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P85064 (SPI_SCHGR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length83 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor. Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin. Ref.1

Biophysicochemical properties

pH dependence:

No decrease in activity was observed after incubating at pH 2.5 and at pH 11.0 overnight. Ref.1

Temperature dependence:

Thermostable. No decrease in activity was observed after heating for 1 hour at 90 degrees Celsius. Ref.1

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8383Greglin
PRO_0000271408

Amino acid modifications

Modified residue81Phosphoserine Ref.1
Modified residue111Phosphoserine Ref.1
Modified residue151Phosphoserine Ref.1

Secondary structure

............ 83
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P85064 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 0004698C2DF1B158

FASTA839,188
        10         20         30         40         50         60 
SEDDGSVSPE SQEMSYLELP LPSISPLIYA PVCVEDSNSD FYLFVNECEV RKCGCEAGFV 

        70         80 
YTFVPREMCK ATTSLCPMQT KSS 

« Hide

References

[1]"A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase."
Brillard-Bourdet M., Hamdaoui A., Hajjar E., Boudier C., Reuter N., Ehret-Sabatier L., Bieth J.G., Gauthier F.
Biochem. J. 400:467-476(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION AT SER-8; SER-11 AND SER-15.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GI3X-ray1.75C1-83[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP85064. 1 interaction.
MINTMINT-8397571.

Protein family/group databases

MEROPSI01.059.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameSPI_SCHGR
AccessionPrimary (citable) accession number: P85064
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 15 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references