Reviewed, UniProtKB/Swiss-Prot P84531 (RIP_CUCMO)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein cucurmosin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Cucurbita moschata (Cushaw squash) (Winter crookneck squash)
Taxonomic identifier	3662 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has cytotoxic activity towards cancer cells, but not normal cells. Inhibits the growth of the human leukemia cell line K562, the murine melanoma cell line B16 and the lung adenocarcinoma cell line A549 with IC50 values of 88.1 nM, 63.4 nM and 359.3 nM respectively. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Post-translational modification	The N-linked glycan consists of GlcNAc2Man3Xyl. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Ribosome-inactivating protein cucurmosin		PRO_0000221398
Sites
	Active site	70	1	Ref.1
	Active site	109	1	Ref.1
	Active site	158	1	Ref.1
	Active site	161	1	Ref.1
Amino acid modifications
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P84531-1 [UniParc]. Last modified December 15, 2009. Version 2. Checksum: EC055E4EA8AFB38C	FASTA	244	27,008
	10 20 30 40 50 60 NVRFDLSSAT SSSYKTFIKN LREALPKDGK VYDIPVLLST VMDSRRFILI DLVNYDGQSI 70 80 90 100 110 120 TAAIDVLNVY IVAYSTGTVS YFFQQVPAQA PKLLFKGTQQ RTLPYTGNYE NLQTAAKKLR 130 140 150 160 170 180 ENIELGLPAL DSAITTLFHY NAEAAASALL VLIQTTSEAA RFRYIELQIA NNVGTKFKPS 190 200 210 220 230 240 QTIISLENNW SALSKQIQIA KNKNGQFETP VILIDPQGNR VQITNVTSNV VTQNIQLLLN IGAT

References

[1]

"Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata." Hou X., Meehan E.J., Xie J., Huang M., Chen M., Chen L. J. Struct. Biol. 164:81-87(2008) [PubMed: 18652900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-236, PROTEIN SEQUENCE OF 1-27, X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, GLYCOSYLATION AT ASN-225, ACTIVE SITES. Tissue: Mesocarp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

EU309692 Genomic DNA. Translation: ABY47624.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BWH	X-ray	1.00	A	1-244	[»]

SMR

P84531. Positions 1-26.

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 66878.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIP_CUCMO

Accession

Primary (citable) accession number: P84531
Secondary accession number(s): B7SK17

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	December 15, 2009
Last modified:	February 9, 2010
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)