P84801 (GRFIN_GRISQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Griffithsin Short name=GRFT
Organism	Griffithsia sp. (strain Q66D336) (Red alga)
Taxonomic identifier	373036 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Rhodophyta › Florideophyceae › Ceramiales › Ceramiaceae › Griffithsia

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mixed specificity lectin with anti-HIV activity. Binds to HIV envelope glycoproteins, including exterior membrane glycoprotein gp120, and inhibits viral entry into cells. Binding to gp120 is dependent on gp120 being glycosylated, and is inhibited by mannose, glucose and N-acetylglucosamine. Ref.1
Sequence similarities	Belongs to the jacalin lectin family.
Mass spectrometry	Molecular mass is 12770.05 Da from positions 1 - 121. Determined by ESI. Ref.1

Ontologies

Keywords
Ligand	Lectin Mannose-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cell Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	N-acetylgalactosamine binding Inferred from direct assay Ref.1. Source: UniProtKB glucose binding Inferred from direct assay Ref.1. Source: UniProtKB mannose binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 121

121

Griffithsin

PRO_0000228815

Secondary structure

1

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121

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Beta strand

12 – 15

4

Beta strand

20 – 34

15

Beta strand

37 – 40

4

Beta strand

44 – 46

3

Beta strand

58 – 76

19

Beta strand

81 – 86

6

Beta strand

90 – 120

31

Sequences

	Sequence		Length	Mass (Da)
	P84801 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: F89843AF6DE5049D	FASTA	121	12,731
	10 20 30 40 50 60 SLTHRKFGGS GGSPFSGLSS IAVRSGSYLD XIIIDGVHHG GSGGNLSPTF TFGSGEYISN 70 80 90 100 110 120 MTIRSGDYID NISFETNMGR RFGPYGGSGG SANTLSNVKV IQINGSAGDY LDSLDIYYEQ Y

References

[1]	"Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp." Mori T., O'Keefe B.R., Sowder R.C. II, Bringans S., Gardella R., Berg S., Cochran P., Turpin J.A., Buckheit R.W. Jr., McMahon J.B., Boyd M.R. J. Biol. Chem. 280:9345-9353(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GTY	X-ray	1.30	A/B	1-121	[»]
2GUC	X-ray	1.79	A/B	1-121	[»]
2GUD	X-ray	0.94	A/B	1-121	[»]
2GUE	X-ray	2.02	A/B	1-121	[»]
2GUX	X-ray	2.00	A	1-121	[»]
2HYQ	X-ray	2.00	A/B	1-121	[»]
2HYR	X-ray	1.51	A/B	1-121	[»]
2NU5	X-ray	1.56	A/B	1-121	[»]
2NUO	X-ray	1.50	A/B	1-121	[»]
3LKY	X-ray	1.11	A	1-121	[»]
3LL0	X-ray	1.70	A	1-121	[»]
3LL1	X-ray	0.97	A	3-118	[»]
3LL2	X-ray	0.97	A	3-121	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29130N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.100.10.30. 1 hit.

InterPro

IPR001229. Mannose-bd_lectin.
[Graphical view]

Pfam

PF01419. Jacalin. 1 hit.
[Graphical view]

SMART

SM00915. Jacalin. 1 hit.
[Graphical view]

SUPFAM

SSF51101. Mannose-bd_lectin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P84801.

Entry information

Entry name

GRFIN_GRISQ

Accession

Primary (citable) accession number: P84801

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	March 6, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)