Reviewed, UniProtKB/Swiss-Prot P84708 (CAI_PHAVU)

Names and origin

Protein names	Recommended name:     Chitinolytic alpha-amylase inhibitor PvCAI     EC=3.2.1.14
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Alpha-amylase inhibitor, active against Z.subfasciatus alpha-amylase (ZSA) but not porcine pancreatic alpha-amylase (PPA). Has chitinase activity. Ref.1
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Ref.1
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Mass spectrometry	Molecular mass is 33330±10 Da from positions 1 - ?. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Molecular function	Alpha-amylase inhibitor Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase inhibitor activity Ref.1 Inferred from direct assay. Source: UniProtKB chitinase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Chitinolytic alpha-amylase inhibitor PvCAI		PRO_0000077056
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P84708-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: A2BEE14FF9EE2F4C	FASTA	25	2,705
	10 20 HEDNAGIAVY WGQDAREGDL VTACN

References

[1]

"Identification of a novel bean alpha-amylase inhibitor with chitinolytic activity." Dayler C.S.A., Mendes P.A.M., Prates M.V., Bloch C. Jr., Franco O.L., Grossi-de-Sa M.F. FEBS Lett. 579:5616-5620(2005) [PubMed: 16213488] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, MASS SPECTROMETRY. Strain: G12953. Tissue: Cotyledon.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.14. 8.
Family and domain databases
PROSITE	PS01095. CHITINASE_18. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAI_PHAVU

Accession

Primary (citable) accession number: P84708

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)