Reviewed,
UniProtKB/Swiss-Prot P85084 (CHIT_CARPA)
Last modified
June 16, 2009.
Version 17.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Endochitinase EC=3.2.1.14 |
| Organism | Carica papaya (Papaya) |
| Taxonomic identifier | 3649 [NCBI] |
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Caricaceae › Carica |
Protein attributes
| Sequence length | 243 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Defense against chitin containing fungal pathogens. Shows activity on chitin, tetra-N-acetylglucosamine and chitosan. Ref.1 |
| Catalytic activity | Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Ref.1 |
| Subcellular location | Vacuole By similarity. Note: Vacuolar and protoplast By similarity. UniProtKB P19171 |
| Sequence similarities | Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. |
| Mass spectrometry | Molecular mass is 26534 Da from positions 1 - 243. Determined by ESI. Ref.1 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation |
| Cellular component | Vacuole |
| Ligand | Chitin-binding |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Technical term | 3D-structure Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic processInferred from electronic annotation. Source: UniProtKB-KW defense responseInferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 243 | 243 | Endochitinase | PRO_0000285971 | |||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 23 ↔ 85 | By similarity UniProtKB Q9FRV1 | |||||||||
| Disulfide bond | 97 ↔ 105 | By similarity UniProtKB Q9FRV1 | |||||||||
| Disulfide bond | 223 ↔ 236 | By similarity UniProtKB Q9FRV1 | |||||||||
Sequences
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References
| [1] | "Structural characterization of two papaya chitinases, a family GH19 of glycosyl hydrolases." Huet J., Wyckmans J., Wintjens R., Boussard P., Raussens V., Vandenbussche G., Ruysschaert J.M., Azarkan M., Looze Y. Cell. Mol. Life Sci. 63:3042-3054(2006) [PubMed: 17115118] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Latex. |
| + | Additional computationally mapped references. |
Cross-references
3D structure databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| ModBase | Search... | ||||||||||||
Protein family/group databases | |||||||||||||
| CAZy | GH19. Glycoside Hydrolase Family 19. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 3.2.1.14. 18730. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view] | ||||||||||||
| PANTHER | PTHR22595. Glyco_hydro_19_cat. 1 hit. | ||||||||||||
| Pfam | PF00182. Glyco_hydro_19. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF001060. Endochitinase. 1 hit. | ||||||||||||
| ProDom | PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| PROSITE | PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | CHIT_CARPA | ||||||||
| Accession | Primary (citable) accession number: P85084 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


