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Protein

Endochitinase

Gene
N/A
Organism
Carica papaya (Papaya)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Defense against chitin-containing fungal pathogens. Shows activity on chitin, tetra-N-acetylglucosamine and chitosan.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BRENDAi3.2.1.14. 1191.

Protein family/group databases

CAZyiGH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase (EC:3.2.1.14)
OrganismiCarica papaya (Papaya)
Taxonomic identifieri3649 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCaricaceaeCarica

Subcellular locationi

  • Vacuole By similarity

  • Note: Vacuolar and protoplast.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002859711 – 243EndochitinaseAdd BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 85By similarity
Disulfide bondi97 ↔ 105By similarity
Disulfide bondi223 ↔ 236By similarity

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Helixi8 – 14Combined sources7
Turni15 – 19Combined sources5
Turni24 – 28Combined sources5
Helixi31 – 39Combined sources9
Turni42 – 45Combined sources4
Helixi50 – 67Combined sources18
Helixi79 – 81Combined sources3
Beta strandi101 – 103Combined sources3
Turni115 – 118Combined sources4
Helixi122 – 132Combined sources11
Turni136 – 138Combined sources3
Helixi140 – 144Combined sources5
Helixi147 – 159Combined sources13
Helixi168 – 172Combined sources5
Helixi180 – 185Combined sources6
Helixi191 – 203Combined sources13
Beta strandi204 – 207Combined sources4
Helixi210 – 226Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CQLX-ray1.50A/B1-243[»]
ProteinModelPortaliP85084.
SMRiP85084.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP85084.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
InterProiIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P85084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GIEKIISRSM FDQMLKHRNN PACPAKGFYT YDAFLAAAKS FPSFGTTGST
60 70 80 90 100
DVRKRELAAF LGQTSHETTG GWPSAPDGPY AWGYCFLKER NPSSNYCAPS
110 120 130 140 150
PRYPCAPGKS YYGRGPLQLS WNYNYGPCGE ALRVNLLGNP DLVATDRVLS
160 170 180 190 200
FKTALWFWMT PQAPKPSCHD VLTGRWQPSA ADTAAGRLPG YGVLTNLLNG
210 220 230 240
GLECGKGPNP QVADRLGFFR RYCGLLGVGT GNNLDCYNQR PFG
Length:243
Mass (Da):26,541
Last modified:May 1, 2007 - v1
Checksum:i51F48F5F9A722703
GO

Mass spectrometryi

Molecular mass is 26534 Da from positions 1 - 243. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CQLX-ray1.50A/B1-243[»]
ProteinModelPortaliP85084.
SMRiP85084.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.14. 1191.

Miscellaneous databases

EvolutionaryTraceiP85084.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
InterProiIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIT_CARPA
AccessioniPrimary (citable) accession number: P85084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.