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P84715

- ANF39_ORNAN

UniProt

P84715 - ANF39_ORNAN

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Protein

C-type natriuretic peptide

Gene
N/A
Organism
Ornithorhynchus anatinus (Duckbill platypus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Venom component with vasorelaxant activity. In vitro stimulates the production of cGMP in rat aortic smooth muscle cells and histamine release from rat peritoneal mast cells. Induces relaxation of isolated rat uterus. Induces local edema following subplantar injection into rat hind paw. Forms voltage-dependent cation channels which are weakly selective for potassium relative to sodium, and whose conductance decreases with increasing dehydration energy of the monovalent cation. The activity of the fast cation channels is calcium dependent and is characterized by short bursts of current separated by long periods of inactivation.5 Publications
Venom peptide 1 induces slow and continuous calcium influx in IMR-32 human neuroblastoma cells. Venom peptide 4 weakly induces calcium influx in IMR-32 human neuroblastoma cells while venom peptide 2 was not observed to induce calcium influx.

GO - Molecular functioni

  1. potassium channel activity Source: UniProtKB-KW
  2. sodium channel activity Source: UniProtKB-KW

GO - Biological processi

  1. cGMP biosynthetic process Source: Ensembl
  2. growth plate cartilage chondrocyte differentiation Source: Ensembl
  3. growth plate cartilage chondrocyte proliferation Source: Ensembl
  4. negative regulation of meiotic cell cycle Source: Ensembl
  5. negative regulation of oocyte maturation Source: Ensembl
  6. positive regulation of mast cell degranulation in other organism Source: UniProtKB
  7. post-embryonic development Source: Ensembl
  8. receptor guanylyl cyclase signaling pathway Source: Ensembl
  9. regulation of blood pressure Source: UniProtKB-KW
  10. regulation of cAMP metabolic process Source: Ensembl
  11. regulation of cGMP metabolic process Source: Ensembl
  12. regulation of multicellular organism growth Source: Ensembl
  13. single organism reproductive process Source: Ensembl
  14. vasodilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hypotensive agent, Ion channel, Neurotoxin, Potassium channel, Sodium channel, Toxin, Vasoactive, Vasodilator

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Transport

Keywords - Ligandi

Potassium, Sodium

Names & Taxonomyi

Protein namesi
OrganismiOrnithorhynchus anatinus (Duckbill platypus)
Taxonomic identifieri9258 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMonotremataOrnithorhynchidaeOrnithorhynchus
ProteomesiUP000002279: Unplaced

Subcellular locationi

Secreted 3 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 44221 PublicationPRO_0000393552Add
BLAST
Peptidei45 – 6319Venom peptide 10PRO_0000393553Add
BLAST
Peptidei45 – 6319Venom peptide 11PRO_0000393554Add
BLAST
Peptidei45 – 6218Venom peptide 8PRO_0000393555Add
BLAST
Peptidei47 – 6216Venom peptide 7PRO_0000393556Add
BLAST
Peptidei51 – 6212Venom peptide 6PRO_0000393557Add
BLAST
Peptidei52 – 6312Venom peptide 9PRO_0000393558Add
BLAST
Peptidei52 – 6211Venom peptide 5PRO_0000393559Add
BLAST
Propeptidei64 – 8219PRO_0000393560Add
BLAST
Peptidei83 – 12139C-type natriuretic peptide 39PRO_0000045067Add
BLAST
Peptidei83 – 919Venom peptide 4PRO_0000393561
Peptidei83 – 897Venom peptide 3PRO_0000393562
Peptidei85 – 917Venom peptide 1PRO_0000393563
Peptidei86 – 916Venom peptide 2PRO_0000393564

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki45 ↔ 46Alanine isoaspartyl cyclopeptide (Ala-Asn); in form venom peptide 10
Modified residuei84 – 841D-leucine; in forms ovCNP-39b, venom peptide 3 and venom peptide 42 Publications

Post-translational modificationi

Stereoinversion of L-Leu-84 (in ovCNP-39a) to D-Leu-84 (in ovCNP-39b).2 Publications

Keywords - PTMi

D-amino acid

Expressioni

Tissue specificityi

Expressed by the venom gland.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi9258.ENSOANP00000019499.

Family & Domainsi

Sequence similaritiesi

In the C-terminal section; belongs to the natriuretic peptide family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG43160.

Family and domain databases

InterProiIPR002406. C_natriurtcpep.
IPR000663. Natr_peptide.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00713. CNATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84715-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHLSHLLAWA LLLTLLSLRA EAKPPSPQPQ VPRSPGDEAS EAVAANGGGK
60 70 80 90 100
KGDKEPKGDR PRLLRELRLD TRSRGSRGVW TRLLHDHPNP RKYKPANKKG
110 120
LSKGCFGLKL DRIGSTSGLG C
Length:121
Mass (Da):13,148
Last modified:April 20, 2010 - v2
Checksum:i4DB01932ABB50B64
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901P → N AA sequence (PubMed:19928958)Curated

Mass spectrometryi

Molecular mass is 4207.9 Da from positions 83 - 121. Determined by MALDI. 1 Publication
Molecular mass is 4208.3 Da from positions 83 - 121. Determined by MALDI. 1 Publication
Molecular mass is 4212 Da from positions 83 - 121. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAPN01060770 Genomic DNA. No translation available.
AAPN01060771 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAPN01060770 Genomic DNA. No translation available.
AAPN01060771 Genomic DNA. No translation available.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9258.ENSOANP00000019499.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG43160.

