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Protein

C-C motif chemokine 21a

Gene

Ccl21a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.

GO - Molecular functioni

  1. CCR7 chemokine receptor binding Source: BHF-UCL
  2. chemokine activity Source: BHF-UCL

GO - Biological processi

  1. activation of GTPase activity Source: BHF-UCL
  2. cell maturation Source: BHF-UCL
  3. dendritic cell chemotaxis Source: BHF-UCL
  4. dendritic cell dendrite assembly Source: BHF-UCL
  5. establishment of T cell polarity Source: BHF-UCL
  6. immune response Source: InterPro
  7. immunological synapse formation Source: BHF-UCL
  8. inflammatory response Source: UniProtKB-KW
  9. mesangial cell-matrix adhesion Source: BHF-UCL
  10. negative regulation of dendritic cell dendrite assembly Source: BHF-UCL
  11. negative regulation of leukocyte apoptotic process Source: BHF-UCL
  12. positive regulation of actin filament polymerization Source: BHF-UCL
  13. positive regulation of cell adhesion mediated by integrin Source: BHF-UCL
  14. positive regulation of cell-matrix adhesion Source: BHF-UCL
  15. positive regulation of cell motility Source: BHF-UCL
  16. positive regulation of chemotaxis Source: BHF-UCL
  17. positive regulation of dendritic cell antigen processing and presentation Source: BHF-UCL
  18. positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  19. positive regulation of filopodium assembly Source: BHF-UCL
  20. positive regulation of glycoprotein biosynthetic process Source: BHF-UCL
  21. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: BHF-UCL
  22. positive regulation of JNK cascade Source: BHF-UCL
  23. positive regulation of macrophage chemotaxis Source: BHF-UCL
  24. positive regulation of myeloid dendritic cell chemotaxis Source: BHF-UCL
  25. positive regulation of neutrophil chemotaxis Source: BHF-UCL
  26. positive regulation of phosphatidylinositol 3-kinase activity Source: BHF-UCL
  27. positive regulation of protein kinase activity Source: BHF-UCL
  28. positive regulation of protein kinase B signaling Source: BHF-UCL
  29. positive regulation of pseudopodium assembly Source: BHF-UCL
  30. positive regulation of receptor-mediated endocytosis Source: BHF-UCL
  31. release of sequestered calcium ion into cytosol Source: BHF-UCL
  32. response to prostaglandin E Source: BHF-UCL
  33. ruffle organization Source: BHF-UCL
  34. T cell costimulation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_288802. Chemokine receptors bind chemokines.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 21a
Alternative name(s):
6Ckine
Beta-chemokine exodus-2
Small-inducible cytokine A21a
Thymus-derived chemotactic agent 4
Short name:
TCA4
Gene namesi
Name:Ccl21a
Synonyms:Scya21, Scya21a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componentsi: Chromosome 4, Unplaced

Organism-specific databases

MGIiMGI:1349183. Ccl21a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Involvement in diseasei

Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 133110C-C motif chemokine 21aPRO_0000005223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 57By similarity
Disulfide bondi32 ↔ 75By similarity
Disulfide bondi103 ↔ 122Sequence Analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP84444.
PRIDEiP84444.

Expressioni

Tissue specificityi

Expressed strongly in lung, spleen, thymus, peripheral and mesentric lymph nodes. Also expressed in the testis, kidney, liver, and heart.

Gene expression databases

BgeeiP84444.
CleanExiMM_CCL21B.
GenevestigatoriP84444.

Interactioni

Subunit structurei

Binds to CCR7 and to CXCR3.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103647.

Structurei

3D structure databases

ProteinModelPortaliP84444.
SMRiP84444. Positions 24-92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 13336C-terminal basic extensionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46090.
GeneTreeiENSGT00730000111297.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP84444.
KOiK16062.
OMAiCCLKYSL.
OrthoDBiEOG7GXPDG.
PhylomeDBiP84444.
TreeFamiTF338224.

Family and domain databases

InterProiIPR030593. CCL21.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR12015:SF7. PTHR12015:SF7. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQMMTLSLL SLVLALCIPW TQGSDGGGQD CCLKYSQKKI PYSIVRGYRK
60 70 80 90 100
QEPSLGCPIP AILFSPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS
110 120 130
PGCRKNRGTS KSGKKGKGSK GCKRTEQTQP SRG
Length:133
Mass (Da):14,558
Last modified:March 1, 2005 - v1
Checksum:iABD2750A320B3719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001980 mRNA. Translation: AAB86595.1.
AF006637 mRNA. Translation: AAB61440.1.
AF171086 Genomic DNA. Translation: AAF16901.1.
AF035684 Genomic DNA. Translation: AAC82613.1.
AF307985 Genomic DNA. Translation: AAG45833.1.
AK144258 mRNA. Translation: BAE25802.1.
AK155924 mRNA. Translation: BAE33505.1.
AL772334 Genomic DNA. No translation available.
BC025974 mRNA. Translation: AAH25974.1.
BC028747 mRNA. Translation: AAH28747.1.
BC038120 mRNA. Translation: AAH38120.1.
CCDSiCCDS18081.1.
RefSeqiNP_001180596.1. NM_001193667.1.
NP_035254.1. NM_011124.4.
UniGeneiMm.220853.
Mm.458815.

