Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/arginine-rich splicing factor 3

Gene

Srsf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.By similarity

GO - Molecular functioni

GO - Biological processi

  • insulin receptor signaling pathway Source: BHF-UCL
  • mRNA processing Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Protein X16
Splicing factor, arginine/serine-rich 3
Gene namesi
Name:Srsf3
Synonyms:Sfrs3, Srp20, X16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98285. Srsf3.

Subcellular locationi

  • Nucleus By similarity
  • Nucleus speckle By similarity
  • Cytoplasm By similarity

  • Note: Recruited to nuclear speckles following interaction with YTHDC1.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear speck Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819241 – 164Serine/arginine-rich splicing factor 3Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei23N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP84104.
PaxDbiP84104.
PeptideAtlasiP84104.
PRIDEiP84104.

PTM databases

iPTMnetiP84104.
PhosphoSitePlusiP84104.

Expressioni

Tissue specificityi

Highest expression in thymus and pre-B cell lines; high, in testis, brain and spleen; very low in heart and not detectable in liver and kidney.1 Publication

Inductioni

Isoform long is induced by serum; in a tissue culture.1 Publication

Gene expression databases

BgeeiENSMUSG00000071172.
CleanExiMM_SFRS3.
ExpressionAtlasiP84104. baseline and differential.
GenevisibleiP84104. MM.

Interactioni

Subunit structurei

Interacts with CPSF6. Interacts with RBMY1A1. Interacts with SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites.By similarity

GO - Molecular functioni

  • phospholipase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi203189. 8 interactors.
IntActiP84104. 4 interactors.
MINTiMINT-4134334.
STRINGi10090.ENSMUSP00000117045.

Structurei

3D structure databases

ProteinModelPortaliP84104.
SMRiP84104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83RRMPROSITE-ProRule annotationAdd BLAST74
Repeati119 – 133B-1Add BLAST15
Repeati149 – 164B-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Sufficient for interaction with NXF1By similarityAdd BLAST90
Regioni119 – 1642 X approximate repeats, basicAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 164Arg/Ser-rich (RS domain)Add BLAST79

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG107480.
InParanoidiP84104.
KOiK12892.
OMAiDSIMHRD.
OrthoDBiEOG091G17YQ.
PhylomeDBiP84104.
TreeFamiTF351858.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P84104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF
60 70 80 90 100
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP
110 120 130 140 150
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS
160
FSRSRSRSRS NERK
Length:164
Mass (Da):19,330
Last modified:August 16, 2004 - v1
Checksum:i02F0A5EE33FF28A0
GO
Isoform Short (identifier: P84104-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-124: SPRRRSFSRS → VTIMSLLTTL
     125-164: Missing.

Show »
Length:124
Mass (Da):14,203
Checksum:i78979851C38232C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005861115 – 124SPRRRSFSRS → VTIMSLLTTL in isoform Short. Curated10
Alternative sequenceiVSP_005862125 – 164Missing in isoform Short. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53824 mRNA. Translation: CAA37821.1.
X91656 Genomic DNA. Translation: CAA62844.1.
X91656 Genomic DNA. Translation: CAA62845.1.
AK011657 mRNA. Translation: BAB27762.1.
AK078891 mRNA. Translation: BAC37445.1.
AK134177 mRNA. Translation: BAE22040.1.
AK135298 mRNA. Translation: BAE22483.1.
AK145089 mRNA. Translation: BAE26230.1.
AK145715 mRNA. Translation: BAE26606.1.
AK146010 mRNA. Translation: BAE26830.1.
AK146253 mRNA. Translation: BAE27015.1.
AK150912 mRNA. Translation: BAE29950.1.
AK153209 mRNA. Translation: BAE31808.1.
BC068111 mRNA. Translation: AAH68111.1.
BC071196 mRNA. Translation: AAH71196.1.
BC083316 mRNA. Translation: AAH83316.1.
CCDSiCCDS28590.1.
PIRiS14016.
RefSeqiNP_038691.1. NM_013663.5. [P84104-1]
UniGeneiMm.473125.
Mm.486331.
Mm.6787.

Genome annotation databases

EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172. [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172. [P84104-1]
GeneIDi20383.
KEGGimmu:20383.
UCSCiuc008bsd.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53824 mRNA. Translation: CAA37821.1.
X91656 Genomic DNA. Translation: CAA62844.1.
X91656 Genomic DNA. Translation: CAA62845.1.
AK011657 mRNA. Translation: BAB27762.1.
AK078891 mRNA. Translation: BAC37445.1.
AK134177 mRNA. Translation: BAE22040.1.
AK135298 mRNA. Translation: BAE22483.1.
AK145089 mRNA. Translation: BAE26230.1.
AK145715 mRNA. Translation: BAE26606.1.
AK146010 mRNA. Translation: BAE26830.1.
AK146253 mRNA. Translation: BAE27015.1.
AK150912 mRNA. Translation: BAE29950.1.
AK153209 mRNA. Translation: BAE31808.1.
BC068111 mRNA. Translation: AAH68111.1.
BC071196 mRNA. Translation: AAH71196.1.
BC083316 mRNA. Translation: AAH83316.1.
CCDSiCCDS28590.1.
PIRiS14016.
RefSeqiNP_038691.1. NM_013663.5. [P84104-1]
UniGeneiMm.473125.
Mm.486331.
Mm.6787.

3D structure databases

ProteinModelPortaliP84104.
SMRiP84104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203189. 8 interactors.
IntActiP84104. 4 interactors.
MINTiMINT-4134334.
STRINGi10090.ENSMUSP00000117045.

PTM databases

iPTMnetiP84104.
PhosphoSitePlusiP84104.

Proteomic databases

MaxQBiP84104.
PaxDbiP84104.
PeptideAtlasiP84104.
PRIDEiP84104.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172. [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172. [P84104-1]
GeneIDi20383.
KEGGimmu:20383.
UCSCiuc008bsd.2. mouse.

Organism-specific databases

CTDi6428.
MGIiMGI:98285. Srsf3.

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG107480.
InParanoidiP84104.
KOiK12892.
OMAiDSIMHRD.
OrthoDBiEOG091G17YQ.
PhylomeDBiP84104.
TreeFamiTF351858.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiP84104.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071172.
CleanExiMM_SFRS3.
ExpressionAtlasiP84104. baseline and differential.
GenevisibleiP84104. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF3_MOUSE
AccessioniPrimary (citable) accession number: P84104
Secondary accession number(s): O08831, P23152, Q564E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.