Reviewed, UniProtKB/Swiss-Prot P85080 (GTF1_LEUME)

Names and origin

Protein names	Recommended name:     Glucosyltransferase 1     EC=2.4.1.5 Alternative name(s):     Dextransucrase     Sucrose 6-glucosyltransferase     Glucansucrase
Organism	Leuconostoc mesenteroides
Taxonomic identifier	1245 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	6 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Catalytic activity	Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1). Ref.1
Miscellaneous	Synthesizes water-soluble glucans (alpha 1,6-glucose). Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 70 family.
Biophysicochemical properties	Kinetic parameters: KM=69.88 mM for sucrose Ref.1 pH dependence: Optimum pH is 5.0. Ref.1 Temperature dependence: Optimum temperature is 35 degrees Celsius. Ref.1

Ontologies

Keywords
Disease	Dental caries
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	dextransucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›6	›6	Glucosyltransferase 1		PRO_0000284553
Experimental info
	Non-terminal residue	6	1

Sequences

	Sequence		Length	Mass (Da)
	P85080-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 72D1A451B5BAC000	FASTA	6	636
	DSTNTV

References

[1]	"Purification and characterization of glucansucrase from Leuconostoc mesentriodes AA1." Aman A., Ul-qader S.A., Azhar A., Syed M.N. Submitted (JAN-2007) to UniProtKB Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AA1.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.4.1.5. 703.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

GTF1_LEUME

Accession

Primary (citable) accession number: P85080

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	January 20, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)