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P85097 (NARG_BRASZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Respiratory nitrate reductase alpha chain

EC=1.7.99.4
Alternative name(s):
Respiratory membrane-bound nitrate reductase subunit alpha
Gene names
Name:narG
OrganismBradyrhizobium sp.
Taxonomic identifier376 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length902 AA.
Sequence statusFragments.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction. Ref.1 UniProtKB P09152

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. Ref.1

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. UniProtKB P09152

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Enzyme regulation

Inhibited by micromolar concentrations of azide. Ref.1

Subunit structure

Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane. Ref.1

Subcellular location

Cell membrane. Cytoplasm Ref.1.

Induction

Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite. Ref.1

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›902›902Respiratory nitrate reductase alpha chain
PRO_0000283778

Sites

Metal binding291Iron-sulfur (4Fe-4S); via pros nitrogen By similarity UniProtKB P09152
Metal binding331Iron-sulfur (4Fe-4S) By similarity UniProtKB P09152
Metal binding371Iron-sulfur (4Fe-4S) By similarity UniProtKB P09152

Experimental info

Non-adjacent residues6 – 72
Non-adjacent residues26 – 272
Non-adjacent residues40 – 412
Non-adjacent residues66 – 672
Non-adjacent residues79 – 802
Non-adjacent residues101 – 1022
Non-adjacent residues120 – 1212
Non-adjacent residues126 – 1272
Non-adjacent residues146 – 1472
Non-adjacent residues157 – 1582
Non-adjacent residues170 – 1712
Non-adjacent residues179 – 1802
Non-adjacent residues196 – 1972
Non-adjacent residues212 – 2132
Non-adjacent residues225 – 2262
Non-adjacent residues235 – 2362
Non-adjacent residues252 – 2532
Non-adjacent residues273 – 2742
Non-adjacent residues281 – 2822
Non-adjacent residues290 – 2912
Non-adjacent residues304 – 3052
Non-adjacent residues330 – 3312
Non-adjacent residues338 – 3392
Non-adjacent residues353 – 3542
Non-adjacent residues359 – 3602
Non-adjacent residues367 – 3682
Non-adjacent residues379 – 3802
Non-adjacent residues406 – 4072
Non-adjacent residues420 – 4212
Non-adjacent residues436 – 4372
Non-adjacent residues452 – 4532
Non-adjacent residues465 – 4662
Non-adjacent residues481 – 4822
Non-adjacent residues493 – 4942
Non-adjacent residues507 – 5082
Non-adjacent residues513 – 5142
Non-adjacent residues522 – 5232
Non-adjacent residues529 – 5302
Non-adjacent residues541 – 5422
Non-adjacent residues553 – 5542
Non-adjacent residues573 – 5742
Non-adjacent residues599 – 6002
Non-adjacent residues610 – 6112
Non-adjacent residues622 – 6232
Non-adjacent residues649 – 6502
Non-adjacent residues658 – 6592
Non-adjacent residues668 – 6692
Non-adjacent residues676 – 6772
Non-adjacent residues686 – 6872
Non-adjacent residues700 – 7012
Non-adjacent residues711 – 7122
Non-adjacent residues724 – 7252
Non-adjacent residues733 – 7342
Non-adjacent residues746 – 7472
Non-adjacent residues757 – 7582
Non-adjacent residues773 – 7742
Non-adjacent residues782 – 7832
Non-adjacent residues794 – 7952
Non-adjacent residues807 – 8082
Non-adjacent residues829 – 8302
Non-adjacent residues843 – 8442
Non-adjacent residues852 – 8532
Non-adjacent residues884 – 8852
Non-terminal residue9021

Sequences

Sequence LengthMass (Da)Tools
P85097 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 93540162AA633C66

FASTA902101,519
        10         20         30         40         50         60 
FSPIDRYNDN HTQETYEDRE WENVYRSTHG VNCTGSCSWK DGIVTWEGQE LNYPTTGPDM 

        70         80         90        100        110        120 
PDFEPRGASY SWYLYSANRG VLWSMWQEEL QNNESPLDAW KSSWQEVNEL IAASNVYTVK 

       130        140        150        160        170        180 
TYGPDRVAGF SPIPAMSMVS YASGARTPDA HFFTEVRVVS VSPDFAESTK LCDLWLAPKQ 

       190        200        210        220        230        240 
GTDAAMALAM GHVMLREFHL DNPSDYFLNY CRQYTDFPFL VTLSKQKGDQ FVADRAADLV 

       250        260        270        280        290        300 
DALGQENNPE WKPVLWNENT NDFATPHGTM GSRGKWNLEQ RLEDEETGEK LSVLGIEDEI 

       310        320        330        340        350        360 
GTVRLQLADG STALVTTVYD LTMANYGLER GLGGQEPKDF NDDVPFTPAW QEKITGVPRE 

       370        380        390        400        410        420 
LIIQIAREFA DNADKTHGRA VLNLVLLVGA QGVNGGGWAH YVGQEKLRPA EGWQTIAMAK 

       430        440        450        460        470        480 
HMNSTSYFYN HSSQWRYETV TAQELLSPMA DKYQHHGDYN VLAARMGWLP SAPQLGTNPL 

       490        500        510        520        530        540 
RQAGMSPVDY TVKFAAEQPE NGKNHPRNLF VWRSNLLGSS GKGHEYMLKY LLGTENGIQG 

       550        560        570        580        590        600 
KQGGVKPEEV EWKLSSTCLY SDIVLPTATW YEKDDMNTSD MHPFIHPLSA AVDPAWESKS 

       610        620        630        640        650        660 
DWDIFTSLSK KFSEVCVGHL GKETDVVTLP IQHDSAAELA QPLDVKDWKG ECEPIPGKTM 

       670        680        690        700        710        720 
PQIHVVERDY PATYERFTSI GPLMEKGIAW NTQSEMDLLR AWAALSEFTG RDHTHLALNK 

       730        740        750        760        770        780 
EDEKFRDIQA QPRTVITSPA FTGSEKQSFY LDHDMMKDFG ESLLVYRPPI DTRKSRPEVE 

       790        800        810        820        830        840 
GKEITLNYLT PHNKGGPIVW ISETDARDLG IEDNDWIEVF NSNGALTARV PAGMTMMYHA 

       850        860        870        880        890        900 
QERGGIHNSV TRPTHMIGGY AQLAYGFNYY GTVGSNRDEF VVVRNINWLD GEGNDQVQES 


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References

[1]"Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
Polcyn W.
Submitted (FEB-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
Strain: USDA 3045.

Cross-references

3D structure databases

ProteinModelPortalP85097.
SMRP85097. Positions 2-900.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamPF00384. Molybdopterin. 2 hits.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
PROSITEPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARG_BRASZ
AccessionPrimary (citable) accession number: P85097
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families