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P84466

- ERG7_BOVIN

UniProt

P84466 - ERG7_BOVIN

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Protein

Lanosterol synthase

Gene

LSS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.1 Publication

Enzyme regulationi

Inhibited by the benzophenone containing OSC inhibitor Ro48-8071 and to a lesser extent by other benzophene containing inhibitors.1 Publication

Kineticsi

  1. KM=11 µM for (3S)-2,3-oxidosqualine1 Publication

pH dependencei

Optimum pH is 7.4. Active from pH 5.0 to 10.0.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius. Active from 25 to 50 degrees Celsius.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei232 – 2321Proton acceptorBy similarity
Active sitei455 – 4551Proton donorBy similarity

GO - Molecular functioni

  1. lanosterol synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Enzyme and pathway databases

SABIO-RKP84466.
UniPathwayiUPA00767; UER00753.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol synthase (EC:5.4.99.7)
Alternative name(s):
2,3-epoxysqualene--lanosterol cyclase
Oxidosqualene--lanosterol cyclase
Short name:
OSC
Gene namesi
Name:LSS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 732731Lanosterol synthasePRO_0000072658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP84466.

Expressioni

Tissue specificityi

Detected in the liver (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025201.

Structurei

3D structure databases

ProteinModelPortaliP84466.
SMRiP84466. Positions 6-732.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati124 – 16542PFTB 1Add
BLAST
Repeati483 – 52846PFTB 2Add
BLAST
Repeati560 – 60041PFTB 3Add
BLAST
Repeati612 – 66352PFTB 4Add
BLAST
Repeati670 – 71243PFTB 5Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.By similarity
Contains 5 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1657.
HOGENOMiHOG000234317.
HOVERGENiHBG005604.
InParanoidiP84466.
KOiK01852.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84466-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEGTCLRRR GGPYKTEPAT DLSRWRLSNQ VGRQTWTYSQ EEDPVREQSG
60 70 80 90 100
LEAHLLGLDT KSFFKDLPKA HTACRGALNG VTFYAALQTE DGHWAGDYGG
110 120 130 140 150
PLFLLPGLLI TCHVANIPLP AGYREEIIRY LRSVQLPDGG WGLHIEDKST
160 170 180 190 200
VFGTALNYVS LRILGVGPDD PDLVRARNLL HKKGGAVFIP SWGKFWLAVL
210 220 230 240 250
NVYSWEGLNT LFPEMWLFPD WMPAHPSTIW CHCRQVYLPM AYCYSTRLSA
260 270 280 290 300
EEGPLVQSLR QELYLEDYSC IDWAAHRNSV APDDLYTPHS WLLHVVYAIL
310 320 330 340 350
NLYERHHSTS LRQWATQKLY EHIAADDRFT KCISIGPISK TINMLVRWHV
360 370 380 390 400
DGPASAVFQE HVSRIPDYLW LGLDGMKMQG TNGSQIWDTA FAIQALLEAR
410 420 430 440 450
AQHRPEFWSC LRKAHEYLRI SQVPDNFPDY QKYYRHMSKG GFSFSTLDCG
460 470 480 490 500
WIVADCTAEA LKSILLLQEK CPFVSNHVPR ERLFDTVAVL LSLRNPDGGF
510 520 530 540 550
ATYETKRGGH LLELLNPSEV FGDIMIDYTY VECTSAVMQA LKTFHKQFPD
560 570 580 590 600
HRAGEIRETL EQGLQFCRQK QRPDGSWEGS WGVCFTYGAW FGLEAFACMG
610 620 630 640 650
HTYHNGVACA EISRACDFLL SRQMADGGWG EDFESCKQRR YVQSAQSQIH
660 670 680 690 700
NTCWALMGLM AVRHPDVAAL ERGVSYLLEK QLPNGDWPQE NISGVFNKSC
710 720 730
AISYTSYRNV FPIWTLGRFS RLHPDPALAG HP
Length:732
Mass (Da):83,184
Last modified:June 27, 2006 - v2
Checksum:iB06289C2FE03B4B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372933 mRNA. Translation: ABD24094.1.
RefSeqiNP_001040029.1. NM_001046564.1.
UniGeneiBt.46034.

Genome annotation databases

GeneIDi615906.
KEGGibta:615906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372933 mRNA. Translation: ABD24094.1 .
RefSeqi NP_001040029.1. NM_001046564.1.
UniGenei Bt.46034.

3D structure databases

ProteinModelPortali P84466.
SMRi P84466. Positions 6-732.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000025201.

Proteomic databases

PRIDEi P84466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 615906.
KEGGi bta:615906.

Organism-specific databases

CTDi 4047.

Phylogenomic databases

eggNOGi COG1657.
HOGENOMi HOG000234317.
HOVERGENi HBG005604.
InParanoidi P84466.
KOi K01852.

Enzyme and pathway databases

UniPathwayi UPA00767 ; UER00753 .
SABIO-RK P84466.

Miscellaneous databases

NextBioi 20899856.

Family and domain databases

Gene3Di 1.50.10.20. 2 hits.
InterProi IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00432. Prenyltrans. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 2 hits.
TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
PROSITEi PS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and active-site mapping of oxidosqualene-lanosterol cyclase from bovine liver."
    Chang C.-H., Huang C.-Y., Ko C.-Y., Wu T.-K.
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver."
    Wu T.-K., Huang C.-Y., Ko C.-Y., Chang C.-H., Chen Y.-J., Liao H.-K.
    Arch. Biochem. Biophys. 421:42-53(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 163-174; 247-258 AND 493-506, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, TISSUE SPECIFICITY.
    Tissue: Liver1 Publication.

Entry informationi

Entry nameiERG7_BOVIN
AccessioniPrimary (citable) accession number: P84466
Secondary accession number(s): Q2EMV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 27, 2006
Last modified: October 29, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3