P85098 (NARH_BRASZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Respiratory nitrate reductase beta chain
EC=1.7.99.4

Alternative name(s):
Respiratory membrane-bound nitrate reductase subunit beta

Gene names

Name:

narH

Organism

Bradyrhizobium sp.

Taxonomic identifier

376 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	266 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit. Ref.1 UniProtKB P11349
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. Ref.1
Cofactor	Binds 3 4Fe-4S clusters per subunit By similarity. UniProtKB P11349 Binds 1 3Fe-4S cluster per subunit By similarity. UniProtKB P11349
Enzyme regulation	Inhibited by micromolar concentrations of azide. Ref.1
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane. Ref.1
Subcellular location	Cell membrane. Cytoplasm Ref.1.
Induction	Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite. Ref.1
Sequence similarities	Contains at least 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Repeat
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›266	›266	Respiratory nitrate reductase beta chain		PRO_0000283779
Regions
	Domain	3 – 32	30	4Fe-4S ferredoxin-type 1
	Domain	30 – 61	32	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	12	1	Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	15	1	Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	18	1	Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	22	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	39	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	42	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	47	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	51	1	Iron-sulfur 4 (3Fe-4S) By similarity UniProtKB P11349
	Metal binding	73	1	Iron-sulfur 4 (3Fe-4S) By similarity UniProtKB P11349
	Metal binding	77	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	81	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	84	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	96	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
	Metal binding	100	1	Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
Experimental info
	Non-adjacent residues	11 – 12	2
	Non-adjacent residues	23 – 24	2
	Non-adjacent residues	37 – 38	2
	Non-adjacent residues	58 – 59	2
	Non-adjacent residues	71 – 72	2
	Non-adjacent residues	80 – 81	2
	Non-adjacent residues	87 – 88	2
	Non-adjacent residues	103 – 104	2
	Non-adjacent residues	114 – 115	2
	Non-adjacent residues	131 – 132	2
	Non-adjacent residues	143 – 144	2
	Non-adjacent residues	150 – 151	2
	Non-adjacent residues	163 – 164	2
	Non-adjacent residues	168 – 169	2
	Non-adjacent residues	178 – 179	2
	Non-adjacent residues	198 – 199	2
	Non-adjacent residues	210 – 211	2
	Non-adjacent residues	221 – 222	2
	Non-adjacent residues	238 – 239	2
	Non-adjacent residues	248 – 249	2
	Non-adjacent residues	256 – 257	2
	Non-terminal residue	266	1

Sequences

	Sequence		Length	Mass (Da)
	P85098 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 67C8E2EA3B0B8D6E	FASTA	266	29,401
	10 20 30 40 50 60 SQVGMVLNLD KCIGCHTCSV TCKEGMEYAW FNNVESKLCE HCLNPACVAT CPSGAIYKRE 70 80 90 100 110 120 EDGIVLIDQD KLCISGCPYK CIFCYPRIES GQPTVCSETC VGRYLGVLLY DADRIEEAAS 130 140 150 160 170 180 TEHETDLLYE RQLDVFLDPN DPKVIEQAIK QGIPQNVIDA AQRSPVYKLA LPLHPEYRAA 190 200 210 220 230 240 DAGELGSNGI LPDVESLRML SAGDTGPVIR SQTVEGVTDT RALEEVGLTE AQAQEMYRYL 250 260 AIANYEDRFV VPSSHRIDAI NITEVR

References

[1]

"Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry." Polcyn W. Submitted (FEB-2007) to UniProtKB Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION. Strain: USDA 3045.

Cross-references

3D structure databases
ProteinModelPortal	P85098.
SMR	P85098. Positions 1-256.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. [Graphical view]
Pfam	PF13247. Fer4_11. 1 hit. [Graphical view]
PROSITE	PS00198. 4FE4S_FER_1. False negative. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARH_BRASZ

Accession

Primary (citable) accession number: P85098

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	November 28, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program