Reviewed, UniProtKB/Swiss-Prot P84094 (KAX6D_HETSP)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 6.13 Alternative name(s):     Spinoxin
Organism	Heterometrus spinifer (Asia giant forest scorpion) (Malaysian black scorpion)
Taxonomic identifier	118530 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Scorpionoidea › Scorpionidae › Scorpioninae › Heterometrus

Protein attributes

Sequence length	34 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Antagonist of Kv1 potassium channels. Ref.1 Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 6 subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 34

34

Potassium channel toxin alpha-KTx 6.13

PRO_0000248273

Amino acid modifications

Modified residue

34

1

Cysteine amide

Disulfide bond

3 ↔ 24

Ref.3

Disulfide bond

9 ↔ 29

Ref.3

Disulfide bond

13 ↔ 19

Ref.3

Disulfide bond

31 ↔ 34

Ref.3

Secondary structure

1

.

.

.

.

.

.

34

Helix Strand Turn

Details...

Helix

7 – 9

3

Helix

10 – 16

7

Beta strand

21 – 31

11

Sequences

	Sequence		Length	Mass (Da)
	P84094-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: BE9142E067B6BD26	FASTA	34	3,709
	10 20 30 IRCSGSRDCY SPCMKQTGCP NAKCINKSCK CYGC

References

[1]	Nirthanan S., Huys I., Sugahara Y., Gopalakrishnakone P., Sato K., Tytgat J. Submitted (JUL-2004) to UniProtKB Cited for: PROTEIN SEQUENCE, FUNCTION. Tissue: Venom.
[2]	"Synthesis and characterization of spinoxin, a novel peptide toxin from the Malaysian black scorpion." Sugahara Y., Nirthanan S., Huys I., Kobayashi K., Kohno T., Tytgat J., Gopalakrishnakone P., Sato K. (In) Ueki M. (eds.); Peptide science 2003 - The Proceedings of the 40th Symposium on Japanese peptide symposium, pp.283-284, Protein Research Foundation, Osaka (2004) Cited for: SYNTHESIS, FUNCTION.
[3]	"Three-dimensional solution structure of spinoxin, a potassium channel blocker." Kobayashi K., Sugahara Y., Nirthanan S., Huys I., Gopalakrishnakone P., Tytgat J., Sato K., Kohno T. Submitted (NOV-2003) to the PDB data bank Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V56	NMR	-	A	1-34	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR001947. Scorpion_toxinS.
[Graphical view]

Pfam

PF00451. Toxin_2. 1 hit.
[Graphical view]

ProDom

PD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KAX6D_HETSP

Accession

Primary (citable) accession number: P84094

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)