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Protein

Serine/arginine-rich splicing factor 3

Gene

SRSF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor that specifically promotes exon-inclusion during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). Also functions as export adapter involved in mRNA nuclear export such as of histone H2A (PubMed:11336712, PubMed:18364396). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). RNA-binding is semi-sequence specific (PubMed:17036044).4 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • sequence-specific mRNA binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112081-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiP84103.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 3
Alternative name(s):
Pre-mRNA-splicing factor SRP20
Splicing factor, arginine/serine-rich 3
Gene namesi
Name:SRSF3
Synonyms:SFRS3, SRP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:10785. SRSF3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86R → E: Abolishes interaction with NXF1. 1 Publication1
Mutagenesisi88R → E: Abolishes interaction with NXF1. 1 Publication1
Mutagenesisi90R → E: Abolishes interaction with NXF1. 1 Publication1

Organism-specific databases

DisGeNETi6428.
OpenTargetsiENSG00000112081.
PharmGKBiPA35701.

Polymorphism and mutation databases

BioMutaiSRSF3.
DMDMi51338672.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819231 – 164Serine/arginine-rich splicing factor 3Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei23N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP84103.
MaxQBiP84103.
PaxDbiP84103.
PeptideAtlasiP84103.
PRIDEiP84103.
TopDownProteomicsiP84103-1. [P84103-1]

PTM databases

iPTMnetiP84103.
PhosphoSitePlusiP84103.
SwissPalmiP84103.

Expressioni

Gene expression databases

BgeeiENSG00000112081.
CleanExiHS_SFRS3.
ExpressionAtlasiP84103. baseline and differential.
GenevisibleiP84103. HS.

Organism-specific databases

HPAiCAB012986.
HPA056981.

Interactioni

Subunit structurei

Interacts with CPSF6 (PubMed:15169763). Interacts with RBMY1A1 (PubMed:15595951). Interacts with SREK1/SFRS12 (PubMed:10757789). Interacts with NXF1 (PubMed:12667464, PubMed:17036044). Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites (PubMed:26876937).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NXF1Q9UBU94EBI-372557,EBI-398874
PCBP2Q153663EBI-372557,EBI-945799
Srek1Q9JKL73EBI-372557,EBI-6452221From a different organism.

Protein-protein interaction databases

BioGridi112326. 126 interactors.
DIPiDIP-32961N.
IntActiP84103. 54 interactors.
MINTiMINT-5002707.
STRINGi9606.ENSP00000362820.

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi11 – 16Combined sources6
Turni18 – 20Combined sources3
Helixi24 – 33Combined sources10
Beta strandi36 – 41Combined sources6
Beta strandi43 – 45Combined sources3
Beta strandi47 – 55Combined sources9
Helixi56 – 66Combined sources11
Beta strandi67 – 69Combined sources3
Beta strandi71 – 74Combined sources4
Beta strandi77 – 80Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I2YNMR-A1-86[»]
2I38NMR-A1-86[»]
ProteinModelPortaliP84103.
SMRiP84103.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84103.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83RRMPROSITE-ProRule annotationAdd BLAST74
Repeati119 – 133B-1Add BLAST15
Repeati149 – 164B-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Sufficient for interaction with NXF1Add BLAST90
Regioni119 – 1642 X approximate repeats, basicAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 164Arg/Ser-rich (RS domain)Add BLAST79

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000208722.
HOVERGENiHBG107480.
InParanoidiP84103.
KOiK12892.
OMAiDSIMHRD.
OrthoDBiEOG091G17YQ.
PhylomeDBiP84103.
TreeFamiTF351858.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P84103-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF
60 70 80 90 100
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP
110 120 130 140 150
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS
160
FSRSRSRSRS NERK
Length:164
Mass (Da):19,330
Last modified:August 16, 2004 - v1
Checksum:i02F0A5EE33FF28A0
GO
Isoform 2 (identifier: P84103-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-164: SPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK → VTIMSLLTTL

