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Protein

Dicer-like protein 4

Gene

DCL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the biogenesis of trans-acting small interfering RNAs (ta-siRNAs, derived from the TAS1, TAS2 or TAS3 endogenous transcripts) by cleaving small dsRNAs into 21-24 nucleotide ta-siRNAs. Functions with the dsRNA-binding protein DRB4 in ta-siRNAs processing. Acts in the RDR6/SGS3/DCL4/AGO7 ta-siRNA pathway involved in leaf developmental timing. Plays a role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL2 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. Required for the production of the 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs (ra-siRNAs) which derive from heterochromatin and DNA repeats such as transposons. Plays an important role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the cucumber mosaic virus (CMV), turnip crinkle virus (TCV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL4 function in RNA silencing. Does not seem to be involved in microRNAs (miRNAs) processing.11 Publications

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1330 – 13301Magnesium or manganeseBy similarity
Sitei1418 – 14181Important for activityBy similarity
Metal bindingi1422 – 14221Magnesium or manganeseBy similarity
Metal bindingi1425 – 14251Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi144 – 1518ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: TAIR
  • DNA-templated transcription, termination Source: TAIR
  • maintenance of DNA methylation Source: TAIR
  • production of lsiRNA involved in RNA interference Source: TAIR
  • production of siRNA involved in RNA interference Source: TAIR
  • production of ta-siRNAs involved in RNA interference Source: TAIR
  • RNA phosphodiester bond hydrolysis Source: GOC
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  • RNA virus induced gene silencing Source: TAIR
  • vegetative phase change Source: TAIR
  • virus induced gene silencing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

Plant defense, RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G20320-MONOMER.
ARA:GQT-2073-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 4 (EC:3.1.26.-)
Gene namesi
Name:DCL4
Ordered Locus Names:At5g20320
ORF Names:F5O24.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20320.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: TAIR
  • RISC complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17021702Dicer-like protein 4PRO_0000180475Add
BLAST

Proteomic databases

PaxDbiP84634.
PRIDEiP84634.

PTM databases

iPTMnetiP84634.

Expressioni

Gene expression databases

ExpressionAtlasiP84634. baseline and differential.
GenevisibleiP84634. AT.

Interactioni

Subunit structurei

Interacts with DRB4.1 Publication

Protein-protein interaction databases

BioGridi17430. 4 interactions.
IntActiP84634. 2 interactions.
MINTiMINT-6948367.
STRINGi3702.AT5G20320.1.

Structurei

Secondary structure

1
1702
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi655 – 66410Combined sources
Beta strandi667 – 6693Combined sources
Beta strandi678 – 6836Combined sources
Helixi686 – 6883Combined sources
Beta strandi690 – 6956Combined sources
Beta strandi705 – 7084Combined sources
Helixi713 – 73119Combined sources
Turni735 – 7373Combined sources
Helixi741 – 7444Combined sources
Turni748 – 7503Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KOUNMR-A651-752[»]
ProteinModelPortaliP84634.
SMRiP84634. Positions 122-645, 651-752, 1103-1251, 1290-1530.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84634.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 307177Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini475 – 629155Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini656 – 74893Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini927 – 1054128PAZPROSITE-ProRule annotationAdd
BLAST
Domaini1083 – 1251169RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1292 – 1436145RNase III 2PROSITE-ProRule annotationAdd
BLAST
Domaini1462 – 152867DRBM 1PROSITE-ProRule annotationAdd
BLAST
Domaini1621 – 169777DRBM 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi251 – 2544DECH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi89 – 10214Poly-SerAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
HOGENOMiHOG000083706.
InParanoidiP84634.
KOiK11592.
OMAiLDFWKCE.
PhylomeDBiP84634.

