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Protein

Lanosterol synthase

Gene

LSS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.1 Publication

Enzyme regulationi

Inhibited by the benzophenone containing OSC inhibitor Ro48-8071 and to a lesser extent by other benzophene containing inhibitors.1 Publication

Kineticsi

  1. KM=11 µM for (3S)-2,3-oxidosqualine1 Publication

    pH dependencei

    Optimum pH is 7.4. Active from pH 5.0 to 10.0.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius. Active from 25 to 50 degrees Celsius.1 Publication

    Pathway: lanosterol biosynthesis

    This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.1 Publication
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Squalene synthase (FDFT1)
    2. no protein annotated in this organism
    3. Lanosterol synthase (LSS)
    This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei232 – 2321Proton acceptorBy similarity
    Active sitei455 – 4551Proton donorBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Enzyme and pathway databases

    SABIO-RKP84466.
    UniPathwayiUPA00767; UER00753.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lanosterol synthase (EC:5.4.99.7)
    Alternative name(s):
    2,3-epoxysqualene--lanosterol cyclase
    Oxidosqualene--lanosterol cyclase
    Short name:
    OSC
    Gene namesi
    Name:LSS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136 Componenti: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 732731Lanosterol synthasePRO_0000072658Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP84466.

    Expressioni

    Tissue specificityi

    Detected in the liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025201.

    Structurei

    3D structure databases

    ProteinModelPortaliP84466.
    SMRiP84466. Positions 6-732.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati124 – 16542PFTB 1Add
    BLAST
    Repeati483 – 52846PFTB 2Add
    BLAST
    Repeati560 – 60041PFTB 3Add
    BLAST
    Repeati612 – 66352PFTB 4Add
    BLAST
    Repeati670 – 71243PFTB 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the terpene cyclase/mutase family.By similarity
    Contains 5 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1657.
    HOGENOMiHOG000234317.
    HOVERGENiHBG005604.
    InParanoidiP84466.
    KOiK01852.

    Family and domain databases

    Gene3Di1.50.10.20. 2 hits.
    InterProiIPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF00432. Prenyltrans. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
    PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P84466-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTEGTCLRRR GGPYKTEPAT DLSRWRLSNQ VGRQTWTYSQ EEDPVREQSG
    60 70 80 90 100
    LEAHLLGLDT KSFFKDLPKA HTACRGALNG VTFYAALQTE DGHWAGDYGG
    110 120 130 140 150
    PLFLLPGLLI TCHVANIPLP AGYREEIIRY LRSVQLPDGG WGLHIEDKST
    160 170 180 190 200
    VFGTALNYVS LRILGVGPDD PDLVRARNLL HKKGGAVFIP SWGKFWLAVL
    210 220 230 240 250
    NVYSWEGLNT LFPEMWLFPD WMPAHPSTIW CHCRQVYLPM AYCYSTRLSA
    260 270 280 290 300
    EEGPLVQSLR QELYLEDYSC IDWAAHRNSV APDDLYTPHS WLLHVVYAIL
    310 320 330 340 350
    NLYERHHSTS LRQWATQKLY EHIAADDRFT KCISIGPISK TINMLVRWHV
    360 370 380 390 400
    DGPASAVFQE HVSRIPDYLW LGLDGMKMQG TNGSQIWDTA FAIQALLEAR
    410 420 430 440 450
    AQHRPEFWSC LRKAHEYLRI SQVPDNFPDY QKYYRHMSKG GFSFSTLDCG
    460 470 480 490 500
    WIVADCTAEA LKSILLLQEK CPFVSNHVPR ERLFDTVAVL LSLRNPDGGF
    510 520 530 540 550
    ATYETKRGGH LLELLNPSEV FGDIMIDYTY VECTSAVMQA LKTFHKQFPD
    560 570 580 590 600
    HRAGEIRETL EQGLQFCRQK QRPDGSWEGS WGVCFTYGAW FGLEAFACMG
    610 620 630 640 650
    HTYHNGVACA EISRACDFLL SRQMADGGWG EDFESCKQRR YVQSAQSQIH
    660 670 680 690 700
    NTCWALMGLM AVRHPDVAAL ERGVSYLLEK QLPNGDWPQE NISGVFNKSC
    710 720 730
    AISYTSYRNV FPIWTLGRFS RLHPDPALAG HP
    Length:732
    Mass (Da):83,184
    Last modified:June 27, 2006 - v2
    Checksum:iB06289C2FE03B4B2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ372933 mRNA. Translation: ABD24094.1.
    RefSeqiNP_001040029.1. NM_001046564.1.
    UniGeneiBt.46034.

    Genome annotation databases

    GeneIDi615906.
    KEGGibta:615906.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ372933 mRNA. Translation: ABD24094.1.
    RefSeqiNP_001040029.1. NM_001046564.1.
    UniGeneiBt.46034.

    3D structure databases

    ProteinModelPortaliP84466.
    SMRiP84466. Positions 6-732.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025201.

    Proteomic databases

    PRIDEiP84466.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi615906.
    KEGGibta:615906.

    Organism-specific databases

    CTDi4047.

    Phylogenomic databases

    eggNOGiCOG1657.
    HOGENOMiHOG000234317.
    HOVERGENiHBG005604.
    InParanoidiP84466.
    KOiK01852.

    Enzyme and pathway databases

    UniPathwayiUPA00767; UER00753.
    SABIO-RKP84466.

    Miscellaneous databases

    NextBioi20899856.

    Family and domain databases

    Gene3Di1.50.10.20. 2 hits.
    InterProiIPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF00432. Prenyltrans. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
    PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular cloning and active-site mapping of oxidosqualene-lanosterol cyclase from bovine liver."
      Chang C.-H., Huang C.-Y., Ko C.-Y., Wu T.-K.
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver."
      Wu T.-K., Huang C.-Y., Ko C.-Y., Chang C.-H., Chen Y.-J., Liao H.-K.
      Arch. Biochem. Biophys. 421:42-53(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 163-174; 247-258 AND 493-506, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, TISSUE SPECIFICITY.
      Tissue: Liver1 Publication.

    Entry informationi

    Entry nameiERG7_BOVIN
    AccessioniPrimary (citable) accession number: P84466
    Secondary accession number(s): Q2EMV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: June 27, 2006
    Last modified: March 4, 2015
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.