ID   MEX67_CANAL             Reviewed;         617 AA.
AC   P84149; A0A1D8PSN9; Q5A6R6;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 2.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=mRNA export factor MEX67;
GN   Name=MEX67 {ECO:0000250|UniProtKB:Q99257};
GN   OrderedLocusNames=CAALFM_CR04050CA; ORFNames=CaO19.488, CaO19.8118;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 295-512 IN COMPLEX WITH MTR2.
RX   PubMed=14504280; DOI=10.1074/jbc.m308275200;
RA   Senay C., Ferrari P., Rocher C., Rieger K.J., Winter J., Platel D.,
RA   Bourne Y.;
RT   "The Mtr2-Mex67 NTF2-like domain complex. Structural insights into a dual
RT   role of Mtr2 for yeast nuclear export.";
RL   J. Biol. Chem. 278:48395-48403(2003).
CC   -!- FUNCTION: Involved in the export of mRNA from the nucleus to the
CC       cytoplasm. {ECO:0000250|UniProtKB:Q99257, ECO:0000255|PROSITE-
CC       ProRule:PRU00611}.
CC   -!- SUBUNIT: Interacts with nucleoporin complex protein MTR2.
CC       {ECO:0000269|PubMed:14504280}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00611}.
CC       Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00611}. Note=Localizes at
CC       both the nuclear and cytoplasmic site of the pores. Shuttles between
CC       the nucleus and the cytoplasm. {ECO:0000255|PROSITE-ProRule:PRU00611}.
CC   -!- DOMAIN: The NTF2 domain heterodimerizes with MTR2. The formation of
CC       this heterodimer is essential for mRNA export and binds to all of the
CC       nucleoporin-FG-repeats. {ECO:0000250|UniProtKB:Q99257,
CC       ECO:0000269|PubMed:14504280}.
CC   -!- DOMAIN: The RNA-binding domain is conserved in most NXF proteins but
CC       may be absent in yeasts.
CC   -!- SIMILARITY: Belongs to the NXF family. {ECO:0000255}.
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DR   EMBL; CP017630; AOW31143.1; -; Genomic_DNA.
DR   RefSeq; XP_717351.1; XM_712258.2.
DR   PDB; 1Q40; X-ray; 1.95 A; B/D=295-512.
DR   PDBsum; 1Q40; -.
DR   AlphaFoldDB; P84149; -.
DR   SMR; P84149; -.
DR   BioGRID; 1224015; 1.
DR   STRING; 237561.P84149; -.
DR   EnsemblFungi; CR_04050C_A-T; CR_04050C_A-T-p1; CR_04050C_A.
DR   GeneID; 3641020; -.
DR   KEGG; cal:CAALFM_CR04050CA; -.
DR   CGD; CAL0000181331; MEX67.
DR   VEuPathDB; FungiDB:CR_04050C_A; -.
DR   eggNOG; KOG3763; Eukaryota.
DR   HOGENOM; CLU_024991_1_1_1; -.
DR   InParanoid; P84149; -.
DR   OMA; YGGHEAW; -.
DR   OrthoDB; 10284at2759; -.
DR   EvolutionaryTrace; P84149; -.
DR   PRO; PR:P84149; -.
DR   Proteomes; UP000000559; Chromosome R.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005643; C:nuclear pore; IEA:EnsemblFungi.
DR   GO; GO:0042272; C:nuclear RNA export factor complex; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0015288; F:porin activity; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0030619; F:U1 snRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0030620; F:U2 snRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0030621; F:U4 snRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0030623; F:U5 snRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0017070; F:U6 snRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR   GO; GO:0000055; P:ribosomal large subunit export from nucleus; IEA:EnsemblFungi.
DR   GO; GO:0000056; P:ribosomal small subunit export from nucleus; IEA:EnsemblFungi.
DR   CDD; cd14342; UBA_TAP-C; 1.
DR   Gene3D; 3.10.450.50; -; 1.
DR   Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR   Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 1.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR040736; Mex67_RRM.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR   InterPro; IPR030217; NXF_fam.
DR   InterPro; IPR005637; TAP_C_dom.
DR   InterPro; IPR009060; UBA-like_sf.
DR   PANTHER; PTHR10662; NUCLEAR RNA EXPORT FACTOR; 1.
DR   PANTHER; PTHR10662:SF22; NUCLEAR RNA EXPORT FACTOR 1; 1.
DR   Pfam; PF18444; RRM_9; 1.
DR   Pfam; PF03943; TAP_C; 1.
DR   SMART; SM00804; TAP_C; 1.
DR   SUPFAM; SSF52058; L domain-like; 1.
DR   SUPFAM; SSF54427; NTF2-like; 1.
DR   SUPFAM; SSF46934; UBA-like; 1.
DR   PROSITE; PS51450; LRR; 3.
DR   PROSITE; PS50177; NTF2_DOMAIN; 1.
DR   PROSITE; PS51281; TAP_C; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Leucine-rich repeat; mRNA transport; Nucleus;
KW   Reference proteome; Repeat; Transport.
FT   CHAIN           1..617
FT                   /note="mRNA export factor MEX67"
FT                   /id="PRO_0000220540"
FT   REPEAT          185..206
FT                   /note="LRR 1"
FT   REPEAT          211..232
FT                   /note="LRR 2"
FT   REPEAT          237..258
FT                   /note="LRR 3"
FT   DOMAIN          309..499
FT                   /note="NTF2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT   DOMAIN          565..617
FT                   /note="TAP-C"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00611"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          442..469
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          513..554
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        513..533
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           307..322
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   HELIX           325..331
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          337..342
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   TURN            364..369
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   TURN            373..375
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   HELIX           379..384
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   HELIX           389..398
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          402..404
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   TURN            407..409
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   HELIX           411..413
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          415..420
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          427..439
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          460..464
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          474..483
FT                   /evidence="ECO:0007829|PDB:1Q40"
FT   STRAND          491..501
FT                   /evidence="ECO:0007829|PDB:1Q40"
SQ   SEQUENCE   617 AA;  68257 MW;  F014C89D5F56AC2D CRC64;
     MSYRGRGGGY NNNRGQFSSG PHQHQQNVDS FVAANQYPIE IMGWNGASSG ECINFISRKC
     KVIVSNYSVD SNSGVLKGYV KNESQANTLL NWSGVKFAGQ SLRFSKGVSN ISNQMGGGAS
     TGSQSTIETI SQFLKARYQP EIKMLNLSNV KQDPTLTAQG FFGSLSVSSK FFPALMKVAS
     DLKLDVDSID LSNNELQDLQ TLTSMAQTFP KLQNLSLQNN NFTKIKVFET WRHKLNFLRE
     LILFNNPIVQ TNDPAEIQTI KLELMKSFPR LVVLSGEILR NEQVLIANLS FPFESPETMF
     FQDEDSRNLA TNFIANYLKL WDANRSELMI LYQNESQFSM QVDSSHPHLI ESGNSGYSGS
     TDFGYYLNNS RNLTRVSSIK ARMAKLSIGQ EQIYKSFQQL PKTRHDIIAT PELFSMEVYK
     FPTLNGIMIT LHGSFDEVAQ PEVDGSASSA PSGPRGGSRY HSGPKHKRIP LSKKSFDRTF
     VVIPGPNGSM IVASDTLLIR PYTSDFPWKV QKLPSNPTAA TPGVSATSTP SPLPPTTITT
     PQLAPPGTGP TTADLPADIK ARLNQIQQEL LVKILLETKL NINYGIMLCE QSNWDYQQAS
     VNFKNSAASL PSDAFVQ
//