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P85097

- NARG_BRASZ

UniProt

P85097 - NARG_BRASZ

Protein

Respiratory nitrate reductase alpha chain

Gene

narG

Organism
Bradyrhizobium sp.
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction.By similarity1 Publication

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.1 Publication

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Enzyme regulationi

    Inhibited by micromolar concentrations of azide.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi29 – 291Iron-sulfur (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi33 – 331Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi37 – 371Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. molybdenum ion binding Source: InterPro
    4. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory nitrate reductase alpha chain (EC:1.7.99.4)
    Alternative name(s):
    Respiratory membrane-bound nitrate reductase subunit alpha
    Gene namesi
    Name:narG
    OrganismiBradyrhizobium sp.
    Taxonomic identifieri376 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

    Subcellular locationi

    Cell membrane 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›902›902Respiratory nitrate reductase alpha chainPRO_0000283778Add
    BLAST

    Expressioni

    Inductioni

    Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite.1 Publication

    Interactioni

    Subunit structurei

    Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP85097.
    SMRiP85097. Positions 2-900.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR028189. Nitr_red_alph_N.
    [Graphical view]
    PfamiPF00384. Molybdopterin. 2 hits.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P85097-1 [UniParc]FASTAAdd to Basket

    « Hide

    FSPIDRYNDN HTQETYEDRE WENVYRSTHG VNCTGSCSWK DGIVTWEGQE    50
    LNYPTTGPDM PDFEPRGASY SWYLYSANRG VLWSMWQEEL QNNESPLDAW 100
    KSSWQEVNEL IAASNVYTVK TYGPDRVAGF SPIPAMSMVS YASGARTPDA 150
    HFFTEVRVVS VSPDFAESTK LCDLWLAPKQ GTDAAMALAM GHVMLREFHL 200
    DNPSDYFLNY CRQYTDFPFL VTLSKQKGDQ FVADRAADLV DALGQENNPE 250
    WKPVLWNENT NDFATPHGTM GSRGKWNLEQ RLEDEETGEK LSVLGIEDEI 300
    GTVRLQLADG STALVTTVYD LTMANYGLER GLGGQEPKDF NDDVPFTPAW 350
    QEKITGVPRE LIIQIAREFA DNADKTHGRA VLNLVLLVGA QGVNGGGWAH 400
    YVGQEKLRPA EGWQTIAMAK HMNSTSYFYN HSSQWRYETV TAQELLSPMA 450
    DKYQHHGDYN VLAARMGWLP SAPQLGTNPL RQAGMSPVDY TVKFAAEQPE 500
    NGKNHPRNLF VWRSNLLGSS GKGHEYMLKY LLGTENGIQG KQGGVKPEEV 550
    EWKLSSTCLY SDIVLPTATW YEKDDMNTSD MHPFIHPLSA AVDPAWESKS 600
    DWDIFTSLSK KFSEVCVGHL GKETDVVTLP IQHDSAAELA QPLDVKDWKG 650
    ECEPIPGKTM PQIHVVERDY PATYERFTSI GPLMEKGIAW NTQSEMDLLR 700
    AWAALSEFTG RDHTHLALNK EDEKFRDIQA QPRTVITSPA FTGSEKQSFY 750
    LDHDMMKDFG ESLLVYRPPI DTRKSRPEVE GKEITLNYLT PHNKGGPIVW 800
    ISETDARDLG IEDNDWIEVF NSNGALTARV PAGMTMMYHA QERGGIHNSV 850
    TRPTHMIGGY AQLAYGFNYY GTVGSNRDEF VVVRNINWLD GEGNDQVQES 900
    VK 902
    Length:902
    Mass (Da):101,519
    Last modified:April 3, 2007 - v1
    Checksum:i93540162AA633C66
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi6 – 721 Publication
    Non-adjacent residuesi26 – 2721 Publication
    Non-adjacent residuesi40 – 4121 Publication
    Non-adjacent residuesi66 – 6721 Publication
    Non-adjacent residuesi79 – 8021 Publication
    Non-adjacent residuesi101 – 10221 Publication
    Non-adjacent residuesi120 – 12121 Publication
    Non-adjacent residuesi126 – 12721 Publication
