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P84309 (ADCY5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylate cyclase type 5

EC=4.6.1.1
Alternative name(s):
ATP pyrophosphate-lyase 5
Adenylate cyclase type V
Adenylyl cyclase 5
Gene names
Name:Adcy5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a membrane-bound, calcium-inhibitable adenylyl cyclase.

Catalytic activity

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactor

Binds 2 magnesium ions per subunit By similarity. UniProtKB P30803

Enzyme regulation

Inhibition by calcium in the submicromolar concentration range By similarity. Phosphorylation by RAF1 results in its activation By similarity. UniProtKB Q04400

Subunit structure

Part of a complex containing AKAP5, ADCY6, PDE4C and PKD2. Interacts with RAF1 By similarity. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein By similarity. Cell projectioncilium Ref.3.

Post-translational modification

Phosphorylated by RAF1 By similarity.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 2 guanylate cyclase domains.

Ontologies

Keywords
   Biological processcAMP biosynthesis
   Cellular componentCell projection
Cilium
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLyase
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenosine receptor signaling pathway

Inferred from genetic interaction PubMed 12223546. Source: MGI

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from genetic interaction PubMed 12223546. Source: MGI

adenylate cyclase-activating dopamine receptor signaling pathway

Inferred from genetic interaction PubMed 12223546. Source: MGI

adenylate cyclase-inhibiting dopamine receptor signaling pathway

Inferred from genetic interaction PubMed 12223546. Source: MGI

cAMP biosynthetic process

Traceable author statement PubMed 7766992. Source: MGI

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

locomotory behavior

Inferred from mutant phenotype PubMed 12665504. Source: MGI

neuromuscular process controlling balance

Inferred from mutant phenotype PubMed 12665504. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay PubMed 17593019. Source: MGI

plasma membrane

Inferred from direct assay PubMed 18164588. Source: BHF-UCL

primary cilium

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

adenylate cyclase activity

Inferred from direct assay PubMed 17593019. Source: MGI

adenylate cyclase binding

Inferred from physical interaction PubMed 18164588. Source: BHF-UCL

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein heterodimerization activity

Inferred from physical interaction PubMed 18164588. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P84309-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P84309-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1259-1262: PPLS → LGHDGVVGKL...GSEQKKIFIK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12621262Adenylate cyclase type 5
PRO_0000195695

Regions

Topological domain1 – 196196Cytoplasmic Potential
Transmembrane197 – 21721Helical; Potential
Transmembrane243 – 26321Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane300 – 32021Helical; Potential
Transmembrane326 – 34621Helical; Potential
Transmembrane375 – 39521Helical; Potential
Topological domain396 – 763368Cytoplasmic Potential
Transmembrane764 – 78421Helical; Potential
Transmembrane790 – 81021Helical; Potential
Transmembrane837 – 85721Helical; Potential
Topological domain858 – 91053Extracellular Potential
Transmembrane911 – 93121Helical; Potential
Transmembrane936 – 95621Helical; Potential
Transmembrane985 – 100521Helical; Potential
Topological domain1006 – 1262257Cytoplasmic Potential
Domain470 – 597128Guanylate cyclase 1
Domain1072 – 1211140Guanylate cyclase 2
Coiled coil1019 – 104527 Potential
Compositional bias64 – 674Poly-Gln
Compositional bias141 – 1477Poly-Ala

Sites

Metal binding4751Magnesium 1 By similarity
Metal binding4751Magnesium 2 By similarity
Metal binding4761Magnesium 2; via carbonyl oxygen By similarity

Amino acid modifications

Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation8341N-linked (GlcNAc...) Potential
Glycosylation8711N-linked (GlcNAc...) Potential
Glycosylation8881N-linked (GlcNAc...) Potential
Glycosylation9731N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1259 – 12624PPLS → LGHDGVVGKLKAGLGVSMEL KGLLFHCGEVTPPHNVWGTG TGRRVACAILSPHLHAQRQC PVRETGLLTREARGHQARSS GSEQKKIFIK in isoform 2.
VSP_022224

Experimental info

Sequence conflict251G → S in AAH90846. Ref.2
Sequence conflict6861G → D in BAE28048. Ref.1
Sequence conflict9241V → M in AAH90846. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 342966E7CA67E28D

FASTA1,262139,122
        10         20         30         40         50         60 
MSGSKSVSPP GYAAQTAASP APRGGPEHRA AWGEADSRAN GYPHAPGGST RGSTKRSGGA 

        70         80         90        100        110        120 
VTPQQQQRLA SRWRGGDDDE DPPLSGDDPL AGGFGFSFRS KSAWQERGGD DGGRGSRRQR 

       130        140        150        160        170        180 
RGAAGGGSTR APPAGGSGSS AAAAAAAGGT EVRPRSVELG LEERRGKGRA AEELEPGTGI 

       190        200        210        220        230        240 
VEDGDGSEDG GSSVASGSGT GAVLSLGACC LALLQIFRSK KFPSDKLERL YQRYFFRLNQ 

       250        260        270        280        290        300 
SSLTMLMAVL VLVCLVMLAF HAARPPLQIA YLAVLAAAVG VILIMAVLCN RAAFHQDHMG 

