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P85046 (PRP1_SIMDA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phenoloxidase subunit 1
EC=1.14.18.1
Alternative name(s):
PO-P1
OrganismSimulium damnosum (Black fly)
Taxonomic identifier37338 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Chironomoidea › Simuliidae › Simulium ›

Protein attributes

Sequence length195 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.

Catalytic activity

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.

L-tyrosine + O2 = dopaquinone + H2O.

Cofactor

Binds 2 copper ions per subunit By similarity. UniProtKB Q27451

Subunit structure

Heterodimer By similarity. UniProtKB Q27451

Subcellular location

Secreted By similarity.

Induction

By infection with O.dukei and O.ochengi. Ref.1

Sequence similarities

Belongs to the tyrosinase family.

Ontologies

Keywords
   Biological processMelanin biosynthesis
   Cellular componentSecreted
   LigandCopper
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processmelanin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

monophenol monooxygenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›195›195Phenoloxidase subunit 1
PRO_0000271231

Sites

Metal binding101Copper B By similarity UniProtKB Q27451

Amino acid modifications

Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential

Experimental info

Non-terminal residue11
Non-terminal residue1951

Sequences

Sequence LengthMass (Da)Tools
P85046 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 137ECB8E37454C4F

FASTA19522,867
        10         20         30         40         50         60 
MRDPFFYRWH SYIDDIFQEH KERLRPYTEA QLNFNGITVT GVQVAPERGP TNTFQTSWQQ 

        70         80         90        100        110        120 
SDVDLSRGMD FVAPRGNVTA RFTHLNHTPF TYSIQVNNSS GAQRMGMVRI FLAPKTDERG 

       130        140        150        160        170        180 
NEMLFRDQRL MMIEMDKFVV SMRPGQNTIR RRSTESTVTI PFERTFRSLE ESRPDQTTDA 

       190 
QQQFNFCGCG WPHHM

« Hide

References

[1]"Simulium damnosum s.l.: isolation and identification of prophenoloxidase following an infection with Onchocerca spp. using targeted differential display."
Hagen H.-E., Klager S.L., McKerrow J.H., Ham P.J.
Exp. Parasitol. 86:213-218(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.

Cross-references

3D structure databases

ProteinModelPortalP85046.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
2.60.40.1520. 1 hit.
InterProIPR013788. Hemocyanin/hexamerin.
IPR005203. Hemocyanin_C.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERPTHR11511. PTHR11511. 1 hit.
PfamPF03723. Hemocyanin_C. 1 hit.
[Graphical view]
SUPFAMSSF48056. Di-copper_centre. 1 hit.
SSF81296. Ig_E-set. 1 hit.
PROSITEPS00209. HEMOCYANIN_1. Partial match.
PS00210. HEMOCYANIN_2. Partial match.
PS00497. TYROSINASE_1. Partial match.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRP1_SIMDA
AccessionPrimary (citable) accession number: P85046
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: March 6, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

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