P85001 (CE290_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centrosomal protein of 290 kDa
Short name=Cep290

Gene names

Name:	cep290
ORF Names:	im:7145703

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	2439 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Part of the tectonic complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition. Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes By similarity. Involved in development of the nervous system and kidney. Ref.2
Subunit structure	Part of the tectonic complex By similarity.
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › cilium basal body By similarity. Note: Localizes at the transition zone, a region between the basal body and the ciliary axoneme By similarity.
Developmental stage	At 24 hours post-fertilization (hpf), expressed in the tail in a rostral-to-caudal gradient and more weakly expressed in the cerebellum and retina. At 48 hpf, strongly expressed at the boundary between the developing cerebellum and tectum and in the retina. Ref.2
Miscellaneous	Morpholino knockdown of the protein causes defects in retinal, cerebellar and otic cavity development and cyst formation in pronephric kidney tubules. Ref.2

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation Protein transport Transport
Cellular component	Cell projection Cytoplasm Cytoskeleton Nucleus
Domain	Coiled coil
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cerebellum development Inferred from mutant phenotype Ref.2. Source: ZFIN cilium morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB eye photoreceptor cell development Inferred from mutant phenotype Ref.2. Source: HGNC otic vesicle formation Inferred from mutant phenotype Ref.2. Source: HGNC pronephros development Inferred from mutant phenotype Ref.2. Source: HGNC protein transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	centrosome Inferred from sequence or structural similarity Ref.2. Source: UniProtKB nucleus Inferred from sequence or structural similarity Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2439	2439	Centrosomal protein of 290 kDa		PRO_0000258014
Regions
	Coiled coil	75 – 913	839	Potential
	Coiled coil	1271 – 1576	306	Potential
	Coiled coil	2046 – 2394	349	Potential

