Reviewed, UniProtKB/Swiss-Prot P84530 (RIP_LUFAC)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein luffaculin 1     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Luffa acutangula (Ridged gourd) (Sinkwa towelsponge)
Taxonomic identifier	56866 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Luffa

Protein attributes

Sequence length	10 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily. UniProtKB P33185

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›10	›10	Ribosome-inactivating protein luffaculin 1		PRO_0000221417
Experimental info
	Non-terminal residue	10	1

Sequences

	Sequence		Length	Mass (Da)
	P84530-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 987FEC65B735B9D5	FASTA	10	985
	10 DVSFSLSGSS

References

[1]	"N-terminal amino acid sequences of luffaculin 1, a RIP from the seeds of Luffa acutangula." Chen M.H. Submitted (APR-2005) to UniProtKB Cited for: PROTEIN SEQUENCE. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 314065.
Family and domain databases
InterPro	IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP_LUFAC

Accession

Primary (citable) accession number: P84530

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	May 24, 2005
Last modified:	March 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)