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P84801

- GRFIN_GRISQ

UniProt

P84801 - GRFIN_GRISQ

Protein

Griffithsin

Gene
N/A
Organism
Griffithsia sp. (strain Q66D336) (Red alga)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mixed specificity lectin with anti-HIV activity. Binds to HIV envelope glycoproteins, including exterior membrane glycoprotein gp120, and inhibits viral entry into cells. Binding to gp120 is dependent on gp120 being glycosylated, and is inhibited by mannose, glucose and N-acetylglucosamine.1 Publication

    GO - Molecular functioni

    1. carbohydrate binding Source: UniProtKB
    2. glucose binding Source: UniProtKB
    3. mannose binding Source: UniProtKB
    4. N-acetylgalactosamine binding Source: UniProtKB
    5. protein binding Source: IntAct

    Keywords - Ligandi

    Lectin, Mannose-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Griffithsin
    Short name:
    GRFT
    OrganismiGriffithsia sp. (strain Q66D336) (Red alga)
    Taxonomic identifieri373036 [NCBI]
    Taxonomic lineageiEukaryotaRhodophytaFlorideophyceaeCeramialesCeramiaceaeGriffithsia

    Subcellular locationi

    GO - Cellular componenti

    1. cell Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121121GriffithsinPRO_0000228815Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    envQ757602EBI-8453570,EBI-8453491From a different organism.

    Protein-protein interaction databases

    DIPiDIP-29130N.
    IntActiP84801. 1 interaction.
    MINTiMINT-8414712.

    Structurei

    Secondary structure

    1
    121
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Beta strandi12 – 154
    Beta strandi20 – 3415
    Beta strandi37 – 404
    Beta strandi44 – 463
    Beta strandi58 – 7619
    Beta strandi81 – 866
    Beta strandi90 – 12031

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GTYX-ray1.30A/B1-121[»]
    2GUCX-ray1.79A/B1-121[»]
    2GUDX-ray0.94A/B1-121[»]
    2GUEX-ray2.02A/B1-121[»]
    2GUXX-ray2.00A1-121[»]
    2HYQX-ray2.00A/B1-121[»]
    2HYRX-ray1.51A/B1-121[»]
    2NU5X-ray1.56A/B1-121[»]
    2NUOX-ray1.50A/B1-121[»]
    3LKYX-ray1.11A1-121[»]
    3LL0X-ray1.70A1-121[»]
    3LL1X-ray0.97A1-119[»]
    3LL2X-ray0.97A1-121[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP84801.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the jacalin lectin family.Curated

    Family and domain databases

    Gene3Di2.100.10.30. 1 hit.
    InterProiIPR001229. Mannose-bd_lectin.
    [Graphical view]
    PfamiPF01419. Jacalin. 1 hit.
    [Graphical view]
    SMARTiSM00915. Jacalin. 1 hit.
    [Graphical view]
    SUPFAMiSSF51101. SSF51101. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P84801-1 [UniParc]FASTAAdd to Basket

    « Hide

    SLTHRKFGGS GGSPFSGLSS IAVRSGSYLD XIIIDGVHHG GSGGNLSPTF    50
    TFGSGEYISN MTIRSGDYID NISFETNMGR RFGPYGGSGG SANTLSNVKV 100
    IQINGSAGDY LDSLDIYYEQ Y 121
    Length:121
    Mass (Da):12,731
    Last modified:March 21, 2006 - v1
    Checksum:iF89843AF6DE5049D
    GO

    Mass spectrometryi

    Molecular mass is 12770.05 Da from positions 1 - 121. Determined by ESI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GTY X-ray 1.30 A/B 1-121 [» ]
    2GUC X-ray 1.79 A/B 1-121 [» ]
    2GUD X-ray 0.94 A/B 1-121 [» ]
    2GUE X-ray 2.02 A/B 1-121 [» ]
    2GUX X-ray 2.00 A 1-121 [» ]
    2HYQ X-ray 2.00 A/B 1-121 [» ]
    2HYR X-ray 1.51 A/B 1-121 [» ]
    2NU5 X-ray 1.56 A/B 1-121 [» ]
    2NUO X-ray 1.50 A/B 1-121 [» ]
    3LKY X-ray 1.11 A 1-121 [» ]
    3LL0 X-ray 1.70 A 1-121 [» ]
    3LL1 X-ray 0.97 A 1-119 [» ]
    3LL2 X-ray 0.97 A 1-121 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29130N.
    IntActi P84801. 1 interaction.
    MINTi MINT-8414712.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P84801.

    Family and domain databases

    Gene3Di 2.100.10.30. 1 hit.
    InterProi IPR001229. Mannose-bd_lectin.
    [Graphical view ]
    Pfami PF01419. Jacalin. 1 hit.
    [Graphical view ]
    SMARTi SM00915. Jacalin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51101. SSF51101. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp."
      Mori T., O'Keefe B.R., Sowder R.C. II, Bringans S., Gardella R., Berg S., Cochran P., Turpin J.A., Buckheit R.W. Jr., McMahon J.B., Boyd M.R.
      J. Biol. Chem. 280:9345-9353(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGRFIN_GRISQ
    AccessioniPrimary (citable) accession number: P84801
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3