Reviewed, UniProtKB/Swiss-Prot P84531 (RIP_CUCMO)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein cucurmosin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Cucurbita moschata (Cushaw squash) (Winter crookneck squash)
Taxonomic identifier	3662 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	27 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily. UniProtKB P33185

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›27	›27	Ribosome-inactivating protein cucurmosin		PRO_0000221398
Experimental info
	Non-terminal residue	27	1

Sequences

	Sequence		Length	Mass (Da)
	P84531-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 2570A0CF3604B73E	FASTA	27	3,073
	10 20 NVRFDLSSAT SSSYKTFIKN LREALPK

References

[1]	"N-terminal amino acid sequences of cucurmosin, a RIP from the sarcocarp of Cucurbita moschata." Chen M.H. Submitted (APR-2005) to UniProtKB Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY. Tissue: Mesocarp.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 66878.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP_CUCMO

Accession

Primary (citable) accession number: P84531

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)