Sequences

	Sequence		Length	Mass (Da)
	P85001 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: B64BB271A5C64C71	FASTA	2,439	283,434
	10 20 30 40 50 60 MPAAADWRLL MGMDPEDLGD EDEKICDLIL MVKPRDLKAD DSEKMIQLFR ISQTLLRMKL 70 80 90 100 110 120 DEIKCAYEVV DSAGAEQARI ENELKAKVLK LESELEMAQR VMGGGDKHFL RDEIRQLESH 130 140 150 160 170 180 LERKEKEVTQ LEKEMGKERK SNEELALRAE EAEEKNRKLK REIKQLTRKN EQLQQDIEFY 190 200 210 220 230 240 RKEAEQRESL QTKEESNEIQ RRLTKANQQL YQCMEELQHA EDMAANLRSE NEHLQKNLEE 250 260 270 280 290 300 SVKEMEKMTD EYNKMKIAVQ QTDAIMDQLR KDRDHAKLQV RELTDQIQAR VEEDDPVMAA 310 320 330 340 350 360 VNAKVEEWKS VLSGKDLEIL EYQQMIRDLR EKLRTAQMDS DKSNIIALQQ AVQERDNQIK 370 380 390 400 410 420 MLSEQVEQYT TEMERNAMLI EELKRPLKKD KGHSSDHQRR LEDLSAKLQV AERKVLEAQR 430 440 450 460 470 480 AAQLAERDAR DKDKELNDTL SRIRLYESGT DGLEAAISEI KECKNQIRVR DREIEGMIKE 490 500 510 520 530 540 INQLEMKINN LLDENEDLRE RLGLNPKEEL DLSEFRRSKI LKQRQYKAEN QVLLKEIERL 550 560 570 580 590 600 EEERLELKQR IRALVKDKGV TVVSNSLLDN SVEEKPVRSL RPSSGSTDDE IKRKNERLQK 610 620 630 640 650 660 ELSNKEKELE LRRSESAQFK AKLNEMLNEN KQLEQGMKEI LQAIQDTQKK TPTSTGVSIP 670 680 690 700 710 720 SLERLVNALE MKYSEGKFDA SLHLRTQVDQ LTGRNEELRL EMKTAREEAA NTLSQLTKAN 730 740 750 760 770 780 EKIARLESEM ESMSKSTGSS IPHKTLALPE EMTPTSAEAI NALNEYTVQL LQEIKNKGDS 790 800 810 820 830 840 IEQLGSALEE YKRKFAVIRH QQGLLYKEHQ SERESWQKER DSFAELKSKL EEQREVDAVK 850 860 870 880 890 900 IKEYNHLLET LEKDPSEIRR EMAETGRKIV VLRVNEKCLT RRYTTLLELE QHLRKENAKL 910 920 930 940 950 960 KEDFTQMQAV VTERIGYLQR FKEMAAFKMA SLQKSLDVSV PASELERANK QYTELTIKYR 970 980 990 1000 1010 1020 NLLQKDNHLV QKTTSLEHLE TENMSLRERI DSINKELEIS KEKLHTLEQA FENISTTGGE 1030 1040 1050 1060 1070 1080 IIMDKATKAV ANSEIVSVSR RITTLEMKEL NERQRAEHAQ KMYEHLRNSL KQVEERNFEL 1090 1100 1110 1120 1130 1140 ETKFAELTKL NLEAQRIERE LRDELADSVS KHISDADRKR ITELEKTEAN LRIEVSKLRE 1150 1160 1170 1180 1190 1200 VSDVAKMQVS ALDARQQSRE KEVESLRRQV LDYQAESDEK ALIAKLHQHI VALQLSETTA 1210 1220 1230 1240 1250 1260 ISRLEATNTR LQKLEAQKLR DEQKLDEQQQ ALWHARQEGH QRARHLRHTI QALRRQFSGA 1270 1280 1290 1300 1310 1320 LPLAQQEKFS NTMLHLQEDR ARVREDAQIA EEERRKAEGK AQELELKLKG LEELIATLKD 1330 1340 1350 1360 1370 1380 AKGAQKVSEW HKKLEDVRLL EMRQSRELNT QREEIKYLKN CVAEQECTIS GLEEELVQQN 1390 1400 1410 1420 1430 1440 NLLEERQLIW DQREVQLERQ LDSYEKQQNE VLNTAQKFEE ATGSLPDPNQ PLANQLDYAL 1450 1460 1470 1480 1490 1500 GKIKEHVRTI LETKTTCKIL EEKLKEKEAA LWSSEQNVLS RDKVINELRL RLPAAAEREK 1510 1520 1530 1540 1550 1560 LLADLSKQED SESQPTLKVA HQTINNLQGR LDQKEEVLKK YQNLLGKARQ EQEEIAKRHE 1570 1580 1590 1600 1610 1620 EEVRALHQKL DVYMDTSLDR FKQTALELIK KPTITVPTSK HLVRLAEMEQ TVAEQDNSLS 1630 1640 1650 1660 1670 1680 SLSQKLKIVT QELDQQRQVT AAQAMEHAAD MARLEDKHAA QMKGLSQEAE ELRAQLIQME 1690 1700 1710 1720 1730 1740 KELHYLRTEL EAQKEANVRS PSNTMKNLVE RLKNQLALKE KQLKALSKAL LELRAELTSQ 1750 1760 1770 1780 1790 1800 AEQQIITNAA QKEEALNVQQ IVDKQTKELR ACVRDLNEEL QLAKDGVRAA KARENSLKED 1810 1820 1830 1840 1850 1860 LETLNKDLQR SQKSQNKLQS EKEALEEHLN ELKKKIQRLS SGLQAQVESD GPTVDSLQKK 1870 1880 1890 1900 1910 1920 IRKLEHELDR KSISEPADKR STLKEDKSSK EEVVRWEEGK KWQARVDKMR NVLKEKEREV 1930 1940 1950 1960 1970 1980 DSQAKQLATM KELYSRLEQE KVSLQKKLKG RGVTADQVVG ARTLEADKEI EELHKRNAEL 1990 2000 2010 2020 2030 2040 EQQIKVMKQQ QALPRDAAME DITIRNRYLE ERLYSMESRL SKEPPSRPST SGRGSDTPSQ 2050 2060 2070 2080 2090 2100 REHEFQKENL RLSTENLELR FQLEQANKDL PRLKDQVSDL KEMCSVLKKE KAEVEKRLSH 2110 2120 2130 2140 2150 2160 LRGSGRSGKT IPELEKTIGL MKKVVEKVQR ENENLKKTSE VNVQEQLATL ERDHEKLKSE 2170 2180 2190 2200 2210 2220 YEKLKGKQEE QLNSRLESKT KGIEKIMMEN ERLRKEIKKE AEAAEKLRVA KASLEVANEK 2230 2240 2250 2260 2270 2280 LKAELEETHQ RLLLAQSKGA TLLGVDSKTW KSSVVTRLFE NKMKGLESDI AKKNISISEL 2290 2300 2310 2320 2330 2340 KVQLKEANEK LQATQHTVIQ LKEQVELLKN VPVEATTDEG LAREYQSVRL ANKQLEREKA 2350 2360 2370 2380 2390 2400 QLLRQIQRNE VQLGTNKDGP GYTELQEQIK AANNEKKKLQ DEVRKLTQEL KHFDPTFFEE 2410 2420 2430 LEDLKFNYNL EVKKNIVLEE QLKKLSDQFG VAIPDVSIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	"The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4." Sayer J.A., Otto E.A., O'toole J.F., Nurnberg G., Kennedy M.A., Becker C., Hennies H.C., Helou J., Attanasio M., Fausett B.V., Utsch B., Khanna H., Liu Y., Drummond I., Kawakami I., Kusakabe T., Tsuda M., Ma L. , Lee H., Larson R.G., Allen S.J., Wilkinson C.J., Nigg E.A., Shou C., Lillo C., Williams D.S., Hoppe B., Kemper M.J., Neuhaus T., Parisi M.A., Glass I.A., Petry M., Kispert A., Gloy J., Ganner A., Walz G., Zhu X., Goldman D., Nurnberg P., Swaroop A., Leroux M.R., Hildebrandt F. Nat. Genet. 38:674-681(2006) [PubMed: 16682973] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX901919 Genomic DNA. No translation available.
IPI	IPI00806766.
UniGene	Dr.26022.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P85001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-041111-243. cep290.
Phylogenomic databases
eggNOG	fiNOG13926.
GeneTree	ENSGT00600000084031.
HOGENOM	HBG402900.
HOVERGEN	HBG081077.
InParanoid	P85001.
OrthoDB	EOG4DR9BD.
Gene expression databases
ArrayExpress	P85001.
Bgee	P85001.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

CE290_DANRE

Accession

Primary (citable) accession number: P85001

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program