Family and domain databases

Gene3Di1.10.1520.10. 3 hits.
3.30.160.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 2 hits.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P84634-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRDEVDLSLT IPSKLLGKRD REQKNCEEEK NKNKKAKKQQ KDPILLHTSA
60 70 80 90 100
ATHKFLPPPL TMPYSEIGDD LRSLDFDHAD VSSDLHLTSS SSVSSFSSSS
110 120 130 140 150
SSLFSAAGTD DPSPKMEKDP RKIARRYQVE LCKKATEENV IVYLGTGCGK
160 170 180 190 200
THIAVMLIYE LGHLVLSPKK SVCIFLAPTV ALVEQQAKVI ADSVNFKVAI
210 220 230 240 250
HCGGKRIVKS HSEWEREIAA NEVLVMTPQI LLHNLQHCFI KMECISLLIF
260 270 280 290 300
DECHHAQQQS NHPYAEIMKV FYKSESLQRP RIFGMTASPV VGKGSFQSEN
310 320 330 340 350
LSKSINSLEN LLNAKVYSVE SNVQLDGFVS SPLVKVYYYR SALSDASQST
360 370 380 390 400
IRYENMLEDI KQRCLASLKL LIDTHQTQTL LSMKRLLKRS HDNLIYTLLN
410 420 430 440 450
LGLWGAIQAA KIQLNSDHNV QDEPVGKNPK SKICDTYLSM AAEALSSGVA
460 470 480 490 500
KDENASDLLS LAALKEPLFS RKLVQLIKIL SVFRLEPHMK CIIFVNRIVT
510 520 530 540 550
ARTLSCILNN LELLRSWKSD FLVGLSSGLK SMSRRSMETI LKRFQSKELN
560 570 580 590 600
LLVATKVGEE GLDIQTCCLV IRYDLPETVT SFIQSRGRAR MPQSEYAFLV
610 620 630 640 650
DSGNEKEMDL IENFKVNEDR MNLEITYRSS EETCPRLDEE LYKVHETGAC
660 670 680 690 700
ISGGSSISLL YKYCSRLPHD EFFQPKPEFQ FKPVDEFGGT ICRITLPANA
710 720 730 740 750
PISEIESSLL PSTEAAKKDA CLKAVHELHN LGVLNDFLLP DSKDEIEDEL
760 770 780 790 800
SDDEFDFDNI KGEGCSRGDL YEMRVPVLFK QKWDPSTSCV NLHSYYIMFV
810 820 830 840 850
PHPADRIYKK FGFFMKSPLP VEAETMDIDL HLAHQRSVSV KIFPSGVTEF
860 870 880 890 900
DNDEIRLAEL FQEIALKVLF ERGELIPDFV PLELQDSSRT SKSTFYLLLP
910 920 930 940 950
LCLHDGESVI SVDWVTIRNC LSSPIFKTPS VLVEDIFPPS GSHLKLANGC
960 970 980 990 1000
WNIDDVKNSL VFTTYSKQFY FVADICHGRN GFSPVKESST KSHVESIYKL
1010 1020 1030 1040 1050
YGVELKHPAQ PLLRVKPLCH VRNLLHNRMQ TNLEPQELDE YFIEIPPELS
1060 1070 1080 1090 1100
HLKIKGLSKD IGSSLSLLPS IMHRMENLLV AIELKHVLSA SIPEIAEVSG
1110 1120 1130 1140 1150
HRVLEALTTE KCHERLSLER LEVLGDAFLK FAVSRHLFLH HDSLDEGELT
1160 1170 1180 1190 1200
RRRSNVVNNS NLCRLAIKKN LQVYIRDQAL DPTQFFAFGH PCRVTCDEVA
1210 1220 1230 1240 1250
SKEVHSLNRD LGILESNTGE IRCSKGHHWL YKKTIADVVE ALVGAFLVDS
1260 1270 1280 1290 1300
GFKGAVKFLK WIGVNVDFES LQVQDACIAS RRYLPLTTRN NLETLENQLD
1310 1320 1330 1340 1350
YKFLHKGLLV QAFIHPSYNR HGGGCYQRLE FLGDAVLDYL MTSYFFTVFP
1360 1370 1380 1390 1400
KLKPGQLTDL RSLSVNNEAL ANVAVSFSLK RFLFCESIYL HEVIEDYTNF
1410 1420 1430 1440 1450
LASSPLASGQ SEGPRCPKVL GDLVESCLGA LFLDCGFNLN HVWTMMLSFL
1460 1470 1480 1490 1500
DPVKNLSNLQ ISPIKELIEL CQSYKWDREI SATKKDGAFT VELKVTKNGC
1510 1520 1530 1540 1550
CLTVSATGRN KREGTKKAAQ LMITNLKAHE NITTSHPLED VLKNGIRNEA
1560 1570 1580 1590 1600
KLIGYNEDPI DVVDLVGLDV ENLNILETFG GNSERSSSYV IRRGLPQAPS
1610 1620 1630 1640 1650
KTEDRLPQKA IIKAGGPSSK TAKSLLHETC VANCWKPPHF ECCEEEGPGH
1660 1670 1680 1690 1700
LKSFVYKVIL EVEDAPNMTL ECYGEARATK KGAAEHAAQA AIWCLKHSGF

LC
Length:1,702
Mass (Da):191,279
Last modified:February 8, 2011 - v2
Checksum:i7AA7647A6BA7F24D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ118423 mRNA. Translation: AAZ80387.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92830.1.
RefSeqiNP_197532.3. NM_122039.4. [P84634-1]
UniGeneiAt.31201.