       310        320        330        340        350        360 
LACYALIAVV LAVQVVGLLL PQPRSASEGI WWTVFFIYTI YTLLPVRMRA AVLSGVLLSA 

       370        380        390        400        410        420 
LHLAISLHTN SQDQFLLKQL VSNVLIFSCT NIVGVCTHYP AEVSQRQAFQ ETRECIQARL 

       430        440        450        460        470        480 
HSQRENQQQE RLLLSVLPRH VAMEMKADIN AKQEDMMFHK IYIQKHDNVS ILFADIEGFT 

       490        500        510        520        530        540 
SLASQCTAQE LVMTLNELFA RFDKLAAENH CLRIKILGDC YYCVSGLPEA RADHAHCCVE 

       550        560        570        580        590        600 
MGMDMIEAIS LVREVTGVNV NMRVGIHSGR VHCGVLGLRK WQFDVWSNDV TLANHMEAGG 

       610        620        630        640        650        660 
KAGRIHITKA TLNYLNGDYE VEPGCGGDRN AYLKEHSIET FLILSCTQKR KEEKAMIAKM 

       670        680        690        700        710        720 
NRQRTNSIGH NPPHWGAERP FYNHLGGNQV SKEMKRMGFE DPKDKNAQES ANPEDEVDEF 

       730        740        750        760        770        780 
LGRAIDARSI DRLRSEHVRK FLLTFREPDL EKKYSKQVDD RFGAYVACAS LVFLFICFVQ 

       790        800        810        820        830        840 
ITIVPHSLFM LSFYLSCFLL LALVVFVSVI YACVKLFPTP LQTLSRKIVR SKKNSTLVGV 

       850        860        870        880        890        900 
FTITLVFLSA FVNMFMCNSK NLVGCLAEEH NITVNQVNAC HVMESAFNYS LGDEQGFCGS 

       910        920        930        940        950        960 
PQPNCNFPEY FTYSVLLSLL ACSVFLQISC IGKLVLMLAI EFIYVLIVEV PGVTLFDNAD 

       970        980        990       1000       1010       1020 
LLVTANAIDF SNNGTSQCPE HATKVALKVV TPIIISVFVL ALYLHAQQVE STARLDFLWK 

      1030       1040       1050       1060       1070       1080 
LQATEEKEEM EELQAYNRRL LHNILPKDVA AHFLARERRN DELYYQSCEC VAVMFASIAN 

      1090       1100       1110       1120       1130       1140 
FSEFYVELEA NNEGVECLRL LNEIIADFDE IISEDRFRQL EKIKTIGSTY MAASGLNDST 

      1150       1160       1170       1180       1190       1200 
YDKAGKTHIK AIADFAMKLM DQMKYINEHS FNNFQMKIGL NIGPVVAGVI GARKPQYDIW 

      1210       1220       1230       1240       1250       1260 
GNTVNVASRM DSTGVPDRIQ VTTDMYQVLA ANTYQLECRG VVKVKGKGEM MTYFLNGGPP 


LS 

« Hide

Isoform 2 [UniParc].

Checksum: B4997E3A2BC14305
Show »

FASTA1,348148,383

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Head.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain and Retina.
[3]"Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases."
Choi Y.H., Suzuki A., Hajarnis S., Ma Z., Chapin H.C., Caplan M.J., Pontoglio M., Somlo S., Igarashi P.
Proc. Natl. Acad. Sci. U.S.A. 108:10679-10684(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AKAP5; ADCY6; PDE4C AND PKD2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK147649 mRNA. Translation: BAE28048.1.
AK160942 mRNA. Translation: BAE36104.1.
BC035550 mRNA. No translation available.
BC090846 mRNA. Translation: AAH90846.1.
RefSeqNP_001012783.3. NM_001012765.4.
UniGeneMm.41137.

3D structure databases

ProteinModelPortalP84309.
SMRP84309. Positions 455-644, 1065-1256.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP84309.

PTM databases

PhosphoSiteP84309.

Proteomic databases

PaxDbP84309.
PRIDEP84309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000114913; ENSMUSP00000110563; ENSMUSG00000022840. [P84309-1]
GeneID224129.
KEGGmmu:224129.
UCSCuc007zbj.1. mouse. [P84309-1]

Organism-specific databases

CTD111.
MGIMGI:99673. Adcy5.

Phylogenomic databases

eggNOGCOG2114.
GeneTreeENSGT00680000099709.
HOGENOMHOG000006941.
HOVERGENHBG050458.
KOK08045.
OMAVRSKMNS.
OrthoDBEOG7B8S30.
PhylomeDBP84309.
TreeFamTF313845.

Gene expression databases

BgeeP84309.
CleanExMM_ADCY5.
GenevestigatorP84309.

Family and domain databases

Gene3D3.30.70.1230. 2 hits.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR009398. Adenylate_cyclase-like.
[Graphical view]
PfamPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
SMARTSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMSSF55073. SSF55073. 2 hits.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio377103.
PROP84309.
SOURCESearch...

Entry information

Entry nameADCY5_MOUSE
AccessionPrimary (citable) accession number: P84309
Secondary accession number(s): Q3TU67, Q3UH09, Q5BL06
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 9, 2007
Last modified: April 16, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot