Reviewed, UniProtKB/Swiss-Prot P84469 (FERB_PARDE)

Names and origin

Protein names

Recommended name:
    Ferric reductase B
    EC=1.16.1.7

Gene names

Name:

ferB

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	27 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reductase activity that acts on Fe3+-chelates and uses both NADH and NADPH as electron donors. May play a role in iron uptake. Ref.1
Catalytic activity	2 Fe2+ + NAD+ = 2 Fe3+ + NADH. Ref.1
Cofactor	Binds 1 FAD per subunit. Ref.1
Subunit structure	Homodimer. Ref.1
Induction	By Fe3+ ion deprivation. Ref.1
biophysicochemical properties	Kinetic parameters: KM=0.4 mM for Fe3+EGTA and NADH KM=1.6 mM for Fe3+EDTA and NADH KM=0.4 mM for Fe3+citrate and NADH KM=0.4 mM for Fe3+chloride and NADH
Mass spectrometry	Molecular mass is 20196 Da from positions 1 - ?. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferric-chelate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›27	›27	Ferric reductase B		PRO_0000087228
Experimental info
	Non-terminal residue	27	1

Sequences

	Sequence		Length	Mass (Da)
	P84469-1 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 10D878742BCD3F4B	FASTA	27	3,012
	10 20 MVKTVAVMVG SLRKDSLAHK LMKVLQK

References

[1]

"Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans." Mazoch J., Tesarik R., Sedlacek V., Kucera I., Turanek J. Eur. J. Biochem. 271:553-562(2004) [PubMed: 14728682] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.16.1.7. 59.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

FERB_PARDE

Accession

Primary (citable) accession number: P84469

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	January 20, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)