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P84817

- FIS1_RAT

UniProt

P84817 - FIS1_RAT

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Protein

Mitochondrial fission 1 protein

Gene

Fis1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission (By similarity).By similarity

GO - Biological processi

  1. calcium-mediated signaling using intracellular calcium source Source: Ensembl
  2. mitochondrial fission Source: UniProtKB
  3. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
  4. mitochondrial fusion Source: UniProtKB
  5. mitochondrion degradation Source: Ensembl
  6. mitochondrion morphogenesis Source: Ensembl
  7. negative regulation of endoplasmic reticulum calcium ion concentration Source: Ensembl
  8. peroxisome fission Source: UniProtKB
  9. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  10. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  11. positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
  12. positive regulation of mitochondrial calcium ion concentration Source: Ensembl
  13. positive regulation of mitochondrial fission Source: UniProtKB
  14. positive regulation of protein targeting to membrane Source: UniProtKB
  15. protein homooligomerization Source: Ensembl
  16. protein targeting to mitochondrion Source: Ensembl
  17. release of cytochrome c from mitochondria Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial fission 1 protein
Alternative name(s):
FIS1 homolog
Short name:
rFis1
Tetratricopeptide repeat protein 11
Short name:
TPR repeat protein 11
Gene namesi
Name:Fis1By similarity
Synonyms:Ttc11Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi1306668. Fis1.

Subcellular locationi

Mitochondrion outer membrane 1 Publication; Single-pass membrane protein 1 Publication. Peroxisome membrane 1 Publication; Single-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: UniProtKB
  2. integral component of peroxisomal membrane Source: UniProtKB
  3. peroxisome Source: HGNC
  4. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-84; A-91; A-97 and A-98. 1 Publication
Mutagenesisi84 – 841L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-91; A-97 and A-98. 1 Publication
Mutagenesisi91 – 911L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-97 and A-98. 1 Publication
Mutagenesisi97 – 971L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-98. 1 Publication
Mutagenesisi98 – 981L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-97. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Mitochondrial fission 1 proteinPRO_0000233948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Ubiquitinated by MARCH5.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiP84817.
PRIDEiP84817.

Expressioni

Gene expression databases

GenevestigatoriP84817.

Interactioni

Subunit structurei

Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5 (By similarity). Interacts with MIEF1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi252608. 1 interaction.
MINTiMINT-4581071.
STRINGi10116.ENSRNOP00000060173.

Structurei

3D structure databases

ProteinModelPortaliP84817.
SMRiP84817. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 122122CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini144 – 1529Mitochondrial intermembraneSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati71 – 10434TPRSequence AnalysisAdd
BLAST

Domaini

The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.

Sequence similaritiesi

Belongs to the FIS1 family.Curated
Contains 1 TPR repeat.Sequence Analysis

Keywords - Domaini

TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG235677.
GeneTreeiENSGT00390000000592.
HOGENOMiHOG000165386.
HOVERGENiHBG081530.
InParanoidiP84817.
KOiK17969.
OMAiEIFRTSP.
OrthoDBiEOG73V6NH.
PhylomeDBiP84817.
TreeFamiTF315180.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR016543. Fis1.
IPR028061. Fis1_TPR_C.
IPR028058. Fis1_TPR_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13247. PTHR13247. 1 hit.
PfamiPF14853. Fis1_TPR_C. 1 hit.
PF14852. Fis1_TPR_N. 1 hit.
[Graphical view]
PIRSFiPIRSF008835. TPR_repeat_11_Fis1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P84817-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAVLNELVS VEDLKNFERK FQSEQAAGSV SKSTQFEYAW CLVRSKYNDD
60 70 80 90 100
IRRGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT
110 120 130 140 150
EPQNNQAKEL ERLIDKAMKK DGLVGMAIVG GMALGVAGLA GLIGLAVSKS

KS
Length:152
Mass (Da):16,995
Last modified:May 2, 2006 - v1
Checksum:i50068E0C0148B1B0
GO
Isoform 2 (identifier: P84817-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEAVLNELVSVEDLK → MPRDEAAR
     121-152: DGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS → GDRPHAVCSS...CGGECGQGRG

Show »
Length:179
Mass (Da):20,246
Checksum:i09E996033486C0FA
GO
Isoform 3 (identifier: P84817-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEAVLNELVSVEDLK → MPRDEAAR

Note: No experimental confirmation available.

