Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-toxin OD1

Gene
N/A
Organism
Odontobuthus doriae (Yellow Iranian scorpion)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels. The toxin affect mammalian sodium channels Nav1.7/SCN9A (EC(50)=4.5 nM), Nav1.4/SCN4A (EC(50)=9.6 nM), Nav1.6/SCN8A (EC(50)=30 nM), Nav1.5/SCN5A (only at micromolar concentrations), and insect sodium channel para/tipE (EC(50)=80 nM) (PubMed:16038905, PubMed:16641312, PubMed:23527544). In vivo, intraplantar administration of this toxin elicits pain behaviors, including licking and flinching of the hind paw (PubMed:26999206).3 Publications

Miscellaneous

Intraplantal administration of this protein is used as a pharmacological tool to establish a Nav1.7/SCN9A-mediated mouse model of pain.1 Publication
Mammalian sodium channels Nav1.2/SCN2A, Nav1.3/SCN3A, and Nav1.8/SCN10A are unaffected by high concentrations (PubMed:16038905 and PubMed:16641312).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei6Important for toxin potency but not for selectivity for individual Nav channel subtype (Nav1.6/SCN8A and Nav1.7/SCN9A)1 Publication1

GO - Molecular functioni

  • sodium channel inhibitor activity Source: UniProtKB
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-toxin OD11 Publication
OrganismiOdontobuthus doriae (Yellow Iranian scorpion)
Taxonomic identifieri342590 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeOdontobuthus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6Y → F: Decrease in potency for Nav1.4/SCN4A (17-fold), Nav1.6/SCN8A (8-fold), and Nav1.7/SCN9A (17-fold). 1 Publication1
Mutagenesisi9 – 11DDK → KPH: Increase in potency for Nav1.4/SCN4A (1.6-fold) and Nav1.7/SCN9A (2.3-fold) and decrease in potency for Nav1.6/SCN8A (3-fold), resulting in a 40-fold increase of selectivity for Nav1.7/SCN9A over Nav1.6/SCN8A. No change in potency for Nav1.4/SCN4A and Nav1.6/SCN8A, and important increase in potency for Nav1.7/SCN9A (11.7-fold); when associated with A-55. 1 Publication3
Mutagenesisi11K → V: Increase in potency for Nav1.4/SCN4A (5-fold) and Nav1.6/SCN8A (7.8-fold) and decrease in potency for Nav1.7/SCN9A (4.3-fold), resulting in a 5-fold increase of selectivity for Nav1.6/SCN8A over Nav1.7/SCN9A. Increase in potency for Nav1.6/SCN8A (16-fold), no change in potency for Nav1.7/SCN9A, and decrease in potency for Nav1.4/SCN4A (1.6-fold); when associated with A-55. 1 Publication1
Mutagenesisi51K → A: No change in potency for Nav1.4/SCN4A, Nav1.6/SCN8A and Nav1.7/SCN9A. 1 Publication1
Mutagenesisi55E → A: No change in potency for Nav1.4/SCN4A and Nav1.7/SCN9A, and important decrease in potency for Nav1.6/SCN8A (2.3-fold), resulting in a 13-fold increase of selectivity for Nav1.4/SCN4A and Nav1.7/SCN9A over Nav1.6/SCN8A. No change in potency for Nav1.4/SCN4A; Nav1.6/SCN8A, and important increase in potency for Nav1.7/SCN9A (11.7-fold); when associated with 9-K--H-11. Increase in potency for Nav1.6/SCN8A (16-fold), no change in potency for Nav1.7/SCN9A, and decrease in potency for Nav1.4/SCN4A (1.6-fold); when associated with V-11. 1 Publication1
Mutagenesisi55E → H: No change in potency for Nav1.4/SCN4A and Nav1.7/SCN9A, and decrease in potency for Nav1.6/SCN8A (1.7-fold). 1 Publication1
Mutagenesisi60I → G: No change in potency for Nav1.4/SCN4A, and decrease in potency for Nav1.6/SCN8A (1.6-fold) and Nav1.7/SCN9A (2.6-fold). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000667881 – 65Alpha-toxin OD11 PublicationAdd BLAST65

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi13 ↔ 641 PublicationImported
Disulfide bondi17 ↔ 371 PublicationImported
Disulfide bondi23 ↔ 471 PublicationImported
Disulfide bondi27 ↔ 491 PublicationImported
Modified residuei65Arginine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi20 – 29Combined sources10
Beta strandi33 – 39Combined sources7
Helixi41 – 43Combined sources3
Beta strandi45 – 53Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HHFX-ray1.80A1-65[»]
ProteinModelPortaliP84646
SMRiP84646
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 11Important for toxin selectivity for individual Nav channel subtype (Nav1.6/SCN8A and Nav1.7/SCN9A), but not for toxin potency1 Publication3

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10, 1 hit
InterProiView protein in InterPro
IPR003614 Scorpion_toxin-like
IPR036574 Scorpion_toxin-like_sf
IPR018218 Scorpion_toxinL
IPR002061 Scorpion_toxinL/defensin
PfamiView protein in Pfam
PF00537 Toxin_3, 1 hit
PRINTSiPR00285 SCORPNTOXIN
SMARTiView protein in SMART
SM00505 Knot1, 1 hit
SUPFAMiSSF57095 SSF57095, 1 hit

Sequencei

Sequence statusi: Complete.

P84646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GVRDAYIADD KNCVYTCASN GYCNTECTKN GAESGYCQWI GRYGNACWCI
60
KLPDEVPIRI PGKCR
Length:65
Mass (Da):7,215
Last modified:October 11, 2005 - v1
Checksum:iFF76362F0C7ACCE7
GO

Mass spectrometryi

Molecular mass is 7204.8 Da from positions 1 - 65. Determined by MALDI. With amidation.1 Publication

Similar proteinsi

Entry informationi

Entry nameiSCX1_ODODO
AccessioniPrimary (citable) accession number: P84646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: May 23, 2018
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health