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P85045

- LYS_BUFGA

UniProt

P85045 - LYS_BUFGA

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Protein
Lysozyme C
Gene
N/A
Organism
Bufo gargarizans andrewsi (Andrew's toad) (Bufo andrewsi)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has antibacterial activity against the Gram-positive bacterium S.aureus and against the Gram-negative bacterium E.coli with a MIC of 1 µM and 8 µM respectively. No antifungal activity against C.albicans.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Stable from 20 degrees Celsius to 70 degrees Celsius. Activity decreases sharply above 70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511 By similarityBy similarity
Active sitei69 – 691 By similarityBy similarity

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. cytolysis Source: UniProtKB-KW
  3. defense response to Gram-negative bacterium Source: UniProtKB
  4. defense response to Gram-positive bacterium Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme C (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase C
OrganismiBufo gargarizans andrewsi (Andrew's toad) (Bufo andrewsi)
Taxonomic identifieri61428 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaHyloideaBufonidaeBufo

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 16›161 Publication
Add
BLAST
Chaini17 – 146130Lysozyme C1 Publication
PRO_0000271190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 144 By similarityBy similarity
Disulfide bondi46 ↔ 132 By similarityBy similarity
Disulfide bondi81 ↔ 97 By similarityBy similarity
Disulfide bondi93 ↔ 111 By similarityBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP85045.

Expressioni

Tissue specificityi

Expressed by the skin glands.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP85045.
SMRiP85045. Positions 19-146.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052297.

Family and domain databases

InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00137. LYSOZYME.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P85045-1 [UniParc]FASTAAdd to Basket

« Hide

SGKYISWEDS CSYLQLQKYE RCELAKALKK GGLADFKGYS LENWICTAFH    50
ESGYNTASTN YNPPDKSTDY GIFQINSRWW CNDYKTPRSK NTCNIDCKVL 100
LGDDISPAIK CAKRVVSDPN GMGAWVAWKK YCKGKNLSQW TQGCKL 146
Length:146
Mass (Da):16,608
Last modified:January 9, 2007 - v1
Checksum:i126AA86ED3D01EBB
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Cross-referencesi

3D structure databases

ProteinModelPortali P85045.
SMRi P85045. Positions 19-146.
ModBasei Search...

Proteomic databases

PRIDEi P85045.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG052297.

Family and domain databases

InterProi IPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00062. Lys. 1 hit.
[Graphical view ]
PRINTSi PR00137. LYSOZYME.
PR00135. LYZLACT.
SMARTi SM00263. LYZ1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
PROSITEi PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification of a lysozyme from skin secretions of Bufo andrewsi."
    Zhao Y., Jin Y., Lee W.-H., Zhang Y.
    Comp. Biochem. Physiol. 142C:46-52(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-33, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Skin secretion.

Entry informationi

Entry nameiLYS_BUFGA
AccessioniPrimary (citable) accession number: P85045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: February 6, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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