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Protein

Stromal interaction molecule 1

Gene

Stim1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores (By similarity). Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit ORAI1 (PubMed:16208375). Involved in enamel formation (By similarity). Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi76 – 8712By similarityAdd
BLAST

GO - Molecular functioni

  • calcium channel regulator activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • microtubule plus-end binding Source: UniProtKB
  • store-operated calcium channel activity Source: RGD

GO - Biological processi

  • activation of store-operated calcium channel activity Source: UniProtKB
  • cellular calcium ion homeostasis Source: GO_Central
  • detection of calcium ion Source: UniProtKB
  • enamel mineralization Source: UniProtKB
  • regulation of calcium ion transport Source: UniProtKB
  • regulation of store-operated calcium entry Source: RGD
  • store-operated calcium entry Source: RGD
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5578775. Ion homeostasis.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 1
Gene namesi
Name:Stim1Imported
Synonyms:SimBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1306831. Stim1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 213191ExtracellularSequence analysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Topological domaini235 – 685451CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: RGD
  • growth cone Source: RGD
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • plasma membrane Source: RGD
  • sarcoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane, Microtubule, Sarcoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi76 – 761D → A: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication
Mutagenesisi78 – 781D → A: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication
Mutagenesisi87 – 871E → Q: Increases Ca(2+) influx through activation of CRAC channels, even when Ca(2+) stores are not depleted. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 685663Stromal interaction molecule 1Sequence analysisPRO_0000248270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence analysis
Modified residuei257 – 2571PhosphoserineCombined sources
Modified residuei504 – 5041PhosphothreonineBy similarity
Modified residuei512 – 5121PhosphoserineCombined sources
Modified residuei517 – 5171PhosphothreonineBy similarity
Modified residuei519 – 5191PhosphoserineCombined sources
Modified residuei521 – 5211PhosphoserineCombined sources
Modified residuei523 – 5231PhosphoserineBy similarity
Modified residuei524 – 5241PhosphoserineBy similarity
Modified residuei567 – 5671PhosphoserineCombined sources
Modified residuei575 – 5751PhosphoserineCombined sources
Modified residuei602 – 6021PhosphoserineBy similarity
Modified residuei608 – 6081PhosphoserineBy similarity
Modified residuei618 – 6181PhosphoserineBy similarity
Modified residuei621 – 6211PhosphoserineBy similarity
Modified residuei628 – 6281PhosphoserineBy similarity
Modified residuei660 – 6601PhosphoserineCombined sources
Modified residuei665 – 6651PhosphothreonineBy similarity
Modified residuei668 – 6681PhosphoserineCombined sources

Post-translational modificationi

Glycosylation is required for cell surface expression.By similarity
Phosphorylated predominantly on Ser residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP84903.
PRIDEiP84903.

PTM databases

iPTMnetiP84903.
PhosphoSiteiP84903.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020425.
ExpressionAtlasiP84903. baseline and differential.
GenevisibleiP84903. RN.

Interactioni

Subunit structurei

Forms homooligomers and heterooligomers with STIM2. Interacts (via the transmembrane region and the SOAR/CAD domain) with SPPL3; the interaction promotes the binding of STIM1 to ORAI1. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and ORAI1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Interacts with ASPH. Interacts with SLC35G1; intracellular Ca2+-dependent. May interact with ATP1A1, ATP2A2, ATP2B1, ATP2B4, KPNB1 and XPO1; through SLC35G1. Interacts with TMEM203. Interacts with TMEM110/STIMATE, promoting STIM1 conformational switch. Interacts with TMEM178A.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP84903. 1 interaction.
STRINGi10116.ENSRNOP00000027685.

