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P85098 (NARH_BRASZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Respiratory nitrate reductase beta chain

EC=1.7.99.4
Alternative name(s):
Respiratory membrane-bound nitrate reductase subunit beta
Gene names
Name:narH
OrganismBradyrhizobium sp.
Taxonomic identifier376 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length266 AA.
Sequence statusFragments.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit. Ref.1 UniProtKB P11349

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. Ref.1

Cofactor

Binds 3 4Fe-4S clusters per subunit By similarity. UniProtKB P11349

Binds 1 3Fe-4S cluster per subunit By similarity. UniProtKB P11349

Enzyme regulation

Inhibited by micromolar concentrations of azide. Ref.1

Subunit structure

Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane. Ref.1

Subcellular location

Cell membrane. Cytoplasm Ref.1.

Induction

Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite. Ref.1

Sequence similarities

Contains at least 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›266›266Respiratory nitrate reductase beta chain
PRO_0000283779

Regions

Domain3 – 32304Fe-4S ferredoxin-type 1
Domain30 – 61324Fe-4S ferredoxin-type 2

Sites

Metal binding121Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
Metal binding151Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
Metal binding181Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349
Metal binding221Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
Metal binding391Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
Metal binding421Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
Metal binding471Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
Metal binding511Iron-sulfur 4 (3Fe-4S) By similarity UniProtKB P11349
Metal binding731Iron-sulfur 4 (3Fe-4S) By similarity UniProtKB P11349
Metal binding771Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB P11349
Metal binding811Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
Metal binding841Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
Metal binding961Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB P11349
Metal binding1001Iron-sulfur 1 (4Fe-4S) By similarity UniProtKB P11349

Experimental info

Non-adjacent residues11 – 122
Non-adjacent residues23 – 242
Non-adjacent residues37 – 382
Non-adjacent residues58 – 592
Non-adjacent residues71 – 722
Non-adjacent residues80 – 812
Non-adjacent residues87 – 882
Non-adjacent residues103 – 1042
Non-adjacent residues114 – 1152
Non-adjacent residues131 – 1322
Non-adjacent residues143 – 1442
Non-adjacent residues150 – 1512
Non-adjacent residues163 – 1642
Non-adjacent residues168 – 1692
Non-adjacent residues178 – 1792
Non-adjacent residues198 – 1992
Non-adjacent residues210 – 2112
Non-adjacent residues221 – 2222
Non-adjacent residues238 – 2392
Non-adjacent residues248 – 2492
Non-adjacent residues256 – 2572
Non-terminal residue2661

Sequences

Sequence LengthMass (Da)Tools
P85098 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 67C8E2EA3B0B8D6E

FASTA26629,401
        10         20         30         40         50         60 
SQVGMVLNLD KCIGCHTCSV TCKEGMEYAW FNNVESKLCE HCLNPACVAT CPSGAIYKRE 

        70         80         90        100        110        120 
EDGIVLIDQD KLCISGCPYK CIFCYPRIES GQPTVCSETC VGRYLGVLLY DADRIEEAAS 

       130        140        150        160        170        180 
TEHETDLLYE RQLDVFLDPN DPKVIEQAIK QGIPQNVIDA AQRSPVYKLA LPLHPEYRAA 

       190        200        210        220        230        240 
DAGELGSNGI LPDVESLRML SAGDTGPVIR SQTVEGVTDT RALEEVGLTE AQAQEMYRYL 

       250        260 
AIANYEDRFV VPSSHRIDAI NITEVR 

« Hide

References

[1]"Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
Polcyn W.
Submitted (FEB-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
Strain: USDA 3045.

Cross-references

3D structure databases

ProteinModelPortalP85098.
SMRP85098. Positions 1-256.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR017896. 4Fe4S_Fe-S-bd.
[Graphical view]
PfamPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEPS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARH_BRASZ
AccessionPrimary (citable) accession number: P85098
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families