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Protein

Rho-related GTP-binding protein RhoG

Gene

RHOG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi115 – 1184GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: MGI
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. activation of Rac GTPase activity Source: UniProtKB
  3. axon guidance Source: Reactome
  4. blood coagulation Source: Reactome
  5. cell chemotaxis Source: UniProtKB
  6. GTP catabolic process Source: GOC
  7. platelet activation Source: Reactome
  8. positive regulation of cell proliferation Source: ProtInc
  9. positive regulation of establishment of protein localization to plasma membrane Source: UniProtKB
  10. positive regulation of transcription, DNA-templated Source: MGI
  11. Rac protein signal transduction Source: MGI
  12. regulation of ruffle assembly Source: Ensembl
  13. regulation of small GTPase mediated signal transduction Source: Reactome
  14. Rho protein signal transduction Source: MGI
  15. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_1695. GPVI-mediated activation cascade.
REACT_18407. G alpha (12/13) signalling events.
REACT_19277. Sema4D induced cell migration and growth-cone collapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoG
Gene namesi
Name:RHOG
Synonyms:ARHG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:672. RHOG.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: UniProtKB
  3. focal adhesion Source: UniProtKB
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24955.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Rho-related GTP-binding protein RhoGPRO_0000042028Add
BLAST
Propeptidei189 – 1913Removed in mature formBy similarityPRO_0000042029

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391ADP-ribosylasparagine; by botulinum toxinBy similarity
Modified residuei188 – 1881Cysteine methyl esterBy similarity
Lipidationi188 – 1881S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP84095.
PaxDbiP84095.
PeptideAtlasiP84095.
PRIDEiP84095.

PTM databases

PhosphoSiteiP84095.

Expressioni

Gene expression databases

BgeeiP84095.
CleanExiHS_RHOG.
ExpressionAtlasiP84095. baseline and differential.
GenevestigatoriP84095.

Organism-specific databases

HPAiHPA039871.

Interactioni

Subunit structurei

Interacts with ARHGEF26. Interacts with ARHGEF16.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELMO1Q925563EBI-446579,EBI-346417

Protein-protein interaction databases

BioGridi106884. 12 interactions.
IntActiP84095. 2 interactions.
MINTiMINT-4132365.
STRINGi9606.ENSP00000339467.

Structurei

3D structure databases

ProteinModelPortaliP84095.
SMRiP84095. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP84095.
KOiK07863.
OMAiRQIRAVK.
OrthoDBiEOG764747.
PhylomeDBiP84095.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84095-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSIKCVVVG DGAVGKTCLL ICYTTNAFPK EYIPTVFDNY SAQSAVDGRT
60 70 80 90 100
VNLNLWDTAG QEEYDRLRTL SYPQTNVFVI CFSIASPPSY ENVRHKWHPE
110 120 130 140 150
VCHHCPDVPI LLVGTKKDLR AQPDTLRRLK EQGQAPITPQ QGQALAKQIH
160 170 180 190
AVRYLECSAL QQDGVKEVFA EAVRAVLNPT PIKRGRSCIL L
Length:191
Mass (Da):21,309
Last modified:August 16, 2004 - v1
Checksum:i0C4FE9C54140F499
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331G → S in CAA43784. (PubMed:1620121)Curated
Sequence conflicti169 – 1691F → L in AAM21121. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11317 mRNA. Translation: AAA60268.1.
X61587 mRNA. Translation: CAA43784.1.
AF498974 mRNA. Translation: AAM21121.1.
AY563952 mRNA. Translation: AAS75333.1.
CCDSiCCDS7748.1.
PIRiS25722. TVHURG.
RefSeqiNP_001656.2. NM_001665.3.
XP_005252973.1. XM_005252916.1.
UniGeneiHs.501728.

Genome annotation databases

EnsembliENST00000351018; ENSP00000339467; ENSG00000177105.
ENST00000396978; ENSP00000380175; ENSG00000177105.
ENST00000396979; ENSP00000380176; ENSG00000177105.
ENST00000533217; ENSP00000436932; ENSG00000177105.
GeneIDi391.
KEGGihsa:391.
UCSCiuc001lyu.2. human.

Polymorphism databases

DMDMi51338611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11317 mRNA. Translation: AAA60268.1.
X61587 mRNA. Translation: CAA43784.1.
AF498974 mRNA. Translation: AAM21121.1.
AY563952 mRNA. Translation: AAS75333.1.
CCDSiCCDS7748.1.
PIRiS25722. TVHURG.
RefSeqiNP_001656.2. NM_001665.3.
XP_005252973.1. XM_005252916.1.
UniGeneiHs.501728.

3D structure databases

ProteinModelPortaliP84095.
SMRiP84095. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106884. 12 interactions.
IntActiP84095. 2 interactions.
MINTiMINT-4132365.
STRINGi9606.ENSP00000339467.

PTM databases

PhosphoSiteiP84095.

Polymorphism databases

DMDMi51338611.

Proteomic databases

MaxQBiP84095.
PaxDbiP84095.
PeptideAtlasiP84095.
PRIDEiP84095.

Protocols and materials databases

DNASUi391.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000351018; ENSP00000339467; ENSG00000177105.
ENST00000396978; ENSP00000380175; ENSG00000177105.
ENST00000396979; ENSP00000380176; ENSG00000177105.
ENST00000533217; ENSP00000436932; ENSG00000177105.
GeneIDi391.
KEGGihsa:391.
UCSCiuc001lyu.2. human.

Organism-specific databases

CTDi391.
GeneCardsiGC11M003848.
HGNCiHGNC:672. RHOG.
HPAiHPA039871.
MIMi179505. gene.
neXtProtiNX_P84095.
PharmGKBiPA24955.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP84095.
KOiK07863.
OMAiRQIRAVK.
OrthoDBiEOG764747.
PhylomeDBiP84095.
TreeFamiTF101109.

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_1695. GPVI-mediated activation cascade.
REACT_18407. G alpha (12/13) signalling events.
REACT_19277. Sema4D induced cell migration and growth-cone collapse.

Miscellaneous databases

ChiTaRSiRHOG. human.
GeneWikiiRhoG.
GenomeRNAii391.
NextBioi1631.
PROiP84095.
SOURCEiSearch...

Gene expression databases

BgeeiP84095.
CleanExiHS_RHOG.
ExpressionAtlasiP84095. baseline and differential.
GenevestigatoriP84095.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Growth-regulated expression of rhoG, a new member of the ras homolog gene family."
    Vincent S., Jeanteur P., Fort P.
    Mol. Cell. Biol. 12:3138-3148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Oncogene ect2 is related to regulators of small GTP-binding proteins."
    Miki T., Smith C.L., Long J.E., Eva A., Fleming T.P.
    Nature 362:462-465(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis."
    Ellerbroek S.M., Wennerberg K., Arthur W.T., Dunty J.M., Bowman D.R., DeMali K.A., Der C., Burridge K.
    Mol. Biol. Cell 15:3309-3319(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARHGEF26, FUNCTION.
  5. "Differential activation and function of Rho GTPases during Salmonella-host cell interactions."
    Patel J.C., Galan J.E.
    J. Cell Biol. 175:453-463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration."
    van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., Burridge K.
    J. Cell Biol. 178:1279-1293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism."
    Hiramoto-Yamaki N., Takeuchi S., Ueda S., Harada K., Fujimoto S., Negishi M., Katoh H.
    J. Cell Biol. 190:461-477(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARHGEF16.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHOG_HUMAN
AccessioniPrimary (citable) accession number: P84095
Secondary accession number(s): P35238, Q8NI04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.