P84865 (MT1_NECLU) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 2, 2010.
Version 15.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Metallothionein |
| Organism | Nectria lugdunensis (Aquatic fungus) (Heliscus lugdunensis) |
| Taxonomic identifier | 57155 [NCBI] |
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria |
Protein attributes
| Sequence length | 24 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Metallothioneins have a high content of cysteine residues that bind various heavy metals. Ref.1 |
| Induction | By cadmium. Ref.1 |
| Post-translational modification | Contains 4 disulfide bonds Probable. |
| Sequence similarities | Belongs to the metallothionein superfamily. Type 8 family. |
| Mass spectrometry | Molecular mass is 2321.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of apo-metallothionein with intact disulfide bonds. Ref.1 Molecular mass is 2329.7 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of apo-metallothionein treated with acid to cleave disulfide bonds. Ref.1 Molecular mass is 2431.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of metallothionein with intact disulfide bonds chelating one cadmium ion. Ref.1 Molecular mass is 2541.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of metallothionein with intact disulfide bonds chelating 2 cadmium ions. Ref.1 |
Ontologies
| Keywords | |
|---|---|
| Ligand | Cadmium Metal-binding Metal-thiolate cluster |
| PTM | Disulfide bond |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular_function | metal chelating activity Inferred from direct assay Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Peptide | 1 – 24 | 24 | Metallothionein Ref.1 | PRO_0000244521 | |||||
Sites | |||||||||
| Metal binding | 3 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 5 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 8 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 10 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 14 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 17 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 19 | 1 | Cadmium Ref.1 | ||||||
| Metal binding | 22 | 1 | Cadmium Ref.1 | ||||||
Sequences
References
| [1] | "Cadmium induces a novel metallothionein and phytochelatin 2 in an aquatic fungus." Jaeckel P., Krauss G., Menge S., Schierhorn A., Ruecknagel P., Krauss G.J. Biochem. Biophys. Res. Commun. 333:150-155(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, INDUCTION, MASS SPECTROMETRY, DISULFIDE BONDS, CADMIUM BINDING. Tissue: Mycelium. |
Cross-references
Entry information
| Entry name | MT1_NECLU | ||||||||
| Accession | Primary (citable) accession number: P84865 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Metallothioneins Classification of metallothioneins and list of entries |
| SIMILARITY comments Index of protein domains and families |