Reviewed, UniProtKB/Swiss-Prot P85076 (PME_ACTDE)

Names and origin

Protein names	Recommended name:     Pectinesterase       Short name=PE     EC=3.1.1.11 Alternative name(s):     Pectin methylesterase
Organism	Actinidia deliciosa (Kiwi)
Taxonomic identifier	3627 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Actinidiaceae › Actinidia

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Pectin + n H2O = n methanol + pectate. Ref.1
Enzyme regulation	Inhibited by PMEI. Ref.1
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Post-translational modification	The N-glycans attached at Asn-75, Asn-275, Asn-290 and Asn-319 are complex oligosaccharides containing xylose, fucose, hexose and N-acetylglucosamine. Ref.1
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Molecular function	Aspartyl esterase Hydrolase
PTM	Acetylation Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Pectinesterase		PRO_0000311723
Sites
	Active site	137	1	Proton donor By similarity UniProtKB P0C1A8
	Active site	158	1	Nucleophile By similarity UniProtKB P0C1A8
	Binding site	84	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity
	Site	19	1	Not glycosylated
	Site	136	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylthreonine Ref.1
	Glycosylation	75	1	N-linked (GlcNAc...) (complex) Ref.1
	Glycosylation	275	1	N-linked (GlcNAc...) (complex) Ref.1
	Glycosylation	290	1	N-linked (GlcNAc...) (complex) Ref.1
	Glycosylation	319	1	N-linked (GlcNAc...) (complex) Ref.1
	Disulfide bond	151 ↔ 171		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P85076-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 26CEC95A8071C04D	FASTA	321	35,368
	10 20 30 40 50 60 TAVTDIVPDV VVAKDGSGNF TTVGAAVAAA KDSSTARFVI YIKEGAYFEY VDVDKKKTNL 70 80 90 100 110 120 MFIGDGIGKT WIKGNRSVVD GWTTFRSSTV AVVGTGFIAR GISFENYAGP SKHQAVALRS 130 140 150 160 170 180 GADFSAFYQC SFVGYQDTLY VHSLRQFYSE CDVYGTIDFI FGNAAAVLQK CNLYARKPNE 190 200 210 220 230 240 NQKNIFTAQG RDDPNQNTGI SILNCKVAAA ADLIPVLSSF KTYLGRPWKE YSRTVFLLSQ 250 260 270 280 290 300 MESLIDPAGW LEWSGDFALT TLYYREYKNT GPGSNTTARV TWPGYAVTTN ETEVIQFTVG 310 320 NFIQGSQWLT SYNIPVYLNL T

References

[1]

"The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor." Ciardiello M.A., D'Avino R., Amoresano A., Tuppo L., Carpentieri A., Carratore V., Tamburrini M., Giovane A., Pucci P., Camardella L. Proteins 71:195-206(2007) [PubMed: 17932919] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ENZYME REGULATION, GLYCOSYLATION AT ASN-75; ASN-275; ASN-290 AND ASN-319, ACETYLATION AT THR-1. Tissue: Fruit.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.11. 267086.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME_ACTDE

Accession

Primary (citable) accession number: P85076

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	October 13, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)