Reviewed, UniProtKB/Swiss-Prot P84095 (RHOG_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho-related GTP-binding protein RhoG

Gene names

Name:	RHOG
Synonyms:	ARHG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
Subunit structure	Interacts with SGEF.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	ADP-ribosylation Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.1 Traceable author statement. Source: ProtInc positive regulation of cell proliferation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ELMO1	Q92556	2	EBI-446579,EBI-346417

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Rho-related GTP-binding protein RhoG		PRO_0000042028
	Propeptide	189 – 191	3	Removed in mature form By similarity		PRO_0000042029
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	39	1	ADP-ribosylasparagine; by botulinum toxin By similarity
	Modified residue	188	1	Cysteine methyl ester By similarity
	Lipidation	188	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	133	1	G → S in CAA43784. Ref.1
	Sequence conflict	169	1	F → L in AAM21121. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P84095-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 0C4FE9C54140F499	FASTA	191	21,309
	10 20 30 40 50 60 MQSIKCVVVG DGAVGKTCLL ICYTTNAFPK EYIPTVFDNY SAQSAVDGRT VNLNLWDTAG 70 80 90 100 110 120 QEEYDRLRTL SYPQTNVFVI CFSIASPPSY ENVRHKWHPE VCHHCPDVPI LLVGTKKDLR 130 140 150 160 170 180 AQPDTLRRLK EQGQAPITPQ QGQALAKQIH AVRYLECSAL QQDGVKEVFA EAVRAVLNPT 190 PIKRGRSCIL L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Growth-regulated expression of rhoG, a new member of the ras homolog gene family." Vincent S., Jeanteur P., Fort P. Mol. Cell. Biol. 12:3138-3148(1992) [PubMed: 1620121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Oncogene ect2 is related to regulators of small GTP-binding proteins." Miki T., Smith C.L., Long J.E., Eva A., Fleming T.P. Nature 362:462-465(1993) [PubMed: 8464478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis." Ellerbroek S.M., Wennerberg K., Arthur W.T., Dunty J.M., Bowman D.R., DeMali K.A., Der C., Burridge K. Mol. Biol. Cell 15:3309-3319(2004) [PubMed: 15133129] [Abstract] Cited for: INTERACTION WITH SGEF, FUNCTION.
[5]	"Differential activation and function of Rho GTPases during Salmonella-host cell interactions." Patel J.C., Galan J.E. J. Cell Biol. 175:453-463(2006) [PubMed: 17074883] [Abstract] Cited for: FUNCTION.
[6]	"RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration." van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., Burridge K. J. Cell Biol. 178:1279-1293(2007) [PubMed: 17875742] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L11317 mRNA. Translation: AAA60268.1. X61587 mRNA. Translation: CAA43784.1. AF498974 mRNA. Translation: AAM21121.1. AY563952 mRNA. Translation: AAS75333.1.
PIR	TVHURG. S25722.
RefSeq	NP_001656.2.
UniGene	Hs.501728 Hs.705620
3D structure databases
HSSP	HSSP built from PDB template 1AM4 based on UniProtKB P21181.
SMR	P84095. Positions 1-177.
ModBase	Search...
Protein-protein interaction databases
IntAct	P84095.
PTM databases
PhosphoSite	P84095.
Proteomic databases
PeptideAtlas	P84095.
Genome annotation databases
Ensembl	ENSG00000177105. Homo sapiens. [Contig view]
GeneID	391.
KEGG	hsa:391.
Organism-specific databases
H-InvDB	HIX0035905.
HGNC	HGNC:672. RHOG.
MIM	179505. gene.
PharmGKB	PA24955.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P84095.
HOVERGEN	P84095.
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
CleanEx	HS_RHOG.
GermOnline	ENSG00000177105. Homo sapiens.
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_trnsfrmng. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	P84095.
NextBio	1631.
SOURCE	Search...

Entry information

Entry name

RHOG_HUMAN

Accession

Primary (citable) accession number: P84095
Secondary accession number(s): P35238, Q8NI04

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)