Show »
Length:145
Mass (Da):16,251
Checksum:i6D024F4B7CC6C17D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1515MEAVL…VEDLK → MPRDEAAR in isoform 2 and isoform 3. 1 PublicationVSP_039625Add
BLAST
Alternative sequencei121 – 15232DGLVG…SKSKS → GDRPHAVCSSSSLRPGPWEH CPHPLHTHSPLSNLPSKGLG SPGAPTLRLYIQVPLFCGGE CGQGRG in isoform 2. 1 PublicationVSP_039626Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC111745 Genomic DNA. No translation available.
CH473973 Genomic DNA. Translation: EDM13301.1.
BC167057 mRNA. Translation: AAI67057.1.
RefSeqiNP_001099389.1. NM_001105919.1. [P84817-3]
XP_006249184.1. XM_006249122.2. [P84817-2]
XP_006249185.1. XM_006249123.2. [P84817-1]
UniGeneiRn.1298.

Genome annotation databases

EnsembliENSRNOT00000001924; ENSRNOP00000001924; ENSRNOG00000001420. [P84817-1]
ENSRNOT00000063901; ENSRNOP00000061895; ENSRNOG00000001420. [P84817-2]
GeneIDi288584.
KEGGirno:288584.
UCSCiRGD:1306668. rat. [P84817-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC111745 Genomic DNA. No translation available.
CH473973 Genomic DNA. Translation: EDM13301.1 .
BC167057 mRNA. Translation: AAI67057.1 .
RefSeqi NP_001099389.1. NM_001105919.1. [P84817-3 ]
XP_006249184.1. XM_006249122.2. [P84817-2 ]
XP_006249185.1. XM_006249123.2. [P84817-1 ]
UniGenei Rn.1298.

3D structure databases

ProteinModelPortali P84817.
SMRi P84817. Positions 1-145.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 252608. 1 interaction.
MINTi MINT-4581071.
STRINGi 10116.ENSRNOP00000060173.

Proteomic databases

PaxDbi P84817.
PRIDEi P84817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001924 ; ENSRNOP00000001924 ; ENSRNOG00000001420 . [P84817-1 ]
ENSRNOT00000063901 ; ENSRNOP00000061895 ; ENSRNOG00000001420 . [P84817-2 ]
GeneIDi 288584.
KEGGi rno:288584.
UCSCi RGD:1306668. rat. [P84817-1 ]

Organism-specific databases

CTDi 51024.
RGDi 1306668. Fis1.

Phylogenomic databases

eggNOGi NOG235677.
GeneTreei ENSGT00390000000592.
HOGENOMi HOG000165386.
HOVERGENi HBG081530.
InParanoidi P84817.
KOi K17969.
OMAi EIFRTSP.
OrthoDBi EOG73V6NH.
PhylomeDBi P84817.
TreeFami TF315180.

Miscellaneous databases

NextBioi 628350.
PROi P84817.

Gene expression databases

Genevestigatori P84817.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
InterProi IPR016543. Fis1.
IPR028061. Fis1_TPR_C.
IPR028058. Fis1_TPR_N.
IPR011990. TPR-like_helical_dom.
[Graphical view ]
PANTHERi PTHR13247. PTHR13247. 1 hit.
Pfami PF14853. Fis1_TPR_C. 1 hit.
PF14852. Fis1_TPR_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF008835. TPR_repeat_11_Fis1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein."
    Jofuku A., Ishihara N., Mihara K.
    Biochem. Biophys. Res. Commun. 333:650-659(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-77; LEU-84; LEU-91; LEU-97 AND LEU-98.
    Tissue: Liver1 Publication.
  2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spleen.

Entry informationi

Entry nameiFIS1_RAT
AccessioniPrimary (citable) accession number: P84817
Secondary accession number(s): B2RZ80, D4A5K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3