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P84140

- G6PI_THELI

UniProt

P84140 - G6PI_THELI

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Protein

Glucose-6-phosphate isomerase

Gene

pgiA

Organism
Thermococcus litoralis
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.1 Publication

Cofactori

Binds 1 iron ion per subunit.2 Publications

Enzyme regulationi

Inhibited by mannose 6-phosphate, fructose 1-phosphate and fructose 1,6-bisphosphate. Its activity is also inhibited by Cobalt (II) ions < EDTA < nickel (II) ions < zinc (II) ions << cadmium (II) ions < copper (II) ions. Sodium and potassium ions and manganese ions show little or no effect on activity.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Iron
Metal bindingi91 – 911Iron
Metal bindingi98 – 981Iron
Metal bindingi137 – 1371Iron

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: UniProtKB
  2. iron ion binding Source: UniProtKB

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB
  2. glycolytic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP84140.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgiA
OrganismiThermococcus litoralis
Taxonomic identifieri2265 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi89 – 891H → A: Loss of catalytic activity. 1 Publication
Mutagenesisi91 – 911H → A: Loss of catalytic activity. 1 Publication
Mutagenesisi98 – 981E → V: Loss of catalytic activity. 1 Publication
Mutagenesisi137 – 1371H → A: Loss of catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 190190Glucose-6-phosphate isomerasePRO_0000185360Add
BLAST

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1
190
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115
Turni13 – 153
Beta strandi22 – 276
Helixi28 – 303
Turni32 – 343
Helixi38 – 4710
Beta strandi51 – 588
Beta strandi67 – 748
Beta strandi98 – 1058
Beta strandi107 – 1126
Beta strandi118 – 1236
Beta strandi127 – 1315
Beta strandi136 – 1416
Beta strandi143 – 1453
Beta strandi147 – 1548
Helixi162 – 1676
Beta strandi170 – 18314

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J3PX-ray2.02A/B1-190[»]
1J3QX-ray1.85A/B1-190[»]
1J3RX-ray2.18A/B1-190[»]
ProteinModelPortaliP84140.
SMRiP84140. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84140.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal-type GPI family.Curated

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01410. G6P_isomerase_arch.
InterProiIPR010551. G6P_isomerase_prok.
IPR016758. G6P_isomerase_subgr.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF06560. GPI. 1 hit.
[Graphical view]
PIRSFiPIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P84140-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYKEPFGVK LDFETGIIEN AKKSVRRLSD MKGYFIDEEA WKKMVEEGDP
60 70 80 90 100
VVYEVYAIEQ EEKEGDLNFA TTVLYPGKVG NEFFMTKGHY HSKIDRAEVY
110 120 130 140 150
FALKGKGGML LQTPEGEARF IEMEPGTIVY VPPYWAHRTI NTGDKPFIFL
160 170 180 190
ALYPADAGHD YGTIAEKGFS KIVVEENGKV VVKDNPKWRM
Length:190
Mass (Da):21,720
Last modified:September 13, 2004 - v1
Checksum:i0D91C56E61DD7D3F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081724 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081724 Genomic DNA. No translation available.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J3P X-ray 2.02 A/B 1-190 [» ]
1J3Q X-ray 1.85 A/B 1-190 [» ]
1J3R X-ray 2.18 A/B 1-190 [» ]
ProteinModelPortali P84140.
SMRi P84140. Positions 1-187.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .
SABIO-RK P84140.

Miscellaneous databases

EvolutionaryTracei P84140.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_01410. G6P_isomerase_arch.
InterProi IPR010551. G6P_isomerase_prok.
IPR016758. G6P_isomerase_subgr.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF06560. GPI. 1 hit.
[Graphical view ]
PIRSFi PIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis."
    Jeong J.-J., Fushinobu S., Ito S., Jeon B.S., Shoun H., Wakagi T.
    FEBS Lett. 535:200-204(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBUNIT, MUTAGENESIS OF HIS-89; HIS-91; GLU-98 AND HIS-137.
  2. "Crystal structure of Thermococcus litoralis phosphoglucose isomerase."
    Jeong J.-J., Fushinobu S., Hidaka M., Shoun H., Wakagi T.
    Submitted (FEB-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS), COFACTOR, SUBUNIT.

Entry informationi

Entry nameiG6PI_THELI
AccessioniPrimary (citable) accession number: P84140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3