Reviewed, UniProtKB/Swiss-Prot P84103 (SFRS3_HUMAN)

Names and origin

Protein names

Recommended name:
    Splicing factor, arginine/serine-rich 3
Alternative name(s):
    Pre-mRNA-splicing factor SRP20

Gene names

Name:	SFRS3
Synonyms:	SRP20

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in RNA processing in relation with cellular proliferation and/or maturation.
Subunit structure	Interacts with CPSF6, RBMY1A1 and SFRS12. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus.
Post-translational modification	Extensively phosphorylated on serine residues in the RS domain By similarity.
Sequence similarities	Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from Experiment. Source: Reactome
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Traceable author statement. Source: ProtInc nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Ref.8 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAQ	P27348	1	EBI-372557,EBI-359854

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 164

164

Splicing factor, arginine/serine-rich 3

PRO_0000081923

Regions

Domain

10 – 83

74

RRM

Repeat

119 – 133

15

B-1

Repeat

149 – 164

16

B-2

Region

119 – 164

46

2 X approximate repeats, basic

Compositional bias

86 – 164

79

Arg/Ser-rich (RS domain)

Amino acid modifications

Modified residue

81

1

Phosphoserine Ref.9

Modified residue

95

1

Phosphoserine Ref.10 Ref.11

Modified residue

108

1

Phosphoserine Ref.9

Modified residue

138

1

Phosphoserine Ref.9

Modified residue

140

1

Phosphoserine Ref.9

Modified residue

148

1

Phosphoserine Ref.9

Modified residue

150

1

Phosphoserine Ref.9

Modified residue

152

1

Phosphoserine Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

164

Helix Strand Turn

Details...

Beta strand

2 – 15

14

Turn

18 – 20

3

Helix

23 – 31

9

Beta strand

37 – 45

9

Beta strand

48 – 52

5

Helix

56 – 65

10

Beta strand

67 – 69

3

Beta strand

77 – 80

4

Sequences

	Sequence		Length	Mass (Da)
	P84103-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 02F0A5EE33FF28A0	FASTA	164	19,330
	10 20 30 40 50 60 MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF VEFEDPRDAA 70 80 90 100 110 120 DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP RDDYRRRSPP PRRRSPRRRS 130 140 150 160 FSRSRSRSLS RDRRRERSLS RERNHKPSRS FSRSRSRSRS NERK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SR proteins: a conserved family of pre-mRNA splicing factors." Zahler A.M., Lane W.S., Stolk J.A., Roth M.B. Genes Dev. 6:837-847(1992) [PubMed: 1577277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-37 AND 54-64.
[2]	"Identification and characterization of novel full-length cDNAs differentially expressed in hematopoietic lineages." Liu W.L., Wang M., Tang D., Rodgers G. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Placenta.
[6]	"Identification and characterization of a novel serine-arginine-rich splicing regulatory protein." Barnard D.C., Patton J.G. Mol. Cell. Biol. 20:3049-3057(2000) [PubMed: 10757789] [Abstract] Cited for: INTERACTION WITH SFRS12.
[7]	"The role of potential splicing factors including RBMY, RBMX, hnRNPG-T and STAR proteins in spermatogenesis." Elliott D.J. Int. J. Androl. 27:328-334(2004) [PubMed: 15595951] [Abstract] Cited for: INTERACTION WITH RBMY1A1.
[8]	"Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization." Dettwiler S., Aringhieri C., Cardinale S., Keller W., Barabino S.M. J. Biol. Chem. 279:35788-35797(2004) [PubMed: 15169763] [Abstract] Cited for: INTERACTION WITH CPSF6.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; SER-108; SER-138; SER-140; SER-148; SER-150 AND SER-152, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, MASS SPECTROMETRY.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L10838 mRNA. Translation: AAA36648.1.
AF107405 mRNA. Translation: AAD44523.1.
BT007017 mRNA. Translation: AAP35663.1.
Z85986 Genomic DNA. Translation: CAI19115.1.
BC000914 mRNA. Translation: AAH00914.1.
BC069018 mRNA. Translation: AAH69018.1.

IPI

IPI00010204.

PIR

I54089.

RefSeq

NP_003008.1.

UniGene

Hs.405144

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I2Y	NMR	-	A	1-86	[»]
2I38	NMR	-	A	1-86	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P84103. 6 interactions.

PTM databases

PhosphoSite

P84103.

Proteomic databases

PRIDE

P84103.

Genome annotation databases

Ensembl

ENSG00000112081. Homo sapiens. [Contig view]

GeneID

6428.

KEGG

hsa:6428.

UCSC

uc003omj.1. human.

Organism-specific databases

GeneCards

GC06P036670.

H-InvDB

HIX0005822.

HGNC

HGNC:10785. SFRS3.

HPA

CAB012986.

MIM

603364. gene.

PharmGKB

PA35701.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P84103.

HOVERGEN

P84103.

OMA

P84103. HEIESAF.

Enzyme and pathway databases

Reactome

REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1788. Transcription.
REACT_6167. Influenza Infection.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P84103.

Bgee

P84103.

CleanEx

HS_SFRS3.

GermOnline

ENSG00000112081. Homo sapiens.

Family and domain databases

InterPro

IPR012677. a_b_plait_nuc_bd.
IPR000504. RRM_RNP1.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.

Pfam

PF00076. RRM_1. 1 hit.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

24965.

SOURCE

Search...

Entry information

Entry name

SFRS3_HUMAN

Accession

Primary (citable) accession number: P84103
Secondary accession number(s): O08831, P23152, Q5R3K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	July 7, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)