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P85084 (CHIT_CARPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endochitinase
EC=3.2.1.14
OrganismCarica papaya (Papaya)
Taxonomic identifier3649 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Caricaceae › Carica

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Defense against chitin containing fungal pathogens. Shows activity on chitin, tetra-N-acetylglucosamine and chitosan. Ref.1

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Ref.1

Subcellular location

Vacuole By similarity. Note: Vacuolar and protoplast By similarity. UniProtKB P19171

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Mass spectrometry

Molecular mass is 26534 Da from positions 1 - 243. Determined by ESI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Endochitinase
PRO_0000285971

Amino acid modifications

Disulfide bond23 ↔ 85 By similarity UniProtKB Q9FRV1
Disulfide bond97 ↔ 105 By similarity UniProtKB Q9FRV1
Disulfide bond223 ↔ 236 By similarity UniProtKB Q9FRV1

Secondary structure

1 .............................. 243
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P85084 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 51F48F5F9A722703

FASTA24326,541
        10         20         30         40         50         60 
GIEKIISRSM FDQMLKHRNN PACPAKGFYT YDAFLAAAKS FPSFGTTGST DVRKRELAAF 

        70         80         90        100        110        120 
LGQTSHETTG GWPSAPDGPY AWGYCFLKER NPSSNYCAPS PRYPCAPGKS YYGRGPLQLS 

       130        140        150        160        170        180 
WNYNYGPCGE ALRVNLLGNP DLVATDRVLS FKTALWFWMT PQAPKPSCHD VLTGRWQPSA 

       190        200        210        220        230        240 
ADTAAGRLPG YGVLTNLLNG GLECGKGPNP QVADRLGFFR RYCGLLGVGT GNNLDCYNQR 


PFG

« Hide

References

[1]"Structural characterization of two papaya chitinases, a family GH19 of glycosyl hydrolases."
Huet J., Wyckmans J., Wintjens R., Boussard P., Raussens V., Vandenbussche G., Ruysschaert J.M., Azarkan M., Looze Y.
Cell. Mol. Life Sci. 63:3042-3054(2006) [PubMed: 17115118] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY.
Tissue: Latex.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CQLX-ray1.50A/B1-243[»]
ProteinModelPortalP85084.
SMRP85084. Positions 1-243.
ModBaseSearch...

Protein family/group databases

CAZyGH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
SUPFAMSSF53955. SSF53955. 1 hit.
PROSITEPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHIT_CARPA
AccessionPrimary (citable) accession number: P85084
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: May 31, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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