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P84177 (PROF1_CITSI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Profilin
Alternative name(s):
Allergen=Cit s 2
OrganismCitrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)
Taxonomic identifier2711 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG By similarity. UniProtKB P49233

Subunit structure

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio By similarity. UniProtKB P49233

Subcellular location

Cytoplasmcytoskeleton By similarity UniProtKB P49233.

Allergenic properties

Causes an allergic reaction in human. Binds to IgE. Induces histamine release from basophils of profilin-allergic patients. Ref.1 Ref.2

Sequence similarities

Belongs to the profilin family.

Mass spectrometry

Molecular mass is 14137 Da from positions 2 - 131. Determined by MALDI. Ref.1

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   LigandActin-binding
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 131130Profilin Ref.1
PRO_0000199626

Natural variations

Natural variant601L → F. Ref.1
Natural variant931I → T. Ref.1
Natural variant1141Q → R. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P84177 [UniParc].

Last modified October 13, 2009. Version 2.
Checksum: 163FC690C9444866

FASTA13114,060
        10         20         30         40         50         60 
MSWQAYVDDH LMCDIDGNRL TAAAILGQDG SVWSQSATFP AFRLEEIAAI LKDFDQPGTL 

        70         80         90        100        110        120 
APTGLFLGGT KYMVIQGEAG AVIRGKKGSG GIIVKKTNQA LIIGIYDEPL TPGQCNMIVE 

       130 
RLGDYLIEQG L 

« Hide

References

[1]"Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen."
Lopez-Torrejon G., Ibanez M.D., Ahrazem O., Sanchez-Monge R., Sastre J., Lombardero M., Barber D., Salcedo G.
Allergy 60:1424-1429(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, MASS SPECTROMETRY, ALLERGEN, VARIANTS PHE-60; THR-93 AND ARG-114.
Strain: cv. Navel late.
Tissue: Fruit flesh and Peelings.
[2]"Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits."
Crespo J.F., Retzek M., Foetisch K., Sierra-Maestro E., Cid-Sanchez A.B., Pascual C.Y., Conti A., Feliu A., Rodriguez J., Vieths S., Scheurer S.
Mol. Nutr. Food Res. 50:282-290(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ALLERGEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ865015 mRNA. Translation: CAI23765.1.

3D structure databases

ProteinModelPortalP84177.
SMRP84177. Positions 2-131.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome1173. Cit s 2.
3200. Cit s 2.0101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR027310. Profilin_CS.
IPR005455. Profilin_eukaryotes/bac.
[Graphical view]
PANTHERPTHR11604. PTHR11604. 1 hit.
PfamPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMSSF55770. SSF55770. 1 hit.
PROSITEPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROF1_CITSI
AccessionPrimary (citable) accession number: P84177
Secondary accession number(s): Q5TIM0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 13, 2009
Last modified: February 19, 2014
This is version 30 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Allergens

Nomenclature of allergens and list of entries