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P84104

- SRSF3_MOUSE

UniProt

P84104 - SRSF3_MOUSE

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Protein
Serine/arginine-rich splicing factor 3
Gene
Srsf3, Sfrs3, Srp20, X16
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May be involved in RNA processing in relation with cellular proliferation and/or maturation. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific By similarity.

GO - Molecular functioni

  1. RNA binding Source: BHF-UCL
  2. nucleotide binding Source: InterPro
  3. phospholipase binding Source: BHF-UCL
Complete GO annotation...

GO - Biological processi

  1. RNA splicing Source: BHF-UCL
  2. insulin receptor signaling pathway Source: BHF-UCL
  3. mRNA processing Source: UniProtKB-KW
  4. mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Protein X16
Splicing factor, arginine/serine-rich 3
Gene namesi
Name:Srsf3
Synonyms:Sfrs3, Srp20, X16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:98285. Srsf3.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nuclear speck Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 164164Serine/arginine-rich splicing factor 3
PRO_0000081924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei5 – 51Phosphoserine By similarity
Modified residuei23 – 231N6-acetyllysine By similarity

Post-translational modificationi

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP84104.
PaxDbiP84104.
PRIDEiP84104.

PTM databases

PhosphoSiteiP84104.

Expressioni

Tissue specificityi

Highest expression in thymus and pre-B cell lines; high, in testis, brain and spleen; very low in heart and not detectable in liver and kidney.1 Publication

Inductioni

Isoform long is induced by serum; in a tissue culture.1 Publication

Gene expression databases

ArrayExpressiP84104.
BgeeiP84104.
CleanExiMM_SFRS3.
GenevestigatoriP84104.

Interactioni

Subunit structurei

Interacts with CPSF6, RBMY1A1 and SREK1/SFRS12 By similarity.

Protein-protein interaction databases

BioGridi203189. 12 interactions.
IntActiP84104. 4 interactions.
MINTiMINT-4134334.

Structurei

3D structure databases

ProteinModelPortaliP84104.
SMRiP84104. Positions 3-86.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 8374RRM
Add
BLAST
Repeati119 – 13315B-1
Add
BLAST
Repeati149 – 16416B-2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9090Sufficiernt for interaction with NXF1 By similarity
Add
BLAST
Regioni119 – 164462 X approximate repeats, basic
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi86 – 16479Arg/Ser-rich (RS domain)
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG302928.
GeneTreeiENSGT00700000104452.
HOVERGENiHBG107480.
InParanoidiP84104.
KOiK12892.
OMAiYSPAARS.
OrthoDBiEOG73NG5V.
PhylomeDBiP84104.
TreeFamiTF351858.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P84104-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF    50
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP 100
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS 150
FSRSRSRSRS NERK 164
Length:164
Mass (Da):19,330
Last modified:August 16, 2004 - v1
Checksum:i02F0A5EE33FF28A0
GO
Isoform Short (identifier: P84104-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-124: SPRRRSFSRS → VTIMSLLTTL
     125-164: Missing.

Show »
Length:124
Mass (Da):14,203
Checksum:i78979851C38232C1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei115 – 12410SPRRRSFSRS → VTIMSLLTTL in isoform Short.
VSP_005861
Alternative sequencei125 – 16440Missing in isoform Short.
VSP_005862Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53824 mRNA. Translation: CAA37821.1.
X91656 Genomic DNA. Translation: CAA62844.1.
X91656 Genomic DNA. Translation: CAA62845.1.
AK011657 mRNA. Translation: BAB27762.1.
AK078891 mRNA. Translation: BAC37445.1.
AK134177 mRNA. Translation: BAE22040.1.
AK135298 mRNA. Translation: BAE22483.1.
AK145089 mRNA. Translation: BAE26230.1.
AK145715 mRNA. Translation: BAE26606.1.
AK146010 mRNA. Translation: BAE26830.1.
AK146253 mRNA. Translation: BAE27015.1.
AK150912 mRNA. Translation: BAE29950.1.
AK153209 mRNA. Translation: BAE31808.1.
BC068111 mRNA. Translation: AAH68111.1.
BC071196 mRNA. Translation: AAH71196.1.
BC083316 mRNA. Translation: AAH83316.1.
CCDSiCCDS28590.1.
PIRiS14016.
RefSeqiNP_038691.1. NM_013663.5. [P84104-1]
UniGeneiMm.473125.
Mm.6787.

