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P84444 (CC21A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 21a
Alternative name(s):
6Ckine
Beta-chemokine exodus-2
Small-inducible cytokine A21a
Thymus-derived chemotactic agent 4
Short name=TCA4
Gene names
Name:Ccl21a
Synonyms:Scya21, Scya21a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length133 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.

Subunit structure

Binds to CCR7 and to CXCR3.

Subcellular location

Secreted.

Tissue specificity

Expressed strongly in lung, spleen, thymus, peripheral and mesentric lymph nodes. Also expressed in the testis, kidney, liver, and heart.

Involvement in disease

Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b.

Miscellaneous

Three genes code for Ccl21 in mouse. Ccl21b and Ccl21c produce identical proteins while the protein produced by Ccl21a differs at only one position. Ccl21b and Ccl21c have 'Leu-65' (6Ckine-Leu) while Ccl21a has Ser-65 (6Ckine-Ser).

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
   Biological processChemotaxis
Inflammatory response
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell costimulation

Inferred from direct assay PubMed 15845453. Source: BHF-UCL

activation of Rho GTPase activity

Inferred from sequence or structural similarity. Source: BHF-UCL

cell maturation

Inferred from direct assay PubMed 15845453. Source: BHF-UCL

dendritic cell chemotaxis

Inferred from direct assay PubMed 12200351. Source: BHF-UCL

dendritic cell dendrite assembly

Inferred from direct assay PubMed 12200351. Source: BHF-UCL

establishment of T cell polarity

Inferred from sequence or structural similarity. Source: BHF-UCL

immune response

Inferred from electronic annotation. Source: InterPro

immunological synapse formation

Inferred from direct assay PubMed 15845453. Source: BHF-UCL

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

mesangial cell-matrix adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of dendritic cell dendrite assembly

Inferred from direct assay PubMed 12200351. Source: BHF-UCL

negative regulation of leukocyte apoptotic process

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of JNK cascade

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of actin filament polymerization

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell adhesion mediated by integrin

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell motility

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell-matrix adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of chemotaxis

Inferred from direct assay PubMed 9507024. Source: BHF-UCL

positive regulation of dendritic cell antigen processing and presentation

Inferred from direct assay PubMed 15845453. Source: BHF-UCL

positive regulation of filopodium assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of glycoprotein biosynthetic process

Inferred from direct assay PubMed 15845453. Source: BHF-UCL

positive regulation of macrophage chemotaxis

Non-traceable author statement PubMed 20605225. Source: BHF-UCL

positive regulation of myeloid dendritic cell chemotaxis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of neutrophil chemotaxis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of phosphatidylinositol 3-kinase activity

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of protein kinase B signaling

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of protein kinase activity

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of pseudopodium assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of receptor-mediated endocytosis

Inferred from direct assay PubMed 12609829. Source: BHF-UCL

release of sequestered calcium ion into cytosol

Inferred from sequence or structural similarity. Source: BHF-UCL

response to prostaglandin E

Inferred from sequence or structural similarity. Source: BHF-UCL

ruffle organization

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionCCR7 chemokine receptor binding

Inferred from physical interaction PubMed 9507024. Source: BHF-UCL

chemokine activity

Inferred from direct assay PubMed 12200351. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 133110C-C motif chemokine 21a
PRO_0000005223

Regions

Region98 – 13336C-terminal basic extension

Amino acid modifications

Disulfide bond31 ↔ 57 By similarity
Disulfide bond32 ↔ 75 By similarity
Disulfide bond103 ↔ 122 Potential

Sequences

Sequence LengthMass (Da)Tools
P84444 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: ABD2750A320B3719

FASTA13314,558
        10         20         30         40         50         60 
MAQMMTLSLL SLVLALCIPW TQGSDGGGQD CCLKYSQKKI PYSIVRGYRK QEPSLGCPIP 

        70         80         90        100        110        120 
AILFSPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS PGCRKNRGTS KSGKKGKGSK 

       130 
GCKRTEQTQP SRG 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a novel beta chemokine containing six conserved cysteines."
Hedrick J.A., Zlotnik A.
J. Immunol. 159:1589-1593(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of a new mouse beta-chemokine, thymus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells."
Tanabe S., Lu Z., Luo Y., Quackenbush E.J., Berman M.A., Collins-Racie L.A., Mi S., Reilly C., Lo D., Jacobs K.A., Dorf M.E.
J. Immunol. 159:5671-5679(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c and C57BL/6J.
Tissue: Thymus.
[3]"The reduced expression of 6Ckine in the plt mouse results from the deletion of one of two 6Ckine genes."
Vassileva G., Soto H., Zlotnik A., Nakano H., Kakiuchi T., Hedrick J.A., Lira S.A.
J. Exp. Med. 190:1183-1188(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
Tissue: Embryonic stem cell.
[4]"Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation."
Nakano H., Gunn M.D.
J. Immunol. 166:361-369(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Ola.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Lymph node and Spleen.
[6]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and FVB/N.
Tissue: Colon and Mammary gland.
[8]"A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes."
Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D., Williams L.T.
Proc. Natl. Acad. Sci. U.S.A. 95:258-263(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001980 mRNA. Translation: AAB86595.1.
AF006637 mRNA. Translation: AAB61440.1.
AF171086 Genomic DNA. Translation: AAF16901.1.
AF035684 Genomic DNA. Translation: AAC82613.1.
AF307985 Genomic DNA. Translation: AAG45833.1.
AK144258 mRNA. Translation: BAE25802.1.
AK155924 mRNA. Translation: BAE33505.1.
AL772334 Genomic DNA. No translation available.
BC025974 mRNA. Translation: AAH25974.1.
BC028747 mRNA. Translation: AAH28747.1.
BC038120 mRNA. Translation: AAH38120.1.
CCDSCCDS18081.1.
RefSeqNP_001180596.1. NM_001193667.1.
NP_035254.1. NM_011124.4.
UniGeneMm.220853.
Mm.458815.

3D structure databases

ProteinModelPortalP84444.
SMRP84444. Positions 24-92.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000103647.

Proteomic databases

PaxDbP84444.
PRIDEP84444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000095114; ENSMUSP00000092732; ENSMUSG00000094686.
ENSMUST00000178919; ENSMUSP00000136780; ENSMUSG00000095320.
GeneID100504362.
18829.
KEGGmmu:100504362.
mmu:18829.
UCSCuc008sny.2. mouse.

Organism-specific databases

CTD18829.
MGIMGI:1349183. Ccl21a.

Phylogenomic databases

eggNOGNOG46090.
GeneTreeENSGT00730000111297.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidP84444.
KOK16062.
OMACCLKYSL.
OrthoDBEOG7GXPDG.
PhylomeDBP84444.
TreeFamTF338224.

Gene expression databases

BgeeP84444.
CleanExMM_CCL21B.
GenevestigatorP84444.

Family and domain databases

InterProIPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
ProtoNetSearch...

Other

NextBio295204.
PROP84444.
SOURCESearch...

Entry information

Entry nameCC21A_MOUSE
AccessionPrimary (citable) accession number: P84444
Secondary accession number(s): O09002 expand/collapse secondary AC list , O09006, Q3U1J2, Q91V84
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot