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Protein

Aralkylamine dehydrogenase heavy chain

Gene

aauB

Organism
Alcaligenes faecalis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes primary aromatic amines and, more slowly, some long-chain aliphatic amines, but not methylamine or ethylamine. Uses azurin as an electron acceptor to transfer electrons from the reduced tryptophylquinone cofactor.4 Publications

Catalytic activityi

ArCH2NH2 + H2O + 2 azurin = ArCHO + NH3 + 2 reduced azurin.1 Publication

Enzyme regulationi

Irreversibly inhibited by phenylhydrazine, hydroxylamine, semicarbazide, hydrazine and aminoguanidine. Reversibly inhibited by isonicotinic acid hydrazide (isoniazid) and isonicotinic acid 2-isopropyl hydrazide (iproniazid).1 Publication

Kineticsi

The enzyme is substrate inhibited at high substrate concentrations (Ki=1.08 mM for tyramine).

  1. KM=5.4 µM for tyramine1 Publication
  1. Vmax=17 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Substrate3 Publications1
Binding sitei57Substrate3 Publications1
Binding sitei81Substrate3 Publications1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16553.
BRENDAi1.4.9.2. 232.
SABIO-RKP84888.

Names & Taxonomyi

Protein namesi
Recommended name:
Aralkylamine dehydrogenase heavy chain (EC:1.4.9.2)
Alternative name(s):
Aromatic amine dehydrogenase
Short name:
AADH
Gene namesi
Name:aauB1 Publication
OrganismiAlcaligenes faecalis
Taxonomic identifieri511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAlcaligenes

Subcellular locationi

  • Periplasm 2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 255 PublicationsAdd BLAST25
ChainiPRO_000028791026 – 390Aralkylamine dehydrogenase heavy chain5 PublicationsAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi182 ↔ 1993 Publications

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Heterotetramer of two light and two heavy chains. Binds two azurin molecules per heterotetramer.4 Publications

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi43 – 45Combined sources3
Beta strandi46 – 51Combined sources6
Helixi54 – 59Combined sources6
Beta strandi61 – 66Combined sources6
Turni67 – 69Combined sources3
Beta strandi72 – 77Combined sources6
Beta strandi80 – 86Combined sources7
Beta strandi90 – 100Combined sources11
Beta strandi103 – 106Combined sources4
Beta strandi108 – 116Combined sources9
Turni117 – 119Combined sources3
Beta strandi122 – 128Combined sources7
Helixi139 – 141Combined sources3
Beta strandi142 – 144Combined sources3
Beta strandi148 – 166Combined sources19
Turni167 – 170Combined sources4
Beta strandi171 – 176Combined sources6
Helixi177 – 179Combined sources3
Beta strandi182 – 187Combined sources6
Beta strandi191 – 199Combined sources9
Beta strandi202 – 209Combined sources8
Beta strandi213 – 220Combined sources8
Turni227 – 229Combined sources3
Beta strandi240 – 247Combined sources8
Beta strandi250 – 256Combined sources7
Beta strandi258 – 261Combined sources4
Beta strandi263 – 270Combined sources8
Helixi273 – 277Combined sources5
Beta strandi284 – 286Combined sources3
Beta strandi288 – 291Combined sources4
Turni292 – 295Combined sources4
Beta strandi296 – 304Combined sources9
Beta strandi315 – 321Combined sources7
Turni322 – 325Combined sources4
Beta strandi326 – 332Combined sources7
Beta strandi338 – 342Combined sources5
Turni343 – 346Combined sources4
Beta strandi347 – 351Combined sources5
Beta strandi356 – 360Combined sources5
Beta strandi362 – 365Combined sources4
Beta strandi367 – 372Combined sources6
Beta strandi381 – 384Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AGLX-ray1.40A/B30-389[»]
2AGWX-ray1.45A/B30-389[»]
2AGXX-ray2.20A/B30-389[»]
2AGYX-ray1.10A/B30-389[»]
2AGZX-ray1.60A/B30-389[»]
2AH0X-ray1.45A/B30-389[»]
2AH1X-ray1.20A/B30-389[»]
2H3XX-ray2.50A/D1-390[»]
2H47X-ray2.60A/D/F/H1-390[»]
2HJ4X-ray1.80A/B30-389[»]
2HJBX-ray1.85A/B30-389[»]
2HKMX-ray1.50A/B29-389[»]
2HKRX-ray1.40A/B29-389[»]
2HXCX-ray1.45A/B30-389[»]
2I0RX-ray1.40A/B30-389[»]
2I0SX-ray1.40A/B31-389[»]
2I0TX-ray1.35A/B30-389[»]
2IAAX-ray1.95A/D1-390[»]
2IUPX-ray1.80A/B30-389[»]
2IUQX-ray1.50A/B30-389[»]
2IURX-ray1.30A/B30-389[»]
2IUVX-ray1.55A/B30-389[»]
2OIZX-ray1.05A/B30-389[»]
2OJYX-ray1.60A/B29-389[»]
2OK4X-ray1.45A/B30-389[»]
2OK6X-ray1.45A/B30-389[»]
2Q7QX-ray1.60A/B30-390[»]
ProteinModelPortaliP84888.
SMRiP84888.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84888.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK13372.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR009451. Metamine_DH_Hvc.
IPR011044. Quino_amine_DH_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF06433. Me-amine-dh_H. 1 hit.
[Graphical view]
SUPFAMiSSF50969. SSF50969. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKFKLTTA AAMLGLMVLA GGAQAQDKPR EVLTGGHSVS APQENRIYVM
60 70 80 90 100
DSVFMHLTES RVHVYDYTNG KFLGMVPTAF NGHVQVSNDG KKIYTMTTYH
110 120 130 140 150
ERITRGKRSD VVEVWDADKL TFEKEISLPP KRVQGLNYDG LFRQTTDGKF
160 170 180 190 200
IVLQNASPAT SIGIVDVAKG DYVEDVTAAA GCWSVIPQPN RPRSFMTICG
210 220 230 240 250
DGGLLTINLG EDGKVASQSR SKQMFSVKDD PIFIAPALDK DKAHFVSYYG
260 270 280 290 300
NVYSADFSGD EVKVDGPWSL LNDEDKAKNW VPGGYNLVGL HRASGRMYVF
310 320 330 340 350
MHPDGKEGTH KFPAAEIWVM DTKTKQRVAR IPGRDALSMT IDQQRNLMLT
360 370 380 390
LDGGNVNVYD ISQPEPKLLR TIEGAAEASL QVQFHPVGGV
Length:390
Mass (Da):42,925
Last modified:May 15, 2007 - v1
Checksum:i0AFCC1129CC44FAC
GO

