Reviewed, UniProtKB/Swiss-Prot P85046 (PRP1_SIMDA)

Names and origin

Protein names	Recommended name:     Phenoloxidase subunit 1     EC=1.14.18.1 Alternative name(s):     PO-P1
Organism	Simulium damnosum (Black fly)
Taxonomic identifier	37338 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Chironomoidea › Simuliidae › Simulium

Protein attributes

Sequence length	195 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone.
Catalytic activity	L-tyrosine + L-dopa + O2 = L-dopa + dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit By similarity. UniProtKB Q27451
Subunit structure	Heterodimer By similarity. UniProtKB Q27451
Subcellular location	Secreted By similarity.
Induction	By infection with O.dukei and O.ochengi. Ref.1
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Cellular component	Secreted
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	melanin biosynthetic process from tyrosine Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC oxygen transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›195	›195	Phenoloxidase subunit 1		PRO_0000271231
Sites
	Metal binding	10	1	Copper B By similarity UniProtKB Q27451
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	195	1

Sequences

	Sequence		Length	Mass (Da)
	P85046-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 137ECB8E37454C4F	FASTA	195	22,867
	10 20 30 40 50 60 MRDPFFYRWH SYIDDIFQEH KERLRPYTEA QLNFNGITVT GVQVAPERGP TNTFQTSWQQ 70 80 90 100 110 120 SDVDLSRGMD FVAPRGNVTA RFTHLNHTPF TYSIQVNNSS GAQRMGMVRI FLAPKTDERG 130 140 150 160 170 180 NEMLFRDQRL MMIEMDKFVV SMRPGQNTIR RRSTESTVTI PFERTFRSLE ESRPDQTTDA 190 QQQFNFCGCG WPHHM

References

[1]

"Simulium damnosum s.l.: isolation and identification of prophenoloxidase following an infection with Onchocerca spp. using targeted differential display." Hagen H.-E., Klager S.L., McKerrow J.H., Ham P.J. Exp. Parasitol. 86:213-218(1997) [PubMed: 9225772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.18.1. 293908.
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR013788. Hemocyanin. IPR005203. Hemocyanin_C. IPR000896. Hemocyanin_Cu. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit. G3DSA:2.60.40.1520. hemocyanin_C. 1 hit.
PANTHER	PTHR11511. Hemocyanin. 1 hit.
Pfam	PF03723. Hemocyanin_C. 1 hit. PF00372. Hemocyanin_M. 1 hit. [Graphical view]
PROSITE	PS00209. HEMOCYANIN_1. Partial match. PS00210. HEMOCYANIN_2. Partial match. PS00497. TYROSINASE_1. Partial match. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRP1_SIMDA

Accession

Primary (citable) accession number: P85046

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)