Reviewed, UniProtKB/Swiss-Prot P84744 (KAX95_BUTOC)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 9.5 Alternative name(s):     Neurotoxin Kbot1
Organism	Buthus occitanus tunetanus (Common European scorpion)
Taxonomic identifier	6871 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Buthus

Protein attributes

Sequence length	28 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds and inhibits voltage-gated potassium channels, with varying affinities for Kv1.2 > Kv1.3 > Kv1.1. Also inhibits calcium-activated potassium channels. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland. Ref.1
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 9 subfamily.
Mass spectrometry	Molecular mass is 2942.58 Da from positions 1 - 28. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of potassium ion transport Ref.1 Inferred from direct assay. Source: UniProtKB pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	potassium channel inhibitor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 28	28	Potassium channel toxin alpha-KTx 9.5		PRO_0000045198
Amino acid modifications
	Modified residue	28	1	Valine amide Probable Ref.1
	Disulfide bond	3 ↔ 19		By similarity UniProtKB Q9NJP7
	Disulfide bond	6 ↔ 24		By similarity UniProtKB Q9NJP7
	Disulfide bond	10 ↔ 26		By similarity UniProtKB Q9NJP7

Sequences

	Sequence		Length	Mass (Da)
	P84744-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: DD72AD78A710B98B	FASTA	28	2,949
	10 20 VGCEECPMHC KGKHAVPTCD DGVCNCNV

References

[1]

"Kbot1, a three disulfide bridges toxin from Buthus occitanus tunetanus venom highly active on both SK and Kv channels." Mahjoubi-Boubaker B., Crest M., Khalifa R.B., Ayeb M.E., Kharrat R. Peptides 25:637-645(2004) [PubMed: 15165720] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008911. Toxin_6. [Graphical view]
Pfam	PF05453. Toxin_6. 1 hit. [Graphical view]
PROSITE	PS01138. SCORP_SHORT_TOXIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX95_BUTOC

Accession

Primary (citable) accession number: P84744

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	November 24, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)