Note: No experimental confirmation available.
Show »
Length:124
Mass (Da):14,203
Checksum:i78979851C38232C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056243115 – 164SPRRR…SNERK → VTIMSLLTTL in isoform 2. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10838 mRNA. Translation: AAA36648.1.
AF107405 mRNA. Translation: AAD44523.1.
AK304097 mRNA. Translation: BAG65003.1.
BT007017 mRNA. Translation: AAP35663.1.
Z85986 Genomic DNA. Translation: CAI19115.1.
CH471081 Genomic DNA. Translation: EAX03898.1.
BC000914 mRNA. Translation: AAH00914.1.
BC069018 mRNA. Translation: AAH69018.1.
CCDSiCCDS4823.1. [P84103-1]
PIRiI54089.
RefSeqiNP_003008.1. NM_003017.4. [P84103-1]
UniGeneiHs.405144.

Genome annotation databases

EnsembliENST00000373715; ENSP00000362820; ENSG00000112081. [P84103-1]
ENST00000477442; ENSP00000436036; ENSG00000112081. [P84103-2]
ENST00000620941; ENSP00000482833; ENSG00000112081. [P84103-1]
GeneIDi6428.
KEGGihsa:6428.
UCSCiuc003omj.4. human. [P84103-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10838 mRNA. Translation: AAA36648.1.
AF107405 mRNA. Translation: AAD44523.1.
AK304097 mRNA. Translation: BAG65003.1.
BT007017 mRNA. Translation: AAP35663.1.
Z85986 Genomic DNA. Translation: CAI19115.1.
CH471081 Genomic DNA. Translation: EAX03898.1.
BC000914 mRNA. Translation: AAH00914.1.
BC069018 mRNA. Translation: AAH69018.1.
CCDSiCCDS4823.1. [P84103-1]
PIRiI54089.
RefSeqiNP_003008.1. NM_003017.4. [P84103-1]
UniGeneiHs.405144.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I2YNMR-A1-86[»]
2I38NMR-A1-86[»]
ProteinModelPortaliP84103.
SMRiP84103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112326. 126 interactors.
DIPiDIP-32961N.
IntActiP84103. 54 interactors.
MINTiMINT-5002707.
STRINGi9606.ENSP00000362820.

PTM databases

iPTMnetiP84103.
PhosphoSitePlusiP84103.
SwissPalmiP84103.

Polymorphism and mutation databases

BioMutaiSRSF3.
DMDMi51338672.

Proteomic databases

EPDiP84103.
MaxQBiP84103.
PaxDbiP84103.
PeptideAtlasiP84103.
PRIDEiP84103.
TopDownProteomicsiP84103-1. [P84103-1]

Protocols and materials databases

DNASUi6428.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373715; ENSP00000362820; ENSG00000112081. [P84103-1]
ENST00000477442; ENSP00000436036; ENSG00000112081. [P84103-2]
ENST00000620941; ENSP00000482833; ENSG00000112081. [P84103-1]
GeneIDi6428.
KEGGihsa:6428.
UCSCiuc003omj.4. human. [P84103-1]

Organism-specific databases

CTDi6428.
DisGeNETi6428.
GeneCardsiSRSF3.
HGNCiHGNC:10785. SRSF3.
HPAiCAB012986.
HPA056981.
MIMi603364. gene.
neXtProtiNX_P84103.
OpenTargetsiENSG00000112081.
PharmGKBiPA35701.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000208722.
HOVERGENiHBG107480.
InParanoidiP84103.
KOiK12892.
OMAiDSIMHRD.
OrthoDBiEOG091G17YQ.
PhylomeDBiP84103.
TreeFamiTF351858.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112081-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiP84103.

Miscellaneous databases

ChiTaRSiSRSF3. human.
EvolutionaryTraceiP84103.
GeneWikiiSFRS3.
GenomeRNAii6428.
PROiP84103.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112081.
CleanExiHS_SFRS3.
ExpressionAtlasiP84103. baseline and differential.
GenevisibleiP84103. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF3_HUMAN
AccessioniPrimary (citable) accession number: P84103
Secondary accession number(s): B4E241
, O08831, P23152, Q5R3K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.