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P84104 (SRSF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Protein X16
Splicing factor, arginine/serine-rich 3
Gene names
Name:Srsf3
Synonyms:Sfrs3, Srp20, X16
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length164 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in RNA processing in relation with cellular proliferation and/or maturation. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific By similarity.

Subunit structure

Interacts with CPSF6, RBMY1A1 and SREK1/SFRS12 By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity.

Tissue specificity

Highest expression in thymus and pre-B cell lines; high, in testis, brain and spleen; very low in heart and not detectable in liver and kidney. Ref.1

Induction

Isoform longis induced by serum; in a tissue culture. Ref.1

Post-translational modification

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain. Ref.5

Sequence similarities

Belongs to the splicing factor SR family.

Contains 1 RRM (RNA recognition motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P84104-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P84104-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-124: SPRRRSFSRS → VTIMSLLTTL
     125-164: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 164164Serine/arginine-rich splicing factor 3
PRO_0000081924

Regions

Domain10 – 8374RRM
Repeat119 – 13315B-1
Repeat149 – 16416B-2
Region1 – 9090Sufficiernt for interaction with NXF1 By similarity
Region119 – 164462 X approximate repeats, basic
Compositional bias86 – 16479Arg/Ser-rich (RS domain)

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue51Phosphoserine By similarity
Modified residue231N6-acetyllysine By similarity

Natural variations

Alternative sequence115 – 12410SPRRRSFSRS → VTIMSLLTTL in isoform Short.
VSP_005861
Alternative sequence125 – 16440Missing in isoform Short.
VSP_005862

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 02F0A5EE33FF28A0

FASTA16419,330
        10         20         30         40         50         60 
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF VEFEDPRDAA 

        70         80         90        100        110        120 
DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP RDDYRRRSPP PRRRSPRRRS 

       130        140        150        160 
FSRSRSRSLS RDRRRERSLS RERNHKPSRS FSRSRSRSRS NERK 

« Hide

Isoform Short [UniParc] [UniParc].

Checksum: 78979851C38232C1
Show »

FASTA12414,203

References

« Hide 'large scale' references
[1]"A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs."
Ayane M., Preuss U., Koehler G., Nielsen P.J.
Nucleic Acids Res. 19:1273-1278(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INDUCTION, TISSUE SPECIFICITY.
[2]"Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle."
Jumaa H., Guenet J.-L., Nielsen P.J.
Mol. Cell. Biol. 17:3116-3124(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: MRL.
Tissue: Lymphoid tissue.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: C57BL/6J and DBA/2.
Tissue: Bone marrow, Colon, Mammary gland, Placenta, Thymus and Wolffian duct.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: C57BL/6 and C57BL/6J.
Tissue: Brain and Embryo.
[5]"Characterization and comparison of four serine- and arginine-rich (SR) protein kinases."
Nayler O., Stamm S., Ullrich A.
Biochem. J. 326:693-700(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CLK1; CLK2; CLK3 AND CLK4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53824 mRNA. Translation: CAA37821.1.
X91656 Genomic DNA. Translation: CAA62844.1.
X91656 Genomic DNA. Translation: CAA62845.1.
AK011657 mRNA. Translation: BAB27762.1.
AK078891 mRNA. Translation: BAC37445.1.
AK134177 mRNA. Translation: BAE22040.1.
AK135298 mRNA. Translation: BAE22483.1.
AK145089 mRNA. Translation: BAE26230.1.
AK145715 mRNA. Translation: BAE26606.1.
AK146010 mRNA. Translation: BAE26830.1.
AK146253 mRNA. Translation: BAE27015.1.
AK150912 mRNA. Translation: BAE29950.1.
AK153209 mRNA. Translation: BAE31808.1.
BC068111 mRNA. Translation: AAH68111.1.
BC071196 mRNA. Translation: AAH71196.1.
BC083316 mRNA. Translation: AAH83316.1.
PIRS14016.
RefSeqNP_038691.1. NM_013663.5.
UniGeneMm.473125.
Mm.6787.

3D structure databases

ProteinModelPortalP84104.
SMRP84104. Positions 3-86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203189. 11 interactions.
IntActP84104. 4 interactions.
MINTMINT-4134334.

PTM databases

PhosphoSiteP84104.

Proteomic databases

PaxDbP84104.
PRIDEP84104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037776; ENSMUSP00000049025; ENSMUSG00000071172. [P84104-2]
ENSMUST00000130216; ENSMUSP00000117045; ENSMUSG00000071172. [P84104-1]
GeneID20383.
KEGGmmu:20383.
UCSCuc008bsd.2. mouse.

Organism-specific databases

CTD6428.
MGIMGI:98285. Srsf3.

Phylogenomic databases

eggNOGNOG302928.
GeneTreeENSGT00700000104452.
HOVERGENHBG107480.
InParanoidP84104.
KOK12892.
OMASPPPRRX.
OrthoDBEOG73NG5V.
PhylomeDBP84104.
TreeFamTF351858.

Gene expression databases

ArrayExpressP84104.
BgeeP84104.
CleanExMM_SFRS3.
GenevestigatorP84104.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio24965.
PROP84104.
SOURCESearch...

Entry information

Entry nameSRSF3_MOUSE
AccessionPrimary (citable) accession number: P84104
Secondary accession number(s): O08831, P23152, Q564E9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot