P84722 (ALFC_PINST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Putative fructose-bisphosphate aldolase, chloroplastic EC=4.1.2.13 Alternative name(s): PS6
Organism	Pinus strobus (Eastern white pine)
Taxonomic identifier	3348 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Strobus

Protein attributes

Sequence length	71 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Subcellular location	Plastid › chloroplast.
Miscellaneous	On the 2D-gel the determined pI of this protein is: 5.6, its MW is: 25.2 kDa. Ref.1
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.
Caution	The order of the peptides shown is unknown. Ref.1

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Ligand	Schiff base
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›71	›71	Putative fructose-bisphosphate aldolase, chloroplastic		PRO_0000240610
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	29 – 30	2
	Non-adjacent residues	37 – 38	2
	Non-adjacent residues	42 – 43	2
	Non-adjacent residues	52 – 53	2
	Non-adjacent residues	59 – 60	2
	Non-adjacent residues	65 – 66	2
	Non-terminal residue	1	1
	Non-terminal residue	71	1

Sequences

	Sequence		Length	Mass (Da)
	P84722 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 77840B64DE586391	FASTA	71	7,141
	10 20 30 40 50 60 TVVSIPNGPS ALAVKGILAM DESNATCGKG SYADELVTFE VACXGLDVAA SRYSEELVKA 70 PSALTSVVTP G

References

[1]

"Proteomic comparison of needles from blister rust-resistant and susceptible Pinus strobus seedlings reveals upregulation of putative disease resistance proteins." Smith J.A., Blanchette R.A., Burnes T.A., Jacobs J.J., Higgins L., Witthuhn B.A., David A.J., Gillman J.H. Mol. Plant Microbe Interact. 19:150-160(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Leaf.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00158. ALDOLASE_CLASS_I. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC_PINST

Accession

Primary (citable) accession number: P84722

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 31, 2011
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program