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Protein

Forkhead box protein K1

Gene

FOXK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Important regulatory factor of the myogenic progenitor cell population. Involved in the cell cycle process, promotes proliferation by repressing Foxo4 transcriptional activity and the cyclin-dependent kinase inhibitor, p21CIP, in the myogenic progenitor cells. Represses myogenic differentiation by inhibiting MEFC acitivity. Has a role in remodeling processes of adult muscles that occur in response to physiological stimuli. Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed:25805136).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi305 – 400Fork-headPROSITE-ProRule annotationAdd BLAST96

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-32104-MONOMER.
ReactomeiR-HSA-5689603. UCH proteinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein K1
Alternative name(s):
Myocyte nuclear factor
Short name:
MNF
Gene namesi
Name:FOXK12 Publications
Synonyms:MNF1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:23480. FOXK1.

Subcellular locationi

  • Nucleus Sequence analysis1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi355H → A: No effect on interaction with DVL2. 1 Publication1

Organism-specific databases

DisGeNETi221937.
OpenTargetsiENSG00000164916.
PharmGKBiPA134978307.

Polymorphism and mutation databases

BioMutaiFOXK1.
DMDMi118572324.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002616672 – 733Forkhead box protein K1Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei161Omega-N-methylarginineCombined sources1
Modified residuei191Omega-N-methylarginineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1
Modified residuei239PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1
Modified residuei245PhosphothreonineBy similarity1
Modified residuei247PhosphothreonineBy similarity1
Modified residuei253PhosphoserineCombined sources1
Modified residuei257PhosphoserineCombined sources1
Modified residuei295PhosphoserineBy similarity1
Modified residuei299PhosphoserineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei422PhosphothreonineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei436PhosphothreonineCombined sources1
Modified residuei441PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei459PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP85037.
MaxQBiP85037.
PaxDbiP85037.
PeptideAtlasiP85037.
PRIDEiP85037.

PTM databases

iPTMnetiP85037.
PhosphoSitePlusiP85037.

Expressioni

Tissue specificityi

Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.1 Publication

Gene expression databases

BgeeiENSG00000164916.
CleanExiHS_FOXK1.
ExpressionAtlasiP85037. baseline and differential.
GenevisibleiP85037. HS.

Organism-specific databases

HPAiHPA017998.
HPA018864.

Interactioni

Subunit structurei

Interacts with SIN3A and SIN3B (via PAH2) to form a complex which represses transcription (By similarity). Interacts with FOXO4 and MEF2C; both interactions inhibit FOXO4 and MEF2C transactivation activity (By similarity). Interacts with DVL2 and DVL3; the interaction induces DVL2 nuclear translocation (PubMed:25805136).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FOXO3O435242EBI-2509974,EBI-1644164
IRF2P143162EBI-2509974,EBI-2866589

Protein-protein interaction databases

BioGridi128769. 93 interactors.
IntActiP85037. 63 interactors.
STRINGi9606.ENSP00000328720.

Structurei

3D structure databases

ProteinModelPortaliP85037.
SMRiP85037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 175FHAPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 40Interaction with SIN3A and SIN3BBy similarityAdd BLAST40
Regioni95 – 420Required for interaction with FOXO4 and MEF2CBy similarityAdd BLAST326

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 96Ala-richSequence analysisAdd BLAST87
Compositional biasi35 – 62Pro-richSequence analysisAdd BLAST28

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00800000124014.
HOGENOMiHOG000072588.
HOVERGENiHBG051649.
InParanoidiP85037.
KOiK09404.
OMAiVTIGQHH.
OrthoDBiEOG091G099F.
PhylomeDBiP85037.
TreeFamiTF325718.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR001766. Fork_head_dom.
IPR008984. SMAD_FHA_domain.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: P85037-1) [UniParc]FASTAAdd to basket
Also known as: a1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPAAAPP PAPAQPQPPP
60 70 80 90 100
GPPPPPPPPL PPGAIAGAGS SGGSSGVSGD SAVAGAAPAL VAAAAASVRQ
110 120 130 140 150
SPGPALARLE GREFEFLMRQ PSVTIGRNSS QGSVDLSMGL SSFISRRHLQ
160 170 180 190 200
LSFQEPHFYL RCLGKNGVFV DGAFQRRGAP ALQLPKQCTF RFPSTAIKIQ
210 220 230 240 250
FTSLYHKEEA PASPLRPLYP QISPLKIHIP EPDLRSMVSP VPSPTGTISV
260 270 280 290 300
PNSCPASPRG AGSSSYRFVQ NVTSDLQLAA EFAAKAASEQ QADTSGGDSP
310 320 330 340 350
KDESKPPFSY AQLIVQAISS AQDRQLTLSG IYAHITKHYP YYRTADKGWQ
360 370 380 390 400
NSIRHNLSLN RYFIKVPRSQ EEPGKGSFWR IDPASEAKLV EQAFRKRRQR
410 420 430 440 450
GVSCFRTPFG PLSSRSAPAS PTHPGLMSPR SGGLQTPECL SREGSPIPHD
460 470 480 490 500
PEFGSKLASV PEYRYSQSAP GSPVSAQPVI MAVPPRPSSL VAKPVAYMPA
510 520 530 540 550
SIVTSQQPAG HAIHVVQQAP TVTMVRVVTT SANSANGYIL TSQGAAGGSH
560 570 580 590 600
DAAGAAVLDL GSEARGLEEK PTIAFATIPA AGGVIQTVAS QMAPGVPGHT
610 620 630 640 650
VTILQPATPV TLGQHHLPVR AVTQNGKHAV PTNSLAGNAY ALTSPLQLLA
660 670 680 690 700
TQASSSAPVV VTRVCEVGPK EPAAAVAATA TTTPATATTA SASASSTGEP
710 720 730
EVKRSRVEEP SGAVTTPAGV IAAAGPQGPG TGE
Note: No experimental confirmation available.Curated
Length:733
Mass (Da):75,457
Last modified:November 28, 2006 - v1
Checksum:iC9EF4AC3C959A1C3
GO
Isoform 21 Publication (identifier: P85037-2) [UniParc]FASTAAdd to basket
Also known as: b1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.
     164-187: GKNGVFVDGAFQRRGAPALQLPKQ → MAYCLGVNFVPSRFCYQLHRLLLR