Family and domain databases

InterProi IPR002406. C_natriurtcpep.
IPR000663. Natr_peptide.
[Graphical view ]
Pfami PF00212. ANP. 1 hit.
[Graphical view ]
PRINTSi PR00713. CNATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTi SM00183. NAT_PEP. 1 hit.
[Graphical view ]
PROSITEi PS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the platypus reveals unique signatures of evolution."
    Warren W.C., Hillier L.W., Marshall Graves J.A., Birney E., Ponting C.P., Grutzner F., Belov K., Miller W., Clarke L., Chinwalla A.T., Yang S.P., Heger A., Locke D.P., Miethke P., Waters P.D., Veyrunes F., Fulton L., Fulton B.
    , Graves T., Wallis J., Puente X.S., Lopez-Otin C., Ordonez G.R., Eichler E.E., Chen L., Cheng Z., Deakin J.E., Alsop A., Thompson K., Kirby P., Papenfuss A.T., Wakefield M.J., Olender T., Lancet D., Huttley G.A., Smit A.F., Pask A., Temple-Smith P., Batzer M.A., Walker J.A., Konkel M.K., Harris R.S., Whittington C.M., Wong E.S., Gemmell N.J., Buschiazzo E., Vargas Jentzsch I.M., Merkel A., Schmitz J., Zemann A., Churakov G., Kriegs J.O., Brosius J., Murchison E.P., Sachidanandam R., Smith C., Hannon G.J., Tsend-Ayush E., McMillan D., Attenborough R., Rens W., Ferguson-Smith M., Lefevre C.M., Sharp J.A., Nicholas K.R., Ray D.A., Kube M., Reinhardt R., Pringle T.H., Taylor J., Jones R.C., Nixon B., Dacheux J.L., Niwa H., Sekita Y., Huang X., Stark A., Kheradpour P., Kellis M., Flicek P., Chen Y., Webber C., Hardison R., Nelson J., Hallsworth-Pepin K., Delehaunty K., Markovic C., Minx P., Feng Y., Kremitzki C., Mitreva M., Glasscock J., Wylie T., Wohldmann P., Thiru P., Nhan M.N., Pohl C.S., Smith S.M., Hou S., Nefedov M., de Jong P.J., Renfree M.B., Mardis E.R., Wilson R.K.
    Nature 453:175-183(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Duck-billed platypus venom peptides induce Ca2+ influx in neuroblastoma cells."
    Kita M., Black D.S., Ohno O., Yamada K., Kigoshi H., Uemura D.
    J. Am. Chem. Soc. 131:18038-18039(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 45-63 AND 83-91, FUNCTION, D-AMINO ACID AT LEU-84, CYCLIZATION.
  3. "A C-type natriuretic peptide from the venom of the platypus (Ornithorhynchus anatinus): structure and pharmacology."
    de Plater G.M., Martin R.L., Milburn P.J.
    Comp. Biochem. Physiol. 120C:99-110(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 83-121, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Venom.
  4. "A pharmacological and biochemical investigation of the venom from the platypus (Ornithorhynchus anatinus)."
    de Plater G., Martin R.L., Milburn P.J.
    Toxicon 33:157-169(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 83-92, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Venom1 Publication.
  5. "The natriuretic peptide (ovCNP-39) from platypus (Ornithorhynchus anatinus) venom relaxes the isolated rat uterus and promotes oedema and mast cell histamine release."
    de Plater G.M., Martin R.L., Milburn P.J.
    Toxicon 36:847-857(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Calcium dependence of C-type natriuretic peptide-formed fast K(+) channel."
    Kourie J.I.
    Am. J. Physiol. 277:C43-C50(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Characterization of a C-type natriuretic peptide (CNP-39)-formed cation-selective channel from platypus (Ornithorhynchus anatinus) venom."
    Kourie J.I.
    J. Physiol. (Lond.) 518:359-369(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "D-amino acid residue in the C-type natriuretic peptide from the venom of the mammal, Ornithorhynchus anatinus, the Australian platypus."
    Torres A.M., Menz I., Alewood P.F., Bansal P., Lahnstein J., Gallagher C.H., Kuchel P.W.
    FEBS Lett. 524:172-176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: D-AMINO ACID AT LEU-84.
  9. "Conformations of platypus venom C-type natriuretic peptide in aqueous solution and sodium dodecyl sulfate micelles."
    Torres A.M., Alewood D., Alewood P.F., Gallagher C.H., Kuchel P.W.
    Toxicon 40:711-719(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 83-121.

Entry informationi

Entry nameiANF39_ORNAN
AccessioniPrimary (citable) accession number: P84715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: April 20, 2010
Last modified: October 29, 2014
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3