Reviewed, UniProtKB/Swiss-Prot P84534 (ILV5_POPEU)

Names and origin

Protein names	Recommended name:     Ketol-acid reductoisomerase, chloroplastic     EC=1.1.1.86 Alternative name(s):     Acetohydroxy-acid reductoisomerase     Alpha-keto-beta-hydroxylacil reductoisomerase
Organism	Populus euphratica (Euphrates poplar)
Taxonomic identifier	75702 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	14 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. UniProtKB Q01292 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Cofactor	Magnesium By similarity. UniProtKB Q01292
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. UniProtKB Q01292 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. UniProtKB Q01292
Subunit structure	Tetramer of similar but non-identical chains By similarity. UniProtKB Q01292
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family. UniProtKB Q01292

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Ligand	Magnesium NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›14	›14	Ketol-acid reductoisomerase, chloroplastic		PRO_0000151266
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	14	1

Sequences

	Sequence		Length	Mass (Da)
	P84534-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 58F63F7536E61967	FASTA	14	1,614
	10 FYEKEGLPAF PMGK

References

[1]	"Proteome profiling of Populus euphratica Oliv. upon heat stress." Ferreira S., Hjernoe K., Larsen M., Wingsle G., Larsen P., Fey S., Roepstorff P., Pais M.S. Ann. Bot. 98:361-377(2006) [PubMed: 16740589] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Leaf.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.86. 293088.
Family and domain databases
InterPro	IPR013023. AcH_isomrdctse. [Graphical view]
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILV5_POPEU

Accession

Primary (citable) accession number: P84534

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)