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Protein

Profilin

Gene
N/A
Organism
Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).By similarity

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin1 Publication
Alternative name(s):
Allergen: Cit s 21 Publication
OrganismiCitrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)
Taxonomic identifieri2711 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. Induces histamine release from basophils of profilin-allergic patients.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1173. Cit s 2.
3200. Cit s 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 131130Profilin1 PublicationPRO_0000199626Add
BLAST

Interactioni

Subunit structurei

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.By similarity

Structurei

3D structure databases

ProteinModelPortaliP84177.
SMRiP84177. Positions 2-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Family and domain databases

InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWQAYVDDH LMCDIDGNRL TAAAILGQDG SVWSQSATFP AFRLEEIAAI
60 70 80 90 100
LKDFDQPGTL APTGLFLGGT KYMVIQGEAG AVIRGKKGSG GIIVKKTNQA
110 120 130
LIIGIYDEPL TPGQCNMIVE RLGDYLIEQG L
Length:131
Mass (Da):14,060
Last modified:October 13, 2009 - v2
Checksum:i163FC690C9444866
GO

Mass spectrometryi

Molecular mass is 14137 Da from positions 2 - 131. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti60 – 601L → F.1 Publication
Natural varianti93 – 931I → T.1 Publication
Natural varianti114 – 1141Q → R.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ865015 mRNA. Translation: CAI23765.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ865015 mRNA. Translation: CAI23765.1.

3D structure databases

ProteinModelPortaliP84177.
SMRiP84177. Positions 2-131.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei1173. Cit s 2.
3200. Cit s 2.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen."
    Lopez-Torrejon G., Ibanez M.D., Ahrazem O., Sanchez-Monge R., Sastre J., Lombardero M., Barber D., Salcedo G.
    Allergy 60:1424-1429(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, MASS SPECTROMETRY, ALLERGEN, VARIANTS PHE-60; THR-93 AND ARG-114.
    Strain: cv. Navel late1 Publication.
    Tissue: Fruit flesh1 Publication and Peelings1 Publication.
  2. "Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits."
    Crespo J.F., Retzek M., Foetisch K., Sierra-Maestro E., Cid-Sanchez A.B., Pascual C.Y., Conti A., Feliu A., Rodriguez J., Vieths S., Scheurer S.
    Mol. Nutr. Food Res. 50:282-290(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALLERGEN.

Entry informationi

Entry nameiPROF1_CITSI
AccessioniPrimary (citable) accession number: P84177
Secondary accession number(s): Q5TIM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 13, 2009
Last modified: March 4, 2015
This is version 34 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.