    Non-adjacent residuesi146 – 14721 Publication
    Non-adjacent residuesi157 – 15821 Publication
    Non-adjacent residuesi170 – 17121 Publication
    Non-adjacent residuesi179 – 18021 Publication
    Non-adjacent residuesi196 – 19721 Publication
    Non-adjacent residuesi212 – 21321 Publication
    Non-adjacent residuesi225 – 22621 Publication
    Non-adjacent residuesi235 – 23621 Publication
    Non-adjacent residuesi252 – 25321 Publication
    Non-adjacent residuesi273 – 27421 Publication
    Non-adjacent residuesi281 – 28221 Publication
    Non-adjacent residuesi290 – 29121 Publication
    Non-adjacent residuesi304 – 30521 Publication
    Non-adjacent residuesi330 – 33121 Publication
    Non-adjacent residuesi338 – 33921 Publication
    Non-adjacent residuesi353 – 35421 Publication
    Non-adjacent residuesi359 – 36021 Publication
    Non-adjacent residuesi367 – 36821 Publication
    Non-adjacent residuesi379 – 38021 Publication
    Non-adjacent residuesi406 – 40721 Publication
    Non-adjacent residuesi420 – 42121 Publication
    Non-adjacent residuesi436 – 43721 Publication
    Non-adjacent residuesi452 – 45321 Publication
    Non-adjacent residuesi465 – 46621 Publication
    Non-adjacent residuesi481 – 48221 Publication
    Non-adjacent residuesi493 – 49421 Publication
    Non-adjacent residuesi507 – 50821 Publication
    Non-adjacent residuesi513 – 51421 Publication
    Non-adjacent residuesi522 – 52321 Publication
    Non-adjacent residuesi529 – 53021 Publication
    Non-adjacent residuesi541 – 54221 Publication
    Non-adjacent residuesi553 – 55421 Publication
    Non-adjacent residuesi573 – 57421 Publication
    Non-adjacent residuesi599 – 60021 Publication
    Non-adjacent residuesi610 – 61121 Publication
    Non-adjacent residuesi622 – 62321 Publication
    Non-adjacent residuesi649 – 65021 Publication
    Non-adjacent residuesi658 – 65921 Publication
    Non-adjacent residuesi668 – 66921 Publication
    Non-adjacent residuesi676 – 67721 Publication
    Non-adjacent residuesi686 – 68721 Publication
    Non-adjacent residuesi700 – 70121 Publication
    Non-adjacent residuesi711 – 71221 Publication
    Non-adjacent residuesi724 – 72521 Publication
    Non-adjacent residuesi733 – 73421 Publication
    Non-adjacent residuesi746 – 74721 Publication
    Non-adjacent residuesi757 – 75821 Publication
    Non-adjacent residuesi773 – 77421 Publication
    Non-adjacent residuesi782 – 78321 Publication
    Non-adjacent residuesi794 – 79521 Publication
    Non-adjacent residuesi807 – 80821 Publication
    Non-adjacent residuesi829 – 83021 Publication
    Non-adjacent residuesi843 – 84421 Publication
    Non-adjacent residuesi852 – 85321 Publication
    Non-adjacent residuesi884 – 88521 Publication
    Non-terminal residuei902 – 90211 Publication

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P85097.
    SMRi P85097. Positions 2-900.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR028189. Nitr_red_alph_N.
    [Graphical view ]
    Pfami PF00384. Molybdopterin. 2 hits.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    PROSITEi PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
      Polcyn W.
      Submitted (FEB-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
      Strain: USDA 30451 Publication.

    Entry informationi

    Entry nameiNARG_BRASZ
    AccessioniPrimary (citable) accession number: P85097
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3