Genome annotation databases

EnsembliENSMUST00000095114; ENSMUSP00000092732; ENSMUSG00000094686.
ENSMUST00000178919; ENSMUSP00000136780; ENSMUSG00000095320.
GeneIDi100504362.
18829.
KEGGimmu:100504362.
mmu:18829.
UCSCiuc008sny.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001980 mRNA. Translation: AAB86595.1.
AF006637 mRNA. Translation: AAB61440.1.
AF171086 Genomic DNA. Translation: AAF16901.1.
AF035684 Genomic DNA. Translation: AAC82613.1.
AF307985 Genomic DNA. Translation: AAG45833.1.
AK144258 mRNA. Translation: BAE25802.1.
AK155924 mRNA. Translation: BAE33505.1.
AL772334 Genomic DNA. No translation available.
BC025974 mRNA. Translation: AAH25974.1.
BC028747 mRNA. Translation: AAH28747.1.
BC038120 mRNA. Translation: AAH38120.1.
CCDSiCCDS18081.1.
RefSeqiNP_001180596.1. NM_001193667.1.
NP_035254.1. NM_011124.4.
UniGeneiMm.220853.
Mm.458815.

3D structure databases

ProteinModelPortaliP84444.
SMRiP84444. Positions 24-92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103647.

Proteomic databases

PaxDbiP84444.
PRIDEiP84444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095114; ENSMUSP00000092732; ENSMUSG00000094686.
ENSMUST00000178919; ENSMUSP00000136780; ENSMUSG00000095320.
GeneIDi100504362.
18829.
KEGGimmu:100504362.
mmu:18829.
UCSCiuc008sny.2. mouse.

Organism-specific databases

CTDi18829.
MGIiMGI:1349183. Ccl21a.

Phylogenomic databases

eggNOGiNOG46090.
GeneTreeiENSGT00730000111297.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP84444.
KOiK16062.
OMAiCCLKYSL.
OrthoDBiEOG7GXPDG.
PhylomeDBiP84444.
TreeFamiTF338224.

Enzyme and pathway databases

ReactomeiREACT_288802. Chemokine receptors bind chemokines.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi295204.
PROiP84444.
SOURCEiSearch...

Gene expression databases

BgeeiP84444.
CleanExiMM_CCL21B.
GenevestigatoriP84444.

Family and domain databases

InterProiIPR030593. CCL21.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR12015:SF7. PTHR12015:SF7. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel beta chemokine containing six conserved cysteines."
    Hedrick J.A., Zlotnik A.
    J. Immunol. 159:1589-1593(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of a new mouse beta-chemokine, thymus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells."
    Tanabe S., Lu Z., Luo Y., Quackenbush E.J., Berman M.A., Collins-Racie L.A., Mi S., Reilly C., Lo D., Jacobs K.A., Dorf M.E.
    J. Immunol. 159:5671-5679(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c and C57BL/6J.
    Tissue: Thymus.
  3. "The reduced expression of 6Ckine in the plt mouse results from the deletion of one of two 6Ckine genes."
    Vassileva G., Soto H., Zlotnik A., Nakano H., Kakiuchi T., Hedrick J.A., Lira S.A.
    J. Exp. Med. 190:1183-1188(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Embryonic stem cell.
  4. "Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation."
    Nakano H., Gunn M.D.
    J. Immunol. 166:361-369(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Ola.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Lymph node and Spleen.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Colon and Mammary gland.
  8. "A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes."
    Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D., Williams L.T.
    Proc. Natl. Acad. Sci. U.S.A. 95:258-263(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCC21A_MOUSE
AccessioniPrimary (citable) accession number: P84444
Secondary accession number(s): O09002
, O09006, Q3U1J2, Q91V84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 29, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Three genes code for Ccl21 in mouse. Ccl21b and Ccl21c produce identical proteins while the protein produced by Ccl21a differs at only one position. Ccl21b and Ccl21c have 'Leu-65' (6Ckine-Leu) while Ccl21a has Ser-65 (6Ckine-Ser).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.