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Protein

Mitochondrial fission 1 protein

Gene

Fis1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein complex binding Source: RGD
  • receptor binding Source: Ensembl

GO - Biological processi

  • autophagy of mitochondrion Source: Ensembl
  • calcium-mediated signaling using intracellular calcium source Source: Ensembl
  • cellular response to glucose stimulus Source: RGD
  • cellular response to lipid Source: RGD
  • cellular response to peptide Source: RGD
  • cellular response to thapsigargin Source: RGD
  • cellular response to toxic substance Source: RGD
  • mitochondrial fission Source: UniProtKB
  • mitochondrial fragmentation involved in apoptotic process Source: RGD
  • mitochondrial fusion Source: UniProtKB
  • mitochondrion morphogenesis Source: Ensembl
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: Ensembl
  • peroxisome fission Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • positive regulation of cytosolic calcium ion concentration Source: Ensembl
  • positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
  • positive regulation of mitochondrial calcium ion concentration Source: Ensembl
  • positive regulation of mitochondrial fission Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of protein targeting to membrane Source: UniProtKB
  • protein homooligomerization Source: RGD
  • protein targeting to mitochondrion Source: Ensembl
  • release of cytochrome c from mitochondria Source: Ensembl
  • response to flavonoid Source: RGD
  • response to hypobaric hypoxia Source: RGD
  • response to muscle activity Source: RGD

Keywordsi

Biological processApoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial fission 1 protein
Alternative name(s):
FIS1 homolog
Short name:
rFis1
Tetratricopeptide repeat protein 11
Short name:
TPR repeat protein 11
Gene namesi
Name:Fis1By similarity
Synonyms:Ttc11Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1306668. Fis1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 122CytoplasmicSequence analysisAdd BLAST122
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 152Mitochondrial intermembraneSequence analysis9

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-84; A-91; A-97 and A-98. 1 Publication1
Mutagenesisi84L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-91; A-97 and A-98. 1 Publication1
Mutagenesisi91L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-97 and A-98. 1 Publication1
Mutagenesisi97L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-98. 1 Publication1
Mutagenesisi98L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-97. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002339481 – 152Mitochondrial fission 1 proteinAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei10PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by MARCH5.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP84817.
PRIDEiP84817.

PTM databases

iPTMnetiP84817.
PhosphoSitePlusiP84817.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001420.
GenevisibleiP84817. RN.

Interactioni

Subunit structurei

Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5. Interacts with MIEF1. Interacts with PEX11A, PEX11B and PEX11G.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • receptor binding Source: Ensembl

Protein-protein interaction databases

BioGridi252608. 1 interactor.
MINTiMINT-4581071.
STRINGi10116.ENSRNOP00000061895.

Structurei

3D structure databases

ProteinModelPortaliP84817.
SMRiP84817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 104TPRSequence analysisAdd BLAST34

Domaini

The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.

Sequence similaritiesi

Belongs to the FIS1 family.Curated

Keywords - Domaini

TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3364. Eukaryota.
ENOG4111PPH. LUCA.
GeneTreeiENSGT00390000000592.
HOGENOMiHOG000165386.
HOVERGENiHBG081530.
InParanoidiP84817.
KOiK17969.
OMAiRKGIMIL.
OrthoDBiEOG091G0YFW.
PhylomeDBiP84817.
TreeFamiTF315180.

Family and domain databases

CDDicd12212. Fis1. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR016543. Fis1.
IPR033745. Fis1_cytosol.
IPR028061. Fis1_TPR_C.
IPR028058. Fis1_TPR_N.
IPR011990. TPR-like_helical_dom.
PANTHERiPTHR13247. PTHR13247. 1 hit.
PfamiView protein in Pfam
PF14853. Fis1_TPR_C. 1 hit.
PF14852. Fis1_TPR_N. 1 hit.
PIRSFiPIRSF008835. TPR_repeat_11_Fis1. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P84817-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAVLNELVS VEDLKNFERK FQSEQAAGSV SKSTQFEYAW CLVRSKYNDD
60 70 80 90 100
IRRGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT
110 120 130 140 150
EPQNNQAKEL ERLIDKAMKK DGLVGMAIVG GMALGVAGLA GLIGLAVSKS

KS
Length:152
Mass (Da):16,995
Last modified:May 2, 2006 - v1
Checksum:i50068E0C0148B1B0
GO
Isoform 2 (identifier: P84817-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEAVLNELVSVEDLK → MPRDEAAR
     121-152: DGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS → GDRPHAVCSS...CGGECGQGRG

Show »
Length:179
Mass (Da):20,246
Checksum:i09E996033486C0FA
GO
Isoform 3 (identifier: P84817-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEAVLNELVSVEDLK → MPRDEAAR

Note: No experimental confirmation available.
Show »
Length:145
Mass (Da):16,251
Checksum:i6D024F4B7CC6C17D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0396251 – 15MEAVL…VEDLK → MPRDEAAR in isoform 2 and isoform 3. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_039626121 – 152DGLVG…SKSKS → GDRPHAVCSSSSLRPGPWEH CPHPLHTHSPLSNLPSKGLG SPGAPTLRLYIQVPLFCGGE CGQGRG in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC111745 Genomic DNA. No translation available.
CH473973 Genomic DNA. Translation: EDM13301.1.
BC167057 mRNA. Translation: AAI67057.1.
RefSeqiNP_001099389.1. NM_001105919.1. [P84817-3]
XP_006249184.1. XM_006249122.3. [P84817-2]
XP_006249185.1. XM_006249123.3. [P84817-1]
UniGeneiRn.1298.

Genome annotation databases

EnsembliENSRNOT00000001924; ENSRNOP00000001924; ENSRNOG00000001420. [P84817-1]
ENSRNOT00000063901; ENSRNOP00000061895; ENSRNOG00000001420. [P84817-2]
ENSRNOT00000083471; ENSRNOP00000075023; ENSRNOG00000001420. [P84817-3]
GeneIDi288584.
KEGGirno:288584.
UCSCiRGD:1306668. rat. [P84817-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFIS1_RAT
AccessioniPrimary (citable) accession number: P84817
Secondary accession number(s): B2RZ80, D4A5K3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: September 27, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families