)
Alternative name(s):
Respiratory membrane-bound nitrate reductase subunit beta
Gene namesi
Name:narH
OrganismiBradyrhizobium sp.
Taxonomic identifieri376 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Subcellular locationi

Cell membrane. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›266›266Respiratory nitrate reductase beta chainPRO_0000283779Add
BLAST

Expressioni

Inductioni

Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite.1 Publication

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP85098.
SMRiP85098. Positions 1-256.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 32304Fe-4S ferredoxin-type 1Add
BLAST
Domaini30 – 61324Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Contains at least 2 4Fe-4S ferredoxin-type domains.

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEiPS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

P85098-1 [UniParc]FASTAAdd to Basket

« Hide

SQVGMVLNLD KCIGCHTCSV TCKEGMEYAW FNNVESKLCE HCLNPACVAT    50
CPSGAIYKRE EDGIVLIDQD KLCISGCPYK CIFCYPRIES GQPTVCSETC 100
VGRYLGVLLY DADRIEEAAS TEHETDLLYE RQLDVFLDPN DPKVIEQAIK 150
QGIPQNVIDA AQRSPVYKLA LPLHPEYRAA DAGELGSNGI LPDVESLRML 200
SAGDTGPVIR SQTVEGVTDT RALEEVGLTE AQAQEMYRYL AIANYEDRFV 250
VPSSHRIDAI NITEVR 266
Length:266
Mass (Da):29,401
Last modified:April 3, 2007 - v1
Checksum:i67C8E2EA3B0B8D6E
GO

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi11 – 122
Non-adjacent residuesi23 – 242
Non-adjacent residuesi37 – 382
Non-adjacent residuesi58 – 592
Non-adjacent residuesi71 – 722
Non-adjacent residuesi80 – 812
Non-adjacent residuesi87 – 882
Non-adjacent residuesi103 – 1042
Non-adjacent residuesi114 – 1152
Non-adjacent residuesi131 – 1322
Non-adjacent residuesi143 – 1442
Non-adjacent residuesi150 – 1512
Non-adjacent residuesi163 – 1642
Non-adjacent residuesi168 – 1692
Non-adjacent residuesi178 – 1792
Non-adjacent residuesi198 – 1992
Non-adjacent residuesi210 – 2112
Non-adjacent residuesi221 – 2222
Non-adjacent residuesi238 – 2392
Non-adjacent residuesi248 – 2492
Non-adjacent residuesi256 – 2572

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei266 – 2661

Cross-referencesi

3D structure databases

ProteinModelPortali P85098.
SMRi P85098. Positions 1-256.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR017896. 4Fe4S_Fe-S-bd.
[Graphical view ]
Pfami PF13247. Fer4_11. 1 hit.
[Graphical view ]
PROSITEi PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
    Polcyn W.
    Submitted (FEB-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
    Strain: USDA 3045.

Entry informationi

Entry nameiNARH_BRASZ
AccessioniPrimary (citable) accession number: P85098
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3