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Protein

Greglin

Gene
N/A
Organism
Schistocerca gregaria (Desert locust)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor (PubMed:16839309, PubMed:23075397). Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin (PubMed:16839309).2 Publications

pH dependencei

No decrease in activity observed after incubating at pH 2.5, pH 7.4 and at pH 11.0 for 1 hour to overnight (PubMed:16839309, PubMed:23075397).2 Publications

Temperature dependencei

Thermostable (PubMed:16839309, PubMed:23075397). No decrease in activity was observed after heating for 1 hour at up to 95 degrees Celsius (PubMed:16839309, PubMed:23075397).2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.059.

Names & Taxonomyi

Protein namesi
Recommended name:
Greglin
OrganismiSchistocerca gregaria (Desert locust)
Taxonomic identifieri7010 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaOrthopteraCaeliferaAcridomorphaAcridoideaAcrididaeCyrtacanthacridinaeSchistocerca

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8383GreglinPRO_0000271408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphoserine2 Publications
Modified residuei11 – 111Phosphoserine1 Publication
Modified residuei15 – 151Phosphoserine1 Publication
Disulfide bondi21 ↔ 551 Publication
Disulfide bondi25 ↔ 481 Publication
Disulfide bondi33 ↔ 691 Publication
Disulfide bondi53 ↔ 761 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Protein-protein interaction databases

IntActiP85064. 1 interaction.
MINTiMINT-8397571.

Structurei

Secondary structure

1
83
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 263Combined sources
Beta strandi32 – 365Combined sources
Beta strandi41 – 466Combined sources
Helixi47 – 5711Combined sources
Beta strandi61 – 633Combined sources
Helixi66 – 683Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GI3X-ray1.75C1-83[»]
ModBaseiSearch...
MobiDBiSearch...

Sequencei

Sequence statusi: Complete.

P85064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SEDDGSASPE SQEMSYTELP CPSICPLIYA PVCVEDSNQD FYLFVNECEV
60 70 80
RKCGCEAGFV YTFVPREMCK ATTSLCPMQT KSS
Length:83
Mass (Da):9,195
Last modified:April 29, 2015 - v3
Checksum:iCFEAA69ABDCC39EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71A → V AA sequence (PubMed:16839309).Curated
Sequence conflicti17 – 259TELPCPSIC → LELPLPSIS AA sequence (PubMed:16839309).Curated
Sequence conflicti39 – 391Q → S AA sequence (PubMed:16839309).Curated

Mass spectrometryi

Molecular mass is 9661.13 Da from positions 1 - 83. Determined by ESI. 1 Publication
Molecular mass is 9733.08 Da from positions 1 - 83. Determined by ESI. Mono-phosphorylated form.1 Publication
Molecular mass is 9813.06 Da from positions 1 - 83. Determined by ESI. Di-phosphorylated form.1 Publication
Molecular mass is 9893.04 Da from positions 1 - 83. Determined by ESI. Tri-phosphorylated form.1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GI3X-ray1.75C1-83[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP85064. 1 interaction.
MINTiMINT-8397571.

Protein family/group databases

MEROPSiI01.059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase."
    Brillard-Bourdet M., Hamdaoui A., Hajjar E., Boudier C., Reuter N., Ehret-Sabatier L., Bieth J.G., Gauthier F.
    Biochem. J. 400:467-476(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION AT SER-8; SER-11 AND SER-15.
    Tissue: Ovary1 Publication.
  2. "Crystal structure of greglin, a novel non-classical Kazal inhibitor, in complex with subtilisin."
    Derache C., Epinette C., Roussel A., Gabant G., Cadene M., Korkmaz B., Gauthier F., Kellenberger C.
    FEBS J. 279:4466-4478(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, DISULFIDE BONDS, PHOSPHORYLATION AT SER-8, X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 21-78 IN COMPLEX WITH SUBTILISIN.
    Tissue: Ovary1 Publication.

Entry informationi

Entry nameiSPI_SCHGR
AccessioniPrimary (citable) accession number: P85064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: April 29, 2015
Last modified: April 29, 2015
This is version 18 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.