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P85097

- NARG_BRASZ

UniProt

P85097 - NARG_BRASZ

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Protein
Respiratory nitrate reductase alpha chain
Gene
narG
Organism
Bradyrhizobium sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The nitrate reductase enzyme complex allows Bradyrhizobium sp. USDA 3045 to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction.By similarity1 Publication

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.1 Publication

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.By similarity
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Enzyme regulationi

Inhibited by micromolar concentrations of azide.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Iron-sulfur (4Fe-4S); via pros nitrogen By similarityBy similarity
Metal bindingi33 – 331Iron-sulfur (4Fe-4S) By similarityBy similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S) By similarityBy similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. molybdenum ion binding Source: InterPro
  4. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase alpha chain (EC:1.7.99.4)
Alternative name(s):
Respiratory membrane-bound nitrate reductase subunit alpha
Gene namesi
Name:narG
OrganismiBradyrhizobium sp.
Taxonomic identifieri376 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Subcellular locationi

Cell membrane. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›902›902Respiratory nitrate reductase alpha chain
PRO_0000283778Add
BLAST

Expressioni

Inductioni

Induced by anaerobiosis, there is no significant expression in an aerobic environment. Expression is further induced in the presence of nitrate or nitrite.1 Publication

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP85097.
SMRiP85097. Positions 2-900.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamiPF00384. Molybdopterin. 2 hits.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

P85097-1 [UniParc]FASTAAdd to Basket

« Hide

FSPIDRYNDN HTQETYEDRE WENVYRSTHG VNCTGSCSWK DGIVTWEGQE    50
LNYPTTGPDM PDFEPRGASY SWYLYSANRG VLWSMWQEEL QNNESPLDAW 100
KSSWQEVNEL IAASNVYTVK TYGPDRVAGF SPIPAMSMVS YASGARTPDA 150
HFFTEVRVVS VSPDFAESTK LCDLWLAPKQ GTDAAMALAM GHVMLREFHL 200
DNPSDYFLNY CRQYTDFPFL VTLSKQKGDQ FVADRAADLV DALGQENNPE 250
WKPVLWNENT NDFATPHGTM GSRGKWNLEQ RLEDEETGEK LSVLGIEDEI 300
GTVRLQLADG STALVTTVYD LTMANYGLER GLGGQEPKDF NDDVPFTPAW 350
QEKITGVPRE LIIQIAREFA DNADKTHGRA VLNLVLLVGA QGVNGGGWAH 400
YVGQEKLRPA EGWQTIAMAK HMNSTSYFYN HSSQWRYETV TAQELLSPMA 450
DKYQHHGDYN VLAARMGWLP SAPQLGTNPL RQAGMSPVDY TVKFAAEQPE 500
NGKNHPRNLF VWRSNLLGSS GKGHEYMLKY LLGTENGIQG KQGGVKPEEV 550
EWKLSSTCLY SDIVLPTATW YEKDDMNTSD MHPFIHPLSA AVDPAWESKS 600
DWDIFTSLSK KFSEVCVGHL GKETDVVTLP IQHDSAAELA QPLDVKDWKG 650
ECEPIPGKTM PQIHVVERDY PATYERFTSI GPLMEKGIAW NTQSEMDLLR 700
AWAALSEFTG RDHTHLALNK EDEKFRDIQA QPRTVITSPA FTGSEKQSFY 750
LDHDMMKDFG ESLLVYRPPI DTRKSRPEVE GKEITLNYLT PHNKGGPIVW 800
ISETDARDLG IEDNDWIEVF NSNGALTARV PAGMTMMYHA QERGGIHNSV 850
TRPTHMIGGY AQLAYGFNYY GTVGSNRDEF VVVRNINWLD GEGNDQVQES 900
VK 902
Length:902
Mass (Da):101,519
Last modified:April 3, 2007 - v1
Checksum:i93540162AA633C66
GO

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi6 – 72
Non-adjacent residuesi26 – 272
Non-adjacent residuesi40 – 412
Non-adjacent residuesi66 – 672
Non-adjacent residuesi79 – 802
Non-adjacent residuesi101 – 1022
Non-adjacent residuesi120 – 1212
Non-adjacent residuesi126 – 1272
Non-adjacent residuesi146 – 1472
Non-adjacent residuesi157 – 1582
Non-adjacent residuesi170 – 1712
Non-adjacent residuesi179 – 1802
Non-adjacent residuesi196 – 1972
Non-adjacent residuesi212 – 2132
Non-adjacent residuesi225 – 2262
Non-adjacent residuesi235 – 2362
Non-adjacent residuesi252 – 2532
Non-adjacent residuesi273 – 2742
Non-adjacent residuesi281 – 2822
Non-adjacent residuesi290 – 2912
Non-adjacent residuesi304 – 3052
Non-adjacent residuesi330 – 3312
Non-adjacent residuesi338 – 3392
Non-adjacent residuesi353 – 3542
Non-adjacent residuesi359 – 3602
Non-adjacent residuesi367 – 3682
Non-adjacent residuesi379 – 3802
Non-adjacent residuesi406 – 4072
Non-adjacent residuesi420 – 4212
Non-adjacent residuesi436 – 4372
Non-adjacent residuesi452 – 4532
Non-adjacent residuesi465 – 4662
Non-adjacent residuesi481 – 4822
Non-adjacent residuesi493 – 4942
Non-adjacent residuesi507 – 5082
Non-adjacent residuesi513 – 5142
Non-adjacent residuesi522 – 5232
Non-adjacent residuesi529 – 5302
Non-adjacent residuesi541 – 5422
Non-adjacent residuesi553 – 5542
Non-adjacent residuesi573 – 5742
Non-adjacent residuesi599 – 6002
Non-adjacent residuesi610 – 6112
Non-adjacent residuesi622 – 6232
Non-adjacent residuesi649 – 6502
Non-adjacent residuesi658 – 6592
Non-adjacent residuesi668 – 6692
Non-adjacent residuesi676 – 6772
Non-adjacent residuesi686 – 6872
Non-adjacent residuesi700 – 7012
Non-adjacent residuesi711 – 7122
Non-adjacent residuesi724 – 7252
Non-adjacent residuesi733 – 7342
Non-adjacent residuesi746 – 7472
Non-adjacent residuesi757 – 7582
Non-adjacent residuesi773 – 7742
Non-adjacent residuesi782 – 7832
Non-adjacent residuesi794 – 7952
Non-adjacent residuesi807 – 8082
Non-adjacent residuesi829 – 8302
Non-adjacent residuesi843 – 8442
Non-adjacent residuesi852 – 8532
Non-adjacent residuesi884 – 8852

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei902 – 9021

Cross-referencesi

3D structure databases

ProteinModelPortali P85097.
SMRi P85097. Positions 2-900.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR028189. Nitr_red_alph_N.
[Graphical view ]
Pfami PF00384. Molybdopterin. 2 hits.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
PROSITEi PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus) USDA 3045 by tandem mass spectrometry."
    Polcyn W.
    Submitted (FEB-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
    Strain: USDA 3045.

Entry informationi

Entry nameiNARG_BRASZ
AccessioniPrimary (citable) accession number: P85097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3