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P84147

- P84147_STRSM

UniProt

P84147 - P84147_STRSM

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Protein
Submitted name:

Phospholipase D

Gene
N/A
Organism
Streptomyces sp. (strain PMF)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671NucleophileImported
Binding sitei184 – 1841Phosphate 1
Sitei199 – 1991Important for catalytic activityImported
Binding sitei300 – 3001Phosphate 2
Active sitei440 – 4401Proton donor/acceptorImported
Binding sitei457 – 4571Phosphate 1
Sitei465 – 4651Important for catalytic activityImported

GO - Molecular functioni

  1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  2. phospholipase D activity Source: UniProtKB-EC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Submitted name:
Phospholipase DImported (EC:3.1.4.4Imported)
OrganismiStreptomyces sp. (strain PMF)Imported
Taxonomic identifieri172564 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F0IX-ray1.40A4-504[»]
1V0RX-ray1.70A1-506[»]
1V0SX-ray1.75A1-506[»]
1V0TX-ray1.53A1-506[»]
1V0UX-ray1.42A1-506[»]
1V0VX-ray1.70A1-506[»]
1V0WX-ray1.35A1-506[»]
1V0YX-ray1.71A1-506[»]
ProteinModelPortaliP84147.
SMRiP84147. Positions 3-506.

Miscellaneous databases

EvolutionaryTraceiP84147.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 1693Phosphate 1 binding
Regioni440 – 4423Phosphate 1 binding

Family and domain databases

InterProiIPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PfamiPF13091. PLDc_2. 1 hit.
[Graphical view]
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P84147-1 [UniParc]FASTAAdd to Basket

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ADSATPHLDA VEQTLRQVSP GLEGDVWERT SGNKLDGSAA DPSDWLLQTP    50
GCWGDDKCAD RVGTKRLLAK MTENIGNATR TVDISTLAPF PNGAFQDAIV 100
AGLKESAAKG NKLKVRILVG AAPVYHMNVI PSKYRDELTA KLGKAAENIT 150
LNVASMTTSK TAFSWNHSKI LVVDGQSALT GGINSWKDDY LDTTHPVSDV 200
DLALTGPAAG SAGRYLDTLW TWTCQNKSNI ASVWFAASGN AGCMPTMHKD 250
TNPKASPATG NVPVIAVGGL GVGIKDVDPK STFRPDLPTA SDTKCVVGLH 300
DNTNADRDYD TVNPEESALR ALVASAKGHI EISQQDLNAT CPPLPRYDIR 350
LYDALAAKMA AGVKVRIVVS DPANRGAVGS GGYSQIKSLS EISDTLRNRL 400
ANITGGQQAA KTAMCSNLQL ATFRSSPNGK WADGHPYAQH HKLVSVDSST 450
FYIGSKNLYP SWLQDFGYIV ESPEAAKQLD AKLLDPQWKY SQETATVDYA 500
RGICNA 506
Length:506
Mass (Da):54,005
Last modified:September 13, 2004 - v1
Checksum:iC7420055940CBF27
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F0I X-ray 1.40 A 4-504 [» ]
1V0R X-ray 1.70 A 1-506 [» ]
1V0S X-ray 1.75 A 1-506 [» ]
1V0T X-ray 1.53 A 1-506 [» ]
1V0U X-ray 1.42 A 1-506 [» ]
1V0V X-ray 1.70 A 1-506 [» ]
1V0W X-ray 1.35 A 1-506 [» ]
1V0Y X-ray 1.71 A 1-506 [» ]
ProteinModelPortali P84147.
SMRi P84147. Positions 3-506.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P84147.

Family and domain databases

InterProi IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view ]
Pfami PF13091. PLDc_2. 1 hit.
[Graphical view ]
SMARTi SM00155. PLDc. 2 hits.
[Graphical view ]
PROSITEi PS50035. PLD. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The first crystal structure of a phospholipase D."
    Leiros I., Secundo F., Zambonelli C., Servi S., Hough E.
    Structure 8:655-667(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 4-504 IN COMPLEX WITH PHOSPHATE.
  2. "The reaction mechanism of phospholipase D from Streptomyces sp. strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product."
    Leiros I., McSweeney S., Hough E.
    J. Mol. Biol. 339:805-820(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS), ACTIVE SITE.

Entry informationi

Entry nameiP84147_STRSM
AccessioniPrimary (citable) accession number: P84147
Entry historyi
Integrated into UniProtKB/TrEMBL: September 13, 2004
Last sequence update: September 13, 2004
Last modified: June 11, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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