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P84104

- SRSF3_MOUSE

UniProt

P84104 - SRSF3_MOUSE

Protein

Serine/arginine-rich splicing factor 3

Gene

Srsf3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    May be involved in RNA processing in relation with cellular proliferation and/or maturation. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. phospholipase binding Source: BHF-UCL
    3. RNA binding Source: BHF-UCL

    GO - Biological processi

    1. insulin receptor signaling pathway Source: BHF-UCL
    2. mRNA processing Source: UniProtKB-KW
    3. mRNA transport Source: UniProtKB-KW
    4. RNA splicing Source: BHF-UCL

    Keywords - Biological processi

    mRNA processing, mRNA splicing, mRNA transport, Transport

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/arginine-rich splicing factor 3
    Alternative name(s):
    Pre-mRNA-splicing factor SRP20
    Protein X16
    Splicing factor, arginine/serine-rich 3
    Gene namesi
    Name:Srsf3
    Synonyms:Sfrs3, Srp20, X16
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98285. Srsf3.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear speck Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 164164Serine/arginine-rich splicing factor 3PRO_0000081924Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei5 – 51PhosphoserineBy similarity
    Modified residuei23 – 231N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP84104.
    PaxDbiP84104.
    PRIDEiP84104.

    PTM databases

    PhosphoSiteiP84104.

    Expressioni

    Tissue specificityi

    Highest expression in thymus and pre-B cell lines; high, in testis, brain and spleen; very low in heart and not detectable in liver and kidney.1 Publication

    Inductioni

    Isoform long is induced by serum; in a tissue culture.1 Publication

    Gene expression databases

    ArrayExpressiP84104.
    BgeeiP84104.
    CleanExiMM_SFRS3.
    GenevestigatoriP84104.

    Interactioni

    Subunit structurei

    Interacts with CPSF6, RBMY1A1 and SREK1/SFRS12.By similarity

    Protein-protein interaction databases

    BioGridi203189. 12 interactions.
    IntActiP84104. 4 interactions.
    MINTiMINT-4134334.

    Structurei

    3D structure databases

    ProteinModelPortaliP84104.
    SMRiP84104. Positions 3-86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 8374RRMPROSITE-ProRule annotationAdd
    BLAST
    Repeati119 – 13315B-1Add
    BLAST
    Repeati149 – 16416B-2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9090Sufficiernt for interaction with NXF1By similarityAdd
    BLAST
    Regioni119 – 164462 X approximate repeats, basicAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi86 – 16479Arg/Ser-rich (RS domain)Add
    BLAST

    Sequence similaritiesi

    Belongs to the splicing factor SR family.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG302928.
    GeneTreeiENSGT00700000104452.
    HOVERGENiHBG107480.
    InParanoidiP84104.
    KOiK12892.
    OMAiYSPAARS.
    OrthoDBiEOG73NG5V.
    PhylomeDBiP84104.
    TreeFamiTF351858.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P84104-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF    50
    VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP 100
    RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS 150
    FSRSRSRSRS NERK 164
    Length:164
    Mass (Da):19,330
    Last modified:August 16, 2004 - v1
    Checksum:i02F0A5EE33FF28A0
    GO
    Isoform Short (identifier: P84104-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         115-124: SPRRRSFSRS → VTIMSLLTTL
         125-164: Missing.

    Show »
    Length:124
    Mass (Da):14,203
    Checksum:i78979851C38232C1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei115 – 12410SPRRRSFSRS → VTIMSLLTTL in isoform Short. CuratedVSP_005861
    Alternative sequencei125 – 16440Missing in isoform Short. CuratedVSP_005862Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53824 mRNA. Translation: CAA37821.1.
    X91656 Genomic DNA. Translation: CAA62844.1.
    X91656 Genomic DNA. Translation: CAA62845.1.
    AK011657 mRNA. Translation: BAB27762.1.
    AK078891 mRNA. Translation: BAC37445.1.
    AK134177 mRNA. Translation: BAE22040.1.
    AK135298 mRNA. Translation: BAE22483.1.
    AK145089 mRNA. Translation: BAE26230.1.
    AK145715 mRNA. Translation: BAE26606.1.
    AK146010 mRNA. Translation: BAE26830.1.
    AK146253 mRNA. Translation: BAE27015.1.
    AK150912 mRNA. Translation: BAE29950.1.
    AK153209 mRNA. Translation: BAE31808.1.
    BC068111 mRNA. Translation: AAH68111.1.
    BC071196 mRNA. Translation: AAH71196.1.
    BC083316 mRNA. Translation: AAH83316.1.
    CCDSiCCDS28590.1.
    PIRiS14016.
    RefSeqiNP_038691.1. NM_013663.5. [P84104-1]
    UniGeneiMm.473125.
    Mm.6787.

