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P84444

- CC21A_MOUSE

UniProt

P84444 - CC21A_MOUSE

Protein

C-C motif chemokine 21a

Gene

Ccl21a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.

    GO - Molecular functioni

    1. CCR7 chemokine receptor binding Source: BHF-UCL
    2. chemokine activity Source: BHF-UCL

    GO - Biological processi

    1. activation of Rho GTPase activity Source: BHF-UCL
    2. cell maturation Source: BHF-UCL
    3. dendritic cell chemotaxis Source: BHF-UCL
    4. dendritic cell dendrite assembly Source: BHF-UCL
    5. establishment of T cell polarity Source: BHF-UCL
    6. immune response Source: InterPro
    7. immunological synapse formation Source: BHF-UCL
    8. inflammatory response Source: UniProtKB-KW
    9. mesangial cell-matrix adhesion Source: BHF-UCL
    10. negative regulation of dendritic cell dendrite assembly Source: BHF-UCL
    11. negative regulation of leukocyte apoptotic process Source: BHF-UCL
    12. positive regulation of actin filament polymerization Source: BHF-UCL
    13. positive regulation of cell adhesion mediated by integrin Source: BHF-UCL
    14. positive regulation of cell-matrix adhesion Source: BHF-UCL
    15. positive regulation of cell motility Source: BHF-UCL
    16. positive regulation of chemotaxis Source: BHF-UCL
    17. positive regulation of dendritic cell antigen processing and presentation Source: BHF-UCL
    18. positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
    19. positive regulation of filopodium assembly Source: BHF-UCL
    20. positive regulation of glycoprotein biosynthetic process Source: BHF-UCL
    21. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: BHF-UCL
    22. positive regulation of JNK cascade Source: BHF-UCL
    23. positive regulation of macrophage chemotaxis Source: BHF-UCL
    24. positive regulation of myeloid dendritic cell chemotaxis Source: BHF-UCL
    25. positive regulation of neutrophil chemotaxis Source: BHF-UCL
    26. positive regulation of phosphatidylinositol 3-kinase activity Source: BHF-UCL
    27. positive regulation of protein kinase activity Source: BHF-UCL
    28. positive regulation of protein kinase B signaling Source: BHF-UCL
    29. positive regulation of pseudopodium assembly Source: BHF-UCL
    30. positive regulation of receptor-mediated endocytosis Source: BHF-UCL
    31. release of sequestered calcium ion into cytosol Source: BHF-UCL
    32. response to prostaglandin E Source: BHF-UCL
    33. ruffle organization Source: BHF-UCL
    34. T cell costimulation Source: BHF-UCL

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 21a
    Alternative name(s):
    6Ckine
    Beta-chemokine exodus-2
    Small-inducible cytokine A21a
    Thymus-derived chemotactic agent 4
    Short name:
    TCA4
    Gene namesi
    Name:Ccl21a
    Synonyms:Scya21, Scya21a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1349183. Ccl21a.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 133110C-C motif chemokine 21aPRO_0000005223Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 57By similarity
    Disulfide bondi32 ↔ 75By similarity
    Disulfide bondi103 ↔ 122Sequence Analysis

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP84444.
    PRIDEiP84444.

    Expressioni

    Tissue specificityi

    Expressed strongly in lung, spleen, thymus, peripheral and mesentric lymph nodes. Also expressed in the testis, kidney, liver, and heart.

    Gene expression databases

    BgeeiP84444.
    CleanExiMM_CCL21B.
    GenevestigatoriP84444.

    Interactioni

    Subunit structurei

    Binds to CCR7 and to CXCR3.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000103647.

    Structurei

    3D structure databases

    ProteinModelPortaliP84444.
    SMRiP84444. Positions 24-92.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni98 – 13336C-terminal basic extensionAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG46090.
    GeneTreeiENSGT00730000111297.
    HOGENOMiHOG000036685.
    HOVERGENiHBG017871.
    InParanoidiP84444.
    KOiK16062.
    OMAiCCLKYSL.
    OrthoDBiEOG7GXPDG.
    PhylomeDBiP84444.
    TreeFamiTF338224.

    Family and domain databases

    InterProiIPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P84444-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQMMTLSLL SLVLALCIPW TQGSDGGGQD CCLKYSQKKI PYSIVRGYRK    50
    QEPSLGCPIP AILFSPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS 100
    PGCRKNRGTS KSGKKGKGSK GCKRTEQTQP SRG 133
    Length:133
    Mass (Da):14,558
    Last modified:March 1, 2005 - v1
    Checksum:iABD2750A320B3719
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001980 mRNA. Translation: AAB86595.1.
    AF006637 mRNA. Translation: AAB61440.1.
    AF171086 Genomic DNA. Translation: AAF16901.1.
    AF035684 Genomic DNA. Translation: AAC82613.1.
    AF307985 Genomic DNA. Translation: AAG45833.1.
    AK144258 mRNA. Translation: BAE25802.1.
    AK155924 mRNA. Translation: BAE33505.1.
    AL772334 Genomic DNA. No translation available.
    BC025974 mRNA. Translation: AAH25974.1.
    BC028747 mRNA. Translation: AAH28747.1.
    BC038120 mRNA. Translation: AAH38120.1.
    CCDSiCCDS18081.1.
    RefSeqiNP_001180596.1. NM_001193667.1.
    NP_035254.1. NM_011124.4.
    UniGeneiMm.220853.
    Mm.458815.

