Reviewed, UniProtKB/Swiss-Prot P85084 (CHIT_CARPA)

Names and origin

Protein names	Recommended name:     Endochitinase     EC=3.2.1.14
Organism	Carica papaya (Papaya)
Taxonomic identifier	3649 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Caricaceae › Carica

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens. Shows activity on chitin, tetra-N-acetylglucosamine and chitosan. Ref.1
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Ref.1
Subcellular location	Vacuole By similarity. Note: Vacuolar and protoplast By similarity. UniProtKB P19171
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.
Mass spectrometry	Molecular mass is 26534 Da from positions 1 - 243. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Vacuole
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Endochitinase		PRO_0000285971
Amino acid modifications
	Disulfide bond	23 ↔ 85		By similarity UniProtKB Q9FRV1
	Disulfide bond	97 ↔ 105		By similarity UniProtKB Q9FRV1
	Disulfide bond	223 ↔ 236		By similarity UniProtKB Q9FRV1

Sequences

	Sequence		Length	Mass (Da)
	P85084-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 51F48F5F9A722703	FASTA	243	26,541
	10 20 30 40 50 60 GIEKIISRSM FDQMLKHRNN PACPAKGFYT YDAFLAAAKS FPSFGTTGST DVRKRELAAF 70 80 90 100 110 120 LGQTSHETTG GWPSAPDGPY AWGYCFLKER NPSSNYCAPS PRYPCAPGKS YYGRGPLQLS 130 140 150 160 170 180 WNYNYGPCGE ALRVNLLGNP DLVATDRVLS FKTALWFWMT PQAPKPSCHD VLTGRWQPSA 190 200 210 220 230 240 ADTAAGRLPG YGVLTNLLNG GLECGKGPNP QVADRLGFFR RYCGLLGVGT GNNLDCYNQR PFG

References

[1]	"Structural characterization of two papaya chitinases, a family GH19 of glycosyl hydrolases." Huet J., Wyckmans J., Wintjens R., Boussard P., Raussens V., Vandenbussche G., Ruysschaert J.M., Azarkan M., Looze Y. Cell. Mol. Life Sci. 63:3042-3054(2006) [PubMed: 17115118] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Latex.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CQL	X-ray	1.50	A/B	1-243	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH19. Glycoside Hydrolase Family 19.

Enzyme and pathway databases

BRENDA

3.2.1.14. 18730.

Family and domain databases

InterPro

IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
[Graphical view]

PANTHER

PTHR22595. Glyco_hydro_19_cat. 1 hit.

Pfam

PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]

PIRSF

PIRSF001060. Endochitinase. 1 hit.

ProDom

PD354900. Glyco_hydro_19. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CHIT_CARPA

Accession

Primary (citable) accession number: P85084

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)