Genome annotation databases

EnsemblPlantsiAT5G20320.1; AT5G20320.1; AT5G20320. [P84634-1]
GeneIDi832154.
KEGGiath:AT5G20320.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ118423 mRNA. Translation: AAZ80387.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92830.1.
RefSeqiNP_197532.3. NM_122039.4. [P84634-1]
UniGeneiAt.31201.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KOUNMR-A651-752[»]
ProteinModelPortaliP84634.
SMRiP84634. Positions 122-645, 651-752, 1103-1251, 1290-1530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17430. 4 interactions.
IntActiP84634. 2 interactions.
MINTiMINT-6948367.
STRINGi3702.AT5G20320.1.

PTM databases

iPTMnetiP84634.

Proteomic databases

PaxDbiP84634.
PRIDEiP84634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20320.1; AT5G20320.1; AT5G20320. [P84634-1]
GeneIDi832154.
KEGGiath:AT5G20320.

Organism-specific databases

TAIRiAT5G20320.

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
HOGENOMiHOG000083706.
InParanoidiP84634.
KOiK11592.
OMAiLDFWKCE.
PhylomeDBiP84634.

Enzyme and pathway databases

BioCyciARA:AT5G20320-MONOMER.
ARA:GQT-2073-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP84634.
PROiP84634.

Gene expression databases

ExpressionAtlasiP84634. baseline and differential.
GenevisibleiP84634. AT.

Family and domain databases

Gene3Di1.10.1520.10. 3 hits.
3.30.160.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 2 hits.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana."
    Xie Z., Allen E., Wilken A., Carrington J.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:12984-12989(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Columbia.
    Tissue: Shoot.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia1 Publication.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana."
    Hiraguri A., Itoh R., Kondo N., Nomura Y., Aizawa D., Murai Y., Koiwa H., Seki M., Shinozaki K., Fukuhara T.
    Plant Mol. Biol. 57:173-188(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DRB4.
  5. "Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans-acting siRNAs."
    Gasciolli V., Mallory A.C., Bartel D.P., Vaucheret H.
    Curr. Biol. 15:1494-1500(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis."
    Yoshikawa M., Peragine A., Park M.Y., Poethig R.S.
    Genes Dev. 19:2164-2175(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal."
    Dunoyer P., Himber C., Voinnet O.
    Nat. Genet. 37:1356-1360(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7."
    Adenot X., Elmayan T., Lauressergues D., Boutet S., Bouche N., Gasciolli V., Vaucheret H.
    Curr. Biol. 16:927-932(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs."
    Bouche N., Lauressergues D., Gasciolli V., Vaucheret H.
    EMBO J. 25:3347-3356(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "A novel class of bacteria-induced small RNAs in Arabidopsis."
    Katiyar-Agarwal S., Gao S., Vivian-Smith A., Jin H.
    Genes Dev. 21:3123-3134(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Suppression of antiviral silencing by cucumber mosaic virus 2b protein in Arabidopsis is associated with drastically reduced accumulation of three classes of viral small interfering RNAs."
    Diaz-Pendon J.A., Li F., Li W.X., Ding S.W.
    Plant Cell 19:2053-2063(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins."
    Moissiard G., Parizotto E.A., Himber C., Voinnet O.
    RNA 13:1268-1278(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Structural and genetic requirements for the biogenesis of tobacco rattle virus-derived small interfering RNAs."
    Donaire L., Barajas D., Martinez-Garcia B., Martinez-Priego L., Pagan I., Llave C.
    J. Virol. 82:5167-5177(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1."
    Qu F., Ye X., Morris T.J.
    Proc. Natl. Acad. Sci. U.S.A. 105:14732-14737(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Genome-wide comparative in silico analysis of the RNA helicase gene family in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza sativa."
    Xu R., Zhang S., Huang J., Zheng C.
    PLoS ONE 8:E78982-E78982(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  16. "Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction."
    Qin H., Chen F., Huan X., Machida S., Song J., Yuan Y.A.
    RNA 16:474-481(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 651-752.

Entry informationi

Entry nameiDCL4_ARATH
AccessioniPrimary (citable) accession number: P84634
Secondary accession number(s): Q3SA53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 8, 2011
Last modified: June 8, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.