Potassium channel toxin alpha-KTx 15.6 precursor - Tityus discrepans (Venezuelan scorpion)

Functionⁱ

Irreversibly blocks the A-type voltage-gated potassium channels in rat cerebellum granular cells (190 nM induce 50% inhibitory effect, PubMed:15369825) (IC₅₀=190 nM, PubMed:18280256).2 Publications

Miscellaneous

Does not block ERG1/Kv11.1/KCNH2 currents.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	51	Basic residue of the functional dyadBy similarity		1
Siteⁱ	60	Aromatic residue of the functional dyadBy similarity		1

GO - Molecular functionⁱ

potassium channel inhibitor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 15369825

Complete GO annotation...

GO - Biological processⁱ

defense response Source: InterPro
envenomation resulting in negative regulation of platelet aggregation in other organism
Source: CACAO
Inferred from direct assayⁱ
- 21322750
pathogenesis
Source: UniProtKB
Inferred from direct assayⁱ
- 15369825

Complete GO annotation...

Keywordsⁱ

Molecular function	Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Molecular function

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Protein family/group databases

TCDBⁱ	8.B.8.1.1. the -ktx15 scorpion toxin (-ktx15) family.

TCDBⁱ

8.B.8.1.1. the -ktx15 scorpion toxin (-ktx15) family.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Potassium channel toxin alpha-KTx 15.61 Publication Alternative name(s): Discrepin1 Publication
Organismⁱ	Tityus discrepans (Venezuelan scorpion)
Taxonomic identifierⁱ	57059 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Subcellular locationⁱ

Secreted
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "Discrepin, a new peptide of the sub-family alpha-ktx15, isolated from the scorpion Tityus discrepans irreversibly blocks K+ -channels (IA currents) of cerebellum granular cells."
  D'Suze G., Batista C.V.F., Frau A., Murgia A.R., Zamudio F.Z., Sevcik C., Possani L.D., Prestipino G.
  Arch. Biochem. Biophys. 430:256-263(2004) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 24-61, FUNCTION, SUBCELLULAR LOCATION, PYROGLUTAMATE FORMATION AT GLN-24, NOMENCLATURE.

GO - Cellular componentⁱ

extracellular region
Source: UniProtKB
Inferred from direct assayⁱ
- 15369825

Complete GO annotation...

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	29	V → K: Large increase in blocking A-type potassium currents (IC(50)=51 nM) (keeps irreversible activity). No change in activity towards hERG currents. 2 Publications	1
Mutagenesisⁱ	42	I → R: Decrease in blocking A-type potassium currents (IC(50)=335 nM). Decrease in blocking A-type potassium currents (IC(50)=235 nM); when associated with K-43. Decrease in blocking A-type potassium currents (IC(50)=335 nM). Large decrease in blocking A-type potassium currents (IC(50)=764 nM); when associated with K-43 and V-44. Blocks hERG currents by 18.5% at 2.4 uM. No change in activity towards hERG currents; when associated with K-43. Blocks hERG currents with an IC(50)=3.5 uM; when associated with K-43 and V-44. 2 Publications	1
Mutagenesisⁱ	43	D → K: Increase in blocking A-type potassium currents (IC(50)=96 nM). Decrease in blocking A-type potassium currents (IC(50)=335 nM). Decrease in blocking A-type potassium currents (IC(50)=235 nM); when associated with R-42. Large decrease in blocking A-type potassium currents (IC(50)=764 nM); when associated with R-42 and V-44. Blocks hERG currents by 20.2% at 2.4 uM. No change in activity towards hERG currents; when associated with R-42. Blocks hERG currents with an IC(50)=3.5 uM; when associated with R-42 and V-44. 2 Publications	1
Mutagenesisⁱ	44	R → V: Large decrease in blocking A-type potassium currents (IC(50)=764 nM); when associated with R-42 and K-43. Blocks hERG currents with an IC(50)=3.5 uM; when associated with R-42 and K-43. 2 Publications	1
Mutagenesisⁱ	58	T → V: Decrease in blocking A-type potassium currents (IC(50)=261 nM). 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 23	1 PublicationAdd BLAST		23
ChainⁱPRO_0000224192	24 – 61	Potassium channel toxin alpha-KTx 15.61 PublicationAdd BLAST		38

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	24	Pyrrolidone carboxylic acid2 Publications	1
Disulfide bondⁱ	31 ↔ 52	1 Publication
Disulfide bondⁱ	37 ↔ 57	1 Publication
Disulfide bondⁱ	41 ↔ 59	1 Publication

Keywords - PTMⁱ

Disulfide bond, Pyrrolidone carboxylic acid

Expressionⁱ

Tissue specificityⁱ

Expressed by the venom gland.Curated

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	25 – 30	Combined sources	6
Helixⁱ	34 – 44	Combined sources	11
Beta strandⁱ	50 – 59	Combined sources	10

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2AXK	NMR	-	A	25-61	[»]
SMRⁱ	P84777.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P84777.

EvolutionaryTraceⁱ

P84777.

Family & Domainsⁱ

Domainⁱ

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).1 Publication

Sequence similaritiesⁱ

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 15 subfamily.Curated

Keywords - Domainⁱ

Signal

Family and domain databases

Gene3Dⁱ	3.30.30.10. 1 hit.
InterProⁱ	View protein in InterPro IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00451. Toxin_2. 1 hit.
SUPFAMⁱ	SSF57095. SSF57095. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01138. SCORP_SHORT_TOXIN. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P84777-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKAFYGMLVI FILCSTCYIS VDSQIDTNVK CSGSSKCVKI CIDRYNTRGA 
        60 
KCINGRCTCY P

Length:61

Mass (Da):6,787

Last modified:April 20, 2010 - v2

Checksum:ⁱCE0F426D6B00A494

GO

Mass spectrometryⁱ

Molecular mass is 4177 Da from positions 24 - 61. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	FN392270 mRNA. Translation: CAY61912.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

FN392270 mRNA. Translation: CAY61912.1.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	FN392270 mRNA. Translation: CAY61912.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

FN392270 mRNA. Translation: CAY61912.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2AXK	NMR	-	A	25-61	[»]
SMRⁱ	P84777.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

TCDBⁱ	8.B.8.1.1. the -ktx15 scorpion toxin (-ktx15) family.

TCDBⁱ

8.B.8.1.1. the -ktx15 scorpion toxin (-ktx15) family.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P84777.

EvolutionaryTraceⁱ

P84777.

Family and domain databases

Gene3Dⁱ	3.30.30.10. 1 hit.
InterProⁱ	View protein in InterPro IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00451. Toxin_2. 1 hit.
SUPFAMⁱ	SSF57095. SSF57095. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01138. SCORP_SHORT_TOXIN. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KA156_TITDI
Accessionⁱ	P84777Primary (citable) accession number: P84777 Secondary accession number(s): C9X4J2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
	Last sequence update:	April 20, 2010
	Last modified:	November 2, 2016
	This is version 39 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Animal Toxin Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Direct protein sequencing

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Scorpion potassium channel toxins
Nomenclature of scorpion potassium channel toxins and list of entries
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P84777 (KA156_TITDI)

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Potassium channel toxin alpha-KTx 15.6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Mass spectrometryⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