    Genome annotation databases

    EnsembliENSMUST00000095114; ENSMUSP00000092732; ENSMUSG00000094686.
    ENSMUST00000178919; ENSMUSP00000136780; ENSMUSG00000095320.
    GeneIDi100504362.
    18829.
    KEGGimmu:100504362.
    mmu:18829.
    UCSCiuc008sny.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001980 mRNA. Translation: AAB86595.1 .
    AF006637 mRNA. Translation: AAB61440.1 .
    AF171086 Genomic DNA. Translation: AAF16901.1 .
    AF035684 Genomic DNA. Translation: AAC82613.1 .
    AF307985 Genomic DNA. Translation: AAG45833.1 .
    AK144258 mRNA. Translation: BAE25802.1 .
    AK155924 mRNA. Translation: BAE33505.1 .
    AL772334 Genomic DNA. No translation available.
    BC025974 mRNA. Translation: AAH25974.1 .
    BC028747 mRNA. Translation: AAH28747.1 .
    BC038120 mRNA. Translation: AAH38120.1 .
    CCDSi CCDS18081.1.
    RefSeqi NP_001180596.1. NM_001193667.1.
    NP_035254.1. NM_011124.4.
    UniGenei Mm.220853.
    Mm.458815.

    3D structure databases

    ProteinModelPortali P84444.
    SMRi P84444. Positions 24-92.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000103647.

    Proteomic databases

    PaxDbi P84444.
    PRIDEi P84444.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000095114 ; ENSMUSP00000092732 ; ENSMUSG00000094686 .
    ENSMUST00000178919 ; ENSMUSP00000136780 ; ENSMUSG00000095320 .
    GeneIDi 100504362.
    18829.
    KEGGi mmu:100504362.
    mmu:18829.
    UCSCi uc008sny.2. mouse.

    Organism-specific databases

    CTDi 18829.
    MGIi MGI:1349183. Ccl21a.

    Phylogenomic databases

    eggNOGi NOG46090.
    GeneTreei ENSGT00730000111297.
    HOGENOMi HOG000036685.
    HOVERGENi HBG017871.
    InParanoidi P84444.
    KOi K16062.
    OMAi CCLKYSL.
    OrthoDBi EOG7GXPDG.
    PhylomeDBi P84444.
    TreeFami TF338224.

    Miscellaneous databases

    NextBioi 295204.
    PROi P84444.
    SOURCEi Search...

    Gene expression databases

    Bgeei P84444.
    CleanExi MM_CCL21B.
    Genevestigatori P84444.

    Family and domain databases

    InterProi IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel beta chemokine containing six conserved cysteines."
      Hedrick J.A., Zlotnik A.
      J. Immunol. 159:1589-1593(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification of a new mouse beta-chemokine, thymus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells."
      Tanabe S., Lu Z., Luo Y., Quackenbush E.J., Berman M.A., Collins-Racie L.A., Mi S., Reilly C., Lo D., Jacobs K.A., Dorf M.E.
      J. Immunol. 159:5671-5679(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c and C57BL/6J.
      Tissue: Thymus.
    3. "The reduced expression of 6Ckine in the plt mouse results from the deletion of one of two 6Ckine genes."
      Vassileva G., Soto H., Zlotnik A., Nakano H., Kakiuchi T., Hedrick J.A., Lira S.A.
      J. Exp. Med. 190:1183-1188(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
      Tissue: Embryonic stem cell.
    4. "Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation."
      Nakano H., Gunn M.D.
      J. Immunol. 166:361-369(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Lymph node and Spleen.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Colon and Mammary gland.
    8. "A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes."
      Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D., Williams L.T.
      Proc. Natl. Acad. Sci. U.S.A. 95:258-263(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiCC21A_MOUSE
    AccessioniPrimary (citable) accession number: P84444
    Secondary accession number(s): O09002
    , O09006, Q3U1J2, Q91V84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Three genes code for Ccl21 in mouse. Ccl21b and Ccl21c produce identical proteins while the protein produced by Ccl21a differs at only one position. Ccl21b and Ccl21c have 'Leu-65' (6Ckine-Leu) while Ccl21a has Ser-65 (6Ckine-Ser).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3