P84095 (RHOG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-related GTP-binding protein RhoG

Gene names

Name:	RHOG
Synonyms:	ARHG

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Ref.4 Ref.5 Ref.6 Ref.7
Subunit structure	Interacts with ARHGEF26. Interacts with ARHGEF16. Ref.4 Ref.7
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	ADP-ribosylation Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rac protein signal transduction Inferred from direct assay PubMed 12376551. Source: MGI Rho protein signal transduction Inferred from direct assay PubMed 12376551. Source: MGI actin cytoskeleton organization Inferred from direct assay PubMed 12545154. Source: MGI activation of Rac GTPase activity Inferred from mutant phenotype Ref.7. Source: UniProtKB axon guidance Traceable author statement. Source: Reactome cell chemotaxis Inferred from mutant phenotype Ref.7. Source: UniProtKB platelet activation Traceable author statement. Source: Reactome positive regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc positive regulation of establishment of protein localization to plasma membrane Inferred from mutant phenotype Ref.7. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 12545154. Source: MGI
Cellular_component	cytosol Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 12376551. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ELMO1	Q92556	3	EBI-446579,EBI-346417

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Rho-related GTP-binding protein RhoG		PRO_0000042028
	Propeptide	189 – 191	3	Removed in mature form By similarity		PRO_0000042029
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	39	1	ADP-ribosylasparagine; by botulinum toxin By similarity
	Modified residue	188	1	Cysteine methyl ester By similarity
	Lipidation	188	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	133	1	G → S in CAA43784. Ref.1
	Sequence conflict	169	1	F → L in AAM21121. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P84095 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 0C4FE9C54140F499	FASTA	191	21,309
	10 20 30 40 50 60 MQSIKCVVVG DGAVGKTCLL ICYTTNAFPK EYIPTVFDNY SAQSAVDGRT VNLNLWDTAG 70 80 90 100 110 120 QEEYDRLRTL SYPQTNVFVI CFSIASPPSY ENVRHKWHPE VCHHCPDVPI LLVGTKKDLR 130 140 150 160 170 180 AQPDTLRRLK EQGQAPITPQ QGQALAKQIH AVRYLECSAL QQDGVKEVFA EAVRAVLNPT 190 PIKRGRSCIL L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Growth-regulated expression of rhoG, a new member of the ras homolog gene family." Vincent S., Jeanteur P., Fort P. Mol. Cell. Biol. 12:3138-3148(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Oncogene ect2 is related to regulators of small GTP-binding proteins." Miki T., Smith C.L., Long J.E., Eva A., Fleming T.P. Nature 362:462-465(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis." Ellerbroek S.M., Wennerberg K., Arthur W.T., Dunty J.M., Bowman D.R., DeMali K.A., Der C., Burridge K. Mol. Biol. Cell 15:3309-3319(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARHGEF26, FUNCTION.
[5]	"Differential activation and function of Rho GTPases during Salmonella-host cell interactions." Patel J.C., Galan J.E. J. Cell Biol. 175:453-463(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration." van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., Burridge K. J. Cell Biol. 178:1279-1293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism." Hiramoto-Yamaki N., Takeuchi S., Ueda S., Harada K., Fujimoto S., Negishi M., Katoh H. J. Cell Biol. 190:461-477(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARHGEF16.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11317 mRNA. Translation: AAA60268.1. X61587 mRNA. Translation: CAA43784.1. AF498974 mRNA. Translation: AAM21121.1. AY563952 mRNA. Translation: AAS75333.1.
IPI	IPI00017342.
PIR	TVHURG. S25722.
RefSeq	NP_001656.2. NM_001665.3.
UniGene	Hs.501728.
3D structure databases
ProteinModelPortal	P84095.
ModBase	Search...
Protein-protein interaction databases
IntAct	P84095. 2 interactions.
STRING	9606.ENSP00000339467.
PTM databases
PhosphoSite	P84095.
Polymorphism databases
DMDM	51338611.
Proteomic databases
PaxDb	P84095.
PeptideAtlas	P84095.
PRIDE	P84095.
Protocols and materials databases
DNASU	391.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000351018; ENSP00000339467; ENSG00000177105. ENST00000396978; ENSP00000380175; ENSG00000177105. ENST00000396979; ENSP00000380176; ENSG00000177105. ENST00000533217; ENSP00000436932; ENSG00000177105.
GeneID	391.
KEGG	hsa:391.
UCSC	uc001lyu.2. human.
Organism-specific databases
CTD	391.
GeneCards	GC11M003848.
HGNC	HGNC:672. RHOG.
HPA	HPA039871.
MIM	179505. gene.
neXtProt	NX_P84095.
PharmGKB	PA24955.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233974.
HOVERGEN	HBG009351.
InParanoid	P84095.
KO	K07863.
OMA	ARSCILL.
OrthoDB	EOG49KFRK.
PhylomeDB	P84095.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P84095.
Bgee	P84095.
CleanEx	HS_RHOG.
Genevestigator	P84095.
GermOnline	ENSG00000177105. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RHOG. human.
GenomeRNAi	391.
NextBio	1631.
SOURCE	Search...

Entry information

Entry name

RHOG_HUMAN

Accession

Primary (citable) accession number: P84095
Secondary accession number(s): P35238, Q8NI04

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.