P85064 (SPI_SCHGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Greglin
Organism	Schistocerca gregaria (Desert locust)
Taxonomic identifier	7010 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Orthopteroidea › Orthoptera › Caelifera › Acridomorpha › Acridoidea › Acrididae › Cyrtacanthacridinae › Schistocerca

Protein attributes

Sequence length	83 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine protease inhibitor. Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin. Ref.1
Biophysicochemical properties	pH dependence: No decrease in activity was observed after incubating at pH 2.5 and at pH 11.0 overnight. Ref.1 Temperature dependence: Thermostable. No decrease in activity was observed after heating for 1 hour at 90 degrees Celsius. Ref.1

Ontologies

Keywords
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 83

83

Greglin

PRO_0000271408

Amino acid modifications

Modified residue

8

1

Phosphoserine Ref.1

Modified residue

11

1

Phosphoserine Ref.1

Modified residue

15

1

Phosphoserine Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

83

Helix Strand Turn

Details...

Beta strand

24 – 26

3

Beta strand

32 – 36

5

Beta strand

41 – 46

6

Helix

47 – 57

11

Beta strand

61 – 63

3

Helix

66 – 68

3

Sequences

	Sequence		Length	Mass (Da)
	P85064 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 0004698C2DF1B158	FASTA	83	9,188
	10 20 30 40 50 60 SEDDGSVSPE SQEMSYLELP LPSISPLIYA PVCVEDSNSD FYLFVNECEV RKCGCEAGFV 70 80 YTFVPREMCK ATTSLCPMQT KSS

References

[1]

"A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase." Brillard-Bourdet M., Hamdaoui A., Hajjar E., Boudier C., Reuter N., Ehret-Sabatier L., Bieth J.G., Gauthier F. Biochem. J. 400:467-476(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION AT SER-8; SER-11 AND SER-15. Tissue: Ovary.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GI3	X-ray	1.75	C	1-83	[»]

ModBase

Search...

Protein family/group databases

MEROPS

I01.059.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

SPI_SCHGR

Accession

Primary (citable) accession number: P85064

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	June 16, 2009
Last modified:	April 3, 2013
This is version 11 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)