Genome annotation databases

EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172. [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172. [P84104-1]
GeneIDi20383.
KEGGimmu:20383.
UCSCiuc008bsd.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53824 mRNA. Translation: CAA37821.1 .
X91656 Genomic DNA. Translation: CAA62844.1 .
X91656 Genomic DNA. Translation: CAA62845.1 .
AK011657 mRNA. Translation: BAB27762.1 .
AK078891 mRNA. Translation: BAC37445.1 .
AK134177 mRNA. Translation: BAE22040.1 .
AK135298 mRNA. Translation: BAE22483.1 .
AK145089 mRNA. Translation: BAE26230.1 .
AK145715 mRNA. Translation: BAE26606.1 .
AK146010 mRNA. Translation: BAE26830.1 .
AK146253 mRNA. Translation: BAE27015.1 .
AK150912 mRNA. Translation: BAE29950.1 .
AK153209 mRNA. Translation: BAE31808.1 .
BC068111 mRNA. Translation: AAH68111.1 .
BC071196 mRNA. Translation: AAH71196.1 .
BC083316 mRNA. Translation: AAH83316.1 .
CCDSi CCDS28590.1.
PIRi S14016.
RefSeqi NP_038691.1. NM_013663.5. [P84104-1 ]
UniGenei Mm.473125.
Mm.6787.

3D structure databases

ProteinModelPortali P84104.
SMRi P84104. Positions 3-86.
ModBasei Search...

Protein-protein interaction databases

BioGridi 203189. 12 interactions.
IntActi P84104. 4 interactions.
MINTi MINT-4134334.

PTM databases

PhosphoSitei P84104.

Proteomic databases

MaxQBi P84104.
PaxDbi P84104.
PRIDEi P84104.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037776 ; ENSMUSP00000049025 ; ENSMUSG00000071172 . [P84104-2 ]
ENSMUST00000130216 ; ENSMUSP00000117045 ; ENSMUSG00000071172 . [P84104-1 ]
GeneIDi 20383.
KEGGi mmu:20383.
UCSCi uc008bsd.2. mouse.

Organism-specific databases

CTDi 6428.
MGIi MGI:98285. Srsf3.

Phylogenomic databases

eggNOGi NOG302928.
GeneTreei ENSGT00700000104452.
HOVERGENi HBG107480.
InParanoidi P84104.
KOi K12892.
OMAi YSPAARS.
OrthoDBi EOG73NG5V.
PhylomeDBi P84104.
TreeFami TF351858.

Miscellaneous databases

NextBioi 24965.
PROi P84104.
SOURCEi Search...

Gene expression databases

ArrayExpressi P84104.
Bgeei P84104.
CleanExi MM_SFRS3.
Genevestigatori P84104.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs."
    Ayane M., Preuss U., Koehler G., Nielsen P.J.
    Nucleic Acids Res. 19:1273-1278(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INDUCTION, TISSUE SPECIFICITY.
  2. "Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle."
    Jumaa H., Guenet J.-L., Nielsen P.J.
    Mol. Cell. Biol. 17:3116-3124(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: MRL.
    Tissue: Lymphoid tissue.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: C57BL/6J and DBA/2.
    Tissue: Bone marrow, Colon, Mammary gland, Placenta, Thymus and Wolffian duct.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: C57BL/6 and C57BL/6J.
    Tissue: Brain and Embryo.
  5. "Characterization and comparison of four serine- and arginine-rich (SR) protein kinases."
    Nayler O., Stamm S., Ullrich A.
    Biochem. J. 326:693-700(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY CLK1; CLK2; CLK3 AND CLK4.

Entry informationi

Entry nameiSRSF3_MOUSE
AccessioniPrimary (citable) accession number: P84104
Secondary accession number(s): O08831, P23152, Q564E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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