Mass spectrometryi

Molecular mass is 40422±1 Da from positions 26 - 390. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302652 Genomic DNA. No translation available.
AM292628 Genomic DNA. Translation: CAL23524.1.
RefSeqiWP_009462712.1. NZ_KQ954451.1.

Genome annotation databases

KEGGiag:CAL23524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302652 Genomic DNA. No translation available.
AM292628 Genomic DNA. Translation: CAL23524.1.
RefSeqiWP_009462712.1. NZ_KQ954451.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AGLX-ray1.40A/B30-389[»]
2AGWX-ray1.45A/B30-389[»]
2AGXX-ray2.20A/B30-389[»]
2AGYX-ray1.10A/B30-389[»]
2AGZX-ray1.60A/B30-389[»]
2AH0X-ray1.45A/B30-389[»]
2AH1X-ray1.20A/B30-389[»]
2H3XX-ray2.50A/D1-390[»]
2H47X-ray2.60A/D/F/H1-390[»]
2HJ4X-ray1.80A/B30-389[»]
2HJBX-ray1.85A/B30-389[»]
2HKMX-ray1.50A/B29-389[»]
2HKRX-ray1.40A/B29-389[»]
2HXCX-ray1.45A/B30-389[»]
2I0RX-ray1.40A/B30-389[»]
2I0SX-ray1.40A/B31-389[»]
2I0TX-ray1.35A/B30-389[»]
2IAAX-ray1.95A/D1-390[»]
2IUPX-ray1.80A/B30-389[»]
2IUQX-ray1.50A/B30-389[»]
2IURX-ray1.30A/B30-389[»]
2IUVX-ray1.55A/B30-389[»]
2OIZX-ray1.05A/B30-389[»]
2OJYX-ray1.60A/B29-389[»]
2OK4X-ray1.45A/B30-389[»]
2OK6X-ray1.45A/B30-389[»]
2Q7QX-ray1.60A/B30-390[»]
ProteinModelPortaliP84888.
SMRiP84888.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAL23524.

Phylogenomic databases

KOiK13372.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16553.
BRENDAi1.4.9.2. 232.
SABIO-RKP84888.

Miscellaneous databases

EvolutionaryTraceiP84888.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR009451. Metamine_DH_Hvc.
IPR011044. Quino_amine_DH_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF06433. Me-amine-dh_H. 1 hit.
[Graphical view]
SUPFAMiSSF50969. SSF50969. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAAUB_ALCFA
AccessioniPrimary (citable) accession number: P84888
Secondary accession number(s): Q0VKG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.