Skip Header

Contribute Send feedback
Read comments (?) or add your own

P84531 (RIP_CUCMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-inactivating protein cucurmosin
EC=3.2.2.22
Alternative name(s):
rRNA N-glycosidase
OrganismCucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata)
Taxonomic identifier3662 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbiteae › Cucurbita

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has cytotoxic activity towards cancer cells, but not normal cells. Inhibits the growth of the human leukemia cell line K562, the murine melanoma cell line B16 and the lung adenocarcinoma cell line A549 with IC50 values of 88.1 nM, 63.4 nM and 359.3 nM respectively. Ref.1

Catalytic activity

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1

Post-translational modification

The N-linked glycan consists of GlcNAc2Man3Xyl. Ref.1

Sequence similarities

Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
   Biological processPlant defense
   Molecular functionHydrolase
Protein synthesis inhibitor
Toxin
   PTMGlycoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processdefense response

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of translation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionrRNA N-glycosylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244Ribosome-inactivating protein cucurmosin
PRO_0000221398

Sites

Active site701 Ref.1
Active site1091 Ref.1
Active site1581 Ref.1
Active site1611 Ref.1

Amino acid modifications

Glycosylation1891N-linked (GlcNAc...) Potential
Glycosylation2251N-linked (GlcNAc...) Ref.1

Sequences

Sequence LengthMass (Da)Tools
P84531 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: EC055E4EA8AFB38C

FASTA24427,008
        10         20         30         40         50         60 
NVRFDLSSAT SSSYKTFIKN LREALPKDGK VYDIPVLLST VMDSRRFILI DLVNYDGQSI 

        70         80         90        100        110        120 
TAAIDVLNVY IVAYSTGTVS YFFQQVPAQA PKLLFKGTQQ RTLPYTGNYE NLQTAAKKLR 

       130        140        150        160        170        180 
ENIELGLPAL DSAITTLFHY NAEAAASALL VLIQTTSEAA RFRYIELQIA NNVGTKFKPS 

       190        200        210        220        230        240 
QTIISLENNW SALSKQIQIA KNKNGQFETP VILIDPQGNR VQITNVTSNV VTQNIQLLLN 


IGAT

« Hide

References

[1]"Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata."
Hou X., Meehan E.J., Xie J., Huang M., Chen M., Chen L.
J. Struct. Biol. 164:81-87(2008) [PubMed: 18652900] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-236, PROTEIN SEQUENCE OF 1-27, X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, GLYCOSYLATION AT ASN-225, ACTIVE SITES.
Tissue: Mesocarp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		EU309692 Genomic DNA. Translation: ABY47624.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BWHX-ray1.00A1-244[»]
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
Gene3DG3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
PfamPF00161. RIP. 1 hit.
[Graphical view]
PRINTSPR00396. SHIGARICIN.
SUPFAMSSF56371. Ribosome_inactivat_prot. 1 hit.
PROSITEPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIP_CUCMO
AccessionPrimary (citable) accession number: P84531
Secondary accession number(s): B7SK17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: December 15, 2009
Last modified: May 31, 2011
This is version 24 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents