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P84817

- FIS1_RAT

UniProt

P84817 - FIS1_RAT

Protein

Mitochondrial fission 1 protein

Gene

Fis1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission By similarity.By similarity

    GO - Biological processi

    1. calcium-mediated signaling using intracellular calcium source Source: Ensembl
    2. mitochondrial fission Source: UniProtKB
    3. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
    4. mitochondrial fusion Source: UniProtKB
    5. mitochondrion degradation Source: Ensembl
    6. mitochondrion morphogenesis Source: Ensembl
    7. negative regulation of endoplasmic reticulum calcium ion concentration Source: Ensembl
    8. peroxisome fission Source: UniProtKB
    9. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    10. positive regulation of cytosolic calcium ion concentration Source: Ensembl
    11. positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
    12. positive regulation of mitochondrial calcium ion concentration Source: Ensembl
    13. positive regulation of mitochondrial fission Source: UniProtKB
    14. positive regulation of protein targeting to membrane Source: UniProtKB
    15. protein homooligomerization Source: Ensembl
    16. protein targeting to mitochondrion Source: Ensembl
    17. release of cytochrome c from mitochondria Source: Ensembl

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial fission 1 protein
    Alternative name(s):
    FIS1 homolog
    Short name:
    rFis1
    Tetratricopeptide repeat protein 11
    Short name:
    TPR repeat protein 11
    Gene namesi
    Name:Fis1By similarity
    Synonyms:Ttc11Imported
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi1306668. Fis1.

    Subcellular locationi

    Mitochondrion outer membrane 1 Publication; Single-pass membrane protein 1 Publication. Peroxisome membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: UniProtKB
    2. integral component of peroxisomal membrane Source: UniProtKB
    3. peroxisome Source: HGNC
    4. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi77 – 771L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-84; A-91; A-97 and A-98. 1 Publication
    Mutagenesisi84 – 841L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-91; A-97 and A-98. 1 Publication
    Mutagenesisi91 – 911L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-97 and A-98. 1 Publication
    Mutagenesisi97 – 971L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-98. 1 Publication
    Mutagenesisi98 – 981L → A: Loss of mitochondrial fission-stimulating activity; when associated with A-77; A-84; A-91 and A-97. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Mitochondrial fission 1 proteinPRO_0000233948Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Ubiquitinated by MARCH5.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PaxDbiP84817.
    PRIDEiP84817.

    Expressioni

    Gene expression databases

    GenevestigatoriP84817.

    Interactioni

    Subunit structurei

    Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5 By similarity. Interacts with MIEF1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi252608. 1 interaction.
    MINTiMINT-4581071.
    STRINGi10116.ENSRNOP00000060173.

    Structurei

    3D structure databases

    ProteinModelPortaliP84817.
    SMRiP84817. Positions 1-145.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 122122CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini144 – 1529Mitochondrial intermembraneSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati71 – 10434TPRSequence AnalysisAdd
    BLAST

    Domaini

    The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.

    Sequence similaritiesi

    Belongs to the FIS1 family.Curated
    Contains 1 TPR repeat.Sequence Analysis

    Keywords - Domaini

    TPR repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG235677.
    GeneTreeiENSGT00390000000592.
    HOGENOMiHOG000165386.
    HOVERGENiHBG081530.
    InParanoidiP84817.
    KOiK17969.
    OMAiEIFRTSP.
    OrthoDBiEOG73V6NH.
    PhylomeDBiP84817.
    TreeFamiTF315180.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    InterProiIPR016543. Fis1.
    IPR028061. Fis1_TPR_C.
    IPR028058. Fis1_TPR_N.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PANTHERiPTHR13247. PTHR13247. 1 hit.
    PfamiPF14853. Fis1_TPR_C. 1 hit.
    PF14852. Fis1_TPR_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008835. TPR_repeat_11_Fis1. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P84817-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAVLNELVS VEDLKNFERK FQSEQAAGSV SKSTQFEYAW CLVRSKYNDD    50
    IRRGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT 100
    EPQNNQAKEL ERLIDKAMKK DGLVGMAIVG GMALGVAGLA GLIGLAVSKS 150
    KS 152
    Length:152
    Mass (Da):16,995
    Last modified:May 2, 2006 - v1
    Checksum:i50068E0C0148B1B0
    GO
    Isoform 2 (identifier: P84817-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: MEAVLNELVSVEDLK → MPRDEAAR
         121-152: DGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS → GDRPHAVCSS...CGGECGQGRG

    Show »
    Length:179
    Mass (Da):20,246
    Checksum:i09E996033486C0FA
    GO
    Isoform 3 (identifier: P84817-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: MEAVLNELVSVEDLK → MPRDEAAR

    Note: No experimental confirmation available.

