P84625 (VHCA_ARCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-non-reducing hydrogenase iron-sulfur subunit A
EC=1.12.99.-

Gene names

Name:	mvhA
Ordered Locus Names:	Arcpr_1550

Organism

Archaeoglobus profundus (strain DSM 5631 / JCM 9629 / NBRC 100127 / Av18) [Complete proteome] [HAMAP]

Taxonomic identifier

572546 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a complex that provides reducing equivalents for heterodisulfide reductase. Ref.2
Cofactor	Nickel. Ref.2
Subunit structure	The F420-non-reducing hydrogenase is composed of three subunits; mvhA, mvhD and mvhG. It forms a complex with the heterodisulfide reductase (Hdr). Ref.2
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.
Sequence caution	The sequence ADB58596.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	F420-non-reducing hydrogenase iron-sulfur subunit A		PRO_0000199728
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P84625 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 3516D6C12C67F066	FASTA	486	54,452
	10 20 30 40 50 60 MKKIEIEPMT RLEGHGKIAI FLDDKGNVAD AFYQIVEFRG YETFLRGLPI EEVPRTVSTI 70 80 90 100 110 120 CGVCRAVHHT ASTKASDGVY GVEPTETAKK IRELYLNAHY VEDHCAILYA LGLPDFVVGI 130 140 150 160 170 180 EADPAERNLV GLIKKVGVDV GKTVLKKRFA AVKVLEMLGG KPIHPVAGLP GGWSKRITED 190 200 210 220 230 240 ERKEIEALAK EMVELGELTL QVFEDVILKN EKLLELITAD YYRVVVNYLG TVDDAGKVNY 250 260 270 280 290 300 YDGWQVGIDT KGNEVFRFKG KDYLNYIAER VVPWSYVKMP YFKQLGWKGF VDGEGTSLYS 310 320 330 340 350 360 VGPLARCNIG DMNTPKAKEA QEKMFDVLKE RPIHYIMAYH WARAVELLNA AERVLELAQD 370 380 390 400 410 420 PEITSPDVRA ELGEVTGEGV GIIEAPRGTL IHHYKTDENG IVTDANLIVA TTHNHAPINI 430 440 450 460 470 480 AIKKAAQHFI QENKEINDKL LNYVEIAYRP YDICQACASH ALPGRYPLEV LVYDSEGELI KTIRNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Archaeoglobus profundus type strain (AV18)." von Jan M., Lapidus A., Del Rio T.G., Copeland A., Tice H., Cheng J.F., Lucas S., Chen F., Nolan M., Goodwin L., Han C., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Ovchinnikova G., Chertkov O., Pati A. , Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Saunders E., Brettin T., Detter J.C., Chain P., Eichinger K., Huber H., Spring S., Rohde M., Goker M., Wirth R., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P. Stand. Genomic Sci. 2:327-346(2010) [PubMed: 21304717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5631 / JCM 9629 / NBRC 100127 / Av18.
[2]	"Two distinct heterodisulfide reductase-like enzymes in the sulfate-reducing archaeon Archaeoglobus profundus." Mander G.J., Pierik A.J., Huber H., Hedderich R. Eur. J. Biochem. 271:1106-1116(2004) [PubMed: 15009189] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20, COFACTOR, INTERACTION WITH HETERODISULFIDE REDUCTASE, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001857 Genomic DNA. Translation: ADB58596.1. Different initiation.
RefSeq	YP_003401269.1. NC_013741.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8740240.
GenomeReviews	Gene locus Arcpr_1550 in contig CP001857_GR.
KEGG	apo:Arcpr_1550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MEKAIQQ.
ProtClustDB	CLSK2803907.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. [Graphical view]
KO	K14126.
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. False negative. PS00508. NI_HGENASE_L_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHCA_ARCPA

Accession

Primary (citable) accession number: P84625
Secondary accession number(s): D2REQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	April 20, 2010
Last modified:	November 16, 2011
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program