P84527 (KIWEL_ACTDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Kiwellin Alternative name(s): Allergen=Act d 5 Cleaved into the following 4 chains: Kissper KiTH-3 KiTH-1 KiTH-2
Organism	Actinidia deliciosa (Kiwi)
Taxonomic identifier	3627 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Actinidiaceae › Actinidia

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kissper is an anion-selective pore-forming peptide. Ref.2
Subcellular location	Secreted Potential.
Post-translational modification	Undergoes proteolytic cleavage by actinidin to produce kissper and KiTH. Three forms of KiTH are produced by cleavage at different sites, the main form produced in vivo is KiTH-1. Ref.3
Allergenic properties	Causes an allergic reaction in human. Binds to IgE from the serum of kiwi-allergic patients. Ref.1 Ref.3
Sequence similarities	Belongs to the kiwellin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Allergen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Kiwellin		PRO_0000083396
	Peptide	1 – 39	39	Kissper Ref.2		PRO_0000318767
	Chain	38 – 189	152	KiTH-3 Ref.3		PRO_0000341246
	Chain	40 – 189	150	KiTH-1 Ref.3		PRO_0000341247
	Chain	42 – 189	148	KiTH-2 Ref.3		PRO_0000341248
Natural variations
	Natural variant	61	1	Y → H. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P84527 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 731EC308E13E1104	FASTA	189	19,968
	10 20 30 40 50 60 ISSCNGPCRD LNDCDGQLIC IKGKCNDDPQ VGTHICRGTT HSHQPGGCKP SGTLTCRGKS 70 80 90 100 110 120 YPTYDCSPPV TSSTPAKLTN NDFSEGGDDG GPSECDESYH NNNERIVALS TGWYNGGSRC 130 140 150 160 170 180 GKMIRITASN GKSVSAKVVD ECDSRHGCDK EHAGQPPCRN NIVDGSNAVW SALGLDKNVG VVDITWSMA

References

[1]	"Kiwellin, a novel protein from kiwi fruit. Purification, biochemical characterization and identification as an allergen." Tamburrini M., Cerasuolo I., Carratore V., Stanziola A.A., Zofra S., Romano L., Camardella L., Ciardiello M.A. Protein J. 24:423-429(2005) [PubMed: 16328735] [Abstract] Cited for: PROTEIN SEQUENCE, ALLERGEN, VARIANT HIS-61. Tissue: Fruit.
[2]	"Kissper, a kiwi fruit peptide with channel-like activity: structural and functional features." Ciardiello M.A., Meleleo D., Saviano G., Crescenzo R., Carratore V., Camardella L., Gallucci E., Micelli S., Tancredi T., Picone D., Tamburrini M. J. Pept. Sci. 14:742-754(2008) [PubMed: 18186145] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-39, FUNCTION. Tissue: Fruit.
[3]	"Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding." Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A. J. Agric. Food Chem. 56:3812-3817(2008) [PubMed: 18442249] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-189, ALLERGEN, PROTEOLYTIC CLEAVAGE BY ACTINIDIN. Strain: cv. Hayward. Tissue: Fruit.

Cross-references

3D structure databases
ProteinModelPortal	P84527.
ModBase	Search...
Protein family/group databases
Allergome	2821. Act d 5. 3588. Act d 5.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014733. Barwin-like_endoglucanase. IPR009009. Barwin-related_endoglucanase. [Graphical view]
Gene3D	G3DSA:2.40.40.10. Barwin. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. [Graphical view]
SUPFAM	SSF50685. Barwin_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KIWEL_ACTDE

Accession

Primary (citable) accession number: P84527
Secondary accession number(s): P83975, P83976

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	January 11, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program