P84141 (P84141_ARTAU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Chondroitinase (Chondroitin lyase) PDB 1RW9 PDB 1RWA PDB 1RWC PDB 1RWF PDB 1RWG PDB 1RWH
Organism	Arthrobacter aurescens PDB 1RW9 PDB 1RWA PDB 1RWC PDB 1RWF PDB 1RWG PDB 1RWH
Taxonomic identifier	43663 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 1RW9 PDB 1RWA PDB 1RWC PDB 1RWF PDB 1RWG PDB 1RWH
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro carbon-oxygen lyase activity, acting on polysaccharides Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	124 – 126	3	N-acetyl-D-glucosamine binding PDB 1RWC
Sites
	Binding site	242	1	N-acetyl-D-glucosamine PDB 1RWC
	Binding site	296	1	N-acetyl-D-glucosamine PDB 1RWC
	Binding site	300	1	N-acetyl-D-glucosamine PDB 1RWC

Sequences

	Sequence		Length	Mass (Da)
	P84141 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A461E54AF0F3D88A	FASTA	757	79,928
	10 20 30 40 50 60 EAEPGAAEFA ALRNRWVDQI TGRNVIQAGD PDFAKAITAL NNKAADSLAK LDVKKGRTSV 70 80 90 100 110 120 FTDLSLAKDA EMVTTYTRLS QLATAWATPT AAVFGDAAVL AAIKAGLADA NTLCYNDRKE 130 140 150 160 170 180 EVGNWWSWEI GVPRALADAM VLLHAELSAA ERTAYCAAID HFVPDPWLQF PPKRGKITSV 190 200 210 220 230 240 GANRVDLCQG IIIRSLAGED PTKLNHAVAG LSQVWQYVTS GDGIFRDGSF IQHSTTPYTG 250 260 270 280 290 300 SYGVVLLTGL SKLFSLLGGT AFEVSDPTRS IFFDAVEGSF APVMINGAMA DAVRGRSISR 310 320 330 340 350 360 EANTGYDLGA SAIEAILLLA RAMDPATAAR WRGLCAGWIA RDTYRPILNS ASVPRTALVK 370 380 390 400 410 420 QLEATGVAPV AEATGHKLFP AMDRTMHRGP GWALSLALSS NRIAWYECGN GENNRGYHTG 430 440 450 460 470 480 SGMTYFYTSD LGQYDDAFWA TANYNRLPGI TVDTTPLPDK VEGQWGAAVP ADEWSGATAL 490 500 510 520 530 540 GEVAAVGQHL VGPGRTGLTA RKSWFVSGDV TVCLGADIST ASGAKVETIV DHRNLHQGSN 550 560 570 580 590 600 TLTTAAGTIA GTAGTVEVLG DGRWVHLEGF GGYAMLDDSP LHVLRETRSG SWSGVNINGS 610 620 630 640 650 660 ATVQQRNFAT LYVNHGVGPV AGSYAYMVAP GASVDLTRKL LEGNKYSVIR NDATAQSVEF 670 680 690 700 710 720 KTAKTTAATF WKPGMAGDLG ASGPACVVFS RHGNELSLAV SEPTQKAAGL TLTLPEGTWS 730 740 750 SVLEGAGTLG TDADGRSTLT LDTTGLSGKT KLIKLKR

References

[1]

"High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism." Lunin V.V., Li Y., Linhardt R.J., Miyazono H., Kyogashima M., Kaneko T., Bell A.W., Cygler M. J. Mol. Biol. 337:367-386(2004) [PubMed: 15003453] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RW9	X-ray	1.35	A	1-757	[»]
1RWA	X-ray	1.30	A	1-757	[»]
1RWC	X-ray	1.90	A	1-757	[»]
1RWF	X-ray	1.45	A	1-757	[»]
1RWG	X-ray	1.50	A	1-757	[»]
1RWH	X-ray	1.25	A	1-757	[»]

ProteinModelPortal

P84141.

SMR

P84141. Positions 4-757.

ModBase

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Protein family/group databases

CAZy

PL8. Polysaccharide Lyase Family 8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008929. Chondroitin_lyas.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
[Graphical view]

Gene3D

G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:2.60.220.10. Lyase_8_C. 1 hit.
G3DSA:1.50.10.100. Lyase_8_N. 1 hit.

Pfam

PF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view]

SUPFAM

SSF48230. Chondroitin_lyas. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49863. Lyase_like_C. 1 hit.

ProtoNet

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Entry information

Entry name

P84141_ARTAU

Accession

Primary (citable) accession number: P84141

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	July 27, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)