Structurei

3D structure databases

ProteinModelPortaliP84903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 9836EF-handSequence analysisAdd
BLAST
Domaini132 – 19059SAMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni334 – 444111SOAR/CADBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili248 – 442195By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi642 – 6454Microtubule tip localization signal

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity
The STIM1 Orai1-activating region/CRAC-activating domain (SOAR/CAD) mediates interaction with ORAI1 to activate the channel.By similarity

Sequence similaritiesi

Contains 1 EF-hand domain.Sequence analysis
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
GeneTreeiENSGT00390000000214.
HOGENOMiHOG000261647.
HOVERGENiHBG054652.
InParanoidiP84903.
KOiK16059.
PhylomeDBiP84903.
TreeFamiTF313487.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
IPR030463. STM1.
[Graphical view]
PANTHERiPTHR15136:SF9. PTHR15136:SF9. 1 hit.
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVCARLALW LLWGLLLHQG QSLSHSHSEK NTGASSGATS EESTEAEFCR
60 70 80 90 100
IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD
110 120 130 140 150
PTVKHSTFHG EDKLISVEDL WKAWKASEVY NWTVDEVIQW LITYVELPQY
160 170 180 190 200
EETFRKLQLT GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF
210 220 230 240 250
GPPLLTRHNH LKDFMLVVSI VIGVGGCWFA YIQNRYSKEH MKKMMKDLEG
260 270 280 290 300
LHRAEQSLHD LQERLHKAQE EHRTVEVEKV HLEKKLRDEI NLAKQEAQRL
310 320 330 340 350
KELREGTENE RSRQKYAEEE LEQVREALRK AEKELESHSS WYAPEALQKW
360 370 380 390 400
LQLTHEVEVQ YYNIKKQNAE RQLLVAKEGA EKIKKKRNTL FGTFHVAHSS
410 420 430 440 450
SLDDVDHKIL TAKQALSEVT AALRERLHRW QQIEILCGFQ IVNNPGIHSL
460 470 480 490 500
VAALNIDPSW MGSTRPNPAH FIMTDDVDDM DEEIVSPLSM QSPSLQSSVR
510 520 530 540 550
QRLTEPQHGL GSQRDLTHSD SESSLHTSDR QRVAPKPPQM GRAADEALNA
560 570 580 590 600
TSSNGSHRLI EGVHPGSLVE KLPDSPALAK KTILALNHGL DKAHSLMELN
610 620 630 640 650
PSVPPGGSPL LDSSHSHSPS SPDPDTPSPV GDSRALQGSR NTRIPHLAGK
660 670 680
KAMAEEDNGS IGEETDSSPG RKKFPLKIFK KPLKK
Length:685
Mass (Da):77,449
Last modified:September 5, 2006 - v1
Checksum:iFD2DA28427D2E41E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000423 Genomic DNA. No translation available.
AABR03002698 Genomic DNA. No translation available.
RefSeqiNP_001101966.2. NM_001108496.2.
UniGeneiRn.106771.

Genome annotation databases

EnsembliENSRNOT00000027685; ENSRNOP00000027685; ENSRNOG00000020425.
GeneIDi361618.
KEGGirno:361618.
UCSCiRGD:1306831. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000423 Genomic DNA. No translation available.
AABR03002698 Genomic DNA. No translation available.
RefSeqiNP_001101966.2. NM_001108496.2.
UniGeneiRn.106771.

3D structure databases

ProteinModelPortaliP84903.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP84903. 1 interaction.
STRINGi10116.ENSRNOP00000027685.

PTM databases

iPTMnetiP84903.
PhosphoSiteiP84903.

Proteomic databases

PaxDbiP84903.
PRIDEiP84903.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027685; ENSRNOP00000027685; ENSRNOG00000020425.
GeneIDi361618.
KEGGirno:361618.
UCSCiRGD:1306831. rat.

Organism-specific databases

CTDi6786.
RGDi1306831. Stim1.

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
GeneTreeiENSGT00390000000214.
HOGENOMiHOG000261647.
HOVERGENiHBG054652.
InParanoidiP84903.
KOiK16059.
PhylomeDBiP84903.
TreeFamiTF313487.

Enzyme and pathway databases

ReactomeiR-RNO-5578775. Ion homeostasis.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

PROiP84903.

Gene expression databases

BgeeiENSRNOG00000020425.
ExpressionAtlasiP84903. baseline and differential.
GenevisibleiP84903. RN.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
IPR030463. STM1.
[Graphical view]
PANTHERiPTHR15136:SF9. PTHR15136:SF9. 1 hit.
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTIM1_RAT
AccessioniPrimary (citable) accession number: P84903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.