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P85098

- NARH_BRASZ

UniProt

P85098 - NARH_BRASZ

Protein

Respiratory nitrate reductase beta chain

Gene

narH

Organism
Bradyrhizobium sp.
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.By similarity1 Publication

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.1 Publication

    Cofactori

    Binds 3 4Fe-4S clusters per subunit.By similarity
    Binds 1 3Fe-4S cluster per subunit.By similarity

    Enzyme regulationi

    Inhibited by micromolar concentrations of azide.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi18 – 181Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi22 – 221Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi39 – 391Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi42 – 421Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi47 – 471Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi51 – 511Iron-sulfur 4 (3Fe-4S)By similarity
    Metal bindingi73 – 731Iron-sulfur 4 (3Fe-4S)By similarity
    Metal bindingi77 – 771Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi84 – 841Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi100 – 1001Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory nitrate reductase beta chain (EC:1.7.99.4)
    Alternative name(s):
    Respiratory membrane-bound nitrate reductase subunit beta
    Gene namesi
    Name:narH
    OrganismiBradyrhizobium sp.
    Taxonomic identifieri376 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

    Subcellular locationi

    Cell membrane 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›266›266Respiratory nitrate reductase beta chainPRO_0000283779Add
    BLAST

    Expressioni

    Inductioni

    Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite.1 Publication

    Interactioni

    Subunit structurei

    Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP85098.
    SMRiP85098. Positions 1-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 32304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini30 – 61324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains at least 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    [Graphical view]
    PfamiPF13247. Fer4_11. 1 hit.
    [Graphical view]
    PROSITEiPS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P85098-1 [UniParc]FASTAAdd to Basket

    « Hide

    SQVGMVLNLD KCIGCHTCSV TCKEGMEYAW FNNVESKLCE HCLNPACVAT    50
    CPSGAIYKRE EDGIVLIDQD KLCISGCPYK CIFCYPRIES GQPTVCSETC 100
    VGRYLGVLLY DADRIEEAAS TEHETDLLYE RQLDVFLDPN DPKVIEQAIK 150
    QGIPQNVIDA AQRSPVYKLA LPLHPEYRAA DAGELGSNGI LPDVESLRML 200
    SAGDTGPVIR SQTVEGVTDT RALEEVGLTE AQAQEMYRYL AIANYEDRFV 250
    VPSSHRIDAI NITEVR 266
    Length:266
    Mass (Da):29,401
    Last modified:April 3, 2007 - v1
    Checksum:i67C8E2EA3B0B8D6E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi11 – 1221 Publication
    Non-adjacent residuesi23 – 2421 Publication
    Non-adjacent residuesi37 – 3821 Publication
    Non-adjacent residuesi58 – 5921 Publication
    Non-adjacent residuesi71 – 7221 Publication
    Non-adjacent residuesi80 – 8121 Publication
    Non-adjacent residuesi87 – 8821 Publication
    Non-adjacent residuesi103 – 10421 Publication
    Non-adjacent residuesi114 – 11521 Publication
    Non-adjacent residuesi131 – 13221 Publication
    Non-adjacent residuesi143 – 14421 Publication
    Non-adjacent residuesi150 – 15121 Publication
    Non-adjacent residuesi163 – 16421 Publication
    Non-adjacent residuesi168 – 16921 Publication
    Non-adjacent residuesi178 – 17921 Publication
    Non-adjacent residuesi198 – 19921 Publication
    Non-adjacent residuesi210 – 21121 Publication
    Non-adjacent residuesi221 – 22221 Publication
    Non-adjacent residuesi238 – 23921 Publication
    Non-adjacent residuesi248 – 24921 Publication
    Non-adjacent residuesi256 – 25721 Publication
    Non-terminal residuei266 – 26611 Publication

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P85098.
    SMRi P85098. Positions 1-256.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR017896. 4Fe4S_Fe-S-bd.
    [Graphical view ]
    Pfami PF13247. Fer4_11. 1 hit.
    [Graphical view ]
    PROSITEi PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
      Polcyn W.
      Submitted (FEB-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
      Strain: USDA 30451 Publication.

    Entry informationi

    Entry nameiNARH_BRASZ
    AccessioniPrimary (citable) accession number: P85098
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3