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P84147

- P84147_STRSM

UniProt

P84147 - P84147_STRSM

Protein
Submitted name:

Phospholipase D

Gene
N/A
Organism
Streptomyces sp. (strain PMF)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671NucleophileImported
    Binding sitei184 – 1841Phosphate 1
    Sitei199 – 1991Important for catalytic activityImported
    Binding sitei300 – 3001Phosphate 2
    Active sitei440 – 4401Proton donor/acceptorImported
    Binding sitei457 – 4571Phosphate 1
    Sitei465 – 4651Important for catalytic activityImported

    GO - Molecular functioni

    1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
    2. phospholipase D activity Source: UniProtKB-EC

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Phospholipase DImported (EC:3.1.4.4Imported)
    OrganismiStreptomyces sp. (strain PMF)Imported
    Taxonomic identifieri172564 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1F0IX-ray1.40A4-504[»]
    1V0RX-ray1.70A1-506[»]
    1V0SX-ray1.75A1-506[»]
    1V0TX-ray1.53A1-506[»]
    1V0UX-ray1.42A1-506[»]
    1V0VX-ray1.70A1-506[»]
    1V0WX-ray1.35A1-506[»]
    1V0YX-ray1.71A1-506[»]
    ProteinModelPortaliP84147.
    SMRiP84147. Positions 3-506.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP84147.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni167 – 1693Phosphate 1 binding
    Regioni440 – 4423Phosphate 1 binding

    Family and domain databases

    InterProiIPR025202. PLD-like_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    [Graphical view]
    PfamiPF13091. PLDc_2. 1 hit.
    [Graphical view]
    SMARTiSM00155. PLDc. 2 hits.
    [Graphical view]
    PROSITEiPS50035. PLD. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P84147-1 [UniParc]FASTAAdd to Basket

    « Hide

    ADSATPHLDA VEQTLRQVSP GLEGDVWERT SGNKLDGSAA DPSDWLLQTP    50
    GCWGDDKCAD RVGTKRLLAK MTENIGNATR TVDISTLAPF PNGAFQDAIV 100
    AGLKESAAKG NKLKVRILVG AAPVYHMNVI PSKYRDELTA KLGKAAENIT 150
    LNVASMTTSK TAFSWNHSKI LVVDGQSALT GGINSWKDDY LDTTHPVSDV 200
    DLALTGPAAG SAGRYLDTLW TWTCQNKSNI ASVWFAASGN AGCMPTMHKD 250
    TNPKASPATG NVPVIAVGGL GVGIKDVDPK STFRPDLPTA SDTKCVVGLH 300
    DNTNADRDYD TVNPEESALR ALVASAKGHI EISQQDLNAT CPPLPRYDIR 350
    LYDALAAKMA AGVKVRIVVS DPANRGAVGS GGYSQIKSLS EISDTLRNRL 400
    ANITGGQQAA KTAMCSNLQL ATFRSSPNGK WADGHPYAQH HKLVSVDSST 450
    FYIGSKNLYP SWLQDFGYIV ESPEAAKQLD AKLLDPQWKY SQETATVDYA 500
    RGICNA 506
    Length:506
    Mass (Da):54,005
    Last modified:September 13, 2004 - v1
    Checksum:iC7420055940CBF27
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1F0I X-ray 1.40 A 4-504 [» ]
    1V0R X-ray 1.70 A 1-506 [» ]
    1V0S X-ray 1.75 A 1-506 [» ]
    1V0T X-ray 1.53 A 1-506 [» ]
    1V0U X-ray 1.42 A 1-506 [» ]
    1V0V X-ray 1.70 A 1-506 [» ]
    1V0W X-ray 1.35 A 1-506 [» ]
    1V0Y X-ray 1.71 A 1-506 [» ]
    ProteinModelPortali P84147.
    SMRi P84147. Positions 3-506.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P84147.

    Family and domain databases

    InterProi IPR025202. PLD-like_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    [Graphical view ]
    Pfami PF13091. PLDc_2. 1 hit.
    [Graphical view ]
    SMARTi SM00155. PLDc. 2 hits.
    [Graphical view ]
    PROSITEi PS50035. PLD. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The first crystal structure of a phospholipase D."
      Leiros I., Secundo F., Zambonelli C., Servi S., Hough E.
      Structure 8:655-667(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 4-504.
    2. "The reaction mechanism of phospholipase D from Streptomyces sp. strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product."
      Leiros I., McSweeney S., Hough E.
      J. Mol. Biol. 339:805-820(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS), ACTIVE SITE.

    Entry informationi

    Entry nameiP84147_STRSM
    AccessioniPrimary (citable) accession number: P84147
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3