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P84646

- SCX1_ODODO

UniProt

P84646 - SCX1_ODODO

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Protein

Alpha-toxin OD1

Gene
N/A
Organism
Odontobuthus doriae (Yellow Iranian scorpion)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Alpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. Mammalian sodium channels Nav1.7/SCN9A (EC(50)=4.5 nM), Nav1.4/SCN4A (EC(50)=9.6 nM), Nav1.6/SCN8A (EC(50)=30 nM) Nav1.5/SCN5A (only at micromolar concentrations), and insect sodium channel para/tipE (EC(50)=80 nM) are affected by this toxin.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei6 – 61Important for toxin potency but not for selectivity for individual Nav channel subtype (Nav1.6 and Nav1.7)

GO - Molecular functioni

  1. sodium channel inhibitor activity Source: UniProtKB

GO - Biological processi

  1. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-toxin OD1
OrganismiOdontobuthus doriae (Yellow Iranian scorpion)
Taxonomic identifieri342590 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeOdontobuthus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi6 – 61Y → F: Decrease in potency of about 10-fold, without affecting the selectivity for Nav1.4, Nav1.6 and Nav1.7. 1 Publication
Mutagenesisi9 – 113DDK → KPH: Increase in potency for Nav1.4 (1.6-fold) and Nav1.7 (2.3-fold) and decrease in potency for Nav1.6 (fold), resulting in a 40-fold increase of selectivity for Nav1.7 over Nav1.6. No change in potency for Nav1.4 and Nav1.6, and important increase in potency for Nav1.7 (11.7-fold); when associated with A-55. 1 Publication
Mutagenesisi11 – 111K → V: Increase in potency for Nav1.4 (5-fold) and Nav1.6 (7.8-fold) and decrease in potency for Nav1.7 (4.3-fold), resulting in a 5-fold increase of selectivity for Nav1.6 over Nav1.7. Increase in potency for Nav1.6 (16-fold), no change in potency for Nav1.7, and decrease in potency for Nav1.4 (1.6-fold); when associated with A-55. 1 Publication
Mutagenesisi51 – 511K → A: No change in potency for Nav1.4, Nav1.6 and Nav1.7. 1 Publication
Mutagenesisi55 – 551E → A: No change in potency for Nav1.4 and Nav1.7, and important decrease in potency for Nav1.6 (2.3-fold), resulting in a 13-fold increase of selectivity for Nav1.4 and Nav1.7 over Nav1.6. No change in potency for Nav1.4; Nav1.6, and important increase in potency for Nav1.7 (11.7-fold); when associated with 9-K--H-11. Increase in potency for Nav1.6 (16-fold), no change in potency for Nav1.7, and decrease in potency for Nav1.4 (1.6-fold); when associated with V-11. 1 Publication
Mutagenesisi55 – 551E → H: No change in potency for Nav1.4 and Nav1.7, and decrease in potency for Nav1.6 (1.7-fold). 1 Publication
Mutagenesisi60 – 601I → G: No change in potency for Nav1.4, and decrease in potency for Nav1.6 (1.6-fold) and Nav1.7 (2.6-fold). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6565Alpha-toxin OD1PRO_0000066788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi13 ↔ 641 Publication
Disulfide bondi17 ↔ 371 Publication
Disulfide bondi23 ↔ 471 Publication
Disulfide bondi27 ↔ 491 Publication
Modified residuei65 – 651Arginine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

1
65
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87
Helixi20 – 2910
Beta strandi33 – 397
Helixi41 – 433
Beta strandi45 – 539

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HHFX-ray1.80A1-65[»]
ProteinModelPortaliP84646.
SMRiP84646. Positions 2-64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 113Important for toxin selectivity for individual Nav channel subtype (Nav1.6 and Nav1.7), but not for toxin potency

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

P84646 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
GVRDAYIADD KNCVYTCASN GYCNTECTKN GAESGYCQWI GRYGNACWCI
60
KLPDEVPIRI PGKCR
Length:65
Mass (Da):7,215
Last modified:October 11, 2005 - v1
Checksum:iFF76362F0C7ACCE7
GO

Mass spectrometryi

Molecular mass is 7204.8 Da from positions 1 - 65. Determined by MALDI. With amidation.1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HHF X-ray 1.80 A 1-65 [» ]
ProteinModelPortali P84646.
SMRi P84646. Positions 2-64.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.30.10. 1 hit.
InterProi IPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view ]
Pfami PF00537. Toxin_3. 1 hit.
[Graphical view ]
PRINTSi PR00285. SCORPNTOXIN.
SMARTi SM00505. Knot1. 1 hit.
[Graphical view ]
SUPFAMi SSF57095. SSF57095. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "OD1, the first toxin isolated from the venom of the scorpion Odonthobuthus doriae active on voltage-gated Na+ channels."
    Jalali A., Bosmans F., Amininasab M., Clynen E., Cuypers E., Zaremirakabadi A., Sarbolouki M.N., Schoofs L., Vatanpour H., Tytgat J.
    FEBS Lett. 579:4181-4186(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, TOXIN TARGET, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AMIDATION AT ARG-65.
    Tissue: Venom1 Publication.
  2. "Potent modulation of the voltage-gated sodium channel Nav1.7 by OD1, a toxin from the scorpion Odonthobuthus doriae."
    Maertens C., Cuypers E., Amininasab M., Jalali A., Vatanpour H., Tytgat J.
    Mol. Pharmacol. 70:405-414(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOXIN TARGET.
  3. "Chemical Engineering and Structural and Pharmacological Characterization of the alpha-Scorpion Toxin OD1."
    Durek T., Vetter I., Wang C.I., Motin L., Knapp O., Adams D.J., Lewis R.J., Alewood P.F.
    ACS Chem. Biol. 8:1215-1222(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), SYNTHESIS, FUNCTION, TOXIN TARGET, DISULFIDE BONDS, MUTAGENESIS OF TYR-6; 9-ASP--LYS-11; LYS-11; LYS-51; GLU-55 AND ILE-60.

Entry informationi

Entry nameiSCX1_ODODO
AccessioniPrimary (citable) accession number: P84646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Mammalian sodium channels Nav1.2/SCN2A, Nav1.3/SCN3A, and Nav1.8/SCN10A are unaffected by high concentrations (PubMed:16038905 and PubMed:16641312).

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3