    Genome annotation databases

    EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172. [P84104-2]
    ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172. [P84104-1]
    GeneIDi20383.
    KEGGimmu:20383.
    UCSCiuc008bsd.2. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53824 mRNA. Translation: CAA37821.1 .
    X91656 Genomic DNA. Translation: CAA62844.1 .
    X91656 Genomic DNA. Translation: CAA62845.1 .
    AK011657 mRNA. Translation: BAB27762.1 .
    AK078891 mRNA. Translation: BAC37445.1 .
    AK134177 mRNA. Translation: BAE22040.1 .
    AK135298 mRNA. Translation: BAE22483.1 .
    AK145089 mRNA. Translation: BAE26230.1 .
    AK145715 mRNA. Translation: BAE26606.1 .
    AK146010 mRNA. Translation: BAE26830.1 .
    AK146253 mRNA. Translation: BAE27015.1 .
    AK150912 mRNA. Translation: BAE29950.1 .
    AK153209 mRNA. Translation: BAE31808.1 .
    BC068111 mRNA. Translation: AAH68111.1 .
    BC071196 mRNA. Translation: AAH71196.1 .
    BC083316 mRNA. Translation: AAH83316.1 .
    CCDSi CCDS28590.1.
    PIRi S14016.
    RefSeqi NP_038691.1. NM_013663.5. [P84104-1 ]
    UniGenei Mm.473125.
    Mm.6787.

    3D structure databases

    ProteinModelPortali P84104.
    SMRi P84104. Positions 3-86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203189. 12 interactions.
    IntActi P84104. 4 interactions.
    MINTi MINT-4134334.

    PTM databases

    PhosphoSitei P84104.

    Proteomic databases

    MaxQBi P84104.
    PaxDbi P84104.
    PRIDEi P84104.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037776 ; ENSMUSP00000049025 ; ENSMUSG00000071172 . [P84104-2 ]
    ENSMUST00000130216 ; ENSMUSP00000117045 ; ENSMUSG00000071172 . [P84104-1 ]
    GeneIDi 20383.
    KEGGi mmu:20383.
    UCSCi uc008bsd.2. mouse.

    Organism-specific databases

    CTDi 6428.
    MGIi MGI:98285. Srsf3.

    Phylogenomic databases

    eggNOGi NOG302928.
    GeneTreei ENSGT00700000104452.
    HOVERGENi HBG107480.
    InParanoidi P84104.
    KOi K12892.
    OMAi YSPAARS.
    OrthoDBi EOG73NG5V.
    PhylomeDBi P84104.
    TreeFami TF351858.

    Miscellaneous databases

    NextBioi 24965.
    PROi P84104.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P84104.
    Bgeei P84104.
    CleanExi MM_SFRS3.
    Genevestigatori P84104.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs."
      Ayane M., Preuss U., Koehler G., Nielsen P.J.
      Nucleic Acids Res. 19:1273-1278(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INDUCTION, TISSUE SPECIFICITY.
    2. "Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle."
      Jumaa H., Guenet J.-L., Nielsen P.J.
      Mol. Cell. Biol. 17:3116-3124(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: MRL.
      Tissue: Lymphoid tissue.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: C57BL/6J and DBA/2.
      Tissue: Bone marrow, Colon, Mammary gland, Placenta, Thymus and Wolffian duct.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: C57BL/6 and C57BL/6J.
      Tissue: Brain and Embryo.
    5. "Characterization and comparison of four serine- and arginine-rich (SR) protein kinases."
      Nayler O., Stamm S., Ullrich A.
      Biochem. J. 326:693-700(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY CLK1; CLK2; CLK3 AND CLK4.

    Entry informationi

    Entry nameiSRSF3_MOUSE
    AccessioniPrimary (citable) accession number: P84104
    Secondary accession number(s): O08831, P23152, Q564E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3