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P84781 (ITR3_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin inhibitor 3
Alternative name(s):
SOTI III
Trypsin inhibitor III
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length37 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Trypsin inhibitor. Ref.1

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Belongs to the Mirabilis serine proteinase inhibitor family. Ref.1

Mass spectrometry

Molecular mass is 3838.4 Da from positions 1 - 37. Determined by ESI. Ref.1

Ontologies

Keywords
   DomainKnottin
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3737Trypsin inhibitor 3 Ref.1
PRO_0000292939

Sites

Site32 – 332Reactive bond for trypsin By similarity UniProtKB P84779

Amino acid modifications

Disulfide bond4 ↔ 21 By similarity UniProtKB P84779
Disulfide bond11 ↔ 25 By similarity UniProtKB P84779
Disulfide bond20 ↔ 36 By similarity UniProtKB P84779

Secondary structure

......... 37
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P84781 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: D1F1F78D4EF22274

FASTA373,837
        10         20         30 
EDKCSPSGAI CSGFGPPEQC CSGACVPHPI LRIFVCQ 

« Hide

References

[1]"Trypsin inhibitors from the garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) seeds: isolation, characterization and chemical synthesis."
Kowalska J., Pszczola K., Wilimowska-Pelc A., Lorenc-Kubis I., Zuziak E., Lugowski M., Legowska A., Kwiatkowska A., Sleszynska M., Lesner A., Walewska A., Zablotna E., Rolka K., Wilusz T.
Phytochemistry 68:1487-1496(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.
Tissue: Seed.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AOQX-ray2.00D/E/F1-37[»]
4AORX-ray1.70D/E/F1-37[»]
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI90.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameITR3_SPIOL
AccessionPrimary (citable) accession number: P84781
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references