P84177 (PROF1_CITSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Profilin Alternative name(s): Allergen=Cit s 2
Organism	Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)
Taxonomic identifier	2711 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Sapindales › Rutaceae › Citrus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG By similarity. UniProtKB P49233
Subunit structure	Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio By similarity. UniProtKB P49233
Subcellular location	Cytoplasm › cytoskeleton By similarity UniProtKB P49233.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Induces histamine release from basophils of profilin-allergic patients. Ref.1 Ref.2
Sequence similarities	Belongs to the profilin family.
Mass spectrometry	Molecular mass is 14137 Da from positions 2 - 131. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Disease	Allergen
Ligand	Actin-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 131	130	Profilin Ref.1		PRO_0000199626
Natural variations
	Natural variant	60	1	L → F. Ref.1
	Natural variant	93	1	I → T. Ref.1
	Natural variant	114	1	Q → R. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P84177 [UniParc]. Last modified October 13, 2009. Version 2. Checksum: 163FC690C9444866	FASTA	131	14,060
	10 20 30 40 50 60 MSWQAYVDDH LMCDIDGNRL TAAAILGQDG SVWSQSATFP AFRLEEIAAI LKDFDQPGTL 70 80 90 100 110 120 APTGLFLGGT KYMVIQGEAG AVIRGKKGSG GIIVKKTNQA LIIGIYDEPL TPGQCNMIVE 130 RLGDYLIEQG L

References

[1]

"Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen." Lopez-Torrejon G., Ibanez M.D., Ahrazem O., Sanchez-Monge R., Sastre J., Lombardero M., Barber D., Salcedo G. Allergy 60:1424-1429(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, MASS SPECTROMETRY, ALLERGEN, VARIANTS PHE-60; THR-93 AND ARG-114. Strain: cv. Navel late. Tissue: Fruit flesh and Peelings.

[2]

"Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits." Crespo J.F., Retzek M., Foetisch K., Sierra-Maestro E., Cid-Sanchez A.B., Pascual C.Y., Conti A., Feliu A., Rodriguez J., Vieths S., Scheurer S. Mol. Nutr. Food Res. 50:282-290(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ALLERGEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ865015 mRNA. Translation: CAI23765.1.
3D structure databases
ProteinModelPortal	P84177.
SMR	P84177. Positions 2-131.
ModBase	Search...
Protein family/group databases
Allergome	1173. Cit s 2. 3200. Cit s 2.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR027310. Profilin_CS. IPR005455. Profilin_eukaryotes/bac. [Graphical view]
PANTHER	PTHR11604. PTHR11604. 1 hit.
Pfam	PF00235. Profilin. 1 hit. [Graphical view]
PRINTS	PR00392. PROFILIN. PR01640. PROFILINPLNT.
SMART	SM00392. PROF. 1 hit. [Graphical view]
SUPFAM	SSF55770. Profilin. 1 hit.
PROSITE	PS00414. PROFILIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROF1_CITSI

Accession

Primary (citable) accession number: P84177
Secondary accession number(s): Q5TIM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 13, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program