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P84466

- ERG7_BOVIN

UniProt

P84466 - ERG7_BOVIN

Protein

Lanosterol synthase

Gene

LSS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.1 Publication

    Catalytic activityi

    (3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.1 Publication

    Enzyme regulationi

    Inhibited by the benzophenone containing OSC inhibitor Ro48-8071 and to a lesser extent by other benzophene containing inhibitors.1 Publication

    Kineticsi

    1. KM=11 µM for (3S)-2,3-oxidosqualine1 Publication

    pH dependencei

    Optimum pH is 7.4. Active from pH 5.0 to 10.0.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius. Active from 25 to 50 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei232 – 2321Proton acceptorBy similarity
    Active sitei455 – 4551Proton donorBy similarity

    GO - Molecular functioni

    1. lanosterol synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Enzyme and pathway databases

    SABIO-RKP84466.
    UniPathwayiUPA00767; UER00753.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lanosterol synthase (EC:5.4.99.7)
    Alternative name(s):
    2,3-epoxysqualene--lanosterol cyclase
    Oxidosqualene--lanosterol cyclase
    Short name:
    OSC
    Gene namesi
    Name:LSS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 732731Lanosterol synthasePRO_0000072658Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP84466.

    Expressioni

    Tissue specificityi

    Detected in the liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025201.

    Structurei

    3D structure databases

    ProteinModelPortaliP84466.
    SMRiP84466. Positions 6-732.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati124 – 16542PFTB 1Add
    BLAST
    Repeati483 – 52846PFTB 2Add
    BLAST
    Repeati560 – 60041PFTB 3Add
    BLAST
    Repeati612 – 66352PFTB 4Add
    BLAST
    Repeati670 – 71243PFTB 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the terpene cyclase/mutase family.By similarity
    Contains 5 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1657.
    HOGENOMiHOG000234317.
    HOVERGENiHBG005604.
    InParanoidiP84466.
    KOiK01852.

    Family and domain databases

    Gene3Di1.50.10.20. 2 hits.
    InterProiIPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF00432. Prenyltrans. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
    PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P84466-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEGTCLRRR GGPYKTEPAT DLSRWRLSNQ VGRQTWTYSQ EEDPVREQSG    50
    LEAHLLGLDT KSFFKDLPKA HTACRGALNG VTFYAALQTE DGHWAGDYGG 100
    PLFLLPGLLI TCHVANIPLP AGYREEIIRY LRSVQLPDGG WGLHIEDKST 150
    VFGTALNYVS LRILGVGPDD PDLVRARNLL HKKGGAVFIP SWGKFWLAVL 200
    NVYSWEGLNT LFPEMWLFPD WMPAHPSTIW CHCRQVYLPM AYCYSTRLSA 250
    EEGPLVQSLR QELYLEDYSC IDWAAHRNSV APDDLYTPHS WLLHVVYAIL 300
    NLYERHHSTS LRQWATQKLY EHIAADDRFT KCISIGPISK TINMLVRWHV 350
    DGPASAVFQE HVSRIPDYLW LGLDGMKMQG TNGSQIWDTA FAIQALLEAR 400
    AQHRPEFWSC LRKAHEYLRI SQVPDNFPDY QKYYRHMSKG GFSFSTLDCG 450
    WIVADCTAEA LKSILLLQEK CPFVSNHVPR ERLFDTVAVL LSLRNPDGGF 500
    ATYETKRGGH LLELLNPSEV FGDIMIDYTY VECTSAVMQA LKTFHKQFPD 550
    HRAGEIRETL EQGLQFCRQK QRPDGSWEGS WGVCFTYGAW FGLEAFACMG 600
    HTYHNGVACA EISRACDFLL SRQMADGGWG EDFESCKQRR YVQSAQSQIH 650
    NTCWALMGLM AVRHPDVAAL ERGVSYLLEK QLPNGDWPQE NISGVFNKSC 700
    AISYTSYRNV FPIWTLGRFS RLHPDPALAG HP 732
    Length:732
    Mass (Da):83,184
    Last modified:June 27, 2006 - v2
    Checksum:iB06289C2FE03B4B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ372933 mRNA. Translation: ABD24094.1.
    RefSeqiNP_001040029.1. NM_001046564.1.
    UniGeneiBt.46034.

    Genome annotation databases

    GeneIDi615906.
    KEGGibta:615906.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ372933 mRNA. Translation: ABD24094.1 .
    RefSeqi NP_001040029.1. NM_001046564.1.
    UniGenei Bt.46034.

    3D structure databases

    ProteinModelPortali P84466.
    SMRi P84466. Positions 6-732.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000025201.

    Proteomic databases

    PRIDEi P84466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 615906.
    KEGGi bta:615906.

    Organism-specific databases

    CTDi 4047.

    Phylogenomic databases

    eggNOGi COG1657.
    HOGENOMi HOG000234317.
    HOVERGENi HBG005604.
    InParanoidi P84466.
    KOi K01852.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00753 .
    SABIO-RK P84466.

    Miscellaneous databases

    NextBioi 20899856.

    Family and domain databases

    Gene3Di 1.50.10.20. 2 hits.
    InterProi IPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR002365. Terpene_synthase_CS.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view ]
    Pfami PF00432. Prenyltrans. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 2 hits.
    TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
    PROSITEi PS01074. TERPENE_SYNTHASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and active-site mapping of oxidosqualene-lanosterol cyclase from bovine liver."
      Chang C.-H., Huang C.-Y., Ko C.-Y., Wu T.-K.
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver."
      Wu T.-K., Huang C.-Y., Ko C.-Y., Chang C.-H., Chen Y.-J., Liao H.-K.
      Arch. Biochem. Biophys. 421:42-53(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 163-174; 247-258 AND 493-506, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, TISSUE SPECIFICITY.
      Tissue: Liver1 Publication.

    Entry informationi

    Entry nameiERG7_BOVIN
    AccessioniPrimary (citable) accession number: P84466
    Secondary accession number(s): Q2EMV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3