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Protein

Histone H3.3

Gene

His3.3A

more
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes and is specifically enriched in modifications associated with active chromatin. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.1 Publication

Miscellaneous

This histone is the predominant form in non-dividing cells.

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • nucleosomal DNA binding Source: GO_Central
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

  • male meiotic nuclear division Source: FlyBase
  • nucleosome assembly Source: GO_Central

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H3.3
Alternative name(s):
H3.3Q
H3.A/B
Gene namesi
Name:His3.3A
ORF Names:CG5825
AND
Name:His3.3B
ORF Names:CG8989
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componentsi: Chromosome 2L, Chromosome X

Organism-specific databases

FlyBaseiFBgn0014857. His3.3A.
FBgn0004828. His3.3B.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002213022 – 136Histone H3.3Add BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5N6,N6,N6-trimethyllysine; alternate1 Publication1
Modified residuei5N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei5N6-methyllysine; alternate1 Publication1
Modified residuei10N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei10N6-acetyllysine; alternate2 Publications1
Modified residuei10N6-methyllysine; alternate1 Publication1
Modified residuei11PhosphoserineBy similarity1
Modified residuei15N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei15N6-acetyllysine; alternate3 Publications1
Modified residuei15N6-methyllysine; alternate1 Publication1
Modified residuei19N6-acetyllysine1 Publication1
Modified residuei24N6-acetyllysine1 Publication1
Modified residuei28N6,N6,N6-trimethyllysine; alternate1 Publication1
Modified residuei28N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei28N6-methyllysine; alternate1 Publication1
Modified residuei37N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei37N6-methyllysine; alternate1 Publication1
Modified residuei38N6,N6-dimethyllysine; alternate1 Publication1
Modified residuei38N6-methyllysine; alternate1 Publication1
Modified residuei80N6,N6-dimethyllysine; alternate2 Publications1
Modified residuei80N6-methyllysine; alternate2 Publications1

Post-translational modificationi

Phosphorylation at Ser-11 by ial/aurora-B during mitosis and meiosis is crucial for chromosome condensation and cell-cycle progression. Phosphorylation at Ser-11 by JIL-1 during interphase is linked to gene activation and restricts the formation of heterochromatin at inappropriate sites. Phosphorylation at Ser-11 is enriched on male X chromosome compared to the autosome.4 Publications
Acetylation is generally linked to gene activation. Acetylated on Lys-15 during prophase I of meiosis. Phosphorylation of H2A 'Thr-119' is a prerequisite for H3 Lys-15 acetylation. Acetylation on Lys-15 is enriched on male X chromosome compared to the autosome.3 Publications
Methylation at Lys-5 or Lys-80 is generally associated with active chromatin. Methylation at Lys-80 by gpp occurs at low levels in specific developmental stages and tissues undergoing active cell division, and at highest levels in epidermal cells undergoing differentiation.2 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP84249.
PRIDEiP84249.

Expressioni

Gene expression databases

BgeeiFBgn0004828.
ExpressionAtlasiP84249. differential.
GenevisibleiP84249. DM.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi58303. 10 interactors.
59912. 19 interactors.
IntActiP84249. 8 interactors.
MINTiMINT-840146.
STRINGi7227.FBpp0305663.

Structurei

3D structure databases

ProteinModelPortaliP84249.
SMRiP84249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H3 family.Curated

Phylogenomic databases

eggNOGiKOG1745. Eukaryota.
COG2036. LUCA.
GeneTreeiENSGT00760000118967.
InParanoidiP84249.
KOiK11253.
OMAiKRIEPEY.
OrthoDBiEOG091G0XGD.
PhylomeDBiP84249.

Family and domain databases

InterProiView protein in InterPro
IPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000164. Histone_H3/CENP-A.
PANTHERiPTHR11426. PTHR11426. 1 hit.
PfamiView protein in Pfam
PF00125. Histone. 1 hit.
PRINTSiPR00622. HISTONEH3.
SMARTiView protein in SMART
SM00428. H3. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00322. HISTONE_H3_1. 1 hit.
PS00959. HISTONE_H3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTKQTARK STGGKAPRKQ LATKAARKSA PSTGGVKKPH RYRPGTVALR
60 70 80 90 100
EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSAAI GALQEASEAY
110 120 130
LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
Length:136
Mass (Da):15,328
Last modified:January 23, 2007 - v2
Checksum:i5158ED279E6F9E1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53822 mRNA. Translation: CAA37819.1.
X82257 mRNA. Translation: CAA57712.1.
X81205 Genomic DNA. Translation: CAA57077.1.
X81207 Genomic DNA. Translation: CAA57080.1.
AE014298 Genomic DNA. Translation: AAF46452.1.
AE014134 Genomic DNA. Translation: AAF52213.1.
AE014298 Genomic DNA. Translation: AAN09245.1.
AE014298 Genomic DNA. Translation: AAO41645.1.
AY071057 mRNA. Translation: AAL48679.1.
AY118325 mRNA. Translation: AAM48354.1.
AY119629 mRNA. Translation: AAM50283.1.
PIRiS61220.
RefSeqiNP_001259374.1. NM_001272445.2.
NP_001260082.1. NM_001273153.1.
NP_511095.1. NM_078540.5.
NP_523479.1. NM_078755.3.
NP_723056.2. NM_164624.3.
NP_727314.1. NM_167180.4.
NP_788892.1. NM_176719.1.
UniGeneiDm.2931.
Dm.35099.
Dm.7418.

Genome annotation databases

EnsemblMetazoaiFBtr0071345; FBpp0071280; FBgn0004828.
FBtr0071346; FBpp0071281; FBgn0004828.
FBtr0071347; FBpp0071282; FBgn0004828.
FBtr0079010; FBpp0078649; FBgn0014857.
FBtr0333536; FBpp0305716; FBgn0014857.
FBtr0333537; FBpp0305717; FBgn0014857.
FBtr0340343; FBpp0309302; FBgn0004828.
GeneIDi31848.
33736.
KEGGidme:Dmel_CG5825.
dme:Dmel_CG8989.

Similar proteinsi

Entry informationi

Entry nameiH33_DROME
AccessioniPrimary (citable) accession number: P84249
Secondary accession number(s): A4V466
, P06351, P33155, Q9V3W4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families