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Protein

Pectinesterase

Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Pectin + n H2O = n methanol + pectate.1 Publication

Enzyme regulationi

Inhibited by PMEI.1 Publication

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1
Sitei136Transition state stabilizerBy similarity1
Active sitei137Proton donorPROSITE-ProRule annotation1
Active sitei158NucleophilePROSITE-ProRule annotation1
Binding sitei226SubstrateBy similarity1
Binding sitei228SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi3.1.1.11. 121.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase (EC:3.1.1.11)
Short name:
PE
Alternative name(s):
Pectin methylesterase
OrganismiActinidia deliciosa (Kiwi)
Taxonomic identifieri3627 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei3547. Act d 7.
3976. Act d 7.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003117231 – 321PectinesteraseAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylthreonine1 Publication1
Glycosylationi75N-linked (GlcNAc...) (complex)1 Publication1
Disulfide bondi151 ↔ 171By similarity
Glycosylationi275N-linked (GlcNAc...) (complex)1 Publication1
Glycosylationi290N-linked (GlcNAc...) (complex)1 Publication1
Glycosylationi319N-linked (GlcNAc...) (complex)1 Publication1

Post-translational modificationi

The N-glycans attached at Asn-75, Asn-275, Asn-290 and Asn-319 are complex oligosaccharides containing xylose, fucose, hexose and N-acetylglucosamine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei19Not glycosylated1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP85076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Sequence analysis

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P85076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TAVTDIVPDV VVAKDGSGNF TTVGAAVAAA KDSSTARFVI YIKEGAYFEY
60 70 80 90 100
VDVDKKKTNL MFIGDGIGKT WIKGNRSVVD GWTTFRSSTV AVVGTGFIAR
110 120 130 140 150
GISFENYAGP SKHQAVALRS GADFSAFYQC SFVGYQDTLY VHSLRQFYSE
160 170 180 190 200
CDVYGTIDFI FGNAAAVLQK CNLYARKPNE NQKNIFTAQG RDDPNQNTGI
210 220 230 240 250
SILNCKVAAA ADLIPVLSSF KTYLGRPWKE YSRTVFLLSQ MESLIDPAGW
260 270 280 290 300
LEWSGDFALT TLYYREYKNT GPGSNTTARV TWPGYAVTTN ETEVIQFTVG
310 320
NFIQGSQWLT SYNIPVYLNL T
Length:321
Mass (Da):35,368
Last modified:November 13, 2007 - v1
Checksum:i26CEC95A8071C04D
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP85076.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3547. Act d 7.
3976. Act d 7.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BRENDAi3.1.1.11. 121.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME_ACTDE
AccessioniPrimary (citable) accession number: P85076
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: October 5, 2016
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.