Reviewed,
UniProtKB/Swiss-Prot P84999 (G3P3_KLUMA)
Last modified
February 9, 2010.
Version 24.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase 3 Short name=GAPDH 3 EC=1.2.1.12 | ||
| Gene names |
| ||
| Organism | Kluyveromyces marxianus (Yeast) (Candida kefyr) | ||
| Taxonomic identifier | 4911 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces |
Protein attributes
| Sequence length | 331 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.1 |
| Pathway | Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Ref.1 |
| Subunit structure | Homotetramer By similarity. UniProtKB P22513 |
| Subcellular location | Cytoplasm By similarity UniProtKB P00359. |
| Sequence similarities | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 331 | 331 | Glyceraldehyde-3-phosphate dehydrogenase 3 | PRO_0000253991 | |||||
Regions | |||||||||
| Nucleotide binding | 11 – 12 | 2 | NAD By similarity UniProtKB P22513 | ||||||
| Region | 148 – 150 | 3 | Glyceraldehyde 3-phosphate binding By similarity UniProtKB P22513 | ||||||
| Region | 208 – 209 | 2 | Glyceraldehyde 3-phosphate binding By similarity UniProtKB P22513 | ||||||
Sites | |||||||||
| Active site | 149 | 1 | Nucleophile By similarity UniProtKB P22513 | ||||||
| Binding site | 33 | 1 | NAD By similarity UniProtKB P22513 | ||||||
| Binding site | 77 | 1 | NAD; via carbonyl oxygen By similarity UniProtKB P22513 | ||||||
| Binding site | 179 | 1 | Glyceraldehyde 3-phosphate By similarity UniProtKB P22513 | ||||||
| Binding site | 231 | 1 | Glyceraldehyde 3-phosphate By similarity UniProtKB P22513 | ||||||
| Binding site | 313 | 1 | NAD By similarity UniProtKB P22513 | ||||||
| Site | 176 | 1 | Activates thiol group during catalysis By similarity UniProtKB P22513 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 148 | 1 | Phosphoserine By similarity UniProtKB P00359 | ||||||
| Modified residue | 177 | 1 | Phosphoserine By similarity UniProtKB P00359 | ||||||
| Modified residue | 200 | 1 | Phosphoserine By similarity UniProtKB P00359 | ||||||
Sequences
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References
| [1] | "Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene family from Kluyveromyces marxianus -- polymerase chain reaction-single-strand conformation polymorphism as a tool for the study of multigenic families." Fernandes P.A., Sena-Esteves M., Moradas-Ferreira P. Yeast 11:725-733(1995) [PubMed: 7668042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PATHWAY. Strain: ATCC 10022 / CBS 6432 / NCTC 2303 / NRRL Y-665. |
Cross-references
Sequence databases | |
|---|---|
| PIR | S57281. |
3D structure databases | |
| SMR | P84999. Positions 1-329. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.2.1.12. 97088. |
Family and domain databases | |
| InterPro | IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020832. GlycerAld_3-P_DH_cat_sub. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR000173. GlycerAld_3-P_DH_subfam. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| PANTHER | PTHR10836. GAP_DH. 1 hit. |
| Pfam | PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000149. GAP_DH. 1 hit. |
| PRINTS | PR00078. G3PDHDRGNASE. |
| SMART | SM00846. Gp_dh_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01534. GAPDH-I. 1 hit. |
| PROSITE | PS00071. GAPDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G3P3_KLUMA | ||||||||
| Accession | Primary (citable) accession number: P84999 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