    Show »
    Length:145
    Mass (Da):16,251
    Checksum:i6D024F4B7CC6C17D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1515MEAVL…VEDLK → MPRDEAAR in isoform 2 and isoform 3. 1 PublicationVSP_039625Add
    BLAST
    Alternative sequencei121 – 15232DGLVG…SKSKS → GDRPHAVCSSSSLRPGPWEH CPHPLHTHSPLSNLPSKGLG SPGAPTLRLYIQVPLFCGGE CGQGRG in isoform 2. 1 PublicationVSP_039626Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC111745 Genomic DNA. No translation available.
    CH473973 Genomic DNA. Translation: EDM13301.1.
    BC167057 mRNA. Translation: AAI67057.1.
    RefSeqiNP_001099389.1. NM_001105919.1. [P84817-3]
    XP_006249184.1. XM_006249122.1. [P84817-2]
    XP_006249185.1. XM_006249123.1. [P84817-1]
    UniGeneiRn.1298.

    Genome annotation databases

    EnsembliENSRNOT00000001924; ENSRNOP00000001924; ENSRNOG00000001420. [P84817-1]
    ENSRNOT00000063901; ENSRNOP00000061895; ENSRNOG00000001420. [P84817-2]
    GeneIDi288584.
    KEGGirno:288584.
    UCSCiRGD:1306668. rat. [P84817-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC111745 Genomic DNA. No translation available.
    CH473973 Genomic DNA. Translation: EDM13301.1 .
    BC167057 mRNA. Translation: AAI67057.1 .
    RefSeqi NP_001099389.1. NM_001105919.1. [P84817-3 ]
    XP_006249184.1. XM_006249122.1. [P84817-2 ]
    XP_006249185.1. XM_006249123.1. [P84817-1 ]
    UniGenei Rn.1298.

    3D structure databases

    ProteinModelPortali P84817.
    SMRi P84817. Positions 1-145.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 252608. 1 interaction.
    MINTi MINT-4581071.
    STRINGi 10116.ENSRNOP00000060173.

    Proteomic databases

    PaxDbi P84817.
    PRIDEi P84817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001924 ; ENSRNOP00000001924 ; ENSRNOG00000001420 . [P84817-1 ]
    ENSRNOT00000063901 ; ENSRNOP00000061895 ; ENSRNOG00000001420 . [P84817-2 ]
    GeneIDi 288584.
    KEGGi rno:288584.
    UCSCi RGD:1306668. rat. [P84817-1 ]

    Organism-specific databases

    CTDi 51024.
    RGDi 1306668. Fis1.

    Phylogenomic databases

    eggNOGi NOG235677.
    GeneTreei ENSGT00390000000592.
    HOGENOMi HOG000165386.
    HOVERGENi HBG081530.
    InParanoidi P84817.
    KOi K17969.
    OMAi EIFRTSP.
    OrthoDBi EOG73V6NH.
    PhylomeDBi P84817.
    TreeFami TF315180.

    Miscellaneous databases

    NextBioi 628350.
    PROi P84817.

    Gene expression databases

    Genevestigatori P84817.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    InterProi IPR016543. Fis1.
    IPR028061. Fis1_TPR_C.
    IPR028058. Fis1_TPR_N.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    PANTHERi PTHR13247. PTHR13247. 1 hit.
    Pfami PF14853. Fis1_TPR_C. 1 hit.
    PF14852. Fis1_TPR_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008835. TPR_repeat_11_Fis1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein."
      Jofuku A., Ishihara N., Mihara K.
      Biochem. Biophys. Res. Commun. 333:650-659(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-77; LEU-84; LEU-91; LEU-97 AND LEU-98.
      Tissue: Liver1 Publication.
    2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Spleen.

    Entry informationi

    Entry nameiFIS1_RAT
    AccessioniPrimary (citable) accession number: P84817
    Secondary accession number(s): B2RZ80, D4A5K3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3