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P84865 (MT1_NECLU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 2, 2010. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metallothionein
OrganismNectria lugdunensis (Aquatic fungus) (Heliscus lugdunensis)
Taxonomic identifier57155 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeNectria

Protein attributes

Sequence length24 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Metallothioneins have a high content of cysteine residues that bind various heavy metals. Ref.1

Induction

By cadmium. Ref.1

Post-translational modification

Contains 4 disulfide bonds Probable.

Sequence similarities

Belongs to the metallothionein superfamily. Type 8 family.

Mass spectrometry

Molecular mass is 2321.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of apo-metallothionein with intact disulfide bonds. Ref.1

Molecular mass is 2329.7 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of apo-metallothionein treated with acid to cleave disulfide bonds. Ref.1

Molecular mass is 2431.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of metallothionein with intact disulfide bonds chelating one cadmium ion. Ref.1

Molecular mass is 2541.5 Da from positions 1 - 24. Determined by MALDI. The measured mass is that of metallothionein with intact disulfide bonds chelating 2 cadmium ions. Ref.1

Ontologies

Keywords
   LigandCadmium
Metal-binding
Metal-thiolate cluster
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Molecular_functionmetal chelating activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 2424Metallothionein Ref.1
PRO_0000244521

Sites

Metal binding31Cadmium Ref.1
Metal binding51Cadmium Ref.1
Metal binding81Cadmium Ref.1
Metal binding101Cadmium Ref.1
Metal binding141Cadmium Ref.1
Metal binding171Cadmium Ref.1
Metal binding191Cadmium Ref.1
Metal binding221Cadmium Ref.1

Sequences

Sequence LengthMass (Da)Tools
P84865 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 6568E5C8BE87E8FF

FASTA242,331
        10         20 
SPCTCSTCNC AGACNSCSCT SCSH 

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References

[1]"Cadmium induces a novel metallothionein and phytochelatin 2 in an aquatic fungus."
Jaeckel P., Krauss G., Menge S., Schierhorn A., Ruecknagel P., Krauss G.J.
Biochem. Biophys. Res. Commun. 333:150-155(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, INDUCTION, MASS SPECTROMETRY, DISULFIDE BONDS, CADMIUM BINDING.
Tissue: Mycelium.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameMT1_NECLU
AccessionPrimary (citable) accession number: P84865
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 2, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Metallothioneins

Classification of metallothioneins and list of entries