Note: No experimental confirmation available.Curated
Show »
Length:570
Mass (Da):59,702
Checksum:iFF1010BC65780B22
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144I → V in CB959941 (PubMed:15489334).Curated1
Sequence conflicti154 – 155QE → KS in CB959941 (PubMed:15489334).Curated2
Sequence conflicti159Y → S in CB959941 (PubMed:15489334).Curated1
Sequence conflicti217P → A in AW206906 (Ref. 4) Curated1
Sequence conflicti232P → T in AW206906 (Ref. 4) Curated1
Sequence conflicti437 – 445Missing in AK122663 (PubMed:14702039).Curated9

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0522391 – 163Missing in isoform 2. 2 PublicationsAdd BLAST163
Alternative sequenceiVSP_052240164 – 187GKNGV…QLPKQ → MAYCLGVNFVPSRFCYQLHR LLLR in isoform 2. 2 PublicationsAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122663 mRNA. No translation available.
AC072054 Genomic DNA. No translation available.
AC092428 Genomic DNA. No translation available.
AC092610 Genomic DNA. No translation available.
BC038434 mRNA. No translation available.
CB959941 mRNA. No translation available.
AW206906 mRNA. No translation available.
CCDSiCCDS34591.1. [P85037-1]
RefSeqiNP_001032242.1. NM_001037165.1. [P85037-1]
XP_011513493.1. XM_011515191.2. [P85037-2]
UniGeneiHs.487393.

Genome annotation databases

EnsembliENST00000328914; ENSP00000328720; ENSG00000164916. [P85037-1]
GeneIDi221937.
KEGGihsa:221937.
UCSCiuc003snc.2. human. [P85037-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122663 mRNA. No translation available.
AC072054 Genomic DNA. No translation available.
AC092428 Genomic DNA. No translation available.
AC092610 Genomic DNA. No translation available.
BC038434 mRNA. No translation available.
CB959941 mRNA. No translation available.
AW206906 mRNA. No translation available.
CCDSiCCDS34591.1. [P85037-1]
RefSeqiNP_001032242.1. NM_001037165.1. [P85037-1]
XP_011513493.1. XM_011515191.2. [P85037-2]
UniGeneiHs.487393.

3D structure databases

ProteinModelPortaliP85037.
SMRiP85037.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128769. 93 interactors.
IntActiP85037. 63 interactors.
STRINGi9606.ENSP00000328720.

PTM databases

iPTMnetiP85037.
PhosphoSitePlusiP85037.

Polymorphism and mutation databases

BioMutaiFOXK1.
DMDMi118572324.

Proteomic databases

EPDiP85037.
MaxQBiP85037.
PaxDbiP85037.
PeptideAtlasiP85037.
PRIDEiP85037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328914; ENSP00000328720; ENSG00000164916. [P85037-1]
GeneIDi221937.
KEGGihsa:221937.
UCSCiuc003snc.2. human. [P85037-1]

Organism-specific databases

CTDi221937.
DisGeNETi221937.
GeneCardsiFOXK1.
HGNCiHGNC:23480. FOXK1.
HPAiHPA017998.
HPA018864.
MIMi616302. gene.
neXtProtiNX_P85037.
OpenTargetsiENSG00000164916.
PharmGKBiPA134978307.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00800000124014.
HOGENOMiHOG000072588.
HOVERGENiHBG051649.
InParanoidiP85037.
KOiK09404.
OMAiVTIGQHH.
OrthoDBiEOG091G099F.
PhylomeDBiP85037.
TreeFamiTF325718.

Enzyme and pathway databases

BioCyciZFISH:G66-32104-MONOMER.
ReactomeiR-HSA-5689603. UCH proteinases.

Miscellaneous databases

ChiTaRSiFOXK1. human.
GeneWikiiFOXK1.
GenomeRNAii221937.
PROiP85037.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164916.
CleanExiHS_FOXK1.
ExpressionAtlasiP85037. baseline and differential.
GenevisibleiP85037. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR001766. Fork_head_dom.
IPR008984. SMAD_FHA_domain.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOXK1_HUMAN
AccessioniPrimary (citable) accession number: P85037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.